##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936586_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2494015 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.46489134989164 31.0 31.0 34.0 30.0 34.0 2 31.680533196472354 31.0 31.0 34.0 30.0 34.0 3 31.95826368325772 31.0 31.0 34.0 30.0 34.0 4 35.76645288821439 37.0 35.0 37.0 35.0 37.0 5 35.57481530784698 37.0 35.0 37.0 33.0 37.0 6 35.6774822926085 37.0 35.0 37.0 33.0 37.0 7 35.610829926844865 37.0 35.0 37.0 33.0 37.0 8 35.58866005216488 37.0 35.0 37.0 33.0 37.0 9 37.2120829265261 39.0 37.0 39.0 33.0 39.0 10-11 37.094976172958056 39.0 37.0 39.0 33.0 39.0 12-13 36.972420374376256 39.0 37.0 39.0 33.0 39.0 14-15 38.07373712668127 40.0 37.0 41.0 33.0 41.0 16-17 37.99216083303428 40.0 37.0 41.0 33.0 41.0 18-19 38.04787741853998 40.0 37.0 41.0 33.0 41.0 20-21 38.12577590752261 40.0 37.0 41.0 33.0 41.0 22-23 38.03853946347556 40.0 37.0 41.0 33.0 41.0 24-25 37.932151771340585 40.0 37.0 41.0 33.0 41.0 26-27 37.728490005072146 39.5 37.0 41.0 32.5 41.0 28-29 37.65968668993571 39.5 37.0 41.0 32.5 41.0 30-31 37.67573370649335 40.0 37.0 41.0 33.0 41.0 32-33 37.5178962436072 39.5 36.5 41.0 32.0 41.0 34-35 37.348958807384875 39.0 36.0 41.0 32.0 41.0 36-37 37.1257877358396 39.0 36.0 41.0 31.0 41.0 38-39 36.98352295395176 39.0 36.0 41.0 31.0 41.0 40-41 36.77813585724223 39.0 35.0 41.0 30.5 41.0 42-43 36.54997684456589 39.0 35.0 40.0 30.0 41.0 44-45 36.29067988765104 39.0 35.0 40.0 30.0 41.0 46-47 35.92361573607216 38.5 35.0 40.0 29.5 41.0 48-49 35.49992321617954 38.0 35.0 40.0 27.5 41.0 50-51 35.30645886251686 38.0 34.0 40.0 27.0 41.0 52-53 35.04506488533549 38.0 34.0 40.0 26.5 41.0 54-55 34.77702459688494 38.0 34.0 40.0 26.0 41.0 56-57 34.48247103565937 38.0 33.5 40.0 26.0 41.0 58-59 34.11763762447299 37.0 33.0 40.0 24.5 41.0 60-61 33.72472659546955 37.0 33.0 39.5 23.0 41.0 62-63 33.44645741906123 36.0 32.0 39.0 23.0 40.5 64-65 33.00541476294248 36.0 32.0 39.0 22.0 40.0 66-67 32.58525129159207 35.0 31.0 38.5 22.0 40.0 68-69 32.11280665914199 35.0 31.0 38.0 20.5 40.0 70-71 31.62480919320854 35.0 30.5 37.5 19.5 39.5 72-73 31.197904383093125 34.0 30.0 37.0 18.0 39.0 74-75 30.826902003396132 34.0 30.0 36.0 18.0 39.0 76-77 29.398045120017322 32.5 28.5 35.0 15.0 37.0 78-79 30.219359145795032 34.0 29.5 35.0 16.5 37.0 80-81 30.051918292391985 34.0 30.0 35.0 12.5 37.0 82-83 29.70457775915542 34.0 29.0 35.0 8.5 36.5 84-85 29.245581923123957 34.0 29.0 35.0 7.0 36.0 86-87 28.870788467591414 34.0 29.0 35.0 2.0 36.0 88-89 28.449451988059415 33.0 28.5 35.0 2.0 35.0 90-91 27.979719448359372 33.0 27.0 35.0 2.0 35.0 92-93 27.507123654027744 33.0 26.5 35.0 2.0 35.0 94-95 26.939141504762404 32.5 25.5 34.0 2.0 35.0 96-97 26.227341254964387 32.0 25.0 34.0 2.0 35.0 98-99 25.337482132224544 32.0 22.0 34.0 2.0 35.0 100-101 22.495110494523892 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3909971356726025 1101 2 0.45745873064637266 1101 3 0.24589479352575339 1101 4 0.08492930955384281 1101 5 0.22711165268398759 1101 6 0.1366710641859541 1101 7 0.1996251266591571 1101 8 0.156750147421036 1101 9 0.21518331314126726 1101 10-11 0.2901187039154607 1101 12-13 0.22949207418511008 1101 14-15 0.36500616274688724 1101 16-17 0.31071888002988857 1101 18-19 0.31204654969473467 1101 20-21 0.11671039325170085 1101 22-23 0.008956617011619983 1101 24-25 0.004178871007162854 1101 26-27 0.10923962745959415 1101 28-29 0.08511499038232273 1101 30-31 0.018697959740720194 1101 32-33 0.06927332100327277 1101 34-35 0.11810671988407506 1101 36-37 0.04741055020512164 1101 38-39 0.21672211811077347 1101 40-41 0.18566243631456558 1101 42-43 0.31619731369035975 1101 44-45 0.2978976321136102 1101 46-47 0.37577641183386135 1101 48-49 0.2768607400797194 1101 50-51 0.3749059020582308 1101 52-53 0.3072720073034674 1101 54-55 0.23993650306851322 1101 56-57 0.27168219397131566 1101 58-59 0.2101982111971381 1101 60-61 0.28295729268482717 1101 62-63 0.24747983112792582 1101 64-65 0.28165969858216044 1101 66-67 0.04674012881569922 1101 68-69 -0.11401031516808757 1101 70-71 -0.09169813620178502 1101 72-73 -0.047730415977692786 1101 74-75 -0.055390491390408414 1101 76-77 0.19909745785728106 1101 78-79 0.30700509757981465 1101 80-81 0.11946082212686449 1101 82-83 0.16613830227418092 1101 84-85 0.3628719457469849 1101 86-87 0.18461676520593784 1101 88-89 0.00436673847653779 1101 90-91 0.09937667824408791 1101 92-93 0.22437290167970403 1101 94-95 -0.015406629365177338 1101 96-97 0.128086518301874 1101 98-99 -0.025718041236981293 1101 100-101 -0.041885311919202195 1102 1 0.40173974273182367 1102 2 0.41246193773343265 1102 3 0.18764430994140113 1102 4 0.12933183326914133 1102 5 0.13499702756019616 1102 6 0.20089310857284914 1102 7 0.12729232884884567 1102 8 -0.01937944310051165 1102 9 0.020631377492783542 1102 10-11 0.14633593968880376 1102 12-13 0.13657063470728303 1102 14-15 0.18489450216159042 1102 16-17 0.3341067893116829 1102 18-19 0.20369818944406148 1102 20-21 0.24929236811136235 1102 22-23 0.07340400212629561 1102 24-25 0.10087796656827663 1102 26-27 -0.15804876530570056 1102 28-29 -0.06533811151631141 1102 30-31 0.051014908691556116 1102 32-33 0.1999375359628246 1102 34-35 0.23407304647849259 1102 36-37 0.12030484037993716 1102 38-39 0.1183385454561261 1102 40-41 0.34288300876951183 1102 42-43 0.1465620744598155 1102 44-45 0.028354445358274916 1102 46-47 0.08231956278681452 1102 48-49 0.26072533545223564 1102 50-51 0.037271870023666054 1102 52-53 0.04589138658084124 1102 54-55 0.11639847589097485 1102 56-57 0.1964690058847367 1102 58-59 0.23554913762171736 1102 60-61 0.2411303977300463 1102 62-63 0.1826558275940826 1102 64-65 0.15413134410020035 1102 66-67 0.13764150685461374 1102 68-69 0.09553084446618243 1102 70-71 0.16257375670571506 1102 72-73 0.0407259994066429 1102 74-75 -0.39140919284287534 1102 76-77 -0.0011415104692034106 1102 78-79 0.1639335275997631 1102 80-81 0.19256557738981783 1102 82-83 0.1874254056492859 1102 84-85 0.12977905569762882 1102 86-87 0.048989885580621007 1102 88-89 0.08921537001853963 1102 90-91 -0.05033081373315085 1102 92-93 0.025935020333854197 1102 94-95 -0.137590406042996 1102 96-97 0.05413555345275611 1102 98-99 -0.3180118799991689 1102 100-101 -0.47176362666554894 1103 1 0.45816698828697255 1103 2 0.4146636077409056 1103 3 0.2829946596387565 1103 4 0.06699022501715035 1103 5 0.04351257317899382 1103 6 0.0067920521666025024 1103 7 0.033535039369027686 1103 8 0.17322294567438234 1103 9 -0.021293262629306753 1103 10-11 0.09837635157654034 1103 12-13 0.16237712298678986 1103 14-15 0.14805606569817797 1103 16-17 0.138879446497576 1103 18-19 0.24276139985946799 1103 20-21 0.12058647069979145 1103 22-23 -0.004623207648620564 1103 24-25 0.05012860804249186 1103 26-27 0.052096559740853365 1103 28-29 0.18937534026736813 1103 30-31 0.2041167182616732 1103 32-33 0.08235872062437721 1103 34-35 0.12094254353898037 1103 36-37 0.18446091135525222 1103 38-39 0.3310196210769405 1103 40-41 0.33216883248567797 1103 42-43 0.25074776790432196 1103 44-45 0.29883738945810023 1103 46-47 0.2861414494129164 1103 48-49 0.43253985747980295 1103 50-51 0.266954977803195 1103 52-53 0.2788503124624029 1103 54-55 0.36846646425153295 1103 56-57 0.3109413908641514 1103 58-59 0.3638557197996093 1103 60-61 0.3361992993759344 1103 62-63 0.2159255077069986 1103 64-65 0.30040575273805814 1103 66-67 0.20997365607990304 1103 68-69 0.18122176854489425 1103 70-71 0.3319692833866874 1103 72-73 0.10056286826123895 1103 74-75 0.30226450236494884 1103 76-77 0.3652425714813532 1103 78-79 0.4678982880303941 1103 80-81 0.5575109137041778 1103 82-83 0.5735769507224902 1103 84-85 0.6903196682313855 1103 86-87 0.6442836491464696 1103 88-89 0.6261144697801377 1103 90-91 0.40519755247450107 1103 92-93 0.6128451408005411 1103 94-95 0.5133311077571001 1103 96-97 0.39974659471664964 1103 98-99 0.22431874708389543 1103 100-101 0.28788875657427937 1104 1 0.28653837858978193 1104 2 0.3492409740887368 1104 3 0.15490262042004943 1104 4 0.021872679199816503 1104 5 -0.04719731459177723 1104 6 0.13951402918529254 1104 7 -0.0013127372562209416 1104 8 0.008873110575770227 1104 9 0.044918569152855525 1104 10-11 -0.006513434558875986 1104 12-13 -0.08992808225753635 1104 14-15 0.14414022570815632 1104 16-17 7.601943125408184E-4 1104 18-19 0.06265578780843839 1104 20-21 0.03953179447367461 1104 22-23 0.044398817930428436 1104 24-25 -0.034732481542171456 1104 26-27 0.08276610580578136 1104 28-29 0.04353285222753556 1104 30-31 -0.04061502192142541 1104 32-33 0.016787114464939634 1104 34-35 0.14923817033005804 1104 36-37 0.22823187097645814 1104 38-39 0.2054179895270991 1104 40-41 0.2685713883773886 1104 42-43 0.24621196648259058 1104 44-45 0.3341794895307828 1104 46-47 0.2959085438546012 1104 48-49 0.3101391698236924 1104 50-51 0.3466433563019322 1104 52-53 0.15419581691917728 1104 54-55 0.3615381373441977 1104 56-57 0.4292396851596507 1104 58-59 0.44483179552726426 1104 60-61 0.2935916885387897 1104 62-63 0.01339185748265237 1104 64-65 0.06021024382316398 1104 66-67 0.07313614467975782 1104 68-69 0.20913857777730982 1104 70-71 -0.03234279890999758 1104 72-73 -0.035350166182237075 1104 74-75 0.05997496563874094 1104 76-77 0.21199180224038017 1104 78-79 0.2896919833370326 1104 80-81 0.3234041187156471 1104 82-83 0.26682653113092414 1104 84-85 0.40793908756528907 1104 86-87 0.2993873637323361 1104 88-89 0.2848950911219923 1104 90-91 0.2962523490576423 1104 92-93 0.2620788105030698 1104 94-95 0.2362812988668459 1104 96-97 0.11740820041184463 1104 98-99 0.42429548624888014 1104 100-101 0.2117754112167063 1105 1 0.3226722466385432 1105 2 0.35848609929131214 1105 3 0.11091032749159169 1105 4 0.0877342854869525 1105 5 -0.08077560136155171 1105 6 0.033338414499041846 1105 7 0.10060793168572957 1105 8 0.00285880668912597 1105 9 0.09037834227220287 1105 10-11 0.03225822602129824 1105 12-13 -0.025036830132243892 1105 14-15 0.16801521979591172 1105 16-17 0.05384924633776933 1105 18-19 0.02770648371019746 1105 20-21 0.0761758577134799 1105 22-23 -0.002604983328687638 1105 24-25 -0.049919905772149775 1105 26-27 0.05015985351867158 1105 28-29 0.05272445609998755 1105 30-31 0.05154982875406233 1105 32-33 0.02026600092255393 1105 34-35 0.06486851468380195 1105 36-37 0.11918240979934325 1105 38-39 -0.055776432305627566 1105 40-41 0.005723344146367992 1105 42-43 0.10106081228888542 1105 44-45 -0.12459813307663126 1105 46-47 0.0816065864675366 1105 48-49 0.23483129770730926 1105 50-51 0.24186527067278263 1105 52-53 0.0909328579115325 1105 54-55 0.2077661282530343 1105 56-57 0.1989520266805087 1105 58-59 0.20081915204653455 1105 60-61 0.17257498241569635 1105 62-63 0.042080037234661916 1105 64-65 0.13066398023434544 1105 66-67 0.16784195161961435 1105 68-69 -0.012145859464581577 1105 70-71 0.02211291405635407 1105 72-73 -0.09154575801612452 1105 74-75 -0.07304084929196719 1105 76-77 -0.11132302267018801 1105 78-79 0.06933757255709239 1105 80-81 0.024655522094708004 1105 82-83 0.03345031849939062 1105 84-85 0.14009955695980025 1105 86-87 -0.09998985958105777 1105 88-89 -0.013546380191822749 1105 90-91 -0.3246734330383525 1105 92-93 -0.019273237870255144 1105 94-95 0.3043489797004817 1105 96-97 0.33918634080700727 1105 98-99 0.18321873666149102 1105 100-101 0.23778304360731894 1106 1 0.36802927698208876 1106 2 0.38362037466531973 1106 3 0.17083441099972774 1106 4 0.08388160036366799 1106 5 -0.10706283924603355 1106 6 -0.05548003904821286 1106 7 0.013871418773007349 1106 8 0.09627143741072075 1106 9 0.025363704527570974 1106 10-11 0.014232093228905285 1106 12-13 0.08444043718140648 1106 14-15 0.15446013039426276 1106 16-17 0.20094935929044055 1106 18-19 0.09524488377781637 1106 20-21 0.08664703172975408 1106 22-23 0.13157011636933902 1106 24-25 -0.022880610535374046 1106 26-27 0.05246174451550445 1106 28-29 0.12621707575682706 1106 30-31 0.017489986887810005 1106 32-33 0.14945466262545182 1106 34-35 -0.15869441225041925 1106 36-37 0.05074269348728677 1106 38-39 0.10000259537930845 1106 40-41 0.217152118648265 1106 42-43 0.05446341557911438 1106 44-45 0.06384995863786713 1106 46-47 0.0810354839111227 1106 48-49 0.05600838752389592 1106 50-51 0.03339151488107461 1106 52-53 0.26992135517992466 1106 54-55 0.03241439976975613 1106 56-57 -0.08955081250407915 1106 58-59 -0.210704902262961 1106 60-61 0.0236464960583902 1106 62-63 -0.42775391464903834 1106 64-65 -0.15263439155668124 1106 66-67 -0.06532252899017266 1106 68-69 -0.06216276394656717 1106 70-71 -0.11953089314034315 1106 72-73 0.02292774420834931 1106 74-75 -0.016068518855448133 1106 76-77 0.028702372472078963 1106 78-79 0.10069116053339044 1106 80-81 0.0568547183634287 1106 82-83 0.11886232682869036 1106 84-85 0.25208675565661665 1106 86-87 0.14567817481188072 1106 88-89 0.005516304700950769 1106 90-91 -0.28178349658383794 1106 92-93 0.26363517825662086 1106 94-95 0.1422173245211802 1106 96-97 0.24068373213808414 1106 98-99 0.34250429178162634 1106 100-101 0.4288190578378206 1107 1 0.5773353094518612 1107 2 0.6012627989933037 1107 3 0.4276491867333938 1107 4 0.22872407456912924 1107 5 0.08124994903464255 1107 6 0.16378693252985954 1107 7 0.14649699768706625 1107 8 0.24743858814776587 1107 9 0.22550724933437039 1107 10-11 0.31439778293215426 1107 12-13 0.3495990305187675 1107 14-15 0.42527721531420326 1107 16-17 0.44978682574960516 1107 18-19 0.35199062213512633 1107 20-21 0.33320597068041735 1107 22-23 0.40653892823829096 1107 24-25 0.41168144105252935 1107 26-27 0.4216798826267194 1107 28-29 0.3834981294892259 1107 30-31 0.41221151702075076 1107 32-33 0.3745669457789731 1107 34-35 0.3405147826164381 1107 36-37 0.41909231346556197 1107 38-39 0.4508713487833873 1107 40-41 0.5916229791407019 1107 42-43 0.4097984240429966 1107 44-45 0.38853773912518363 1107 46-47 0.5909384875001891 1107 48-49 0.5269452695695378 1107 50-51 0.4059090366995335 1107 52-53 0.2976684251719135 1107 54-55 0.5196608657030808 1107 56-57 0.589479205741192 1107 58-59 0.38752734388241805 1107 60-61 0.2848098912797923 1107 62-63 0.28419891819199705 1107 64-65 0.36100854095697343 1107 66-67 0.2504311238884256 1107 68-69 0.2704846069572113 1107 70-71 0.07892009635127195 1107 72-73 0.29166282654736975 1107 74-75 0.5401978251368433 1107 76-77 0.3673553334182422 1107 78-79 0.47358393982670677 1107 80-81 0.36166201365106687 1107 82-83 0.2764297349523659 1107 84-85 0.3456800430179712 1107 86-87 0.6387631147530364 1107 88-89 0.7275724892146975 1107 90-91 0.5293731051832822 1107 92-93 0.5693246668124416 1107 94-95 0.6492978096032225 1107 96-97 0.7555827184723576 1107 98-99 0.8478013480690691 1107 100-101 0.7054393260939769 1108 1 0.36897071111699375 1108 2 0.36689782362415 1108 3 0.05687907875324427 1108 4 0.03543234892647007 1108 5 0.0051638121526522696 1108 6 -0.018345814443051722 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-1.315476633029533 2316 62-63 -1.3697649194241208 2316 64-65 -1.4007140733511498 2316 66-67 -1.5402500499564304 2316 68-69 -1.6059992873605573 2316 70-71 -1.2451004429176393 2316 72-73 -1.1892998951319633 2316 74-75 -1.2000995569357826 2316 76-77 -1.3149676247190456 2316 78-79 -1.2796281484830985 2316 80-81 -1.3747288356673089 2316 82-83 -1.1337531881987957 2316 84-85 -1.3135942964680964 2316 86-87 -1.3078996310546565 2316 88-89 -1.1719585157316175 2316 90-91 -1.2656949066396166 2316 92-93 -1.3959576350333762 2316 94-95 -1.630151611316066 2316 96-97 -1.6253920673884217 2316 98-99 -2.118086424883032 2316 100-101 -2.6566663259750314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 18.0 4 26.0 5 81.0 6 184.0 7 409.0 8 999.0 9 1832.0 10 3118.0 11 4838.0 12 6897.0 13 9186.0 14 11924.0 15 15384.0 16 19328.0 17 23920.0 18 21249.0 19 15273.0 20 14529.0 21 15397.0 22 16531.0 23 18777.0 24 22518.0 25 27046.0 26 33443.0 27 41555.0 28 51784.0 29 63507.0 30 78781.0 31 97305.0 32 121062.0 33 152313.0 34 198402.0 35 264352.0 36 369967.0 37 472267.0 38 283785.0 39 16028.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.34006518484908 19.346206902139677 14.804739169450224 22.50898874356102 2 15.73216324412633 21.142288627440287 38.90239173473451 24.223156393698876 3 20.427257140199238 24.42042769821501 29.672385669399194 25.47992949218656 4 13.254305418656 16.995105300599345 36.86363093180544 32.88695834893922 5 13.641778417531569 37.808393293544746 33.832354657048974 14.717473631874709 6 31.946920928703314 36.32949280577703 16.959320613548837 14.764265651970817 7 28.826971770418382 32.76279412914517 20.207897707110824 18.20233639332562 8 26.762068391729798 34.150797008037244 19.206179593947912 19.88095500628505 9 26.124193481772917 17.14852927083254 20.46939350791672 36.25788373947782 10-11 25.43208544957135 26.500786484732014 26.714378336244977 21.35274972945166 12-13 26.763797884896018 23.860566969438228 27.25910374618913 22.116531399476628 14-15 23.03600201041414 25.505455363413233 26.29987768710955 25.15866493906308 16-17 22.18348726852084 28.656042565902773 26.790596688472203 22.36987347710419 18-19 21.988660854084678 27.465833204692032 29.000787886199564 21.544718055023726 20-21 23.083742479495914 25.981299230357475 28.998682846735086 21.936275443411528 22-23 22.51171522431843 26.099518961081035 29.119775362804067 22.26899045179647 24-25 22.872977909114418 26.238013805049288 29.10146891658631 21.787539369249984 26-27 22.512889364873125 26.144224961091833 29.495730476198307 21.84715519783674 28-29 21.945938576953225 27.118882604956262 29.10403505993348 21.83114375815703 30-31 22.324569484259214 26.244153752915228 29.052377201495823 22.378899561329735 32-33 22.251511224996946 26.439936637916084 29.113558351198442 22.194993785888524 34-35 23.017773295009274 26.308017118722592 28.893447840402214 21.78076174586592 36-37 22.777919787967093 26.025214995073082 29.169059956033642 22.02780526092618 38-39 22.347105787782898 26.603560848945396 29.16283081236177 21.88650255090994 40-41 22.651743295399392 26.57008056126439 28.769351153325427 22.00882499001079 42-43 22.32067543542539 26.71872904126514 28.293372262820554 22.667223260488917 44-45 22.741672041310952 26.297738519429014 28.347568037784033 22.613021401476004 46-47 23.182727741313077 26.906036553447333 27.90619112661788 22.005044578621707 48-49 23.160268246240058 26.86723046203243 27.76381065730835 22.20869063441916 50-51 22.410422264251864 27.176166706829612 28.10998525665726 22.303425772261264 52-53 23.083384824257898 27.457600681965722 27.22816701263489 22.230847481141492 54-55 22.60760875863586 26.931808771909072 27.589714984835055 22.870867484620018 56-57 23.041866854644883 27.046167738479316 27.378156995492215 22.53380841138359 58-59 23.65411329728785 26.768314161489364 27.18583981377546 22.39173272744733 60-61 22.934702128798538 27.061077525445874 28.07973466154932 21.924485684206264 62-63 22.67307021448076 27.10226429771485 27.91602009679502 22.30864539100937 64-65 22.429670606428097 27.534938112379447 27.52026025525034 22.515131025942107 66-67 22.770306113897643 27.131395650498547 27.143765630381793 22.954532605222017 68-69 22.606783939746787 27.085638859217774 27.509033651856768 22.79854354917867 70-71 23.06981675117663 27.02529204253753 27.31468821396752 22.59020299231832 72-73 22.99322578252336 26.753066842019795 27.731689665058152 22.522017710398696 74-75 22.520338193703367 27.877263744857938 27.114297049997106 22.488101011441593 76-77 22.80066119148791 27.1698778960959 27.12727535177171 22.902185560644472 78-79 22.87728027531526 27.235021692465445 27.195863373758833 22.691834658460465 80-81 23.18229859166 27.314000046919933 26.99494447707229 22.50875688434777 82-83 23.03120967360331 27.19666329017682 27.339783150754254 22.432343885465613 84-85 22.722225675223203 27.4664819422529 27.123592261624307 22.68770012089959 86-87 22.763279251245116 27.503248133325847 26.997521794573615 22.73595082085542 88-89 22.680188057196855 27.53344584892856 26.614938370438345 23.17142772343624 90-91 22.85001716537151 27.058480976918194 26.975441486938273 23.116060370772022 92-93 23.168117814971424 27.24751894037738 27.062450668008047 22.521912576643143 94-95 22.813308944815667 27.043686202943984 27.2312917272921 22.911713124948246 96-97 22.367588966091372 27.073750135840633 27.68905306947702 22.869607828590972 98-99 22.440491383308295 27.451888943314156 26.73441108730256 23.373208586074988 100-101 22.26581156172142 27.259036567325477 26.95100389817819 23.52414797277491 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 320.0 1 237.0 2 221.0 3 314.5 4 291.5 5 276.5 6 448.5 7 846.5 8 1352.5 9 1768.0 10 1819.5 11 1723.5 12 1821.0 13 2010.0 14 2269.0 15 2566.0 16 2826.5 17 3078.5 18 3368.5 19 3660.0 20 4035.0 21 4593.0 22 5385.0 23 6374.0 24 7521.0 25 9243.0 26 11725.0 27 14778.5 28 17978.0 29 22041.0 30 26960.5 31 32120.5 32 38502.5 33 43953.5 34 48665.0 35 53206.0 36 58475.5 37 63935.5 38 66660.5 39 68184.5 40 70442.5 41 74147.5 42 77473.5 43 80828.0 44 84852.0 45 88806.5 46 90600.0 47 99121.0 48 120461.0 49 119523.0 50 99008.0 51 88595.0 52 83650.5 53 81750.5 54 80376.0 55 75847.0 56 69337.5 57 62838.0 58 57820.5 59 51902.0 60 43431.0 61 35694.0 62 29488.5 63 23265.0 64 17170.0 65 12903.0 66 9809.0 67 7754.0 68 5732.0 69 3906.0 70 2937.5 71 2255.5 72 1756.0 73 1454.0 74 1159.5 75 856.0 76 607.5 77 385.0 78 226.0 79 136.0 80 96.5 81 72.0 82 50.5 83 36.5 84 21.5 85 13.0 86 9.0 87 5.0 88 2.5 89 2.5 90 2.0 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.028462138359232 2 0.01210898891947322 3 0.0042902709085550815 4 0.011908508970475318 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004651134816751302 10-11 2.4057593879748117E-4 12-13 2.00479948997901E-5 14-15 6.014398469937029E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0015236476123840475 24-25 0.0 26-27 6.014398469937029E-5 28-29 0.0 30-31 2.00479948997901E-5 32-33 0.0037890710360603285 34-35 8.21967790891394E-4 36-37 0.002505999362473762 38-39 1.2028796939874058E-4 40-41 0.0029871512400687244 42-43 0.011046445189784343 44-45 0.00206494347467838 46-47 0.0020248474848788 48-49 0.010485101332590221 50-51 3.207679183966416E-4 52-53 0.0019246075103798493 54-55 0.010845965240786442 56-57 0.0043704628881542406 58-59 0.0014635036276846771 60-61 0.005753774536239758 62-63 0.0034081591329643162 64-65 0.01856444327720563 66-67 0.002967103245168934 68-69 2.8067192859706136E-4 70-71 0.0011627837041878256 72-73 0.0 74-75 2.00479948997901E-5 76-77 0.0013031196684863563 78-79 0.011708029021477417 80-81 0.016258923863729766 82-83 0.012068892929673638 84-85 0.008420157857911842 86-87 0.011026397194884555 88-89 0.029310168543493123 90-91 0.02497980164513846 92-93 0.0028267672808704038 94-95 0.008760973771208273 96-97 0.011387261103080774 98-99 0.011206829148982664 100-101 0.011467453082679935 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2494015.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.67634884793773 #Duplication Level Percentage of deduplicated Percentage of total 1 78.41254824541257 45.22549486658163 2 12.91244949342942 14.894858829288241 3 3.8376990629371313 6.640334097820971 4 1.616745347629922 3.7299187467273494 5 0.8726873928698851 2.5166711253180387 6 0.4991795286378887 1.7274511578880798 7 0.3300842101633143 1.3326636438202715 8 0.23692879305246656 1.0932150176171926 9 0.17705251396477775 0.9190568303853199 >10 0.9833281743161748 10.47096123549331 >50 0.0803854951526485 3.209333355091178 >100 0.03736292461531346 4.038905438506234 >500 0.0018092012407855693 0.7100087017409753 >1k 0.0014612779252480234 2.0522466205126344 >5k 2.087539893211462E-4 0.8668519557338886 >10k+ 6.958466310704874E-5 0.572028377474657 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14253 0.5714881426134165 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8256 0.3310324917853341 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7327 0.2937833172615241 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6016 0.24121747463427445 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4835 0.19386411068097023 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4339 0.1739764997403785 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4253 0.17052824461761457 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4004 0.1605443431575191 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3681 0.1475933384522547 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3638 0.14586921089087274 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2865 0.11487501077579725 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2794 0.11202819550002706 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2615 0.10485101332590221 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 4.00959897995802E-5 0.0 0.0 0.0 6 0.0 4.00959897995802E-5 0.0 0.0 0.0 7 0.0 4.00959897995802E-5 0.0 0.0 0.0 8 0.0 4.00959897995802E-5 0.0 0.0 0.0 9 0.0 4.00959897995802E-5 0.0 0.0 0.0 10-11 0.0 4.00959897995802E-5 0.0 0.0 0.0 12-13 0.0 4.00959897995802E-5 0.0 0.0 0.0 14-15 0.0 4.00959897995802E-5 0.0 0.0 0.0 16-17 2.00479948997901E-5 8.01919795991604E-5 0.0 0.0 0.0 18-19 4.00959897995802E-5 8.01919795991604E-5 0.0 0.0 0.0 20-21 4.00959897995802E-5 8.01919795991604E-5 0.0 0.0 0.0 22-23 4.00959897995802E-5 8.01919795991604E-5 0.0 2.00479948997901E-5 0.0 24-25 8.01919795991604E-5 8.01919795991604E-5 0.0 1.603839591983208E-4 0.0 26-27 8.01919795991604E-5 8.01919795991604E-5 0.0 3.207679183966415E-4 0.0 28-29 8.01919795991604E-5 8.01919795991604E-5 0.0 5.813918520939129E-4 0.0 30-31 1.2028796939874058E-4 8.01919795991604E-5 0.0 0.0011828316990876158 0.0 32-33 1.2028796939874058E-4 8.01919795991604E-5 0.0 0.001864463525680479 0.0 34-35 1.4033596429853068E-4 8.01919795991604E-5 0.0 0.002646335326772293 0.0 36-37 2.0047994899790098E-4 8.01919795991604E-5 0.0 0.00406974296465739 0.0 38-39 2.0047994899790098E-4 8.01919795991604E-5 0.0 0.005312718648444376 0.0 40-41 2.0047994899790098E-4 8.01919795991604E-5 0.0 0.007036846209826324 0.0 42-43 3.0071992349685144E-4 8.01919795991604E-5 0.0 0.009562893567199877 0.0 44-45 3.207679183966416E-4 8.01919795991604E-5 0.0 0.014153884399251809 0.0 46-47 3.608639081962218E-4 8.01919795991604E-5 0.0 0.025200329589036154 0.0 48-49 4.0095989799580195E-4 8.01919795991604E-5 0.0 0.046050244284817854 0.0 50-51 4.0095989799580195E-4 8.01919795991604E-5 0.0 0.0623492641383472 0.0 52-53 4.0095989799580195E-4 8.01919795991604E-5 0.0 0.07822727609898096 0.0 54-55 4.0095989799580195E-4 8.01919795991604E-5 0.0 0.101623286147036 4.00959897995802E-5 56-57 4.0095989799580195E-4 8.01919795991604E-5 0.0 0.12004739345994311 4.00959897995802E-5 58-59 4.0095989799580195E-4 8.01919795991604E-5 0.0 0.1447665711713843 4.00959897995802E-5 60-61 4.0095989799580195E-4 8.01919795991604E-5 0.0 0.1819355537155951 4.00959897995802E-5 62-63 4.0095989799580195E-4 8.01919795991604E-5 0.0 0.20918077878440988 4.00959897995802E-5 64-65 4.0095989799580195E-4 8.01919795991604E-5 0.0 0.24125757062407405 4.00959897995802E-5 66-67 4.0095989799580195E-4 1.2028796939874058E-4 0.0 0.3031056348899265 4.00959897995802E-5 68-69 4.0095989799580195E-4 1.2028796939874058E-4 0.0 0.3722912652891021 4.00959897995802E-5 70-71 4.0095989799580195E-4 1.2028796939874058E-4 0.0 0.42888675489120953 4.00959897995802E-5 72-73 4.0095989799580195E-4 2.0047994899790098E-4 0.0 0.49412293029512655 4.00959897995802E-5 74-75 4.0095989799580195E-4 2.0047994899790098E-4 0.0 0.5522621155045178 4.00959897995802E-5 76-77 4.0095989799580195E-4 2.4057593879748117E-4 0.0 0.6009586951161079 4.00959897995802E-5 78-79 4.0095989799580195E-4 2.4057593879748117E-4 0.0 0.6698235575968869 4.00959897995802E-5 80-81 5.813918520939128E-4 2.4057593879748117E-4 0.0 0.7774812902087598 4.00959897995802E-5 82-83 6.415358367932831E-4 2.4057593879748117E-4 0.0 0.8828335034071567 4.00959897995802E-5 84-85 6.615838316930732E-4 2.4057593879748117E-4 0.0 1.011942590561805 4.00959897995802E-5 86-87 6.816318265928633E-4 2.4057593879748117E-4 0.0 1.239046276786627 4.00959897995802E-5 88-89 6.816318265928633E-4 2.4057593879748117E-4 0.0 1.52721615547621 4.00959897995802E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCTG 2215 0.0 24.92205 1 TACCTGG 2180 0.0 23.970076 2 GTACATG 10915 0.0 22.51676 1 GAGTACT 9630 0.0 22.141409 12-13 TACATGG 10960 0.0 21.758371 2 ACATGGG 10960 0.0 20.672714 3 GTACTTT 10215 0.0 20.594465 14-15 AGTACTT 9940 0.0 19.038254 12-13 AGAGTAC 16110 0.0 18.629736 10-11 CGTATAC 230 2.0600019E-6 18.586788 3 ACTTTTT 11555 0.0 18.268747 16-17 GTATAGA 840 0.0 17.715038 1 TAAGGTG 1195 0.0 17.485876 5 ATTCGCG 345 0.0 17.209126 80-81 GTATTAG 785 0.0 17.121746 1 CATGGGG 5235 0.0 16.875622 4 TAGCCCT 1405 0.0 16.56469 4 TACTTTT 11195 0.0 16.564644 14-15 GAGTACA 9180 0.0 16.471289 1 GTAAGGT 1105 0.0 16.333712 4 >>END_MODULE