FastQCFastQC Report
Thu 26 May 2016
SRR936585_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936585_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2506174
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120020.4788973151904058No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT79580.31753581355484495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73990.2952308977748552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63160.25201761729233485No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT52400.2090836470253063No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC50120.1999861142921441No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35620.1421289982259811No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33670.1343482136515661No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33070.13195412609020762No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28430.11343984894903547No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC27400.10932999863537007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG109950.027.0157591
TACATGG111000.025.9472122
GTACCTG21100.025.0018921
ACATGGG111550.024.5837023
TACCTGG22100.022.5807342
GTGTAGC13650.021.238781
GAGTACT76950.021.03238712-13
CATGGGG52500.020.7316824
TATCACG1456.1252655E-419.6663742
CATGGGA60950.019.6510244
AGAGTAC137200.019.62318410-11
GTACTTT82700.019.39780814-15
AGTACTT80850.019.01841512-13
GTATAGA8750.018.4672931
GAGTACA87800.017.5381151
GTATAGG7450.017.2242341
ACTTTTT102200.016.64809416-17
ATGGGAG30050.016.6061335
TATACCG1750.002163029716.2943465
GTATCAA240850.016.1807841
Produced by FastQC (version 0.11.5)