##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936587_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2367603 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.911346623568225 31.0 31.0 34.0 30.0 34.0 2 32.03283616383321 33.0 31.0 34.0 30.0 34.0 3 32.15472991037771 33.0 31.0 34.0 30.0 34.0 4 35.883031065596725 37.0 35.0 37.0 35.0 37.0 5 35.749102784546224 37.0 35.0 37.0 35.0 37.0 6 35.79855490975472 37.0 35.0 37.0 35.0 37.0 7 35.72557265724026 37.0 35.0 37.0 33.0 37.0 8 35.69274916445029 37.0 35.0 37.0 33.0 37.0 9 37.30404632871305 39.0 37.0 39.0 34.0 39.0 10-11 37.19795675203993 39.0 37.0 39.0 33.0 39.0 12-13 37.07744330447292 39.0 37.0 39.0 33.0 39.0 14-15 38.157724500264614 40.0 37.0 41.0 33.0 41.0 16-17 38.13676870657792 40.0 37.5 41.0 33.0 41.0 18-19 38.27900792489282 40.0 38.0 41.0 33.5 41.0 20-21 38.28988728262297 40.0 38.0 41.0 34.0 41.0 22-23 38.18816288034776 40.0 37.5 41.0 33.0 41.0 24-25 38.07027001570787 40.0 37.0 41.0 33.0 41.0 26-27 37.87339368128863 40.0 37.0 41.0 33.0 41.0 28-29 37.90590905654369 40.0 37.0 41.0 33.0 41.0 30-31 37.79988959297653 40.0 37.0 41.0 33.0 41.0 32-33 37.619959300609096 40.0 37.0 41.0 33.0 41.0 34-35 37.43727728001696 39.5 36.0 41.0 32.0 41.0 36-37 37.268732975925445 39.0 36.0 41.0 31.5 41.0 38-39 37.074897269516896 39.0 36.0 41.0 31.0 41.0 40-41 36.87998220140793 39.0 35.5 41.0 31.0 41.0 42-43 36.61775538382068 39.0 35.0 40.0 30.0 41.0 44-45 36.33956241819258 39.0 35.0 40.0 30.0 41.0 46-47 35.97902372146006 38.5 35.0 40.0 29.5 41.0 48-49 35.59442039058068 38.0 35.0 40.0 28.0 41.0 50-51 35.29117782837748 38.0 34.0 40.0 27.0 41.0 52-53 35.03535368894194 38.0 34.0 40.0 26.5 41.0 54-55 34.70903631225336 38.0 34.0 40.0 26.0 41.0 56-57 34.35164341319047 38.0 33.0 40.0 25.5 41.0 58-59 33.97922307920712 37.0 33.0 40.0 24.5 41.0 60-61 33.575881598392975 36.5 32.5 39.0 23.0 40.5 62-63 33.25300440994542 36.0 32.0 39.0 23.0 40.0 64-65 32.93690031648042 36.0 32.0 39.0 22.0 40.0 66-67 32.47083611568324 35.0 31.0 38.5 21.5 40.0 68-69 31.997252917824483 35.0 31.0 38.0 20.0 40.0 70-71 31.472408380965895 35.0 30.5 37.5 18.5 39.5 72-73 30.89941134556765 34.0 30.0 37.0 17.0 39.0 74-75 30.35659483452251 34.0 29.0 36.0 13.5 39.0 76-77 28.980419859241607 32.5 28.0 34.5 12.0 36.5 78-79 29.7747793865779 34.0 29.0 35.0 10.5 37.0 80-81 29.60851565908643 34.0 29.0 35.0 7.5 37.0 82-83 29.187379598691166 34.0 29.0 35.0 6.5 36.5 84-85 28.728492487972012 33.0 28.5 35.0 2.0 36.0 86-87 28.313517722354632 33.0 27.5 35.0 2.0 35.5 88-89 27.798808541803673 33.0 26.5 35.0 2.0 35.0 90-91 27.199277285930116 32.0 26.0 34.0 2.0 35.0 92-93 26.591675420245707 32.0 25.0 34.0 2.0 35.0 94-95 25.81484923781563 32.0 23.5 34.0 2.0 35.0 96-97 24.943029511282084 31.0 19.5 34.0 2.0 35.0 98-99 23.770177686039425 31.0 6.0 34.0 2.0 35.0 100-101 19.967279565028427 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21630605914571177 1101 2 0.32492600580967235 1101 3 0.23596566018348142 1101 4 0.1548620857854459 1101 5 0.1937516417710654 1101 6 0.1564970507024981 1101 7 0.1808083753950811 1101 8 0.13011141176456675 1101 9 0.2880729840019214 1101 10-11 0.17778094720149085 1101 12-13 0.26545261245011176 1101 14-15 0.31148435809824093 1101 16-17 0.3255668731759229 1101 18-19 0.304055276710983 1101 20-21 0.2534809015773547 1101 22-23 0.20994726938518937 1101 24-25 0.3171695607224976 1101 26-27 0.15631716446120691 1101 28-29 0.23490375328891844 1101 30-31 0.4988678636013262 1101 32-33 0.40424131352037307 1101 34-35 0.28538595995859595 1101 36-37 0.30727342196707497 1101 38-39 0.33544736757708193 1101 40-41 0.41968629339198316 1101 42-43 0.4255234515916513 1101 44-45 0.42766383982185374 1101 46-47 0.31722673400070533 1101 48-49 0.4388662186756278 1101 50-51 0.4789673197392901 1101 52-53 0.43353611877713405 1101 54-55 0.4182858319460152 1101 56-57 0.3063962148054742 1101 58-59 0.3012468069904415 1101 60-61 0.22319913642582634 1101 62-63 0.2212745056534331 1101 64-65 0.35060123753535066 1101 66-67 0.4530551200438495 1101 68-69 0.33450929152263953 1101 70-71 0.2631267267373545 1101 72-73 0.4360120902331559 1101 74-75 0.5726658321615474 1101 76-77 0.43342454296342225 1101 78-79 0.4697729037211964 1101 80-81 0.6344040936708701 1101 82-83 0.47673925220087554 1101 84-85 0.3797911675900423 1101 86-87 0.3440777986387431 1101 88-89 0.28466111199814037 1101 90-91 0.36216768782799136 1101 92-93 0.521238171234554 1101 94-95 0.33100018139170473 1101 96-97 0.4073745865091922 1101 98-99 9.156737904483236E-4 1101 100-101 0.006940502983646013 1102 1 0.19652463693989475 1102 2 0.3494769288234352 1102 3 0.3227366751784473 1102 4 0.18426725888113538 1102 5 0.0836284351598735 1102 6 0.09120621002736584 1102 7 0.214510336681613 1102 8 0.1166626436423499 1102 9 0.15772012569140514 1102 10-11 0.33035836095498183 1102 12-13 0.3085052436325739 1102 14-15 0.2738731011346758 1102 16-17 0.4188875945617383 1102 18-19 0.2936532449883913 1102 20-21 0.22728254684326288 1102 22-23 0.2534952129911474 1102 24-25 0.18868495307397382 1102 26-27 0.3400526487938933 1102 28-29 0.2897854998019582 1102 30-31 0.36840577289324017 1102 32-33 0.319177996396121 1102 34-35 0.3623298258061496 1102 36-37 0.4507035640821826 1102 38-39 0.3516015208565051 1102 40-41 0.5199894132952565 1102 42-43 0.5401298947964577 1102 44-45 0.4253511263007681 1102 46-47 0.32814149883088106 1102 48-49 0.45611975837029917 1102 50-51 0.6075385599789982 1102 52-53 0.5893081093304176 1102 54-55 0.6913993131071976 1102 56-57 0.6506317575209977 1102 58-59 0.6906226380041502 1102 60-61 0.5285008626743704 1102 62-63 0.4753834329536275 1102 64-65 0.42781394691314034 1102 66-67 0.4644425974911215 1102 68-69 0.3138248187196204 1102 70-71 0.1094056686144178 1102 72-73 0.12648278770084787 1102 74-75 0.20474945897847618 1102 76-77 0.22791367433985954 1102 78-79 0.38230223638658245 1102 80-81 0.4292736551216265 1102 82-83 0.4908919101264395 1102 84-85 0.27176927214428304 1102 86-87 0.423052351256743 1102 88-89 0.26588491034760153 1102 90-91 0.2603654474338519 1102 92-93 0.3704823960423589 1102 94-95 0.35033243244541623 1102 96-97 0.4057149132270581 1102 98-99 0.3596701796268462 1102 100-101 -0.039437124714677196 1103 1 0.22302940779597336 1103 2 0.30179859209947324 1103 3 0.2777957565485991 1103 4 0.20779228414364326 1103 5 0.23555747974224772 1103 6 0.177357113192123 1103 7 0.24109695324943203 1103 8 0.2798892048760493 1103 9 0.2152428164788418 1103 10-11 0.24764377406987848 1103 12-13 0.17428202095499756 1103 14-15 0.31191885288753696 1103 16-17 0.34005479465204047 1103 18-19 0.28859432410002483 1103 20-21 0.24903137305271628 1103 22-23 0.1638455393843472 1103 24-25 0.2670824461344665 1103 26-27 0.17385436605601257 1103 28-29 0.3413299096596063 1103 30-31 0.3809149270418857 1103 32-33 0.27489960007992664 1103 34-35 0.22866013156139786 1103 36-37 0.32568123036673313 1103 38-39 0.213949471049375 1103 40-41 0.2953569032156622 1103 42-43 0.46766675077635256 1103 44-45 0.3898530897954444 1103 46-47 0.3979713373166689 1103 48-49 0.43412639803680264 1103 50-51 0.4202172631363652 1103 52-53 0.4060500107507323 1103 54-55 0.45063062674156384 1103 56-57 0.45819958381122916 1103 58-59 0.305953744028578 1103 60-61 0.2403638369047414 1103 62-63 0.44442235032209254 1103 64-65 0.4456092374145939 1103 66-67 0.29732027399300875 1103 68-69 0.48356607125857565 1103 70-71 0.26611158734323226 1103 72-73 0.4745516702287169 1103 74-75 0.46758332127761193 1103 76-77 0.4211322756768894 1103 78-79 0.3806960811611084 1103 80-81 0.43422679176491386 1103 82-83 0.317659443162956 1103 84-85 0.5178289761837682 1103 86-87 0.46622716781268636 1103 88-89 0.45201195521991266 1103 90-91 0.557731312743627 1103 92-93 0.5269221824731964 1103 94-95 0.522950922836035 1103 96-97 0.7446352639760541 1103 98-99 0.6454997447586486 1103 100-101 0.7285996908773065 1104 1 0.17450475706595725 1104 2 0.28690142320468937 1104 3 0.2208845210701611 1104 4 0.16159532460559234 1104 5 0.08417791460219348 1104 6 0.10209103856259816 1104 7 0.10515313748336297 1104 8 0.137893884614968 1104 9 0.29218169252049364 1104 10-11 0.21439368170660345 1104 12-13 0.3196628997059676 1104 14-15 0.2610014461744399 1104 16-17 0.22191451195201495 1104 18-19 0.16681958100674876 1104 20-21 0.2525089527245896 1104 22-23 0.16798341379767834 1104 24-25 0.09864988500366678 1104 26-27 0.14182237415661092 1104 28-29 0.19451808442459395 1104 30-31 0.2513478713091999 1104 32-33 0.28242645384492704 1104 34-35 0.22262833123026127 1104 36-37 0.15524315480673323 1104 38-39 0.22542919902244307 1104 40-41 0.23190320115904228 1104 42-43 0.12950580607116535 1104 44-45 0.06858107654986156 1104 46-47 0.0544261614159538 1104 48-49 0.2048806296428225 1104 50-51 0.15522225759456632 1104 52-53 0.1884490611194991 1104 54-55 0.04871795450117844 1104 56-57 0.34286119216361044 1104 58-59 0.4017436894316333 1104 60-61 0.20788015441544871 1104 62-63 0.20485543142387996 1104 64-65 0.14278497392614042 1104 66-67 0.10755679237671956 1104 68-69 0.1434661136772668 1104 70-71 -0.0576989790146456 1104 72-73 0.24108462681113707 1104 74-75 0.41999065013189707 1104 76-77 0.22541943649844143 1104 78-79 0.3385970403707539 1104 80-81 0.38680502687390117 1104 82-83 0.22623335430264646 1104 84-85 0.2507282933797583 1104 86-87 0.3056036131267881 1104 88-89 0.3258806762281097 1104 90-91 0.20003047394831697 1104 92-93 0.17383916499943552 1104 94-95 0.3175705914229461 1104 96-97 0.4893548901442806 1104 98-99 0.09678831497525664 1104 100-101 0.09552720965783479 1105 1 0.06309373571671628 1105 2 0.10299167788198815 1105 3 0.16395367290851937 1105 4 0.04356445356347649 1105 5 0.1061333152745263 1105 6 -0.003869501416630783 1105 7 -0.024658010588517243 1105 8 0.010272222084388716 1105 9 0.14351773631020137 1105 10-11 0.1726896142620049 1105 12-13 0.04317769281366424 1105 14-15 0.08209618894508708 1105 16-17 0.1515098795356593 1105 18-19 0.09777228882306588 1105 20-21 0.005494150816709009 1105 22-23 -0.09318489850321754 1105 24-25 0.0074736525380387775 1105 26-27 0.044014675756493205 1105 28-29 0.07582579162490077 1105 30-31 0.1349105374126509 1105 32-33 0.20649073038187282 1105 34-35 0.00340556339669007 1105 36-37 0.16177676270577734 1105 38-39 0.02588680854072578 1105 40-41 0.23188595280027613 1105 42-43 0.3919955538579174 1105 44-45 0.33202441217124345 1105 46-47 0.34486916263038125 1105 48-49 0.06349900090494742 1105 50-51 0.2211004278605131 1105 52-53 0.22492300377750496 1105 54-55 0.24194814968924305 1105 56-57 0.2503737324244426 1105 58-59 0.40854623092449316 1105 60-61 0.051882477663774296 1105 62-63 0.2695434649349764 1105 64-65 0.07320544667197026 1105 66-67 0.20324399867060805 1105 68-69 0.1771855990425628 1105 70-71 -0.1706405281988701 1105 72-73 7.79084455853507E-4 1105 74-75 0.04486947161138488 1105 76-77 -0.12318960656965317 1105 78-79 -0.048004152934069566 1105 80-81 0.025139680234694595 1105 82-83 -0.013833527088483066 1105 84-85 0.082374103612608 1105 86-87 0.08336836663366043 1105 88-89 0.12520130209712477 1105 90-91 0.2740069246584902 1105 92-93 0.09818610341575607 1105 94-95 0.034037493934523155 1105 96-97 0.2277331924129662 1105 98-99 0.12288543379532868 1105 100-101 -0.29946126210237267 1106 1 0.1009130154408453 1106 2 0.18782168507306096 1106 3 0.08220796573453981 1106 4 0.10683075916176676 1106 5 0.08346289511240457 1106 6 0.058643797021602495 1106 7 0.0987664986405079 1106 8 0.11059643350052539 1106 9 0.11069989552217407 1106 10-11 0.19251786515764024 1106 12-13 0.10548506831684534 1106 14-15 0.19187886271195254 1106 16-17 0.24414440202887278 1106 18-19 0.2103562357779012 1106 20-21 0.14701766317677567 1106 22-23 0.12991907779291978 1106 24-25 0.10242879307412522 1106 26-27 -0.05104403932767099 1106 28-29 0.13185637640997072 1106 30-31 0.18878502612151493 1106 32-33 0.19458658155765818 1106 34-35 0.09715279101040863 1106 36-37 0.1750093488742479 1106 38-39 0.33197376067211337 1106 40-41 0.14980954276912684 1106 42-43 0.27313387853239135 1106 44-45 0.41757172359668715 1106 46-47 0.35921503093634755 1106 48-49 0.26915549836859753 1106 50-51 0.3363026614932423 1106 52-53 0.3166308369994084 1106 54-55 0.21623374857156108 1106 56-57 0.14559062228849484 1106 58-59 0.08730262950185619 1106 60-61 0.1414089703165402 1106 62-63 0.31071124632632063 1106 64-65 0.26149517555332835 1106 66-67 0.060355913277867046 1106 68-69 0.143420440626155 1106 70-71 -0.0016481945907322881 1106 72-73 0.06255690262482361 1106 74-75 0.23560553611034152 1106 76-77 0.022630302102982114 1106 78-79 -0.08287509226722634 1106 80-81 0.10078098051361195 1106 82-83 0.15227386586945713 1106 84-85 0.2527439210083742 1106 86-87 0.16913840661372603 1106 88-89 0.1682123642339235 1106 90-91 0.27931194665365666 1106 92-93 0.16834379494391882 1106 94-95 0.12684014475053118 1106 96-97 0.2207459495371431 1106 98-99 0.3128438957854165 1106 100-101 0.061968398445745976 1107 1 0.08697860359628606 1107 2 0.212317881447305 1107 3 0.09139261991893477 1107 4 0.09784844196962439 1107 5 -0.030748078365967046 1107 6 0.12555333690108483 1107 7 -0.024315300217942593 1107 8 0.08092102952335978 1107 9 0.05577779010253181 1107 10-11 0.080325616109711 1107 12-13 0.12751192752023854 1107 14-15 0.15290576424093416 1107 16-17 0.1963639665707575 1107 18-19 0.17698508469720053 1107 20-21 0.08970003229519108 1107 22-23 0.12282850824525582 1107 24-25 0.17331900357686436 1107 26-27 0.04856099060477703 1107 28-29 0.1257274237413455 1107 30-31 0.11268773506284901 1107 32-33 0.0852079522668987 1107 34-35 0.10657436529544384 1107 36-37 0.16125094956632324 1107 38-39 0.10657602911756214 1107 40-41 0.12723296699237352 1107 42-43 0.08416506423748871 1107 44-45 -0.032853648636283594 1107 46-47 0.07812352609626316 1107 48-49 -0.05250715424872254 1107 50-51 0.03656053792521874 1107 52-53 -0.17234437775137224 1107 54-55 -0.055747946124633074 1107 56-57 -0.008895219799875065 1107 58-59 0.13050260968109484 1107 60-61 0.18567070353935122 1107 62-63 -0.09446599984656956 1107 64-65 0.05075139096377512 1107 66-67 0.1530489670282762 1107 68-69 0.09107813185783797 1107 70-71 -0.05544763940279651 1107 72-73 -0.004484775698589516 1107 74-75 -0.08125780693034912 1107 76-77 -0.35335312278095543 1107 78-79 -0.11880074787520911 1107 80-81 0.19684975857366993 1107 82-83 0.25558548238717194 1107 84-85 0.19190139390192584 1107 86-87 0.4766529547467009 1107 88-89 0.3253667834906224 1107 90-91 0.15670708499125396 1107 92-93 0.2520834367867977 1107 94-95 0.5808384166274436 1107 96-97 0.6483222104527577 1107 98-99 0.81512586477211 1107 100-101 0.5678960464537077 1108 1 0.04117542631743021 1108 2 0.1704247587563188 1108 3 -0.018604209001310323 1108 4 0.009450421390987174 1108 5 0.10822076637153089 1108 6 0.008987395728190961 1108 7 0.04996694495420684 1108 8 0.13036617997678945 1108 9 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sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 24.0 5 71.0 6 125.0 7 356.0 8 847.0 9 1601.0 10 2722.0 11 4213.0 12 6307.0 13 8591.0 14 11188.0 15 14667.0 16 18790.0 17 23741.0 18 21696.0 19 15758.0 20 15005.0 21 16129.0 22 17327.0 23 19601.0 24 22870.0 25 27337.0 26 33428.0 27 41443.0 28 50952.0 29 62696.0 30 77647.0 31 95315.0 32 119002.0 33 151591.0 34 198306.0 35 265923.0 36 366371.0 37 437383.0 38 211305.0 39 7266.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.61685410724665 19.26999553927466 14.691584774057503 22.421565579421188 2 15.886801937183058 21.005172439283506 38.86400645446078 24.244019169072654 3 20.474954677317708 24.371649373930293 29.663463244276162 25.489932704475837 4 13.28451312912364 16.912112228464366 36.860042600119364 32.94333204229263 5 13.687515417341087 37.79229589129972 33.785772455251696 14.734416236107498 6 31.92807240065163 36.326994010397854 16.95516520294999 14.789768386000524 7 28.842897539486273 32.747154813217634 20.233866011712305 18.176081635583788 8 26.804809530869267 34.06822836612886 19.205389202081985 19.921572900919895 9 26.20445759230546 17.22177850068487 20.424683044144682 36.149080862864984 10-11 25.460053902617965 26.483240644651996 26.71765494468456 21.339050508045478 12-13 26.80707027318347 23.837801354365578 27.21440207669951 22.140726295751442 14-15 23.06956409535674 25.541462248481423 26.3080000853187 25.080973570843135 16-17 22.20496307398782 28.64034682933021 26.788988151870463 22.36570194481151 18-19 21.984743786852288 27.462260724121034 29.021862170335705 21.531133318690973 20-21 23.14378931616197 25.961617859207198 29.00556284028602 21.88902998434481 22-23 22.497959425027744 26.136212207126015 29.13305393559069 22.23277443225555 24-25 22.910671648946124 26.2341341895623 29.110074332940105 21.745119828551466 26-27 22.57001442176819 26.124962330020484 29.503170191436357 21.801853056774966 28-29 21.950641588392976 27.132406839751894 29.108437001935084 21.80851456992005 30-31 22.36587960853069 26.271977489374233 29.025744712034545 22.33639819006054 32-33 22.245894573045522 26.456506032823984 29.168705555824236 22.12889383830625 34-35 23.048348020598215 26.292414545798586 28.889217869355427 21.77001956424777 36-37 22.77860738912334 25.99694328520602 29.183558954533428 22.040890371137213 38-39 22.383896946812353 26.612492246491147 29.16575940669316 21.837851400003334 40-41 22.642809611330286 26.560846493803965 28.79754046447428 21.998803430391476 42-43 22.34599482969481 26.721261586566918 28.284306385671147 22.64843719806712 44-45 22.717841841667642 26.324065043466362 28.332121769168094 22.625971345697906 46-47 23.215378591765596 26.981466065045534 27.842885821651684 21.960269521537185 48-49 23.191653504361987 26.90575705373874 27.712432628323757 22.190156813575516 50-51 22.405050726902576 27.199017562236545 28.111257064175234 22.284674646685644 52-53 23.111581819487327 27.466896717759408 27.193116206775663 22.2284052559776 54-55 22.623664761010147 26.9769540933954 27.54647585181706 22.852905293777393 56-57 23.039324707154076 27.091170490280497 27.36722005416654 22.502284748398882 58-59 23.631031714739045 26.804258479779907 27.195294223986437 22.369415581494607 60-61 22.94157400661028 27.124085278930526 28.038521240536003 21.89581947392319 62-63 22.637950379698932 27.185193272764245 27.909048806607707 22.267807540929113 64-65 22.449672210984534 27.61529003446209 27.471617031934986 22.463420722618395 66-67 22.791023294957178 27.157816708886422 27.139739120977286 22.91142087517911 68-69 22.642568162460897 27.087185311631952 27.493848626043558 22.776397899863593 70-71 23.098286180361097 27.03133424667776 27.280670093784998 22.589709479176147 72-73 23.00772912947084 26.78885271909161 27.725606822617767 22.477811328819783 74-75 22.5281625263631 27.847710945971123 27.077483960380484 22.546642567285303 76-77 22.781584309299667 27.209419117001076 27.101586944915628 22.90740962878363 78-79 22.865404346215467 27.304082597270725 27.140107939423146 22.690405117090663 80-81 23.220556544393375 27.309614460253645 26.97661030150308 22.493218693849897 82-83 23.056291322196238 27.259545102624134 27.285965120507804 22.398198454671824 84-85 22.739341201097673 27.51843571690747 27.129700690713737 22.612522391281118 86-87 22.745851266477725 27.57092655400639 26.885989550555628 22.797232628960252 88-89 22.677064249246314 27.551812801275588 26.5843096466265 23.18681330285159 90-91 22.871514722970925 27.076339969663216 26.911688363254054 23.140456944111804 92-93 23.170589025392623 27.265623614066598 27.051857245797112 22.51193011474367 94-95 22.795194533863576 27.084356259335685 27.15168194904915 22.968767257751587 96-97 22.356997652898443 27.147986506172284 27.60253944815871 22.892476392770572 98-99 22.487902775334483 27.500239167405994 26.670196277773776 23.34166177948575 100-101 22.277745970474612 27.377204040200958 26.775739787303827 23.569310202020606 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 254.0 1 204.0 2 217.5 3 278.0 4 249.5 5 245.5 6 408.5 7 802.0 8 1293.5 9 1675.5 10 1700.5 11 1601.0 12 1731.5 13 1944.0 14 2123.0 15 2394.0 16 2656.0 17 2922.0 18 3213.5 19 3514.5 20 3833.0 21 4301.5 22 5097.5 23 5942.0 24 7011.0 25 8767.5 26 11194.5 27 14144.0 28 16985.5 29 20761.0 30 25903.0 31 30991.0 32 36444.0 33 41402.0 34 45962.5 35 50133.0 36 55318.0 37 60698.5 38 63465.5 39 65052.0 40 67126.5 41 70569.0 42 73414.5 43 76372.5 44 80154.5 45 83789.0 46 85760.5 47 94331.5 48 114417.5 49 113737.5 50 94545.5 51 84430.0 52 79998.0 53 77732.5 54 75590.5 55 71548.0 56 66030.0 57 59633.5 58 54570.5 59 49318.5 60 41605.5 61 34142.5 62 27970.0 63 22126.0 64 16353.5 65 12149.0 66 9265.5 67 7337.5 68 5433.0 69 3789.5 70 2800.5 71 2065.5 72 1689.0 73 1490.0 74 1140.5 75 865.5 76 635.5 77 360.5 78 206.0 79 135.5 80 93.5 81 63.0 82 40.5 83 25.5 84 14.5 85 8.0 86 4.5 87 4.0 88 2.0 89 1.0 90 3.0 91 2.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011615122974586533 2 0.01047472908253622 3 0.005701969460251571 4 1.6894724326671322E-4 5 0.005870916703518284 6 0.0 7 3.3789448653342644E-4 8 0.0022807877841006284 9 8.447362163335661E-5 10-11 0.0 12-13 0.0 14-15 4.012497027584439E-4 16-17 0.004160325865442813 18-19 0.001816182865117167 20-21 7.391441892918703E-4 22-23 4.4348651357512214E-4 24-25 5.068417298001397E-4 26-27 6.335521622501746E-5 28-29 1.0559202704169576E-4 30-31 4.2236810816678304E-4 32-33 0.004075852243809456 34-35 8.024994055168878E-4 36-37 0.0012037491082753317 38-39 0.005342956568309806 40-41 6.335521622501746E-5 42-43 0.0018795380813421845 44-45 0.005913153514334963 46-47 0.0 48-49 0.0019640117029755414 50-51 9.71446648783601E-4 52-53 5.49078540616818E-4 54-55 1.0559202704169576E-4 56-57 0.011594004569178194 58-59 0.0018795380813421845 60-61 0.0032944712437009073 62-63 1.4782883785837407E-4 64-65 0.0031255240004341945 66-67 0.0012882227299086883 68-69 0.0017950644597088277 70-71 0.004793878027692987 72-73 0.002787629513900768 74-75 0.007771573190268808 76-77 0.0014782883785837407 78-79 0.006926836973935242 80-81 0.0027453927030840894 82-83 0.0033789448653342644 84-85 0.002196314162467272 86-87 5.49078540616818E-4 88-89 0.002766511108492429 90-91 0.007602625947002094 92-93 0.0027453927030840894 94-95 3.167760811250873E-4 96-97 8.447362163335661E-5 98-99 6.124337568418354E-4 100-101 0.0020062485137922192 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2367603.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.095780095377236 #Duplication Level Percentage of deduplicated Percentage of total 1 78.8378479760747 45.80146279210817 2 12.758525470717355 14.824329801761296 3 3.705434122150627 6.4581025775511005 4 1.5683953278149805 3.644686002694248 5 0.7990050863662123 2.320941189630968 6 0.5094537916848094 1.7758269270286084 7 0.33658249415867475 1.368781579521717 8 0.23201858617035256 1.0783440608154509 9 0.17480933706038132 0.9140116324030733 >10 0.9569512540099159 10.3090849072848 >50 0.08028582571735982 3.1911025321004622 >100 0.0369166757451819 3.9779500761283066 >500 0.0019596040940515506 0.7504897492455506 >1k 0.0015241365175941428 2.1035554300849735 >5k 2.177337882277347E-4 0.8773633744754983 >10k+ 7.257792940924491E-5 0.6039673671658514 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14324 0.6050000781381001 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7857 0.33185462258664145 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7097 0.2997546463659659 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5854 0.24725429052083478 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4776 0.20172300846045557 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4182 0.17663434283534868 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4094 0.17291750348348095 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4015 0.16958079542896337 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3557 0.1502363360749247 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3493 0.14753318018265732 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2764 0.11674254509729882 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2753 0.11627794017831537 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2724 0.1150530726646317 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.2236810816678304E-5 0.0 0.0 0.0 6 0.0 4.2236810816678304E-5 0.0 0.0 0.0 7 0.0 4.2236810816678304E-5 0.0 0.0 0.0 8 0.0 4.2236810816678304E-5 0.0 0.0 0.0 9 0.0 4.2236810816678304E-5 0.0 0.0 0.0 10-11 0.0 4.2236810816678304E-5 0.0 0.0 0.0 12-13 0.0 4.2236810816678304E-5 0.0 0.0 0.0 14-15 0.0 4.2236810816678304E-5 0.0 0.0 0.0 16-17 0.0 1.267104324500349E-4 0.0 2.1118405408339152E-5 0.0 18-19 0.0 1.267104324500349E-4 0.0 4.2236810816678304E-5 0.0 20-21 0.0 1.267104324500349E-4 0.0 4.2236810816678304E-5 2.1118405408339152E-5 22-23 0.0 1.267104324500349E-4 0.0 4.2236810816678304E-5 4.2236810816678304E-5 24-25 0.0 1.267104324500349E-4 0.0 1.6894724326671322E-4 4.2236810816678304E-5 26-27 0.0 1.267104324500349E-4 0.0 3.8013129735010474E-4 4.2236810816678304E-5 28-29 0.0 1.267104324500349E-4 0.0 8.024994055168878E-4 4.2236810816678304E-5 30-31 8.447362163335661E-5 1.267104324500349E-4 0.0 0.0015627620002170973 4.2236810816678304E-5 32-33 8.447362163335661E-5 1.267104324500349E-4 0.0 0.0024497350273673417 4.2236810816678304E-5 34-35 1.0559202704169576E-4 1.267104324500349E-4 0.0 0.003716839351867691 4.2236810816678304E-5 36-37 1.267104324500349E-4 1.267104324500349E-4 0.0 0.0050684172980013965 4.2236810816678304E-5 38-39 1.267104324500349E-4 1.267104324500349E-4 0.0 0.006251048000868389 4.2236810816678304E-5 40-41 1.267104324500349E-4 1.267104324500349E-4 0.0 0.008088349271393895 4.2236810816678304E-5 42-43 1.267104324500349E-4 1.267104324500349E-4 0.0 0.011066044433969715 4.2236810816678304E-5 44-45 1.6894724326671322E-4 1.267104324500349E-4 0.0 0.01613446173197111 4.2236810816678304E-5 46-47 1.6894724326671322E-4 1.267104324500349E-4 0.0 0.027939650355232697 4.2236810816678304E-5 48-49 1.6894724326671322E-4 1.267104324500349E-4 0.0 0.050684172980013965 4.2236810816678304E-5 50-51 1.6894724326671322E-4 1.267104324500349E-4 0.0 0.06916277771231072 4.2236810816678304E-5 52-53 1.6894724326671322E-4 1.267104324500349E-4 0.0 0.08827493460685765 4.2236810816678304E-5 54-55 2.1118405408339152E-4 1.4782883785837407E-4 0.0 0.11401827079962307 4.2236810816678304E-5 56-57 2.1118405408339152E-4 1.6894724326671322E-4 0.0 0.13500996577551222 4.2236810816678304E-5 58-59 2.1118405408339152E-4 1.6894724326671322E-4 0.0 0.15969738169786066 4.2236810816678304E-5 60-61 2.1118405408339152E-4 1.9006564867505237E-4 0.0 0.20026583848728016 4.2236810816678304E-5 62-63 2.1118405408339152E-4 2.534208649000698E-4 0.0 0.23029621097793845 6.335521622501746E-5 64-65 2.1118405408339152E-4 2.534208649000698E-4 0.0 0.2652260535233314 8.447362163335661E-5 66-67 2.534208649000698E-4 2.9565767571674813E-4 0.0 0.3346422521005422 8.447362163335661E-5 68-69 2.534208649000698E-4 2.9565767571674813E-4 0.0 0.4147866006251893 8.447362163335661E-5 70-71 2.9565767571674813E-4 3.167760811250873E-4 0.0 0.476790238904073 8.447362163335661E-5 72-73 3.3789448653342644E-4 3.3789448653342644E-4 0.0 0.5499232768331515 8.447362163335661E-5 74-75 3.3789448653342644E-4 3.3789448653342644E-4 0.0 0.6141232292745025 8.447362163335661E-5 76-77 3.3789448653342644E-4 3.3789448653342644E-4 0.0 0.6713541079311016 8.447362163335661E-5 78-79 3.3789448653342644E-4 3.3789448653342644E-4 0.0 0.74410701456283 8.447362163335661E-5 80-81 4.434865135751222E-4 3.3789448653342644E-4 0.0 0.8617576510926874 8.447362163335661E-5 82-83 4.6460491898346135E-4 3.3789448653342644E-4 0.0 0.9768951973789525 8.447362163335661E-5 84-85 4.857233243918005E-4 3.3789448653342644E-4 0.0 1.1093920729108722 8.447362163335661E-5 86-87 5.068417298001397E-4 3.3789448653342644E-4 0.0 1.347861106781838 8.447362163335661E-5 88-89 5.068417298001397E-4 3.3789448653342644E-4 0.0 1.6410056922549938 8.447362163335661E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACCG 135 1.3442772E-5 24.627815 5 GTACCTG 2320 0.0 24.16587 1 GTACATG 10500 0.0 24.118279 1 TACCTGG 2250 0.0 23.6467 2 TACATGG 10740 0.0 23.133026 2 ACATGGG 10625 0.0 22.531534 3 GAGTACT 9550 0.0 20.29277 12-13 AGTACTT 9840 0.0 20.129154 12-13 TAAGGTG 1005 0.0 19.849287 5 GTACTTT 9890 0.0 19.307182 14-15 AGAGTAC 15560 0.0 19.109297 10-11 CATGGGG 5115 0.0 18.385744 4 CATGGGA 5635 0.0 17.953424 4 TACTTTT 10940 0.0 17.823172 14-15 ACTTTTT 11350 0.0 17.031967 16-17 AGTACAT 8100 0.0 16.538614 2 GAGTACA 8650 0.0 16.313562 1 GTATCAA 26275 0.0 15.298049 1 ATACCGT 280 1.340334E-5 15.2660885 6 GTAAGGT 940 0.0 15.158459 4 >>END_MODULE