FastQCFastQC Report
Thu 26 May 2016
SRR936585_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936585_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2506174
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT145980.5824815036785156No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82050.3273914740157707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75840.3026126677557105No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT63440.25313485815430214No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48400.1931230632829165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43360.17301272776750537No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC42960.17141666939326639No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT41310.1648329285995306No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC35960.14348564784408424No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35530.14176988509177735No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29720.11858713720595618No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28400.11332014457096755No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27870.1112053672251009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG23050.024.326061
GTACATG109450.023.4877911
TACCTGG23200.023.3392492
TACATGG109950.023.111522
ACATGGG111300.022.1048323
GAGTACT96450.021.79249812-13
GTACTTT99950.020.86262914-15
GCACCGT6650.019.9985146
AGTACTT99950.019.50861212-13
CATGGGG52150.019.4892354
CGTACAC2752.8443537E-818.9982093
AGAGTAC158300.018.9202210-11
TACATCG2552.6811722E-718.6253225
TAACGCG1051.958514E-518.09461476-77
TAAGGTG11650.017.9378645
ACTTTTT118200.017.8610516-17
GAGTACA90650.017.5081141
TACTTTT110500.017.17282914-15
GTGTAGC13800.016.872431
AGTACAT87000.016.4876082
Produced by FastQC (version 0.11.5)