FastQCFastQC Report
Thu 26 May 2016
SRR936586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936586_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494015
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT142530.5714881426134165No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82560.3310324917853341No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73270.2937833172615241No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT60160.24121747463427445No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48350.19386411068097023No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43390.1739764997403785No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42530.17052824461761457No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40040.1605443431575191No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC36810.1475933384522547No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC36380.14586921089087274No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT28650.11487501077579725No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG27940.11202819550002706No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA26150.10485101332590221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG22150.024.922051
TACCTGG21800.023.9700762
GTACATG109150.022.516761
GAGTACT96300.022.14140912-13
TACATGG109600.021.7583712
ACATGGG109600.020.6727143
GTACTTT102150.020.59446514-15
AGTACTT99400.019.03825412-13
AGAGTAC161100.018.62973610-11
CGTATAC2302.0600019E-618.5867883
ACTTTTT115550.018.26874716-17
GTATAGA8400.017.7150381
TAAGGTG11950.017.4858765
ATTCGCG3450.017.20912680-81
GTATTAG7850.017.1217461
CATGGGG52350.016.8756224
TAGCCCT14050.016.564694
TACTTTT111950.016.56464414-15
GAGTACA91800.016.4712891
GTAAGGT11050.016.3337124
Produced by FastQC (version 0.11.5)