FastQCFastQC Report
Thu 26 May 2016
SRR936586_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936586_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494015
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT118470.4750171911556266No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT78620.3152346718042995No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77770.3118265126713352No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63990.25657423872751367No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT53430.21423287349915698No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC49010.19651044600774253No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36410.1459894988602715No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35830.14366393145189585No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32750.13131436659362514No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27850.11166733159183083No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC27570.1105446438774426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG111700.026.4218881
TACATGG113800.025.6414662
GTACCTG23600.024.366821
ACATGGG115750.023.9366993
TACCTGG24050.023.3175892
GAGTACT81000.021.99718712-13
GTGTAGC13500.020.7703441
GTACTTT85950.020.72991614-15
CATGGGA65700.020.4709424
AGAGTAC142100.019.21107510-11
AGTACTT85850.019.14946712-13
CATGGGG52000.018.5527974
ACTTTTT103350.017.69902416-17
GTGTACG2453.7461523E-617.4582961
GTATCAA242550.017.2231671
ATGGGAG28300.017.1285955
TACTTTT95300.017.12560814-15
GAGTACA88300.016.8464761
GAGTACG4559.094947E-1216.7122151
GTACACG4951.8189894E-1216.3218421
Produced by FastQC (version 0.11.5)