FastQCFastQC Report
Thu 26 May 2016
SRR936584_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936584_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537862
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT118730.46783473648291357No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT78910.3109310120093212No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74060.2918204378331052No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61050.2405568151459772No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT52200.20568494268009846No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC48440.1908693222878155No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT36770.14488573452772452No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT33960.13381342247923644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33800.13318297054764996No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29210.11509688076026198No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC27360.10780728030129298No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC25630.10099051879101385No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACG300.003960118631.65374464-65
TACCTGG22000.025.496012
GTACCTG24900.024.4365921
GTACATG108850.023.8448311
TACATGG110200.023.3792512
GAGTACT83150.022.0673812-13
ACATGGG111850.022.0169963
GTACTTT87300.020.63333114-15
GTACACG4500.020.0710981
GTGTAGC15250.019.6381571
AGTACTT86250.019.53812412-13
AGAGTAC149250.019.0645710-11
CATGGGA60300.019.0012054
ACTTTTT105300.017.6011916-17
TAGCCCT17500.017.3869484
CATGGGG51000.017.1525654
CGCGGAA3050.017.12533882-83
GTATCAA250950.016.8969251
GTACCGT2555.463802E-616.7800146
TACTTTT100800.016.45543114-15
Produced by FastQC (version 0.11.5)