##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936585_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2506174 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.26434597118955 31.0 31.0 34.0 28.0 34.0 2 31.29981158530892 31.0 31.0 34.0 28.0 34.0 3 31.350993985254018 31.0 31.0 34.0 28.0 34.0 4 34.97399302682096 37.0 35.0 37.0 32.0 37.0 5 34.94997394434704 37.0 35.0 37.0 32.0 37.0 6 35.07287642438234 37.0 35.0 37.0 32.0 37.0 7 34.93213440088358 37.0 35.0 37.0 32.0 37.0 8 34.88057652820594 37.0 35.0 37.0 32.0 37.0 9 36.339514335397304 39.0 37.0 39.0 32.0 39.0 10-11 36.23917852471536 39.0 35.0 39.0 32.0 39.0 12-13 36.129194740668446 39.0 35.0 39.0 31.0 39.0 14-15 37.15181647403572 39.0 36.0 41.0 31.0 41.0 16-17 37.02748472372629 39.0 36.0 41.0 31.0 41.0 18-19 36.97166338011647 39.0 36.0 41.0 31.0 41.0 20-21 36.883855031613926 39.0 36.0 41.0 30.5 41.0 22-23 36.73988338399488 39.0 36.0 40.5 30.0 41.0 24-25 36.874514299485995 39.0 36.0 41.0 30.0 41.0 26-27 36.85235881467129 39.0 36.0 41.0 30.0 41.0 28-29 36.76059084484956 39.0 36.0 41.0 30.0 41.0 30-31 36.57936320462984 39.0 36.0 41.0 30.0 41.0 32-33 36.403415325512114 39.0 35.0 40.5 30.0 41.0 34-35 36.17386143180801 39.0 35.0 40.0 29.5 41.0 36-37 35.98207347135514 38.0 35.0 40.0 29.0 41.0 38-39 35.78605595621054 38.0 35.0 40.0 27.5 41.0 40-41 35.61032194891496 38.0 35.0 40.0 27.0 41.0 42-43 35.38713054241246 38.0 34.5 40.0 27.0 41.0 44-45 35.11968003817772 38.0 34.0 40.0 26.0 41.0 46-47 34.845439502604364 38.0 34.0 40.0 24.5 41.0 48-49 34.74333685530215 38.0 34.0 40.0 24.0 41.0 50-51 33.82814521258301 37.5 33.0 39.5 23.5 40.5 52-53 33.98916196560973 37.5 33.0 39.5 23.5 40.5 54-55 34.41269301333427 38.0 34.0 40.0 24.0 41.0 56-57 34.30767536491879 38.0 33.5 40.0 23.0 41.0 58-59 34.03920078972968 38.0 33.0 40.0 23.0 41.0 60-61 33.70227426347891 37.0 33.0 40.0 22.0 41.0 62-63 33.37923703621536 37.0 32.5 40.0 21.5 41.0 64-65 33.0399118337354 36.0 32.0 39.0 20.5 41.0 66-67 32.63028085839211 36.0 31.5 39.0 19.5 41.0 68-69 32.20593981104265 35.0 31.0 39.0 18.0 40.0 70-71 31.749080670376436 35.0 31.0 38.0 15.5 40.0 72-73 31.353909185874564 35.0 31.0 37.0 13.0 39.5 74-75 30.919565840201038 35.0 30.0 37.0 9.5 39.0 76-77 30.483199091523574 34.5 30.0 36.0 7.5 39.0 78-79 30.029441491293102 34.0 29.5 36.0 6.5 38.5 80-81 29.525096222369235 34.0 29.0 35.0 2.0 37.0 82-83 29.02770378273815 34.0 29.0 35.0 2.0 37.0 84-85 28.6977027532805 34.0 28.5 35.0 2.0 36.0 86-87 28.391047070155544 34.0 28.0 35.0 2.0 36.0 88-89 27.972166936533537 34.0 27.0 35.0 2.0 35.5 90-91 27.540609111737652 33.0 26.0 35.0 2.0 35.0 92-93 27.14311416525748 33.0 25.5 35.0 2.0 35.0 94-95 26.658459867511194 33.0 25.0 35.0 2.0 35.0 96-97 26.055940648973294 33.0 24.5 35.0 2.0 35.0 98-99 25.330635462661412 32.0 21.0 35.0 2.0 35.0 100-101 22.85918615387439 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.35975375495194584 1101 2 0.3595013696153053 1101 3 0.31620463225443274 1101 4 0.3907308401318872 1101 5 0.3548530432136019 1101 6 0.19365805460243735 1101 7 0.39075946383092486 1101 8 0.4094086166373003 1101 9 0.6064191873698022 1101 10-11 0.5440570870471291 1101 12-13 0.6483237648738864 1101 14-15 0.7562181306443208 1101 16-17 0.7303696201920076 1101 18-19 0.7765455754026078 1101 20-21 0.7786774805332186 1101 22-23 0.7906127019621039 1101 24-25 0.8341152500260591 1101 26-27 0.5968325517849209 1101 28-29 0.692722323321874 1101 30-31 0.7975353752039283 1101 32-33 0.5796577986996923 1101 34-35 0.4859118735826158 1101 36-37 0.3994456187153972 1101 38-39 0.38702210258872327 1101 40-41 0.2912552170490059 1101 42-43 0.44983177744011726 1101 44-45 0.433480022262529 1101 46-47 0.5391147916835166 1101 48-49 0.665502662097964 1101 50-51 0.5271437649529673 1101 52-53 0.40656599023158435 1101 54-55 0.44080887332562924 1101 56-57 0.36467460188004 1101 58-59 0.23940664171061599 1101 60-61 0.10401352360033655 1101 62-63 0.3123819796792162 1101 64-65 0.3163578106442273 1101 66-67 0.34595871564946634 1101 68-69 0.2875735577989573 1101 70-71 0.344500124800156 1101 72-73 0.2920499579368503 1101 74-75 0.16552412718971965 1101 76-77 0.10011178353270367 1101 78-79 0.05230341228099533 1101 80-81 0.30096335010351893 1101 82-83 0.402838618471435 1101 84-85 0.2946547404557407 1101 86-87 0.10999258687991542 1101 88-89 0.22626460259759895 1101 90-91 0.10651879483912907 1101 92-93 0.05459011533693214 1101 94-95 0.1144042036055204 1101 96-97 -0.011900235442844576 1101 98-99 -0.03781044247667964 1101 100-101 0.6042271838321511 1102 1 0.438962718968984 1102 2 0.42925423223955406 1102 3 0.5058270528713713 1102 4 0.4656473783958788 1102 5 0.48629810197670764 1102 6 0.45348841201504797 1102 7 0.5382129367455164 1102 8 0.42765916801347714 1102 9 0.6441348283257184 1102 10-11 0.5662988843823982 1102 12-13 0.4743619382325974 1102 14-15 0.5514464608053586 1102 16-17 0.44213545903910045 1102 18-19 0.6601202302260134 1102 20-21 0.5605530658983398 1102 22-23 0.48785412164245656 1102 24-25 0.3814388096502199 1102 26-27 0.33013246265835505 1102 28-29 0.343036909865269 1102 30-31 0.284516477352021 1102 32-33 0.2817496292685888 1102 34-35 0.10442753870844967 1102 36-37 0.33736179443067016 1102 38-39 0.2955508723400797 1102 40-41 0.3162391667151567 1102 42-43 0.2031868636964944 1102 44-45 0.03272357946969606 1102 46-47 0.20402951720392792 1102 48-49 0.4294436044225094 1102 50-51 0.6247921078032093 1102 52-53 0.4866144811225439 1102 54-55 0.5546355750386738 1102 56-57 0.6082106603712276 1102 58-59 0.7379241573455317 1102 60-61 0.5940254952611141 1102 62-63 0.7093835884891462 1102 64-65 0.6236193593314994 1102 66-67 0.5393471110692687 1102 68-69 0.4637035013017794 1102 70-71 0.4655798779649061 1102 72-73 0.4333106455709199 1102 74-75 0.35098497948132845 1102 76-77 0.5676527213253841 1102 78-79 0.32388846772073876 1102 80-81 0.2788879097578878 1102 82-83 0.6806878359704527 1102 84-85 0.61624791547613 1102 86-87 0.4417697931399225 1102 88-89 0.5298387287041244 1102 90-91 0.6135988803402022 1102 92-93 0.4176868101011273 1102 94-95 0.5598997246182442 1102 96-97 0.30080898537953615 1102 98-99 0.4237529733364873 1102 100-101 0.5290723453173918 1103 1 0.37858726455229075 1103 2 0.3807949212218169 1103 3 0.3760379730356682 1103 4 0.42244139976723716 1103 5 0.4761120862078556 1103 6 0.31960269683006004 1103 7 0.30914659584250614 1103 8 0.3402341172604437 1103 9 0.5524410146877088 1103 10-11 0.5830832862834754 1103 12-13 0.5299237231656377 1103 14-15 0.45499307490918994 1103 16-17 0.5566720720413159 1103 18-19 0.45352882755381074 1103 20-21 0.6536671776022231 1103 22-23 0.6470538958111334 1103 24-25 0.7088942335364052 1103 26-27 0.49566751372613993 1103 28-29 0.24962280445634377 1103 30-31 0.2948303592902377 1103 32-33 0.5523341153646157 1103 34-35 0.2832287048479145 1103 36-37 0.3146094849709371 1103 38-39 0.35427144344940587 1103 40-41 0.17550228381696087 1103 42-43 0.2708251165331603 1103 44-45 0.31444914326114315 1103 46-47 0.24846792898807735 1103 48-49 0.2802724722757688 1103 50-51 0.34068429891788554 1103 52-53 0.48304799858316017 1103 54-55 0.41105071911433555 1103 56-57 0.5299034510798819 1103 58-59 0.38681595109149924 1103 60-61 0.26441060849152365 1103 62-63 0.4422008342911994 1103 64-65 0.3882787238563097 1103 66-67 0.3014393163475759 1103 68-69 0.4508627871397124 1103 70-71 0.4845645615649872 1103 72-73 0.4171965574730976 1103 74-75 0.42373345295598597 1103 76-77 0.44854735173314 1103 78-79 0.6037791519817866 1103 80-81 0.445756211537784 1103 82-83 0.3559584204565489 1103 84-85 0.44362661966069794 1103 86-87 0.5197952050657051 1103 88-89 0.6611966280793524 1103 90-91 0.7073764207459128 1103 92-93 0.7267816356523262 1103 94-95 0.6714054176135384 1103 96-97 0.7702066767066711 1103 98-99 0.7689365498122562 1103 100-101 1.0842426630549866 1104 1 0.205615440319189 1104 2 0.38169548293308964 1104 3 0.3990443343251435 1104 4 0.37426416706542653 1104 5 0.5167689913150255 1104 6 0.3899924777832524 1104 7 0.489357720451423 1104 8 0.4507957157795417 1104 9 0.6531448450899262 1104 10-11 0.6760475927627141 1104 12-13 0.5085678605922368 1104 14-15 0.5853682566638199 1104 16-17 0.5208053144850808 1104 18-19 0.7132619534196891 1104 20-21 0.8341258708906167 1104 22-23 0.5920735493326319 1104 24-25 0.7677162453824451 1104 26-27 0.3403291363648009 1104 28-29 0.6829562358366701 1104 30-31 0.6594996982279397 1104 32-33 0.4150791593337928 1104 34-35 0.4057224396444994 1104 36-37 0.3666771614236737 1104 38-39 0.6323364172365373 1104 40-41 0.6253533259095221 1104 42-43 0.6068195067739524 1104 44-45 0.6383645718392899 1104 46-47 0.534557008120828 1104 48-49 0.5155464254034214 1104 50-51 0.39604678358578127 1104 52-53 0.5342545159634682 1104 54-55 0.6257119446626405 1104 56-57 0.5034492669731065 1104 58-59 0.675838228841009 1104 60-61 0.7159290011285862 1104 62-63 0.8287769373935063 1104 64-65 0.6363342066936752 1104 66-67 0.8165915612807524 1104 68-69 0.8060177675842439 1104 70-71 0.8314872706232563 1104 72-73 0.5985384091299437 1104 74-75 0.45731111941773506 1104 76-77 0.8416703595406254 1104 78-79 0.6926900728490359 1104 80-81 0.6495999113806157 1104 82-83 0.7544725426564511 1104 84-85 0.9005455637141004 1104 86-87 1.004615629530786 1104 88-89 0.9701796208464266 1104 90-91 0.9512569396890846 1104 92-93 0.8549138696826795 1104 94-95 0.901400949121431 1104 96-97 1.0088518534314872 1104 98-99 1.1664122929840204 1104 100-101 1.6482429047408438 1105 1 0.18130394242505332 1105 2 0.23227512645346238 1105 3 0.28179870687487707 1105 4 0.38206443469823625 1105 5 0.3843961927618196 1105 6 0.32720383184750546 1105 7 0.2505887403691247 1105 8 0.327120983715794 1105 9 0.45908222750748706 1105 10-11 0.3880496880060136 1105 12-13 0.2696966596430741 1105 14-15 0.1245488132308381 1105 16-17 0.29169042202371287 1105 18-19 0.22218797813172841 1105 20-21 0.24912760807640666 1105 22-23 0.1453734816658354 1105 24-25 0.34101410674743704 1105 26-27 0.2805634971411166 1105 28-29 0.055875405871510964 1105 30-31 0.04825722350437189 1105 32-33 0.15332205280258648 1105 34-35 -0.03376223822512259 1105 36-37 0.06340833154347081 1105 38-39 0.14200396994382203 1105 40-41 -0.004294025375237709 1105 42-43 0.20587778518490296 1105 44-45 -0.20608352651121464 1105 46-47 -0.07386291899322828 1105 48-49 0.16909999444782642 1105 50-51 0.21942183213595712 1105 52-53 0.08826373594428816 1105 54-55 0.10583918403926162 1105 56-57 0.29792975739051997 1105 58-59 0.24153741957034924 1105 60-61 0.16643380911266092 1105 62-63 0.21696930834108485 1105 64-65 0.3018442034772235 1105 66-67 0.3735875649948426 1105 68-69 0.2728824658449298 1105 70-71 0.3548655052783687 1105 72-73 0.4426984304920154 1105 74-75 0.48614290369718915 1105 76-77 0.3537822995447506 1105 78-79 0.3287680459401052 1105 80-81 0.16656933387633543 1105 82-83 0.2255324687388125 1105 84-85 0.41090381845684476 1105 86-87 0.36170270274444505 1105 88-89 0.056522685454563515 1105 90-91 0.06485789066555014 1105 92-93 0.14277143410268422 1105 94-95 0.08788348168038951 1105 96-97 0.33621408473663905 1105 98-99 0.3648662355613048 1105 100-101 0.749316963474417 1106 1 0.035574434916032516 1106 2 0.1520654821880072 1106 3 0.16543827395265254 1106 4 0.11090336833360936 1106 5 0.15092757123316858 1106 6 0.08374223691504312 1106 7 0.18559272560278828 1106 8 0.19342373562451343 1106 9 0.3557744835692347 1106 10-11 0.2807255102226449 1106 12-13 0.39636233000133103 1106 14-15 0.5432466381322527 1106 16-17 0.5690577032553605 1106 18-19 0.34711299281416785 1106 20-21 0.20417548331761282 1106 22-23 0.15297819892399644 1106 24-25 -5.3726124448871815E-5 1106 26-27 0.023943289908970655 1106 28-29 0.3156486670511356 1106 30-31 0.2304364121946989 1106 32-33 0.126960256510948 1106 34-35 0.18258849379937914 1106 36-37 0.08504159565958247 1106 38-39 0.21652364441315086 1106 40-41 0.09974338338388833 1106 42-43 -0.11693767752169748 1106 44-45 0.2086117604256117 1106 46-47 0.253557848269125 1106 48-49 0.16120084183435068 1106 50-51 -0.12433105191471583 1106 52-53 0.10627096180216711 1106 54-55 0.27161153281012673 1106 56-57 0.20126398215769825 1106 58-59 0.17616516728408982 1106 60-61 0.379318960971041 1106 62-63 0.3117932239380323 1106 64-65 0.0916726811179629 1106 66-67 0.1593010266946493 1106 68-69 0.07942270621744996 1106 70-71 0.13561446702012603 1106 72-73 0.2418300690522983 1106 74-75 0.08810274722432965 1106 76-77 0.18669010431025157 1106 78-79 0.3094556560584003 1106 80-81 0.36359037171508035 1106 82-83 0.5031632473276204 1106 84-85 0.3690279270229979 1106 86-87 0.3416221066373879 1106 88-89 0.49819721771398306 1106 90-91 0.5892532887694166 1106 92-93 0.7608777664292212 1106 94-95 0.608709211607394 1106 96-97 0.800825996390298 1106 98-99 0.9538279248808799 1106 100-101 1.4830931296796344 1107 1 0.11091087343492134 1107 2 0.2124024677613967 1107 3 0.10417311924042849 1107 4 0.17766225275923375 1107 5 0.2773190434349857 1107 6 0.15464466844206015 1107 7 0.27185280052320593 1107 8 0.295811758777937 1107 9 0.21230344880262209 1107 10-11 0.3071434233920627 1107 12-13 0.30468667601069654 1107 14-15 0.23979785024872768 1107 16-17 0.25000952598885817 1107 18-19 0.4094949202174263 1107 20-21 0.511278334774083 1107 22-23 0.6119471574885225 1107 24-25 0.48860726680551636 1107 26-27 0.23071686665550573 1107 28-29 0.3205401158077592 1107 30-31 0.335697383242497 1107 32-33 0.24250481019262082 1107 34-35 0.46264926894955494 1107 36-37 0.3694401222594621 1107 38-39 0.11769251753297993 1107 40-41 0.23969464343275604 1107 42-43 0.5143689491318426 1107 44-45 0.43847416319318455 1107 46-47 0.4577428152114038 1107 48-49 0.4544042106480788 1107 50-51 0.3764759837271825 1107 52-53 0.502382576550751 1107 54-55 0.5629891723481038 1107 56-57 0.6115738755081139 1107 58-59 0.7690758619391005 1107 60-61 0.6990363682452951 1107 62-63 0.5618145851138365 1107 64-65 0.6715935666168491 1107 66-67 0.6914985706804373 1107 68-69 0.8324700602971689 1107 70-71 0.8424025016356467 1107 72-73 0.6247666576204232 1107 74-75 0.7424642043691243 1107 76-77 0.5812333659012943 1107 78-79 0.5575128310689514 1107 80-81 0.8148231981112453 1107 82-83 1.0133402845846398 1107 84-85 0.7970834869202577 1107 86-87 0.8606431916809143 1107 88-89 0.6816249830744923 1107 90-91 0.8319708210847061 1107 92-93 0.7595710876752584 1107 94-95 0.6849235423513385 1107 96-97 0.9159650784849447 1107 98-99 1.0295165622983653 1107 100-101 1.4435220725098326 1108 1 0.11197728397746332 1108 2 0.17493890087719421 1108 3 0.1239519801840494 1108 4 0.266419470750094 1108 5 0.28190136081701667 1108 6 0.11356195627933374 1108 7 -0.06540436681509476 1108 8 0.04155713644116332 1108 9 0.22333952193719142 1108 10-11 0.1495157267228393 1108 12-13 0.2877410722282292 1108 14-15 0.2092280591306377 1108 16-17 0.2431439764626333 1108 18-19 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76-77 -2.5466690935368455 2316 78-79 -3.443192018143666 2316 80-81 -2.6325455612148865 2316 82-83 -2.445120566111946 2316 84-85 -2.466874330675193 2316 86-87 -3.0453628091781866 2316 88-89 -3.2618381831981544 2316 90-91 -3.1615216424918344 2316 92-93 -3.55379946269629 2316 94-95 -4.167533289205053 2316 96-97 -5.309605861874601 2316 98-99 -6.481929917543983 2316 100-101 -6.525563023214332 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6067.0 3 2567.0 4 2537.0 5 2871.0 6 3113.0 7 3650.0 8 4543.0 9 5755.0 10 7212.0 11 8986.0 12 10957.0 13 13005.0 14 14589.0 15 17062.0 16 19961.0 17 24422.0 18 21570.0 19 16669.0 20 17596.0 21 19274.0 22 21164.0 23 24163.0 24 27708.0 25 31875.0 26 36631.0 27 43349.0 28 51324.0 29 61680.0 30 74250.0 31 90357.0 32 112474.0 33 142465.0 34 185422.0 35 247612.0 36 337388.0 37 428028.0 38 322094.0 39 45764.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.80282661027496 18.285553257790482 14.113413266819222 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22.557941321879742 26.24827771134023 28.25712567629632 22.93665529048371 46-47 23.005054137507734 26.692142514986543 27.91107686666218 22.391726480843534 48-49 22.803518073981383 26.6596013636886 27.82102704393682 22.715853518393196 50-51 22.173016905707147 27.010446836013024 28.1749549261611 22.64158133211873 52-53 22.838920930885852 27.25632719707145 27.304051018201292 22.600700853841406 54-55 22.35575066954323 26.690029267763222 27.714490765062006 23.239729297631545 56-57 22.828811103972033 26.80936398791589 27.416402942009842 22.945421966102238 58-59 23.335455655016244 26.648592014816213 27.3110277390969 22.704924591070647 60-61 22.782009135262705 26.79470879454598 28.16978691038009 22.25349515981123 62-63 22.431096195975307 26.969170361089052 28.01428953364485 22.585443909290788 64-65 22.225864340942213 27.330860106397303 27.631318275178586 22.8119572774819 66-67 22.504088697756 27.06142294463254 27.158370522509333 23.276117835102124 68-69 22.33101226621714 26.88187620124003 27.69438937098123 23.092722161561596 70-71 22.805953091858004 26.794759271204544 27.413408203547807 22.985879433389645 72-73 22.67005810589622 26.584524885938947 27.938533522417575 22.806883485747257 74-75 22.26820163095775 27.583728090975786 27.311409661368668 22.83666061669779 76-77 22.48961047848762 26.933566319853806 27.323508820094727 23.253314381563843 78-79 22.621327590929976 27.066595824360828 27.3331323518761 22.978944232833097 80-81 22.896435916145354 27.090844262901836 27.135618032655724 22.877101788297086 82-83 22.80366525991853 26.91455282009373 27.5489262638387 22.73285565614904 84-85 22.50387658308359 27.199834939671767 27.324488212214266 22.971800265030375 86-87 22.494682378216435 27.296034463735726 27.24160627791614 22.967676880131695 88-89 22.394114126097367 27.230327214684756 26.85534716679968 23.5202114924182 90-91 22.549296190538513 26.83166831333128 27.205759123027395 23.41327637310281 92-93 22.90396635754586 26.95429036071854 27.364425000374137 22.77731828136146 94-95 22.491241155108025 26.758059144924438 27.51794717696728 23.23275252300026 96-97 22.036524288570092 26.840006759988295 27.963326065880356 23.16014288556126 98-99 22.173742645083866 27.13014694466916 27.11645781092792 23.579652599319058 100-101 21.98928407344118 26.75532872459877 27.362794746856896 23.892592455103156 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 172.0 1 139.0 2 175.5 3 252.5 4 241.5 5 257.0 6 416.0 7 832.5 8 1388.0 9 1760.0 10 1748.0 11 1613.0 12 1698.0 13 1873.5 14 2067.5 15 2301.5 16 2529.0 17 2713.5 18 2924.0 19 3209.5 20 3560.0 21 4111.5 22 4862.0 23 5766.0 24 7042.0 25 8833.5 26 11366.5 27 14528.0 28 17559.0 29 21443.0 30 26666.5 31 32224.0 32 38300.5 33 43330.5 34 47860.0 35 52651.0 36 58540.5 37 63990.0 38 66707.0 39 68675.0 40 71284.5 41 75064.0 42 78040.0 43 81494.5 44 85573.5 45 89210.5 46 91461.5 47 100086.5 48 119758.0 49 119376.5 50 100237.5 51 90134.0 52 85909.0 53 83407.0 54 80855.0 55 76476.5 56 70684.0 57 63771.0 58 58227.0 59 52063.0 60 43910.5 61 36681.0 62 30390.0 63 24008.0 64 17837.0 65 13390.5 66 10172.0 67 7936.0 68 5828.0 69 4065.5 70 3097.5 71 2332.5 72 1830.5 73 1516.0 74 1167.5 75 867.0 76 635.5 77 394.5 78 225.0 79 144.5 80 108.5 81 76.0 82 49.0 83 32.5 84 25.0 85 21.5 86 16.0 87 13.0 88 10.5 89 8.0 90 6.5 91 4.5 92 5.0 93 3.5 94 3.0 95 3.5 96 1.5 97 0.0 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03355712731837454 2 0.02366156539809287 3 0.02162659097093817 4 0.05147288256920708 5 0.0550640139112448 6 0.04899899208913667 7 0.05039554316659578 8 0.04293397026702855 9 0.04293397026702855 10-11 0.051812044973732874 12-13 0.0464452986903543 14-15 0.040440129057280144 16-17 0.0497371690872222 18-19 0.05263002489053034 20-21 0.011631275402266564 22-23 0.021247527107056415 24-25 0.00600516963307416 26-27 0.010593837459011225 28-29 0.006344332037599943 30-31 0.009097532733162182 32-33 0.003770687909139589 34-35 0.008439158653788604 36-37 0.009157384922196143 38-39 0.010952950593214996 40-41 0.0016958120226289156 42-43 0.002134728075544635 44-45 0.0024339890207144433 46-47 3.790638638817576E-4 48-49 0.0011970437806792346 50-51 3.3916240452578315E-4 52-53 0.003511328423325755 54-55 0.006962804657617547 56-57 0.016120189579813694 58-59 0.010793344755791098 60-61 0.011172408619672856 62-63 0.005745810147260326 64-65 5.985218903396173E-5 66-67 0.007202213413753395 68-69 0.017157627523069027 70-71 0.0059253667143622105 72-73 0.0023940875613584693 74-75 0.004010096665275436 76-77 0.006523888604701828 78-79 0.005526352120802466 80-81 0.009676103893823812 82-83 0.006304430578243968 84-85 0.016898268037255193 86-87 0.004768224393038951 88-89 0.006942853927939561 90-91 0.003910343016885499 92-93 0.016419450524983503 94-95 0.011112556430638895 96-97 0.010075118487383556 98-99 0.021427083674158298 100-101 0.03714825866041224 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2506174.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.63301338747431 #Duplication Level Percentage of deduplicated Percentage of total 1 79.91161155840406 47.653702018769565 2 12.042076307710538 14.362105953413796 3 3.54704746104345 6.345633861912326 4 1.5117674357290785 3.6060499093431937 5 0.8081649489277606 2.4096655609348314 6 0.45787498208606964 1.6382678961916892 7 0.299061577482769 1.2483760137596405 8 0.2245447478300605 1.0712223962749634 9 0.17257410248622326 0.9262002387503083 >10 0.9138605826249442 10.041437577521355 >50 0.0727564956870337 2.994319007603083 >100 0.035376995501172154 3.828260151265417 >500 0.001674900248367966 0.6572460677223433 >1k 0.0012059281788213145 1.4625532823002605 >5k 3.3498004967258735E-4 1.2754592858783822 >10k+ 6.699600993451748E-5 0.4795007783590397 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12002 0.4788973151904058 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7958 0.31753581355484495 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7399 0.2952308977748552 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6316 0.25201761729233485 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5240 0.2090836470253063 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5012 0.1999861142921441 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3562 0.1421289982259811 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3367 0.1343482136515661 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3307 0.13195412609020762 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2843 0.11343984894903547 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2740 0.10932999863537007 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.9901459355974485E-5 0.0 0.0 0.0 0.0 6 3.9901459355974485E-5 0.0 0.0 0.0 3.9901459355974485E-5 7 3.9901459355974485E-5 0.0 0.0 0.0 3.9901459355974485E-5 8 7.980291871194897E-5 0.0 0.0 0.0 3.9901459355974485E-5 9 7.980291871194897E-5 0.0 0.0 0.0 3.9901459355974485E-5 10-11 7.980291871194897E-5 0.0 0.0 0.0 3.9901459355974485E-5 12-13 7.980291871194897E-5 0.0 0.0 1.9950729677987243E-5 3.9901459355974485E-5 14-15 1.1970437806792346E-4 0.0 0.0 3.9901459355974485E-5 3.9901459355974485E-5 16-17 1.1970437806792346E-4 0.0 0.0 3.9901459355974485E-5 3.9901459355974485E-5 18-19 1.1970437806792346E-4 0.0 0.0 3.9901459355974485E-5 3.9901459355974485E-5 20-21 1.1970437806792346E-4 0.0 0.0 3.9901459355974485E-5 3.9901459355974485E-5 22-23 1.1970437806792346E-4 0.0 0.0 5.9852189033961724E-5 3.9901459355974485E-5 24-25 1.1970437806792346E-4 0.0 0.0 1.1970437806792346E-4 3.9901459355974485E-5 26-27 1.1970437806792346E-4 0.0 0.0 1.396551077459107E-4 3.9901459355974485E-5 28-29 1.396551077459107E-4 0.0 0.0 5.7857116066163E-4 3.9901459355974485E-5 30-31 1.5960583742389794E-4 0.0 0.0 0.0012768466993911835 3.9901459355974485E-5 32-33 1.7955656710188519E-4 0.0 0.0 0.0023541861020024944 3.9901459355974485E-5 34-35 2.1945802645785968E-4 0.0 0.0 0.0035512298826817293 3.9901459355974485E-5 36-37 2.3940875613584692E-4 0.0 0.0 0.0049477809601408355 3.9901459355974485E-5 38-39 2.3940875613584692E-4 0.0 0.0 0.006563790064057803 3.9901459355974485E-5 40-41 2.3940875613584692E-4 0.0 0.0 0.008778321058314387 3.9901459355974485E-5 42-43 2.5935948581383417E-4 0.0 0.0 0.012030289995826306 3.9901459355974485E-5 44-45 2.793102154918214E-4 0.0 0.0 0.016738662199831295 3.9901459355974485E-5 46-47 2.793102154918214E-4 0.0 0.0 0.028310085413063898 3.9901459355974485E-5 48-49 2.793102154918214E-4 0.0 0.0 0.049896774924646094 5.9852189033961724E-5 50-51 2.793102154918214E-4 0.0 0.0 0.06675514150254531 7.980291871194897E-5 52-53 2.793102154918214E-4 0.0 0.0 0.08377311391786843 7.980291871194897E-5 54-55 2.793102154918214E-4 0.0 0.0 0.10801325047662294 7.980291871194897E-5 56-57 2.793102154918214E-4 0.0 0.0 0.12750511337201648 7.980291871194897E-5 58-59 2.793102154918214E-4 0.0 0.0 0.1521642152540087 7.980291871194897E-5 60-61 2.992609451698086E-4 0.0 0.0 0.19156690636803353 7.980291871194897E-5 62-63 3.192116748477959E-4 0.0 0.0 0.22089447899467474 7.980291871194897E-5 64-65 3.192116748477959E-4 1.9950729677987243E-5 0.0 0.2554890442563046 7.980291871194897E-5 66-67 3.9901459355974486E-4 7.980291871194897E-5 0.0 0.3186730051464902 7.980291871194897E-5 68-69 4.3891605291571936E-4 7.980291871194897E-5 0.0 0.39099440022919396 7.980291871194897E-5 70-71 4.3891605291571936E-4 1.1970437806792346E-4 0.0 0.44875176264696703 7.980291871194897E-5 72-73 4.3891605291571936E-4 1.1970437806792346E-4 0.0 0.515965770932106 7.980291871194897E-5 74-75 4.3891605291571936E-4 1.1970437806792346E-4 0.0 0.5764164818564075 7.980291871194897E-5 76-77 4.3891605291571936E-4 1.1970437806792346E-4 0.0 0.6286275414237 7.980291871194897E-5 78-79 4.588667825937066E-4 1.9950729677987243E-4 0.0 0.6992531244837749 7.980291871194897E-5 80-81 4.7881751227169385E-4 2.793102154918214E-4 0.0 0.8105981468166217 7.980291871194897E-5 82-83 4.7881751227169385E-4 2.793102154918214E-4 0.0 0.9215641052855867 7.980291871194897E-5 84-85 4.987682419496811E-4 2.793102154918214E-4 0.0 1.05098448870669 7.980291871194897E-5 86-87 5.187189716276683E-4 2.793102154918214E-4 0.0 1.2787220679809144 7.980291871194897E-5 88-89 5.187189716276683E-4 2.793102154918214E-4 0.0 1.5697433617937142 7.980291871194897E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 10995 0.0 27.015759 1 TACATGG 11100 0.0 25.947212 2 GTACCTG 2110 0.0 25.001892 1 ACATGGG 11155 0.0 24.583702 3 TACCTGG 2210 0.0 22.580734 2 GTGTAGC 1365 0.0 21.23878 1 GAGTACT 7695 0.0 21.032387 12-13 CATGGGG 5250 0.0 20.731682 4 TATCACG 145 6.1252655E-4 19.666374 2 CATGGGA 6095 0.0 19.651024 4 AGAGTAC 13720 0.0 19.623184 10-11 GTACTTT 8270 0.0 19.397808 14-15 AGTACTT 8085 0.0 19.018415 12-13 GTATAGA 875 0.0 18.467293 1 GAGTACA 8780 0.0 17.538115 1 GTATAGG 745 0.0 17.224234 1 ACTTTTT 10220 0.0 16.648094 16-17 ATGGGAG 3005 0.0 16.606133 5 TATACCG 175 0.0021630297 16.294346 5 GTATCAA 24085 0.0 16.180784 1 >>END_MODULE