##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936586_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2494015 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.237840189413454 31.0 31.0 34.0 28.0 34.0 2 31.28299629312574 31.0 31.0 34.0 28.0 34.0 3 31.330227765270056 31.0 31.0 34.0 28.0 34.0 4 34.9749311852575 37.0 35.0 37.0 32.0 37.0 5 34.893881151476634 37.0 35.0 37.0 32.0 37.0 6 35.00999432641744 37.0 35.0 37.0 32.0 37.0 7 34.8467936239357 37.0 35.0 37.0 32.0 37.0 8 34.828702313338134 37.0 35.0 37.0 32.0 37.0 9 36.31189948737277 39.0 35.0 39.0 32.0 39.0 10-11 36.175468672000775 39.0 35.0 39.0 32.0 39.0 12-13 36.08553316640037 39.0 35.0 39.0 30.5 39.0 14-15 37.11954338686816 39.0 36.0 41.0 31.0 41.0 16-17 37.053784560237204 39.0 36.0 41.0 31.0 41.0 18-19 37.01175935188842 39.0 36.0 41.0 31.0 41.0 20-21 36.91148168715906 39.0 36.0 41.0 30.5 41.0 22-23 36.799637131292315 39.0 36.0 41.0 30.0 41.0 24-25 36.64842713456014 39.0 36.0 41.0 30.0 41.0 26-27 36.44314528982384 39.0 35.5 40.0 30.0 41.0 28-29 36.56749277770984 39.0 36.0 41.0 30.0 41.0 30-31 36.510640473293066 39.0 36.0 41.0 30.0 41.0 32-33 36.357920261105086 39.0 35.0 40.0 30.0 41.0 34-35 36.185173906331755 39.0 35.0 40.0 29.5 41.0 36-37 35.94778740304288 38.0 35.0 40.0 28.5 41.0 38-39 35.76861025294555 38.0 35.0 40.0 27.5 41.0 40-41 35.63513210626239 38.0 35.0 40.0 27.0 41.0 42-43 35.37449233464915 38.0 34.0 40.0 26.5 41.0 44-45 35.105825145398086 38.0 34.0 40.0 26.0 41.0 46-47 34.774053083080894 38.0 33.5 40.0 24.5 41.0 48-49 34.5217971824548 38.0 33.0 40.0 24.0 41.0 50-51 33.82746154293378 37.5 32.5 39.5 23.5 40.5 52-53 34.0233356655834 37.5 33.0 39.5 23.5 40.5 54-55 34.388071843994524 38.0 34.0 40.0 23.5 41.0 56-57 34.30085885610151 38.0 33.5 40.0 23.0 41.0 58-59 34.05646938771419 38.0 33.0 40.0 23.0 41.0 60-61 33.71816368385916 37.0 33.0 40.0 22.0 41.0 62-63 33.40694061583431 37.0 32.5 40.0 22.0 41.0 64-65 33.10286545991103 36.0 32.0 39.0 20.5 41.0 66-67 32.717352742465465 36.0 32.0 39.0 20.0 41.0 68-69 32.32044213847952 35.0 31.0 39.0 18.5 40.0 70-71 31.907488527534916 35.0 31.0 38.0 17.5 40.0 72-73 31.490054189730216 35.0 31.0 37.5 14.5 39.5 74-75 31.06141222085673 35.0 30.5 37.0 12.0 39.0 76-77 30.646581516149663 35.0 30.0 36.5 8.0 39.0 78-79 30.18803455472401 34.0 30.0 36.0 7.0 38.5 80-81 29.749023562408407 34.0 29.0 35.5 4.5 37.0 82-83 29.333582797216536 34.0 29.0 35.0 2.0 37.0 84-85 28.93711705823742 34.0 29.0 35.0 2.0 36.5 86-87 28.615061256648417 34.0 29.0 35.0 2.0 36.0 88-89 28.232730556953346 34.0 27.5 35.0 2.0 36.0 90-91 27.862087637804905 34.0 27.0 35.0 2.0 35.0 92-93 27.467691252859346 33.0 26.0 35.0 2.0 35.0 94-95 27.02498180644463 33.0 25.5 35.0 2.0 35.0 96-97 26.43426663432257 33.0 25.0 35.0 2.0 35.0 98-99 25.76002810728885 33.0 23.5 35.0 2.0 35.0 100-101 23.686313434361864 31.0 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3636121381185049 1101 2 0.3071481232252431 1101 3 0.29560382047777267 1101 4 0.35035530612335464 1101 5 0.4880704892747545 1101 6 0.25866598669720275 1101 7 0.3692104492353394 1101 8 0.40162789121944087 1101 9 0.5788777098476601 1101 10-11 0.39090289768991937 1101 12-13 0.6890879734475419 1101 14-15 0.7679419912045589 1101 16-17 0.8581712563702268 1101 18-19 0.7942208667257162 1101 20-21 0.6947816567299085 1101 22-23 0.6487068267045046 1101 24-25 0.6451361495897956 1101 26-27 0.6950201538906384 1101 28-29 0.8228862789713958 1101 30-31 0.5731405417830544 1101 32-33 0.5698816610213981 1101 34-35 0.5304772435665654 1101 36-37 0.591950592774829 1101 38-39 0.671665240198422 1101 40-41 0.4843070912626857 1101 42-43 0.3360136350848748 1101 44-45 0.35300658349657965 1101 46-47 0.4518035834030272 1101 48-49 0.42412554461311913 1101 50-51 0.5692019807679998 1101 52-53 0.5859658644079531 1101 54-55 0.6897609918377441 1101 56-57 0.6071429574106446 1101 58-59 0.5514991155994338 1101 60-61 0.6392125722582804 1101 62-63 0.5760858071032118 1101 64-65 0.5979048048513746 1101 66-67 0.5876784359636744 1101 68-69 0.5661157525062634 1101 70-71 0.5244792840584225 1101 72-73 0.5445307750929906 1101 74-75 0.3829571500490232 1101 76-77 0.4130876597745399 1101 78-79 0.48895913383355705 1101 80-81 0.4006016673377992 1101 82-83 0.41461966752091683 1101 84-85 0.3296605075179144 1101 86-87 0.348151002906782 1101 88-89 0.327167449500795 1101 90-91 0.3554109915195127 1101 92-93 0.2506833441598637 1101 94-95 0.35112087125225244 1101 96-97 0.3908961979951151 1101 98-99 0.3365247054309606 1101 100-101 0.6670512041629664 1102 1 0.20334199650241658 1102 2 0.22159466370614211 1102 3 0.27703271895430603 1102 4 0.3614835452308611 1102 5 0.32012388727425645 1102 6 0.3997075991560308 1102 7 0.11046924603309094 1102 8 0.14804260993370377 1102 9 0.4130250432253746 1102 10-11 0.5203018008258056 1102 12-13 0.6277202239351638 1102 14-15 0.5103920998219351 1102 16-17 0.23406020190420662 1102 18-19 0.3809224608900337 1102 20-21 0.13409434166688072 1102 22-23 0.004712462807027862 1102 24-25 0.23783470660232098 1102 26-27 0.27568854159317624 1102 28-29 0.2296857951853326 1102 30-31 0.2663001553794828 1102 32-33 0.2832012331274072 1102 34-35 0.19681475518973457 1102 36-37 0.12810766288900766 1102 38-39 0.062133321109797635 1102 40-41 -0.12874632035180866 1102 42-43 0.13510192225834317 1102 44-45 0.11996859464272092 1102 46-47 0.29229405341273207 1102 48-49 0.25182649433446613 1102 50-51 0.27320971761339763 1102 52-53 0.18778539466046595 1102 54-55 0.1761860251692653 1102 56-57 0.2526359159383702 1102 58-59 0.1279092884190618 1102 60-61 -0.19290423630686604 1102 62-63 -0.09947970672659068 1102 64-65 0.1668816961755013 1102 66-67 0.11343188711206409 1102 68-69 0.29251454167962265 1102 70-71 0.28549444309161487 1102 72-73 0.08572904820771399 1102 74-75 -0.1158287204128996 1102 76-77 0.04608867383933557 1102 78-79 0.007205807741385684 1102 80-81 0.0917105857628151 1102 82-83 0.20299427949363746 1102 84-85 0.10534935048562133 1102 86-87 -0.11146396763440691 1102 88-89 -0.14524189482134986 1102 90-91 -0.34980123091114024 1102 92-93 -0.2619752578930772 1102 94-95 -0.2927570718552346 1102 96-97 -0.3501625970881612 1102 98-99 -0.4494672829025035 1102 100-101 -0.3699577484508083 1103 1 0.2068992626559023 1103 2 0.38140057080407175 1103 3 0.35187121373355623 1103 4 0.3376051861397187 1103 5 0.25835184685248436 1103 6 0.19256405719036707 1103 7 0.31162404789761666 1103 8 5.381324633830786E-4 1103 9 0.3223752960591284 1103 10-11 0.38338115385605676 1103 12-13 0.3931080687399131 1103 14-15 0.45230581807934556 1103 16-17 0.5607209430886329 1103 18-19 0.348259537309481 1103 20-21 0.31540075482768515 1103 22-23 0.19508884212486777 1103 24-25 0.3246015084826652 1103 26-27 0.3360201066716755 1103 28-29 0.46807810164594343 1103 30-31 0.3211438541060261 1103 32-33 0.20747391601179999 1103 34-35 0.1825389884847155 1103 36-37 0.20842747584342902 1103 38-39 0.21265848488835104 1103 40-41 0.18255610459867455 1103 42-43 0.23979281371971695 1103 44-45 0.23220335641757828 1103 46-47 0.3713427787668735 1103 48-49 0.3230431060458656 1103 50-51 -0.09951238171725407 1103 52-53 0.11327591487728483 1103 54-55 0.2658086969835338 1103 56-57 0.30798333007571443 1103 58-59 0.20272640896086358 1103 60-61 0.45027350246190423 1103 62-63 0.21002802358143668 1103 64-65 0.22002410444171971 1103 66-67 0.13552630045473535 1103 68-69 0.23989166328890832 1103 70-71 0.421069625056802 1103 72-73 0.35817656889501137 1103 74-75 0.009935068811916636 1103 76-77 0.15869767009526115 1103 78-79 0.2921877743096033 1103 80-81 0.4991147945849157 1103 82-83 0.46113971721825564 1103 84-85 0.2399722051546398 1103 86-87 0.2501590955878683 1103 88-89 0.25243431785157355 1103 90-91 0.3626156937289835 1103 92-93 0.19793495054156907 1103 94-95 0.08742018852597155 1103 96-97 0.22511734364713476 1103 98-99 0.11575255792491035 1103 100-101 0.1277034404806514 1104 1 0.30089084405126343 1104 2 0.3654957599377475 1104 3 0.4343227681935069 1104 4 0.26552337088085665 1104 5 0.3801549865002727 1104 6 0.31667878837298247 1104 7 0.4527797355893455 1104 8 0.5256966782657386 1104 9 0.5945555584423303 1104 10-11 0.6650904371398667 1104 12-13 0.5957944986831691 1104 14-15 0.47342663171240673 1104 16-17 0.20380854506356627 1104 18-19 0.3836904927894196 1104 20-21 0.4527885311237796 1104 22-23 0.42930309873241157 1104 24-25 0.36797688653263805 1104 26-27 0.4275605806474161 1104 28-29 0.40950636112877703 1104 30-31 0.34647182082657935 1104 32-33 0.3414971611267248 1104 34-35 0.26979444575417233 1104 36-37 0.3954135790296718 1104 38-39 0.2781391478147768 1104 40-41 0.18011967336588697 1104 42-43 0.2632644577301946 1104 44-45 0.11490660441123879 1104 46-47 0.24536343497787527 1104 48-49 0.2709915225037278 1104 50-51 0.015702685212296785 1104 52-53 0.2220400915210945 1104 54-55 0.5220753673509719 1104 56-57 0.40987205092874035 1104 58-59 0.32491585777732723 1104 60-61 0.3246921351030707 1104 62-63 0.17446584211302962 1104 64-65 0.24024045968256047 1104 66-67 0.17217691460709972 1104 68-69 0.20983053420748377 1104 70-71 0.3256982364733716 1104 72-73 0.10437439259458259 1104 74-75 -0.2044244562543973 1104 76-77 0.02091412800800896 1104 78-79 0.20378131099061392 1104 80-81 0.32080096686590664 1104 82-83 0.24348425218700243 1104 84-85 0.4940253503904337 1104 86-87 0.2728249616757097 1104 88-89 0.304409757160812 1104 90-91 0.2429977093454312 1104 92-93 0.01321168201844003 1104 94-95 0.20915037708611095 1104 96-97 0.10869680297886575 1104 98-99 0.13460771218944245 1104 100-101 0.2449863145864768 1105 1 0.16007958515489662 1105 2 0.38034120776191926 1105 3 0.21106625663912482 1105 4 0.31744279514070683 1105 5 0.38788894317040246 1105 6 0.2850348620481995 1105 7 0.3863993458046977 1105 8 -0.019567679764577406 1105 9 0.46385597242359466 1105 10-11 0.3790829043238233 1105 12-13 0.1437084737248 1105 14-15 0.2880261303544529 1105 16-17 0.3304983254767535 1105 18-19 0.19982872967233334 1105 20-21 -0.04644418459829325 1105 22-23 -0.12971400071021577 1105 24-25 -0.06663548212228676 1105 26-27 -0.011424542912173763 1105 28-29 0.1239451713122719 1105 30-31 0.01555111703353873 1105 32-33 -0.004440009810444678 1105 34-35 -0.03676950974595172 1105 36-37 -0.16846419069916152 1105 38-39 -0.3153136376592869 1105 40-41 -0.3391449560051285 1105 42-43 -0.14808791426046497 1105 44-45 0.09883925217367562 1105 46-47 -0.08182017305679778 1105 48-49 -0.0431195628839518 1105 50-51 -0.027392067456922575 1105 52-53 0.060144602497949506 1105 54-55 0.05948254518368401 1105 56-57 0.27120936226604897 1105 58-59 0.16423051092176877 1105 60-61 0.251743557899168 1105 62-63 -0.020863737259112725 1105 64-65 0.1265711119706694 1105 66-67 -0.17553087884537177 1105 68-69 0.060282602744663905 1105 70-71 0.22080968530823242 1105 72-73 0.18169385768000623 1105 74-75 0.12792203087932208 1105 76-77 0.11219562780014769 1105 78-79 0.03170469582889268 1105 80-81 0.26165964815012543 1105 82-83 0.23529984506642876 1105 84-85 0.1438650975706075 1105 86-87 0.32107524981961433 1105 88-89 0.36722805348974674 1105 90-91 0.24655650439358467 1105 92-93 0.19462484428266436 1105 94-95 0.17888014415630593 1105 96-97 0.09730329424493434 1105 98-99 -0.08847136993219351 1105 100-101 0.016228992744046877 1106 1 0.18718092752444448 1106 2 0.22727995975011694 1106 3 0.1631347505266163 1106 4 0.3040536770167961 1106 5 0.43930469353730217 1106 6 0.43700269742928555 1106 7 0.6032085479899862 1106 8 0.6444419096870249 1106 9 0.6671969127738251 1106 10-11 0.5698699616376999 1106 12-13 0.572630313547144 1106 14-15 0.5188326278226256 1106 16-17 0.35089531186864065 1106 18-19 0.4297333346069081 1106 20-21 0.5163630735715614 1106 22-23 0.4810364814517101 1106 24-25 0.1965089052054978 1106 26-27 0.273807279006796 1106 28-29 0.1556103396271311 1106 30-31 0.18474020134847535 1106 32-33 0.16965713717402053 1106 34-35 0.09769411872426303 1106 36-37 0.30037335722888514 1106 38-39 0.38315093592515836 1106 40-41 0.3545683055609956 1106 42-43 0.43087637886324615 1106 44-45 0.17745508245267416 1106 46-47 0.2869180610395432 1106 48-49 0.2991174379535906 1106 50-51 0.4623449910095374 1106 52-53 0.4226276019677613 1106 54-55 0.6688115140871176 1106 56-57 0.6917763539598027 1106 58-59 0.6283104076507513 1106 60-61 0.5078940999951769 1106 62-63 0.5467046298447684 1106 64-65 0.46078808868089993 1106 66-67 0.4653752782279952 1106 68-69 0.37063160345925894 1106 70-71 0.48591595302912083 1106 72-73 0.6215755647253296 1106 74-75 0.4697996193781293 1106 76-77 0.4327222792527792 1106 78-79 0.5942249619976465 1106 80-81 0.5789698377108365 1106 82-83 0.6322228451509488 1106 84-85 0.659015163267572 1106 86-87 0.7961716822055287 1106 88-89 0.7722346616054345 1106 90-91 0.9413061259637026 1106 92-93 0.7898808319270252 1106 94-95 0.9221992239377776 1106 96-97 0.9477725857166241 1106 98-99 0.9652743175039333 1106 100-101 1.0437727330912026 1107 1 0.4116753298357487 1107 2 0.4608093621731726 1107 3 0.515036270731116 1107 4 0.4071781660372764 1107 5 0.37742044223478644 1107 6 0.49410917997275305 1107 7 0.5821781015512286 1107 8 0.6159938052078147 1107 9 0.6705254112851478 1107 10-11 0.685501979334795 1107 12-13 0.6738881466057975 1107 14-15 0.7178742949304251 1107 16-17 0.8318232036156843 1107 18-19 0.9255985912936069 1107 20-21 0.9756860161548175 1107 22-23 0.9023418128134679 1107 24-25 0.9296835628565745 1107 26-27 0.9395136717943231 1107 28-29 0.7194083011202963 1107 30-31 0.7542812785138864 1107 32-33 0.7603401204827094 1107 34-35 0.660574669799864 1107 36-37 0.7801479792363821 1107 38-39 0.8855399673491462 1107 40-41 0.8974520592650421 1107 42-43 0.8849035150370028 1107 44-45 0.858436821882826 1107 46-47 1.0549570348809212 1107 48-49 1.023297606243041 1107 50-51 0.9186330093559789 1107 52-53 1.0207765474236794 1107 54-55 1.1904017588044056 1107 56-57 1.1658347694321733 1107 58-59 1.120109296700413 1107 60-61 1.1939357865128457 1107 62-63 1.2479037333440743 1107 64-65 0.9480258789907836 1107 66-67 0.9076433420523529 1107 68-69 0.9841483130040842 1107 70-71 0.9668685482462962 1107 72-73 1.1050493108183623 1107 74-75 1.004210523960392 1107 76-77 1.121703678716127 1107 78-79 1.1907922971872686 1107 80-81 1.2648372765373956 1107 82-83 1.2659773579455802 1107 84-85 1.3873463968059419 1107 86-87 1.2311657504318063 1107 88-89 1.3695564084230973 1107 90-91 1.1597683231844442 1107 92-93 1.394044704748854 1107 94-95 1.445787067713031 1107 96-97 1.4062514195481413 1107 98-99 1.4810832885102982 1107 100-101 1.4945076907365156 1108 1 0.09976830896288291 1108 2 0.014765286247122589 1108 3 -0.035870774865308874 1108 4 0.057675882198452655 1108 5 0.3225653531990673 1108 6 0.18904054119013836 1108 7 0.4268262234878222 1108 8 0.16935126461482497 1108 9 0.29081171010644624 1108 10-11 0.14760269384567692 1108 12-13 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80-81 -3.1666894456245025 2316 82-83 -3.002934000481247 2316 84-85 -3.062749362634282 2316 86-87 -3.263041180440432 2316 88-89 -3.5203556738546204 2316 90-91 -3.3751190275213077 2316 92-93 -3.765545565488811 2316 94-95 -3.6212528345670982 2316 96-97 -4.053258171476106 2316 98-99 -4.771006830925103 2316 100-101 -5.260878966278806 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5914.0 3 2471.0 4 2439.0 5 2880.0 6 3074.0 7 3683.0 8 4445.0 9 5630.0 10 7300.0 11 8804.0 12 10809.0 13 12786.0 14 14091.0 15 16686.0 16 19747.0 17 23450.0 18 21458.0 19 16374.0 20 17142.0 21 18122.0 22 20725.0 23 23376.0 24 26756.0 25 31080.0 26 35969.0 27 42782.0 28 50389.0 29 60983.0 30 73804.0 31 90052.0 32 111143.0 33 140481.0 34 182430.0 35 244032.0 36 335031.0 37 426532.0 38 330659.0 39 50462.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.8616626151007 18.270904997662097 14.13481889861033 22.73261348862688 2 15.500962765321843 20.067301345946724 39.9430119918616 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46-47 23.009268624510142 26.651620931682114 27.989497790955436 22.34961265285231 48-49 22.856970443246055 26.651359253022804 27.870886850974447 22.620783452756687 50-51 22.153427607105517 27.019810724034198 28.244521051212367 22.582240617647916 52-53 22.845767517651925 27.260437736054833 27.323248695915765 22.570546050377477 54-55 22.29606092665469 26.737730655733007 27.745048566540742 23.22115985107156 56-57 22.798853224804024 26.885061849676216 27.395116181158404 22.920968744361357 58-59 23.307071238455116 26.61492035564295 27.352020600153494 22.725987805748442 60-61 22.777521496086308 26.88112933417616 28.157512398253324 22.18383677148421 62-63 22.43946730992152 26.975183872544438 28.03653834047786 22.54881047705618 64-65 22.236614974387763 27.3835420070772 27.611094960754635 22.768748057780407 66-67 22.467901308326674 27.060918246523887 27.231029984975635 23.240150460173805 68-69 22.26260630226896 26.95227665696807 27.71979026654562 23.065326774217347 70-71 22.749183005716905 26.813793474030717 27.47943905239691 22.95758446785547 72-73 22.72139844100853 26.570018284467828 27.94043112850452 22.76815214601912 74-75 22.199382217181515 27.630975110052713 27.37124328641854 22.798399386347228 76-77 22.499138909616985 26.956559744435594 27.319721355562766 23.224579990384658 78-79 22.576738348688323 27.065994669021908 27.412983544801616 22.944283437488156 80-81 22.856848945471565 27.109833903524837 27.1676250736677 22.865692077335893 82-83 22.728004508574774 26.974521379943056 27.60321173801267 22.6942623734695 84-85 22.539209622234274 27.189448263348314 27.3470586643115 22.92428345010591 86-87 22.469129762862362 27.32660578564824 27.30366914131138 22.900595310178016 88-89 22.377496474350878 27.26554784196591 26.90217641919156 23.454779264491652 90-91 22.54105978177508 26.86070541840947 27.225803786845432 23.372431012970026 92-93 22.910599609088337 26.94868342010011 27.397141067459934 22.743575903351623 94-95 22.485317905538977 26.76353547561325 27.551171800754975 23.1999748180928 96-97 22.020927020074943 26.80238421271059 28.059388789869704 23.117299977344764 98-99 22.12632989362856 27.171087630758546 27.148348322093685 23.55423415351921 100-101 21.950289583323723 26.799007693006576 27.455491493408385 23.795211230261312 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 154.0 1 140.5 2 172.5 3 252.0 4 261.0 5 253.0 6 401.0 7 825.5 8 1442.0 9 1846.0 10 1781.0 11 1667.0 12 1770.0 13 1924.0 14 2154.5 15 2367.0 16 2491.5 17 2673.0 18 2916.0 19 3207.5 20 3544.0 21 4087.5 22 4880.5 23 5872.5 24 7129.0 25 8824.5 26 11334.0 27 14377.5 28 17290.5 29 21386.0 30 26624.5 31 32134.5 32 38431.0 33 43803.0 34 48634.5 35 53034.5 36 58567.5 37 63993.0 38 66525.5 39 68373.0 40 70908.0 41 74304.5 42 77552.0 43 81152.5 44 84906.5 45 88914.5 46 91163.5 47 99675.0 48 119423.0 49 118466.0 50 99414.5 51 89389.5 52 84762.5 53 82826.0 54 80907.5 55 76140.0 56 69997.5 57 63361.5 58 57790.0 59 51495.5 60 43495.5 61 36306.0 62 29910.5 63 23482.5 64 17645.5 65 13357.5 66 9918.5 67 7684.5 68 5736.5 69 4071.5 70 3000.0 71 2257.0 72 1770.0 73 1535.5 74 1195.0 75 873.0 76 648.5 77 370.0 78 222.0 79 152.5 80 95.5 81 60.5 82 51.0 83 41.0 84 24.0 85 15.0 86 15.0 87 12.0 88 9.0 89 9.5 90 7.0 91 4.5 92 5.0 93 3.0 94 3.0 95 3.0 96 2.5 97 2.5 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013191580644061885 2 0.00629507039853409 3 0.01006409343969463 4 0.03484341513583519 5 0.043905108830540315 6 0.057497649372598 7 0.026463353267722926 8 0.024057593879748117 9 0.015396860083038795 10-11 0.044025396799939054 12-13 0.0416998293915634 14-15 0.019346315078297444 16-17 0.02235351431326596 18-19 0.0297512244312885 20-21 0.02421797783894644 22-23 0.01451474830744803 24-25 0.003327967153365156 26-27 0.002906959260469564 28-29 0.0015637436021836274 30-31 0.007117038189425485 32-33 0.0013833116480855168 34-35 0.0021651834491773305 36-37 0.00625497440873451 38-39 0.006355214383233461 40-41 0.004149934944256551 42-43 7.217278163924435E-4 44-45 0.0011026397194884552 46-47 0.0025460953522733424 48-49 6.615838316930732E-4 50-51 0.0015035996174842573 52-53 9.422557602901346E-4 54-55 0.0026262873318725027 56-57 0.0026062393369727125 58-59 0.0021250874593777506 60-61 5.813918520939128E-4 62-63 0.0012830716735865663 64-65 0.0036086390819622177 66-67 0.002646335326772293 68-69 0.007337566133323176 70-71 0.010845965240786442 72-73 0.0038091190309601187 74-75 0.003869263015659489 76-77 0.0033680631431647363 78-79 0.03414173531434254 80-81 0.022233226343867218 82-83 0.0040296469748578095 84-85 0.008319917883412891 86-87 0.007578142072120657 88-89 0.005934206490337868 90-91 0.01216913290417259 92-93 0.01431426835845013 94-95 0.006455454357732411 96-97 0.004410558877953821 98-99 0.021471402537675193 100-101 0.04155949342726487 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2494015.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.012028152555445 #Duplication Level Percentage of deduplicated Percentage of total 1 80.1080002111603 48.0744356391707 2 12.043334782501262 14.45489892036232 3 3.4875224382796195 6.27879884246116 4 1.4775900125726071 3.546926937297682 5 0.7488652877453528 2.247046236532282 6 0.4475056210611809 1.611343195773024 7 0.3059630315831386 1.2853023445505969 8 0.21757814450478458 1.0445844586721529 9 0.15766849362055224 0.851580547013483 >10 0.8944515351334078 9.86511683270429 >50 0.0734097988413392 3.0229945418257334 >100 0.0346275704436541 3.750618051346468 >500 0.0018755006053417557 0.717161017741069 >1k 0.0012726611250468533 1.6723305768151266 >5k 2.679286579046007E-4 1.1006437882790898 >10k+ 6.698216447615018E-5 0.47621806945481826 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11847 0.4750171911556266 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7862 0.3152346718042995 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7777 0.3118265126713352 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6399 0.25657423872751367 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5343 0.21423287349915698 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4901 0.19651044600774253 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3641 0.1459894988602715 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3583 0.14366393145189585 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3275 0.13131436659362514 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2785 0.11166733159183083 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2757 0.1105446438774426 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.00959897995802E-5 0.0 0.0 0.0 0.0 6 4.00959897995802E-5 0.0 0.0 0.0 0.0 7 4.00959897995802E-5 0.0 0.0 0.0 0.0 8 4.00959897995802E-5 0.0 0.0 0.0 0.0 9 4.00959897995802E-5 0.0 0.0 4.00959897995802E-5 0.0 10-11 4.00959897995802E-5 0.0 0.0 4.00959897995802E-5 0.0 12-13 4.00959897995802E-5 0.0 0.0 4.00959897995802E-5 0.0 14-15 8.01919795991604E-5 0.0 0.0 4.00959897995802E-5 0.0 16-17 8.01919795991604E-5 0.0 0.0 4.00959897995802E-5 0.0 18-19 8.01919795991604E-5 0.0 0.0 4.00959897995802E-5 0.0 20-21 8.01919795991604E-5 0.0 0.0 4.00959897995802E-5 4.00959897995802E-5 22-23 8.01919795991604E-5 4.00959897995802E-5 0.0 6.01439846993703E-5 4.00959897995802E-5 24-25 8.01919795991604E-5 4.00959897995802E-5 0.0 2.2052794389769106E-4 4.00959897995802E-5 26-27 8.01919795991604E-5 4.00959897995802E-5 0.0 3.8091190309601187E-4 4.00959897995802E-5 28-29 1.2028796939874058E-4 4.00959897995802E-5 0.0 6.214878418934931E-4 4.00959897995802E-5 30-31 1.2028796939874058E-4 4.00959897995802E-5 0.0 0.0012429756837869861 4.00959897995802E-5 32-33 1.2028796939874058E-4 4.00959897995802E-5 0.0 0.0019446555052796393 4.00959897995802E-5 34-35 1.4033596429853068E-4 4.00959897995802E-5 0.0 0.0027265273063714532 4.00959897995802E-5 36-37 1.603839591983208E-4 4.00959897995802E-5 0.0 0.0040496949697576 4.00959897995802E-5 38-39 1.603839591983208E-4 4.00959897995802E-5 0.0 0.005152334689246055 4.00959897995802E-5 40-41 1.603839591983208E-4 4.00959897995802E-5 0.0 0.006776222276129053 4.00959897995802E-5 42-43 1.804319540981109E-4 6.01439846993703E-5 0.0 0.009262173643703025 4.00959897995802E-5 44-45 2.4057593879748117E-4 8.01919795991604E-5 0.0 0.013652684526757056 4.00959897995802E-5 46-47 3.0071992349685144E-4 8.01919795991604E-5 0.0 0.024779321696140562 4.00959897995802E-5 48-49 3.207679183966416E-4 8.01919795991604E-5 0.0 0.045468852432723944 4.00959897995802E-5 50-51 3.207679183966416E-4 8.01919795991604E-5 0.0 0.0615473443423556 4.00959897995802E-5 52-53 3.207679183966416E-4 8.01919795991604E-5 0.0 0.07728502033869084 4.00959897995802E-5 54-55 3.207679183966416E-4 8.01919795991604E-5 0.0 0.10050059843264775 8.01919795991604E-5 56-57 3.207679183966416E-4 8.01919795991604E-5 0.0 0.1188445137659557 8.01919795991604E-5 58-59 3.207679183966416E-4 8.01919795991604E-5 0.0 0.14316273157940107 8.01919795991604E-5 60-61 3.207679183966416E-4 8.01919795991604E-5 0.0 0.18059233805730918 8.01919795991604E-5 62-63 3.207679183966416E-4 1.0023997449895049E-4 0.0 0.20771727515672522 8.01919795991604E-5 64-65 3.207679183966416E-4 1.2028796939874058E-4 0.0 0.2397539710065898 8.01919795991604E-5 66-67 3.207679183966416E-4 1.2028796939874058E-4 0.0 0.30182256321633993 8.01919795991604E-5 68-69 3.4081591329643165E-4 1.2028796939874058E-4 0.0 0.3703065137940229 8.01919795991604E-5 70-71 3.6086390819622174E-4 1.603839591983208E-4 0.0 0.4265611874828339 8.01919795991604E-5 72-73 3.6086390819622174E-4 1.603839591983208E-4 0.0 0.49163697892755254 8.01919795991604E-5 74-75 3.6086390819622174E-4 1.603839591983208E-4 0.0 0.5494553962185472 8.01919795991604E-5 76-77 3.8091190309601187E-4 2.0047994899790098E-4 0.0 0.598091831845438 8.01919795991604E-5 78-79 4.0095989799580195E-4 2.0047994899790098E-4 0.0 0.6666158784129206 8.01919795991604E-5 80-81 4.8115187759496233E-4 2.4057593879748117E-4 0.0 0.774113227065595 8.01919795991604E-5 82-83 4.8115187759496233E-4 2.8067192859706136E-4 0.0 0.87966592021299 1.0023997449895049E-4 84-85 5.011998724947525E-4 2.8067192859706136E-4 0.0 1.0087148633829388 1.2028796939874058E-4 86-87 5.212478673945425E-4 2.8067192859706136E-4 0.0 1.2366204694037526 1.2028796939874058E-4 88-89 5.212478673945425E-4 2.8067192859706136E-4 0.0 1.5244294841851391 1.2028796939874058E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11170 0.0 26.421888 1 TACATGG 11380 0.0 25.641466 2 GTACCTG 2360 0.0 24.36682 1 ACATGGG 11575 0.0 23.936699 3 TACCTGG 2405 0.0 23.317589 2 GAGTACT 8100 0.0 21.997187 12-13 GTGTAGC 1350 0.0 20.770344 1 GTACTTT 8595 0.0 20.729916 14-15 CATGGGA 6570 0.0 20.470942 4 AGAGTAC 14210 0.0 19.211075 10-11 AGTACTT 8585 0.0 19.149467 12-13 CATGGGG 5200 0.0 18.552797 4 ACTTTTT 10335 0.0 17.699024 16-17 GTGTACG 245 3.7461523E-6 17.458296 1 GTATCAA 24255 0.0 17.223167 1 ATGGGAG 2830 0.0 17.128595 5 TACTTTT 9530 0.0 17.125608 14-15 GAGTACA 8830 0.0 16.846476 1 GAGTACG 455 9.094947E-12 16.712215 1 GTACACG 495 1.8189894E-12 16.321842 1 >>END_MODULE