FastQCFastQC Report
Thu 26 May 2016
SRR936587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936587_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2367603
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT143240.6050000781381001No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78570.33185462258664145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70970.2997546463659659No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58540.24725429052083478No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47760.20172300846045557No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41820.17663434283534868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40940.17291750348348095No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40150.16958079542896337No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35570.1502363360749247No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC34930.14753318018265732No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27640.11674254509729882No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27530.11627794017831537No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27240.1150530726646317No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG1351.3442772E-524.6278155
GTACCTG23200.024.165871
GTACATG105000.024.1182791
TACCTGG22500.023.64672
TACATGG107400.023.1330262
ACATGGG106250.022.5315343
GAGTACT95500.020.2927712-13
AGTACTT98400.020.12915412-13
TAAGGTG10050.019.8492875
GTACTTT98900.019.30718214-15
AGAGTAC155600.019.10929710-11
CATGGGG51150.018.3857444
CATGGGA56350.017.9534244
TACTTTT109400.017.82317214-15
ACTTTTT113500.017.03196716-17
AGTACAT81000.016.5386142
GAGTACA86500.016.3135621
GTATCAA262750.015.2980491
ATACCGT2801.340334E-515.26608856
GTAAGGT9400.015.1584594
Produced by FastQC (version 0.11.5)