##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936584_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2537862 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.220005264273627 31.0 31.0 34.0 28.0 34.0 2 31.276151737170895 31.0 31.0 34.0 28.0 34.0 3 31.22367922290495 31.0 31.0 34.0 27.0 34.0 4 34.88737567290893 37.0 35.0 37.0 32.0 37.0 5 34.923697978849916 37.0 35.0 37.0 32.0 37.0 6 34.98209713530523 37.0 35.0 37.0 32.0 37.0 7 34.84829632186462 37.0 35.0 37.0 32.0 37.0 8 34.83259058215143 37.0 35.0 37.0 32.0 37.0 9 36.29247531977704 39.0 37.0 39.0 32.0 39.0 10-11 36.16217587875148 39.0 35.0 39.0 31.5 39.0 12-13 36.05843718058744 39.0 35.0 39.0 30.5 39.0 14-15 37.01295184686953 39.0 36.0 41.0 31.0 41.0 16-17 36.92673202877067 39.0 36.0 41.0 31.0 41.0 18-19 36.801977609499644 39.0 36.0 41.0 30.0 41.0 20-21 36.777940447510545 39.0 36.0 41.0 30.0 41.0 22-23 36.76946402129036 39.0 36.0 41.0 30.0 41.0 24-25 36.8834916949779 39.0 36.0 41.0 30.0 41.0 26-27 36.80424861556696 39.0 36.0 41.0 30.0 41.0 28-29 36.69179037315662 39.0 36.0 41.0 30.0 41.0 30-31 36.50897842357071 39.0 36.0 41.0 30.0 41.0 32-33 36.3462434127624 39.0 35.0 40.5 30.0 41.0 34-35 36.143409688942896 39.0 35.0 40.0 29.5 41.0 36-37 35.933553518670436 38.0 35.0 40.0 28.5 41.0 38-39 35.73965014646187 38.0 35.0 40.0 27.5 41.0 40-41 35.52493496494293 38.0 35.0 40.0 27.0 41.0 42-43 35.253225943727436 38.0 34.0 40.0 26.0 41.0 44-45 35.09952806732596 38.0 34.0 40.0 26.0 41.0 46-47 34.96176249930059 38.0 34.0 40.0 25.0 41.0 48-49 34.71864151793912 38.0 34.0 40.0 24.0 41.0 50-51 33.738238525183796 37.5 32.5 39.5 23.5 40.5 52-53 33.86003848909043 37.5 33.0 39.5 23.0 40.5 54-55 34.30915688087059 38.0 33.5 40.0 23.0 41.0 56-57 34.145019705563186 38.0 33.0 40.0 23.0 41.0 58-59 33.90956442864112 38.0 33.0 40.0 22.5 41.0 60-61 33.60332338795411 37.0 33.0 40.0 22.0 41.0 62-63 33.276427362874735 37.0 32.0 40.0 20.5 41.0 64-65 32.9162710580796 36.0 32.0 39.0 20.0 41.0 66-67 32.52777258968376 36.0 31.5 39.0 18.5 41.0 68-69 32.07569619624708 35.0 31.0 39.0 17.5 40.0 70-71 31.663136923914696 35.0 31.0 38.0 14.0 40.0 72-73 31.24165380150694 35.0 30.5 37.0 12.0 39.5 74-75 30.822324657526693 35.0 30.0 37.0 8.0 39.0 76-77 30.35674910613737 34.0 30.0 36.0 7.0 39.0 78-79 29.882418153548144 34.0 29.0 36.0 4.5 38.5 80-81 29.41127255934326 34.0 29.0 35.0 2.0 37.0 82-83 28.995413659213938 34.0 29.0 35.0 2.0 37.0 84-85 28.61193437625844 34.0 28.5 35.0 2.0 36.0 86-87 28.278057081117886 34.0 27.0 35.0 2.0 36.0 88-89 27.914757579411333 34.0 27.0 35.0 2.0 36.0 90-91 27.531430984033015 33.0 26.0 35.0 2.0 35.0 92-93 27.079825853415198 33.0 25.5 35.0 2.0 35.0 94-95 26.574884883417617 33.0 25.0 35.0 2.0 35.0 96-97 25.961400383472387 33.0 24.0 35.0 2.0 35.0 98-99 25.204764679876213 32.0 19.0 35.0 2.0 35.0 100-101 22.65026014022827 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5541519752074038 1101 2 0.5298529310063209 1101 3 0.6057169512756921 1101 4 0.5776919335479676 1101 5 0.5719725397349578 1101 6 0.5810750818820054 1101 7 0.5870408332111055 1101 8 0.5881745839755155 1101 9 0.7363719151621737 1101 10-11 0.733548464471852 1101 12-13 0.7284607821701314 1101 14-15 0.8926257621331075 1101 16-17 0.9734541582027347 1101 18-19 1.0109951764992857 1101 20-21 0.9559895705780121 1101 22-23 0.8430710091084279 1101 24-25 0.7704980335724017 1101 26-27 0.7861428767675704 1101 28-29 0.7566621550761496 1101 30-31 0.6134912844399523 1101 32-33 0.5209315942316337 1101 34-35 0.7233704858946837 1101 36-37 0.6413101923000042 1101 38-39 0.6707353031228038 1101 40-41 0.5719905220949144 1101 42-43 0.6703417179422502 1101 44-45 0.5508409716845719 1101 46-47 0.7243781946163281 1101 48-49 0.7717808941764517 1101 50-51 0.71857043866666 1101 52-53 0.6856374874419302 1101 54-55 0.620705060412206 1101 56-57 0.7053249241811699 1101 58-59 0.6656550959109637 1101 60-61 0.538136571112922 1101 62-63 0.6999407126339392 1101 64-65 0.5295337204564987 1101 66-67 0.626665279628611 1101 68-69 0.7055953525527627 1101 70-71 0.5251767025633889 1101 72-73 0.6334995350499284 1101 74-75 0.3885754778407886 1101 76-77 0.4919667839034041 1101 78-79 0.5862893593181617 1101 80-81 0.7098664625479465 1101 82-83 0.5280980703836455 1101 84-85 0.5144547333446283 1101 86-87 0.4914501626718142 1101 88-89 0.6545217814006286 1101 90-91 0.555427298973175 1101 92-93 0.5722571852123721 1101 94-95 0.6617056720621584 1101 96-97 0.652418772655377 1101 98-99 0.455058324792585 1101 100-101 0.7397661913454137 1102 1 0.2861217755002663 1102 2 0.3532321861459309 1102 3 0.522412702515556 1102 4 0.34789028056258786 1102 5 0.44699813561515356 1102 6 0.33998665958024077 1102 7 0.5014937551968615 1102 8 0.3711024282377622 1102 9 0.5411591714397943 1102 10-11 0.5195271005686237 1102 12-13 0.5520229675248558 1102 14-15 0.6797495838023551 1102 16-17 0.45015395237504663 1102 18-19 0.6978786059042577 1102 20-21 0.781143286238823 1102 22-23 0.41091091647037103 1102 24-25 0.5357032966219393 1102 26-27 0.3680966737861695 1102 28-29 0.24090395238423667 1102 30-31 0.3907851650748668 1102 32-33 0.532208443389699 1102 34-35 0.1992049178283608 1102 36-37 0.3589308891193781 1102 38-39 0.2859455262514885 1102 40-41 0.48045335300862035 1102 42-43 0.3024049174615868 1102 44-45 0.5644482469664709 1102 46-47 0.6193404387433148 1102 48-49 0.730775139860711 1102 50-51 0.7390284879392794 1102 52-53 0.5681348966584423 1102 54-55 0.8474919211313079 1102 56-57 0.7070054863484287 1102 58-59 0.5294551279799009 1102 60-61 0.5874811855588007 1102 62-63 0.60967525793194 1102 64-65 0.7191013325745672 1102 66-67 0.6165759170954885 1102 68-69 0.6900641904654847 1102 70-71 0.3607410316705639 1102 72-73 0.7363640899498911 1102 74-75 0.6204125726194789 1102 76-77 0.7733188203746515 1102 78-79 0.6220271976096008 1102 80-81 0.8543270523793467 1102 82-83 0.6758462880306695 1102 84-85 0.6820460752300939 1102 86-87 0.49801562292660506 1102 88-89 0.6729299765744408 1102 90-91 0.8007562458079924 1102 92-93 0.884650520113798 1102 94-95 0.9402652151608777 1102 96-97 0.9416667560244498 1102 98-99 0.911817040961882 1102 100-101 1.5100520104478967 1103 1 0.4135987701075372 1103 2 0.49235976784193625 1103 3 0.5369940765022534 1103 4 0.4321130583515256 1103 5 0.3665926977167828 1103 6 0.35514876108337745 1103 7 0.574480125144639 1103 8 0.34784995238452154 1103 9 0.5639319687993734 1103 10-11 0.4966975087884151 1103 12-13 0.42478002923841274 1103 14-15 0.7717366397877754 1103 16-17 0.8857068944538682 1103 18-19 0.9674504928486627 1103 20-21 0.8457182381340118 1103 22-23 0.7357095032450047 1103 24-25 0.616831539397694 1103 26-27 0.6070606899856585 1103 28-29 0.6820381485301183 1103 30-31 0.6757608798293049 1103 32-33 0.7377132410877252 1103 34-35 0.5540644322938917 1103 36-37 0.5796248158904902 1103 38-39 0.4942114580604482 1103 40-41 0.558907570800919 1103 42-43 0.632135183530977 1103 44-45 0.48470157564910465 1103 46-47 0.7580808687616951 1103 48-49 0.8585031743293854 1103 50-51 0.7141064509127233 1103 52-53 0.9007132842204015 1103 54-55 0.7851874068575242 1103 56-57 0.8031109596139672 1103 58-59 0.7304899673697776 1103 60-61 0.786482078564319 1103 62-63 0.7705904698883543 1103 64-65 0.813199339169536 1103 66-67 0.8091444310722125 1103 68-69 0.8768464913030343 1103 70-71 0.9200580254986654 1103 72-73 0.7213187426790988 1103 74-75 0.8180639741876874 1103 76-77 0.7217069971520012 1103 78-79 0.7277938705461757 1103 80-81 0.8192718680470605 1103 82-83 0.5676030618153902 1103 84-85 0.5692291632760522 1103 86-87 0.47364448569289763 1103 88-89 0.450264437273983 1103 90-91 0.7224125123387886 1103 92-93 0.6479495150412582 1103 94-95 0.852805520633293 1103 96-97 1.0781241477300298 1103 98-99 1.229796401394612 1103 100-101 1.700428491105228 1104 1 0.37156772524755155 1104 2 0.31484000686466374 1104 3 0.3903358383043596 1104 4 0.6284736887577722 1104 5 0.44924247863062305 1104 6 0.47028740759375864 1104 7 0.4773340983212151 1104 8 0.5509753294594475 1104 9 0.5777849136307225 1104 10-11 0.7005409265685216 1104 12-13 0.6791211408958162 1104 14-15 0.7354077782253796 1104 16-17 0.6644015983858864 1104 18-19 0.5982763567216907 1104 20-21 0.7717899413510523 1104 22-23 0.5925535819755439 1104 24-25 0.72575256932776 1104 26-27 0.395196359517648 1104 28-29 0.25309932516597655 1104 30-31 0.3074126616039976 1104 32-33 0.3065763523092997 1104 34-35 0.3824712535905448 1104 36-37 0.49446954656669817 1104 38-39 0.24426305541737037 1104 40-41 0.44860394782001123 1104 42-43 0.7139194255360835 1104 44-45 0.6446139411853196 1104 46-47 0.720364911761294 1104 48-49 0.667216374067884 1104 50-51 0.4249599054142976 1104 52-53 0.3626571268457681 1104 54-55 0.4688766989201696 1104 56-57 0.5996702353419536 1104 58-59 0.5547823271789554 1104 60-61 0.4814680401211362 1104 62-63 0.4109489679640319 1104 64-65 0.4378452264189576 1104 66-67 0.4825169531834348 1104 68-69 0.42705441154235046 1104 70-71 0.3692698376014363 1104 72-73 0.621378714476144 1104 74-75 0.4719861837431978 1104 76-77 0.48188392295806537 1104 78-79 0.33544576758198374 1104 80-81 0.5068604074906737 1104 82-83 0.48331008968156297 1104 84-85 0.452952493385677 1104 86-87 0.3816452276863025 1104 88-89 0.4632212269638778 1104 90-91 0.5466222083196008 1104 92-93 0.5460172705652404 1104 94-95 0.5042020710843396 1104 96-97 0.4870666090230067 1104 98-99 0.5279430387417356 1104 100-101 1.207343977696219 1105 1 0.24869417616948297 1105 2 0.234444579379403 1105 3 0.4298301021574673 1105 4 0.40532394589538967 1105 5 0.3738179362198011 1105 6 0.1880312300192415 1105 7 0.24784082345895087 1105 8 0.26279750890580544 1105 9 0.1603131842958021 1105 10-11 0.34009549474134815 1105 12-13 0.26021555575093913 1105 14-15 0.5747362655035744 1105 16-17 0.45926029330392026 1105 18-19 0.2565540894722389 1105 20-21 0.37867120696987655 1105 22-23 0.1612443631335836 1105 24-25 0.08367583328701045 1105 26-27 0.18460402636763718 1105 28-29 0.24215439265856986 1105 30-31 0.17845301966692517 1105 32-33 0.23298668943456136 1105 34-35 0.15095513062762933 1105 36-37 0.12367390343318618 1105 38-39 -0.023036987519311936 1105 40-41 0.06173533404747644 1105 42-43 -0.0922004377419654 1105 44-45 -0.10454427081572959 1105 46-47 0.23025343319586256 1105 48-49 0.30427790135921384 1105 50-51 0.3234335427778916 1105 52-53 0.4995793511585376 1105 54-55 0.3941328113660205 1105 56-57 0.22538345453127562 1105 58-59 0.3052835163801433 1105 60-61 0.13576442137951972 1105 62-63 0.26648990977277265 1105 64-65 0.3916822696740354 1105 66-67 0.23308773356957602 1105 68-69 0.3304646811267773 1105 70-71 0.573125092761078 1105 72-73 0.3876215623838277 1105 74-75 0.419672789430134 1105 76-77 0.32303655560344424 1105 78-79 0.32263055690281206 1105 80-81 0.6897736006285271 1105 82-83 0.49166742413414255 1105 84-85 0.5289120557210474 1105 86-87 0.7024889936669503 1105 88-89 0.6995539162470727 1105 90-91 0.6216670144291498 1105 92-93 0.6530942237015473 1105 94-95 0.6643781235153305 1105 96-97 0.7730816754542467 1105 98-99 0.9879095939660196 1105 100-101 1.4573193132294513 1106 1 0.16806829265991752 1106 2 -0.0029095991239493912 1106 3 0.05528821822905172 1106 4 0.1762963299799054 1106 5 0.3274296845458622 1106 6 0.12570114502780427 1106 7 0.2678552844138835 1106 8 0.4021943518316178 1106 9 0.43096149367782743 1106 10-11 0.4401875542566813 1106 12-13 0.4867043239083344 1106 14-15 0.2263687463693529 1106 16-17 0.16605485757494165 1106 18-19 0.35061149989964235 1106 20-21 0.0513356997263017 1106 22-23 -0.0516086649894163 1106 24-25 -0.32267045592433163 1106 26-27 -0.14004465435503732 1106 28-29 0.03424780616141021 1106 30-31 -0.07086398744380773 1106 32-33 0.09535207845024729 1106 34-35 0.012893368824713036 1106 36-37 -0.14161584165066898 1106 38-39 0.03332977148158278 1106 40-41 0.031075491775133912 1106 42-43 0.21339673473656262 1106 44-45 -0.06983610044890298 1106 46-47 0.0018474856373060788 1106 48-49 0.09642015633740897 1106 50-51 -0.002683957449960417 1106 52-53 0.04256815131133607 1106 54-55 0.14212600846354917 1106 56-57 0.4325250599057213 1106 58-59 0.3478548006952167 1106 60-61 0.35769457104046154 1106 62-63 0.22983207981770448 1106 64-65 0.08612539444255418 1106 66-67 0.07011966741082887 1106 68-69 0.1824388341695382 1106 70-71 0.025709473378451975 1106 72-73 0.1482093762054575 1106 74-75 0.2757835003293181 1106 76-77 0.11061225383051365 1106 78-79 0.1192591983196074 1106 80-81 0.3004758343927918 1106 82-83 0.25555646114992214 1106 84-85 0.4916618865332083 1106 86-87 0.47859355029785533 1106 88-89 0.5319305181989868 1106 90-91 0.7098271499927229 1106 92-93 0.7447258004546491 1106 94-95 0.9911288661966218 1106 96-97 0.9534096138446628 1106 98-99 1.1663450160824063 1106 100-101 1.5699198200173257 1107 1 0.15487578298717608 1107 2 0.2851628105950432 1107 3 0.34337216995125885 1107 4 0.30758240850856566 1107 5 0.3797303484394803 1107 6 0.3726075575363552 1107 7 0.3945475254344757 1107 8 0.32357407327739196 1107 9 0.3970736913269377 1107 10-11 0.40706711477567836 1107 12-13 0.37213359084565667 1107 14-15 0.20496758805719395 1107 16-17 0.2913030857872769 1107 18-19 0.5543805771176906 1107 20-21 0.24239637014596838 1107 22-23 0.27003263912710196 1107 24-25 0.2629128967558856 1107 26-27 0.21927882555412737 1107 28-29 0.2909020415295416 1107 30-31 0.27523590354437744 1107 32-33 0.32401172605606376 1107 34-35 0.3204189232406307 1107 36-37 0.17982023122121404 1107 38-39 0.2668126539093194 1107 40-41 0.24042452640077983 1107 42-43 0.20835143658196387 1107 44-45 0.18393690225253323 1107 46-47 0.12497697477021319 1107 48-49 0.28692266269542444 1107 50-51 0.2506728117605945 1107 52-53 0.17884271969752774 1107 54-55 0.25595300369418084 1107 56-57 0.321938482077222 1107 58-59 0.3773795454876989 1107 60-61 0.5310813085814416 1107 62-63 0.5403125674000151 1107 64-65 0.42326165752905354 1107 66-67 0.40203467690832895 1107 68-69 0.3756441530612662 1107 70-71 0.4298275278728134 1107 72-73 0.434664703182591 1107 74-75 0.334768126260812 1107 76-77 0.49148188846719876 1107 78-79 0.5234395315396476 1107 80-81 0.480121042832355 1107 82-83 0.5097521296044363 1107 84-85 0.40913968951770485 1107 86-87 0.5542283574688511 1107 88-89 0.6724500790690477 1107 90-91 0.5880529445183775 1107 92-93 0.7218259250481687 1107 94-95 0.6984147677479022 1107 96-97 0.8470862432238455 1107 98-99 1.0340019425407299 1107 100-101 1.3518006062730095 1108 1 0.21663602482362165 1108 2 0.17459821066431758 1108 3 0.3638042006846405 1108 4 0.3961144486886852 1108 5 0.2710688249958295 1108 6 0.3249578425128945 1108 7 0.5268476797311692 1108 8 0.4543127450662894 1108 9 0.01703817159900467 1108 10-11 0.1716754845839077 1108 12-13 0.20229871979360325 1108 14-15 0.6706023142592841 1108 16-17 0.710781877864612 1108 18-19 0.779256730406729 1108 20-21 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96-97 -5.794284179092635 2316 98-99 -7.529113017163457 2316 100-101 -7.934600335381184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6722.0 3 2703.0 4 2721.0 5 2909.0 6 3309.0 7 3848.0 8 4862.0 9 6041.0 10 7691.0 11 9502.0 12 11565.0 13 13621.0 14 15099.0 15 17773.0 16 21006.0 17 25327.0 18 21219.0 19 17824.0 20 18312.0 21 19518.0 22 21955.0 23 24685.0 24 28262.0 25 32764.0 26 37573.0 27 44597.0 28 52681.0 29 63565.0 30 76319.0 31 92514.0 32 114502.0 33 143882.0 34 186211.0 35 247798.0 36 338944.0 37 430106.0 38 324434.0 39 45481.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.75567769337754 18.320357832797633 14.140278942700773 22.78368553112405 2 15.551775200987903 20.068164627784636 39.92857179456961 24.451488376657853 3 20.192585831691932 24.174156422194425 29.362166823121967 26.27109092299168 4 12.689827171159354 16.19216114086268 37.52410034572074 33.59391134225723 5 13.729917680324078 37.39518883548756 33.94104167537055 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22.621799779575724 52-53 22.864251216676116 27.231577665723485 27.25695340596417 22.647217711636237 54-55 22.38336011449043 26.69355683975516 27.65921522672305 23.263867819031354 56-57 22.856228664739245 26.839230990807245 27.410069277657968 22.894471066795546 58-59 23.3629591392426 26.574229686357874 27.31813229357328 22.74467888082624 60-61 22.745582802854607 26.80924458476268 28.17416869883579 22.271003913546924 62-63 22.453127096432393 26.991321397899398 28.01847352828353 22.537077977384683 64-65 22.265741209569537 27.345168834188062 27.58820968183636 22.80088027440604 66-67 22.44687673313704 27.077946960958055 27.156458376636106 23.3187179292688 68-69 22.337971331900448 26.887105738762223 27.670471124533226 23.104451804804103 70-71 22.82411931594294 26.77774056269209 27.423378516825597 22.974761604539363 72-73 22.661177968222564 26.619267853870298 27.910721288280815 22.80883288962632 74-75 22.21110646951258 27.656437196544548 27.308015164890598 22.824441169052275 76-77 22.562167264600944 26.92263049041121 27.301078055302924 23.21412418968492 78-79 22.61006561964254 27.0104242615327 27.373775789701853 23.005734329122905 80-81 22.88312696548626 27.072735799238636 27.13261662870339 22.91152060657172 82-83 22.754413977193828 26.939210334889314 27.600233121359448 22.706142566557407 84-85 22.526518371057414 27.198870346355402 27.284378523025538 22.990232759561646 86-87 22.493136834207903 27.299835331305612 27.261495384035843 22.945532450450642 88-89 22.46104967047447 27.21135559302873 26.86830465020014 23.45929008629666 90-91 22.603047415938345 26.86796777070179 27.175668747692296 23.35331606566757 92-93 22.918812326766787 26.952610623453378 27.35730666596784 22.771270383812002 94-95 22.478335969128683 26.778866294042842 27.50889556299682 23.233902173831655 96-97 22.07786833966982 26.808824645007345 27.95741171857528 23.155895296747556 98-99 22.205714848753573 27.157178047098235 27.06475514829047 23.57235195585772 100-101 22.040215105887825 26.753390834156036 27.323965310333005 23.88242874962313 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 152.0 1 133.0 2 181.0 3 267.5 4 252.0 5 244.0 6 412.0 7 801.5 8 1331.0 9 1700.5 10 1691.0 11 1598.0 12 1720.0 13 1917.5 14 2090.0 15 2263.5 16 2416.0 17 2627.0 18 2880.5 19 3179.0 20 3551.0 21 4109.5 22 4921.5 23 5924.5 24 7193.0 25 8940.0 26 11406.0 27 14655.5 28 17617.5 29 21515.5 30 27051.5 31 32694.0 32 39019.0 33 44382.5 34 49116.0 35 53781.5 36 59396.5 37 64748.0 38 67105.5 39 69162.0 40 71793.0 41 75453.5 42 79078.0 43 82500.5 44 86321.5 45 90233.0 46 92770.5 47 101421.5 48 120818.5 49 120298.5 50 101946.0 51 91699.5 52 86765.0 53 84958.0 54 82768.5 55 77658.5 56 71560.0 57 65068.0 58 59149.5 59 52901.5 60 44835.5 61 37013.5 62 30549.0 63 24025.0 64 17838.0 65 13525.0 66 10244.0 67 7997.0 68 5887.5 69 4085.0 70 3053.0 71 2340.0 72 1826.5 73 1523.5 74 1208.0 75 881.5 76 631.0 77 388.5 78 247.0 79 163.0 80 97.5 81 64.5 82 49.5 83 38.0 84 28.5 85 23.5 86 17.5 87 9.0 88 5.5 89 4.0 90 6.5 91 7.5 92 4.0 93 2.0 94 3.5 95 2.0 96 0.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07600886100189845 2 0.03030109596187657 3 0.015603685306766089 4 0.02182939813118286 5 0.026794207092426615 6 0.031916629036567 7 0.07853066872824449 8 0.05496752778519872 9 0.0725019721324485 10-11 0.06178428929547784 12-13 0.04072325445591605 14-15 0.04525462771419408 16-17 0.056996794939992795 18-19 0.05329288984192206 20-21 0.019701622862078397 22-23 0.013692627889144484 24-25 0.023523737697321603 26-27 0.02551360160639152 28-29 0.006915269624589517 30-31 0.0011623957488626252 32-33 0.01006752928252206 34-35 0.01191948183155743 36-37 0.004826897601209207 38-39 0.009673496825280492 40-41 0.004551074881140109 42-43 0.002403597989173564 44-45 5.122421944140382E-4 46-47 6.698551773106654E-4 48-49 0.0010047827659659982 50-51 0.0011426941260005469 52-53 1.9701622862078395E-4 54-55 3.152259657932543E-4 56-57 0.004058534309588149 58-59 0.0038221148352432085 60-61 0.005457349532795715 62-63 0.00269912233210474 64-65 6.698551773106654E-4 66-67 5.713470630002734E-4 68-69 0.0036842034752086602 70-71 0.002443001234897721 72-73 0.02076551049663063 74-75 0.016628169695594167 76-77 0.02578942432646062 78-79 0.020174461810768277 80-81 0.012687845123178488 82-83 0.005043615452692069 84-85 0.0036645018523465815 86-87 0.001536726583242115 88-89 0.0018322509261732907 90-91 0.006737955018830811 92-93 0.010973803934177667 94-95 0.014224571706420601 96-97 0.012195304551626526 98-99 0.024114786383183956 100-101 0.01997744558214749 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2537862.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.745829189475444 #Duplication Level Percentage of deduplicated Percentage of total 1 79.82524728136411 47.6922558908002 2 12.082420503640385 14.437484632118286 3 3.625165480045092 6.4976555266307034 4 1.4945324720794728 3.5716832717993863 5 0.7904905079742792 2.361425543266648 6 0.48108828776042095 1.7245811199354792 7 0.3058002862262521 1.2789204166876451 8 0.21733245830343717 1.038776634490076 9 0.16124655528366555 0.8670428234432271 >10 0.9055413972781877 9.949126819852596 >50 0.07282282889884951 2.985022387842843 >100 0.03506518861319415 3.759854238411664 >500 0.001656506394188562 0.6557313126279216 >1k 0.001258944859576586 1.6565093603892505 >5k 2.6504102306875495E-4 1.0539047938123178 >10k+ 6.626025576718874E-5 0.4700252278917305 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11873 0.46783473648291357 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7891 0.3109310120093212 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7406 0.2918204378331052 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6105 0.2405568151459772 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5220 0.20568494268009846 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4844 0.1908693222878155 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3677 0.14488573452772452 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3396 0.13381342247923644 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3380 0.13318297054764996 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2921 0.11509688076026198 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2736 0.10780728030129298 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2563 0.10099051879101385 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.940324572415679E-5 0.0 5 0.0 3.940324572415679E-5 0.0 3.940324572415679E-5 0.0 6 3.940324572415679E-5 3.940324572415679E-5 0.0 3.940324572415679E-5 0.0 7 3.940324572415679E-5 3.940324572415679E-5 0.0 3.940324572415679E-5 0.0 8 3.940324572415679E-5 3.940324572415679E-5 0.0 3.940324572415679E-5 0.0 9 3.940324572415679E-5 3.940324572415679E-5 0.0 3.940324572415679E-5 0.0 10-11 1.1820973717247037E-4 3.940324572415679E-5 0.0 3.940324572415679E-5 0.0 12-13 1.5761298289662716E-4 3.940324572415679E-5 0.0 3.940324572415679E-5 0.0 14-15 1.9701622862078395E-4 3.940324572415679E-5 0.0 3.940324572415679E-5 0.0 16-17 1.9701622862078395E-4 3.940324572415679E-5 0.0 3.940324572415679E-5 0.0 18-19 2.1671785148286236E-4 3.940324572415679E-5 0.0 3.940324572415679E-5 0.0 20-21 2.3641947434494074E-4 3.940324572415679E-5 0.0 7.880649144831358E-5 0.0 22-23 2.3641947434494074E-4 3.940324572415679E-5 0.0 7.880649144831358E-5 0.0 24-25 2.3641947434494074E-4 3.940324572415679E-5 0.0 7.880649144831358E-5 1.9701622862078395E-5 26-27 2.3641947434494074E-4 3.940324572415679E-5 0.0 1.3791136003454878E-4 3.940324572415679E-5 28-29 2.3641947434494074E-4 3.940324572415679E-5 0.0 5.122421944140382E-4 3.940324572415679E-5 30-31 2.3641947434494074E-4 3.940324572415679E-5 0.0 0.001339710354621331 3.940324572415679E-5 32-33 2.3641947434494074E-4 3.940324572415679E-5 0.0 0.0026203158406564266 3.940324572415679E-5 34-35 2.3641947434494074E-4 3.940324572415679E-5 0.0 0.0034280823780016407 3.940324572415679E-5 36-37 2.3641947434494074E-4 3.940324572415679E-5 0.0 0.004964808961243756 3.940324572415679E-5 38-39 2.3641947434494074E-4 3.940324572415679E-5 0.0 0.005989293350071832 3.940324572415679E-5 40-41 2.3641947434494074E-4 3.940324572415679E-5 0.0 0.007585124801900182 3.940324572415679E-5 42-43 2.561210972070191E-4 3.940324572415679E-5 0.0 0.010993505557039745 3.940324572415679E-5 44-45 2.7582272006909756E-4 3.940324572415679E-5 0.0 0.01574159666680064 3.940324572415679E-5 46-47 2.7582272006909756E-4 3.940324572415679E-5 0.0 0.02718823954966819 3.940324572415679E-5 48-49 2.7582272006909756E-4 7.880649144831358E-5 0.0 0.04838718574926454 3.940324572415679E-5 50-51 2.7582272006909756E-4 7.880649144831358E-5 0.0 0.06420758890751349 5.9104868586235185E-5 52-53 2.7582272006909756E-4 7.880649144831358E-5 0.0 0.07992948395145205 7.880649144831358E-5 54-55 2.7582272006909756E-4 7.880649144831358E-5 0.0 0.10327590704301495 7.880649144831358E-5 56-57 2.7582272006909756E-4 7.880649144831358E-5 0.0 0.12278051367647255 7.880649144831358E-5 58-59 2.7582272006909756E-4 7.880649144831358E-5 0.0 0.1469938081739669 7.880649144831358E-5 60-61 2.7582272006909756E-4 7.880649144831358E-5 0.0 0.1850376419206403 7.880649144831358E-5 62-63 2.7582272006909756E-4 7.880649144831358E-5 0.0 0.21522052814534437 7.880649144831358E-5 64-65 2.7582272006909756E-4 7.880649144831358E-5 0.0 0.2490088113538088 7.880649144831358E-5 66-67 3.152259657932543E-4 7.880649144831358E-5 0.0 0.3104778746834934 7.880649144831358E-5 68-69 3.5462921151741114E-4 7.880649144831358E-5 0.0 0.3834920890103559 7.880649144831358E-5 70-71 3.5462921151741114E-4 9.850811431039198E-5 0.0 0.44245904623655663 7.880649144831358E-5 72-73 3.743308343794895E-4 1.1820973717247037E-4 0.0 0.5090111282646574 7.880649144831358E-5 74-75 3.940324572415679E-4 1.1820973717247037E-4 0.0 0.5676037546564785 7.880649144831358E-5 76-77 3.940324572415679E-4 1.1820973717247037E-4 0.0 0.6180990140519855 7.880649144831358E-5 78-79 3.940324572415679E-4 1.1820973717247037E-4 0.0 0.6889854531097436 7.880649144831358E-5 80-81 4.728389486898815E-4 1.3791136003454878E-4 0.0 0.8047915922930404 7.880649144831358E-5 82-83 4.728389486898815E-4 1.7731460575870554E-4 0.0 0.9190216016473709 7.880649144831358E-5 84-85 4.728389486898815E-4 1.9701622862078395E-4 0.0 1.053761000401125 7.880649144831358E-5 86-87 4.728389486898815E-4 1.9701622862078395E-4 0.0 1.2869888118424089 7.880649144831358E-5 88-89 5.122421944140382E-4 1.9701622862078395E-4 0.0 1.5732927952741322 7.880649144831358E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGACG 30 0.0039601186 31.653744 64-65 TACCTGG 2200 0.0 25.49601 2 GTACCTG 2490 0.0 24.436592 1 GTACATG 10885 0.0 23.844831 1 TACATGG 11020 0.0 23.379251 2 GAGTACT 8315 0.0 22.06738 12-13 ACATGGG 11185 0.0 22.016996 3 GTACTTT 8730 0.0 20.633331 14-15 GTACACG 450 0.0 20.071098 1 GTGTAGC 1525 0.0 19.638157 1 AGTACTT 8625 0.0 19.538124 12-13 AGAGTAC 14925 0.0 19.06457 10-11 CATGGGA 6030 0.0 19.001205 4 ACTTTTT 10530 0.0 17.60119 16-17 TAGCCCT 1750 0.0 17.386948 4 CATGGGG 5100 0.0 17.152565 4 CGCGGAA 305 0.0 17.125338 82-83 GTATCAA 25095 0.0 16.896925 1 GTACCGT 255 5.463802E-6 16.780014 6 TACTTTT 10080 0.0 16.455431 14-15 >>END_MODULE