FastQCFastQC Report
Thu 26 May 2016
SRR936584_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936584_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537862
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT145880.5748145486239993No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83340.32838664986512267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78540.3094730919175275No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT65910.2597067925679174No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT50510.19902579415271596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43430.17112829618001296No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43190.17018261828263317No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT40630.16009538737724904No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC37820.14902307532876097No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC37020.14587081567082844No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30570.12045572217874731No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28130.11084133022205304No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA28020.11040789451908734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG112850.023.2402211
TACATGG114900.022.5725782
GAGTACT101100.021.9375812-13
ACATGGG113750.021.83733
CATGGGG56900.020.6153434
GTACTTT106950.020.51438514-15
TACCTGG20650.020.4728582
AGTACTT104000.020.25271612-13
GTACCTG22750.019.4224621
AGAGTAC166400.019.25054710-11
GTGTAGC13150.019.1492981
ACTTTTT122550.018.21301716-17
ATTCGCG3300.017.9965980-81
TACTTTT116400.017.76775214-15
TAAGGTG11350.017.1551255
GAGTACA92600.016.880591
GTATAGG7450.016.5813471
CATGGGA59500.016.4420574
AGTACAT88100.016.0675222
TCGCGCT4000.016.03338260-61
Produced by FastQC (version 0.11.5)