##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936584_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2537862 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.847150081446507 31.0 31.0 34.0 30.0 34.0 2 32.00556255619888 31.0 31.0 34.0 30.0 34.0 3 32.091290621791096 33.0 31.0 34.0 30.0 34.0 4 35.79417990418707 37.0 35.0 37.0 35.0 37.0 5 35.626218446865906 37.0 35.0 37.0 33.0 37.0 6 35.71668908711349 37.0 35.0 37.0 33.0 37.0 7 35.6572142220499 37.0 35.0 37.0 33.0 37.0 8 35.62290660406279 37.0 35.0 37.0 33.0 37.0 9 37.241329118762174 39.0 37.0 39.0 33.0 39.0 10-11 37.13137278543908 39.0 37.0 39.0 33.0 39.0 12-13 37.02828759010537 39.0 37.0 39.0 33.0 39.0 14-15 38.13521026754016 40.0 37.0 41.0 33.0 41.0 16-17 38.03693482939577 40.0 37.0 41.0 33.0 41.0 18-19 38.18361616983114 40.0 37.5 41.0 33.0 41.0 20-21 38.2024000911003 40.0 37.5 41.0 33.0 41.0 22-23 38.11609555602314 40.0 37.0 41.0 33.0 41.0 24-25 37.98004619636529 40.0 37.0 41.0 33.0 41.0 26-27 37.78017283839704 40.0 37.0 41.0 32.5 41.0 28-29 37.710699990779645 39.5 37.0 41.0 32.5 41.0 30-31 37.70552141921034 40.0 37.0 41.0 33.0 41.0 32-33 37.559692173963754 40.0 36.5 41.0 32.0 41.0 34-35 37.38874631481144 39.0 36.0 41.0 32.0 41.0 36-37 37.19400798782597 39.0 36.0 41.0 31.0 41.0 38-39 37.033483499102786 39.0 36.0 41.0 31.0 41.0 40-41 36.869640666040944 39.0 35.5 41.0 31.0 41.0 42-43 36.599003413109145 39.0 35.0 40.0 30.0 41.0 44-45 36.33673678868276 39.0 35.0 40.0 30.0 41.0 46-47 35.987591326872774 39.0 35.0 40.0 29.5 41.0 48-49 35.61524937132121 38.0 35.0 40.0 28.0 41.0 50-51 35.32664522342034 38.0 34.5 40.0 27.0 41.0 52-53 34.97578118904811 38.0 34.0 40.0 26.0 41.0 54-55 34.79012728036434 38.0 34.0 40.0 26.0 41.0 56-57 34.4724074831492 38.0 33.5 40.0 26.0 41.0 58-59 34.098625141950194 37.0 33.0 40.0 25.0 41.0 60-61 33.661392739242714 37.0 32.5 39.5 23.0 41.0 62-63 33.340869795126764 36.0 32.0 39.0 23.0 40.0 64-65 32.92292685733109 36.0 31.5 39.0 22.0 40.0 66-67 32.489293153055606 35.0 31.0 38.5 21.5 40.0 68-69 32.11467487199856 35.0 31.0 38.0 21.0 40.0 70-71 31.727241276318413 35.0 31.0 37.5 19.5 39.5 72-73 31.257281522793598 34.5 30.0 37.0 18.0 39.0 74-75 30.765815083720078 34.0 30.0 36.0 17.5 39.0 76-77 29.267764165269824 32.5 28.0 35.0 13.5 37.0 78-79 30.049946963231257 34.0 29.0 35.0 13.5 37.0 80-81 29.815347918838768 34.0 29.0 35.0 9.0 37.0 82-83 29.50715976676431 34.0 29.0 35.0 7.0 36.5 84-85 29.125004038832685 34.0 29.0 35.0 6.0 36.0 86-87 28.758370431489183 33.5 29.0 35.0 2.0 36.0 88-89 28.283438382386436 33.0 27.5 35.0 2.0 35.0 90-91 27.7951066685265 33.0 27.0 35.0 2.0 35.0 92-93 27.16372501735713 32.5 25.5 34.0 2.0 35.0 94-95 26.600213683801563 32.0 25.0 34.0 2.0 35.0 96-97 25.857802157879348 32.0 24.5 34.0 2.0 35.0 98-99 24.952413882236307 32.0 19.5 34.0 2.0 35.0 100-101 21.730478252954654 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2617617870573774 1101 2 0.29175231047186045 1101 3 0.28961005348421764 1101 4 0.20782823441668796 1101 5 0.14184772980468807 1101 6 0.18210715767375518 1101 7 0.19543424462524683 1101 8 0.1410391363799235 1101 9 0.22494367404922855 1101 10-11 0.3446483206200526 1101 12-13 0.30762912772544837 1101 14-15 0.4395628444912276 1101 16-17 0.4051136228401049 1101 18-19 0.30386602421636155 1101 20-21 0.2638615437091616 1101 22-23 0.23367554572981675 1101 24-25 0.1723489292844178 1101 26-27 0.26578419983459867 1101 28-29 0.2872706018073714 1101 30-31 0.3488016080031926 1101 32-33 0.2696168644702155 1101 34-35 0.21859019261970047 1101 36-37 0.3736725001596355 1101 38-39 0.39445417914220826 1101 40-41 0.3736826160633413 1101 42-43 0.42484943749481374 1101 44-45 0.39346131619360847 1101 46-47 0.3919342625311444 1101 48-49 0.3732815530977618 1101 50-51 0.3953342653309804 1101 52-53 0.4328356139937455 1101 54-55 0.4401455920354138 1101 56-57 0.4566633872448804 1101 58-59 0.30688721569369903 1101 60-61 0.49572557428594877 1101 62-63 0.4215055405751613 1101 64-65 0.43129077587999376 1101 66-67 0.16269136394424777 1101 68-69 0.2668171964833377 1101 70-71 0.1492569266960757 1101 72-73 0.33584259446638853 1101 74-75 0.18912723079796834 1101 76-77 -0.02962581442092116 1101 78-79 0.10638446637783616 1101 80-81 0.09462382002443093 1101 82-83 0.011155887444868284 1101 84-85 0.18081958399962517 1101 86-87 0.20282621151806168 1101 88-89 0.18754405663462137 1101 90-91 0.1446306992515538 1101 92-93 0.12495989332208168 1101 94-95 0.08997580708895114 1101 96-97 0.19212505546124703 1101 98-99 0.02380248858899492 1101 100-101 0.5303326502422969 1102 1 0.20797943033971578 1102 2 0.21946180830596163 1102 3 0.22922199436007418 1102 4 0.17194883109861792 1102 5 0.18328803696773122 1102 6 0.07442752014011944 1102 7 0.18120312240577618 1102 8 0.20118399564781697 1102 9 0.2536145537676191 1102 10-11 0.24839664308384357 1102 12-13 0.2794924391741205 1102 14-15 0.3412734166971845 1102 16-17 0.37714245743362795 1102 18-19 0.2997543993505758 1102 20-21 0.18837575230825365 1102 22-23 0.18847169271536046 1102 24-25 0.3132764453703629 1102 26-27 0.2687732870365309 1102 28-29 0.21336230275264967 1102 30-31 0.23855545705582415 1102 32-33 0.22758203410804612 1102 34-35 0.18694781994244636 1102 36-37 0.1723835658778725 1102 38-39 0.2756065022373946 1102 40-41 0.2675218233990222 1102 42-43 0.4472903330043536 1102 44-45 0.39615903773913175 1102 46-47 0.45247836137834696 1102 48-49 0.5674771998328154 1102 50-51 0.4488875225432096 1102 52-53 0.5169488859801561 1102 54-55 0.5380518771108171 1102 56-57 0.24521015891964737 1102 58-59 0.4761322916453068 1102 60-61 0.6178034793481615 1102 62-63 0.5740810694786944 1102 64-65 0.4982369955197896 1102 66-67 0.29902066827910545 1102 68-69 0.453011206080312 1102 70-71 0.36155904605367084 1102 72-73 0.4078018007788522 1102 74-75 0.31704848345816217 1102 76-77 0.31749836911573226 1102 78-79 0.5605981316142952 1102 80-81 0.592671170999008 1102 82-83 0.6209843310968104 1102 84-85 0.4955440937160773 1102 86-87 0.5387557147552151 1102 88-89 0.6106199670587102 1102 90-91 0.4446328829342008 1102 92-93 0.5370200606420816 1102 94-95 0.2694186810149688 1102 96-97 0.46393633287192415 1102 98-99 0.36939091931353474 1102 100-101 1.016515429471358 1103 1 0.14718639030421343 1103 2 0.21579604194956303 1103 3 0.2008417067112518 1103 4 0.20637635490448503 1103 5 0.251668762901474 1103 6 0.21586790699960545 1103 7 0.12652500919799792 1103 8 0.10422380591914049 1103 9 0.24569644973947646 1103 10-11 0.20456535112338514 1103 12-13 0.2718777865788269 1103 14-15 0.341086841242479 1103 16-17 0.3146762647234027 1103 18-19 0.2958954223928032 1103 20-21 0.2397490754879925 1103 22-23 0.16453530977121744 1103 24-25 0.30383980584692694 1103 26-27 0.2152423237288943 1103 28-29 0.301676713743376 1103 30-31 0.3340097728534914 1103 32-33 0.2330650393059841 1103 34-35 0.3077113244442984 1103 36-37 0.15253218098884247 1103 38-39 0.2691738797169805 1103 40-41 0.2672477127065065 1103 42-43 0.2967849965811098 1103 44-45 0.32591511392035244 1103 46-47 0.3502874237712561 1103 48-49 0.3141922239354358 1103 50-51 0.3484727954394913 1103 52-53 0.45584162000756123 1103 54-55 0.2939543270626359 1103 56-57 0.303632686277453 1103 58-59 0.3985671226347236 1103 60-61 0.37120545296949814 1103 62-63 0.3800866685685236 1103 64-65 0.3589295343886505 1103 66-67 0.12569834076705177 1103 68-69 0.2673593759668478 1103 70-71 0.1648444784978338 1103 72-73 0.346538395804199 1103 74-75 0.31691559790592194 1103 76-77 0.3577334724505299 1103 78-79 0.12607802011948266 1103 80-81 0.44104053804862886 1103 82-83 0.46077220119646256 1103 84-85 0.7946729415685105 1103 86-87 0.6115528308404521 1103 88-89 0.33688741703792147 1103 90-91 0.4581621296259115 1103 92-93 0.4984842853312834 1103 94-95 0.4090629519310518 1103 96-97 0.6089835002425872 1103 98-99 0.4770875068393394 1103 100-101 0.9350122095990301 1104 1 0.19670508511620355 1104 2 0.2062437773388659 1104 3 0.20259295522320997 1104 4 0.20611305530303525 1104 5 0.14964466972727308 1104 6 0.030542447440950582 1104 7 0.01944527552320352 1104 8 0.11115135890774042 1104 9 0.15323434633224764 1104 10-11 0.07025085757891247 1104 12-13 0.1861764677395854 1104 14-15 0.2819474455348754 1104 16-17 0.24800743691089622 1104 18-19 0.19738211063490496 1104 20-21 0.19065306478311328 1104 22-23 0.16875746835970773 1104 24-25 0.23929358343980311 1104 26-27 0.18451605265997273 1104 28-29 0.14644791143239644 1104 30-31 0.26668989985682856 1104 32-33 0.1507565393393051 1104 34-35 0.0888706351108155 1104 36-37 0.23527417079183266 1104 38-39 0.3252019457976161 1104 40-41 0.31617083174422334 1104 42-43 0.24607893222579236 1104 44-45 0.22260477878066354 1104 46-47 0.23055760302029427 1104 48-49 0.4675080519874726 1104 50-51 0.4822565475848748 1104 52-53 0.4904105884746457 1104 54-55 0.5202979744644907 1104 56-57 0.27228292463382786 1104 58-59 0.33150676723429484 1104 60-61 0.5646004509974532 1104 62-63 0.3757159312035654 1104 64-65 0.448817893196491 1104 66-67 0.4598946916722966 1104 68-69 0.38768207105978547 1104 70-71 0.4599275115219399 1104 72-73 0.6466588796847539 1104 74-75 0.3847154253744449 1104 76-77 0.32843031591693617 1104 78-79 0.3314322589369034 1104 80-81 0.7267007951169226 1104 82-83 0.6676131282559901 1104 84-85 0.8765261601450014 1104 86-87 0.8711547117577148 1104 88-89 0.7311076125856033 1104 90-91 0.8975131735493989 1104 92-93 0.7368050029757747 1104 94-95 0.8949213140346366 1104 96-97 0.9429444663468161 1104 98-99 0.9945396599709255 1104 100-101 1.5111081356590255 1105 1 -0.06624152867563637 1105 2 -0.03782167455758412 1105 3 -0.010205551159394588 1105 4 0.05408176459761904 1105 5 0.060011344112893994 1105 6 -0.044282549423222406 1105 7 -0.12448473287116002 1105 8 0.027530012143422766 1105 9 0.02511938700148164 1105 10-11 0.019934802639262728 1105 12-13 0.05860515185108284 1105 14-15 0.027339773378109555 1105 16-17 -0.0755688987091716 1105 18-19 -0.10811318080381227 1105 20-21 -0.02089593609491658 1105 22-23 0.00931531949969866 1105 24-25 -0.023146594675033327 1105 26-27 -0.1022839951010468 1105 28-29 -0.036165931062377865 1105 30-31 -0.005277094382904579 1105 32-33 -0.10616175313182907 1105 34-35 0.06843000350748696 1105 36-37 0.001674895408541488 1105 38-39 -0.071620197466153 1105 40-41 0.010211679478231872 1105 42-43 0.03353279942120224 1105 44-45 0.0986122830502083 1105 46-47 -0.007535128138222547 1105 48-49 0.1847590072078944 1105 50-51 0.24253216244772346 1105 52-53 0.23133038614159318 1105 54-55 0.07678635744034068 1105 56-57 -0.11882719346615289 1105 58-59 0.0025750461972506855 1105 60-61 0.06000664883421791 1105 62-63 0.1298343544144629 1105 64-65 0.0546649397321346 1105 66-67 -0.04326658137600958 1105 68-69 0.0746055617632706 1105 70-71 0.1461652818059065 1105 72-73 0.2071638921798069 1105 74-75 0.11859843258201863 1105 76-77 -0.06616231634249559 1105 78-79 0.052162230666237264 1105 80-81 0.11680823329293233 1105 82-83 0.4213371009115967 1105 84-85 0.3719144475988081 1105 86-87 0.5579347987889101 1105 88-89 0.3431339824585251 1105 90-91 0.5075981061532922 1105 92-93 0.23818012237832775 1105 94-95 0.473865879891779 1105 96-97 0.573211445631074 1105 98-99 0.4758787897554697 1105 100-101 0.9444711990219794 1106 1 0.03128192977590416 1106 2 0.06140105517429717 1106 3 0.036629424281706235 1106 4 0.05748806362088743 1106 5 0.035628055605450015 1106 6 -0.028030215212766052 1106 7 -0.05400612034718222 1106 8 0.021656430848914 1106 9 0.012121858404377406 1106 10-11 -0.05603786988870496 1106 12-13 0.08682588962641091 1106 14-15 0.10963105424155373 1106 16-17 -0.012583271434351673 1106 18-19 0.09498555023891697 1106 20-21 0.017535881690264432 1106 22-23 -0.06509381984322005 1106 24-25 -0.08459486682603767 1106 26-27 -0.090859149890953 1106 28-29 -0.23371897314699908 1106 30-31 -0.2548784243253692 1106 32-33 -0.12985162600340772 1106 34-35 0.09140573644472738 1106 36-37 -0.3350463557476573 1106 38-39 0.12562428284115867 1106 40-41 0.03194225778068471 1106 42-43 0.009246406098746718 1106 44-45 0.08538648418304007 1106 46-47 -0.024456517844235748 1106 48-49 -0.10718157974302045 1106 50-51 0.03348216428988593 1106 52-53 -0.18596275627288605 1106 54-55 -0.14848349474087286 1106 56-57 -0.30707405597703996 1106 58-59 -0.14544843746278957 1106 60-61 -0.16087225775664393 1106 62-63 -0.20328741332214406 1106 64-65 -0.22869615123372 1106 66-67 -0.18403219755909817 1106 68-69 -0.33499056407757877 1106 70-71 -0.08936776411816894 1106 72-73 0.11267660405242808 1106 74-75 -0.09567027115795312 1106 76-77 -0.28936688916501296 1106 78-79 -0.17574615193679222 1106 80-81 0.08425751087539979 1106 82-83 -0.06811017114050699 1106 84-85 0.27959060655975065 1106 86-87 0.3921110818532547 1106 88-89 0.15473750505601203 1106 90-91 0.2881533573754176 1106 92-93 0.31922079484424515 1106 94-95 0.3146944809749712 1106 96-97 0.6606296876676119 1106 98-99 0.6367368557009172 1106 100-101 0.9239086349099708 1107 1 0.06966582329515347 1107 2 0.08450754446121422 1107 3 0.022546256694340627 1107 4 0.03288556239665752 1107 5 0.1053115774842226 1107 6 -0.05991187313015445 1107 7 -0.05212946276223107 1107 8 0.0023216517995194863 1107 9 -0.06217365559168542 1107 10-11 -0.003001222946437565 1107 12-13 -0.018332219586667975 1107 14-15 0.07140886899649246 1107 16-17 0.011303625590663557 1107 18-19 -0.13017085893328328 1107 20-21 -0.07224972199317392 1107 22-23 -0.0603187969305381 1107 24-25 -0.003122691529945598 1107 26-27 -0.009972731257931855 1107 28-29 -0.0742974583480489 1107 30-31 -0.07617978037849582 1107 32-33 0.006997397501045555 1107 34-35 0.07505077800615823 1107 36-37 0.059863524651945 1107 38-39 -0.15797055100629365 1107 40-41 -0.09975524507084543 1107 42-43 0.06090113861461788 1107 44-45 0.0804501136837743 1107 46-47 -0.07951626153722913 1107 48-49 -0.09512361291738358 1107 50-51 -0.072628701219692 1107 52-53 -0.07787712615103715 1107 54-55 -0.2933017905472681 1107 56-57 -0.3857906982351693 1107 58-59 -0.39603698945476395 1107 60-61 -0.35923781391828413 1107 62-63 -0.23326692506415014 1107 64-65 -0.18189970035162162 1107 66-67 -0.1286605793209432 1107 68-69 -0.06444715093650188 1107 70-71 -0.13199087911968377 1107 72-73 -0.07366927828381975 1107 74-75 -0.0024123279453327484 1107 76-77 -0.2284252925428767 1107 78-79 -0.12691422069635294 1107 80-81 0.0640991618050677 1107 82-83 0.062166282679999796 1107 84-85 0.27592319515334296 1107 86-87 0.2698385263443335 1107 88-89 0.15289547348069732 1107 90-91 0.11126632701622441 1107 92-93 0.3856871636711219 1107 94-95 0.41990849676611575 1107 96-97 0.6423162556733928 1107 98-99 0.5193890084588482 1107 100-101 0.9252361456232947 1108 1 0.0633623311516871 1108 2 0.16117045342616265 1108 3 0.045854377738521634 1108 4 0.07267546698462013 1108 5 0.16489478748516717 1108 6 0.028427201719487982 1108 7 -0.00520286390214153 1108 8 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-0.4928135642154672 2316 66-67 -0.5076215877358692 2316 68-69 -1.1621959741293217 2316 70-71 -1.1484239677864494 2316 72-73 -0.7379787984619526 2316 74-75 -0.7877506775976819 2316 76-77 -0.8298927534639162 2316 78-79 -0.7632834079255133 2316 80-81 -0.48479335919402544 2316 82-83 -0.8749811485294607 2316 84-85 -0.6368342145888732 2316 86-87 -0.8618784117925564 2316 88-89 -0.9763208905403573 2316 90-91 -1.0821806575120085 2316 92-93 -1.1702072254192544 2316 94-95 -1.2361070906959597 2316 96-97 -1.3100766869230114 2316 98-99 -2.187800971595621 2316 100-101 -2.958902154278139 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 14.0 4 27.0 5 73.0 6 181.0 7 412.0 8 1012.0 9 1780.0 10 3054.0 11 4742.0 12 6720.0 13 9099.0 14 11927.0 15 15627.0 16 19712.0 17 24650.0 18 22338.0 19 16271.0 20 15437.0 21 15855.0 22 17168.0 23 19601.0 24 23237.0 25 27946.0 26 34264.0 27 43094.0 28 53376.0 29 65622.0 30 80016.0 31 99321.0 32 123901.0 33 156184.0 34 203528.0 35 271048.0 36 380608.0 37 479720.0 38 275925.0 39 14364.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.42400799586019 19.284692113271007 14.782882521785842 22.508417369082963 2 15.910613512405813 21.030730792269615 38.75118225669157 24.307473438632996 3 20.448098801628305 24.329112826636088 29.648488144356307 25.574300227379304 4 13.234407544618264 16.915655776397614 36.9275003920623 32.92243628692182 5 13.724129588189424 37.737829681144206 33.80516062578784 14.732880104878529 6 31.976758389542066 36.29133498984579 16.965225059518602 14.76668156109355 7 28.766851783115076 32.73566490218932 20.2399106019161 18.257572712779496 8 26.79377365672365 34.12005853746185 19.206521079554363 19.879646726260138 9 26.116077233513877 17.15183883126821 20.453436790495306 36.278647144722605 10-11 25.47043535070071 26.470607936916977 26.700762295191783 21.358194417190532 12-13 26.74452968339524 23.838408595049398 27.267318219914394 22.149743501640966 14-15 23.03038171749922 25.478626960486565 26.291595847443823 25.19939547457039 16-17 22.22715200996115 28.664760861513244 26.7410534860079 22.36703364251771 18-19 21.999631967884273 27.438074853161492 28.995079245116486 21.567213933837756 20-21 23.117431129036962 25.95085942419249 28.946392672257197 21.98531677451335 22-23 22.528230499894004 26.080300789090643 29.074321731160683 22.317146979854666 24-25 22.90250612523455 26.202173325421164 29.085801355629265 21.809519193715023 26-27 22.517569702033693 26.125251963249568 29.475291889241184 21.88188644547556 28-29 21.95652469446295 27.127036544262705 29.080984188774035 21.835454572500314 30-31 22.367521505603023 26.212385635427477 28.99397791890464 22.426114940064867 32-33 22.24675050110131 26.45227759978137 29.115813561349125 22.185158337768193 34-35 23.02760134619954 26.278296531932444 28.863536331113703 21.830565790754317 36-37 22.79292278933706 25.966847345533754 29.2015717327676 22.038658132361586 38-39 22.369417033679547 26.578739876689976 29.13454442001851 21.917298669611966 40-41 22.589126412215666 26.572725166439703 28.789852536944572 22.04829588440006 42-43 22.312517498458757 26.72633563112766 28.259757219413384 22.7013896510002 44-45 22.706702182573302 26.332693679245654 28.33745759172206 22.623146546458983 46-47 23.22637233429077 26.894664848071592 27.891470617034493 21.98749220060315 48-49 23.138972971567227 26.836616563810413 27.72195397199042 22.302456492631944 50-51 22.46540834904375 27.138894016788555 28.08173580925185 22.31396182491584 52-53 23.1258847275704 27.444094083296637 27.154854045193634 22.275167143939324 54-55 22.62746973975281 26.88744819368449 27.568328382886403 22.91675368367629 56-57 23.070637304424295 27.006890507163316 27.351465126869158 22.57100706154323 58-59 23.65655243902199 26.76442592873598 27.188734759191778 22.39028687305025 60-61 23.00205906353499 27.007389888183138 28.0204475709297 21.970103477352172 62-63 22.687750789561008 27.116839265320085 27.86594684660329 22.329463098515618 64-65 22.4456184393837 27.538065614537743 27.475895554298795 22.540420391779758 66-67 22.792744140703885 27.15164630867842 27.105129338259154 22.95048021235854 68-69 22.672358390235463 27.029809243068016 27.50714486391364 22.79068750278288 70-71 23.115748967091744 26.950694847644186 27.299611069726698 22.633945115537372 72-73 22.9817066491401 26.745209156368627 27.7173266316293 22.55575756286197 74-75 22.528149646517555 27.83638559149475 27.079712690475944 22.555752071511755 76-77 22.819746766211697 27.156472915853136 27.092796041315886 22.930984276619277 78-79 22.858207398075344 27.253421625116026 27.161328419856744 22.727042556951886 80-81 23.237143930207345 27.302372176259794 26.893518279507177 22.566965614025687 82-83 23.065448626749323 27.16478614019808 27.32132371197402 22.44844152107858 84-85 22.75152003418057 27.400483383876324 27.131338993833147 22.716657588109953 86-87 22.801982035732287 27.43631464935472 26.937554603910087 22.824148711002906 88-89 22.684196677497273 27.51368115444996 26.60280649678679 23.19931567126598 90-91 22.90501537955116 27.051590731467417 26.895518592946004 23.147875296035416 92-93 23.20123716492629 27.20383305131743 27.01822678019827 22.576703003558013 94-95 22.79415184578771 27.032439731753982 27.208745192296323 22.96466323016199 96-97 22.362077978602365 27.077040317093115 27.650478486064888 22.910403218239637 98-99 22.467724191032495 27.39348978112593 26.74076867411787 23.398017353723706 100-101 22.291439260767284 27.183180417343255 26.906222456245903 23.61915786564355 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 290.0 1 239.0 2 264.0 3 354.0 4 300.0 5 265.0 6 441.5 7 865.0 8 1375.5 9 1737.0 10 1789.0 11 1742.5 12 1849.5 13 2071.0 14 2336.0 15 2559.0 16 2809.0 17 3065.0 18 3328.0 19 3652.5 20 4083.0 21 4599.0 22 5285.0 23 6304.5 24 7550.5 25 9343.5 26 11863.0 27 15028.5 28 18143.0 29 21985.0 30 27359.5 31 32929.5 32 39038.0 33 44398.0 34 49202.0 35 53814.0 36 59139.5 37 64497.5 38 67439.0 39 69365.0 40 71435.5 41 75027.5 42 78642.5 43 82238.0 44 86396.0 45 90098.5 46 91579.5 47 100610.0 48 122498.5 49 121853.5 50 100968.0 51 90220.0 52 85876.0 53 83921.5 54 82039.0 55 77433.5 56 70781.0 57 64157.5 58 59122.5 59 53047.5 60 44753.5 61 36914.0 62 30346.0 63 23801.0 64 17604.0 65 13363.0 66 10152.5 67 8012.0 68 5963.0 69 4113.0 70 3018.0 71 2242.0 72 1743.0 73 1470.5 74 1199.0 75 916.5 76 641.5 77 385.5 78 234.0 79 158.5 80 110.5 81 74.5 82 50.0 83 37.0 84 19.5 85 8.0 86 8.0 87 6.0 88 5.5 89 4.5 90 4.0 91 2.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021632381902562076 2 0.013475910037661623 3 0.009929617922487512 4 0.0 5 0.0027188239549668187 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0011426941260005469 14-15 1.3791136003454875E-4 16-17 7.880649144831358E-5 18-19 0.0015761298289662716 20-21 0.0 22-23 1.5761298289662716E-4 24-25 0.0 26-27 0.0016352346975525068 28-29 0.0030143482978979945 30-31 0.0013791136003454877 32-33 0.007939754013417594 34-35 0.0020095655319319963 36-37 0.0010244843888280765 38-39 0.0012215006174488604 40-41 2.3641947434494077E-4 42-43 0.005457349532795715 44-45 0.0012609038631730173 46-47 2.1671785148286236E-4 48-49 6.304519315865086E-4 50-51 9.850811431039198E-5 52-53 1.7731460575870557E-4 54-55 0.0017337428118628989 56-57 0.015189951226662442 58-59 0.026675997355254145 60-61 0.021415664051079215 62-63 0.014815620392282952 64-65 0.01840131575318122 66-67 0.0030931547893463082 68-69 9.850811431039198E-4 70-71 1.3791136003454875E-4 72-73 0.0 74-75 0.0016352346975525068 76-77 0.0019307590404836826 78-79 0.008491399453555789 80-81 0.015879508026835187 82-83 0.01781026706731887 84-85 0.029473627801669282 86-87 0.010599473099798177 88-89 0.04214177130198569 90-91 0.047855241931988424 92-93 0.030379902453324885 94-95 0.03104975763063555 96-97 0.05201228435588696 98-99 0.05530245537385405 100-101 0.06133115196965004 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2537862.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.112510077509725 #Duplication Level Percentage of deduplicated Percentage of total 1 78.06271760509013 44.58357745898506 2 13.008809078244715 14.859314791553027 3 3.9719048313434877 6.805363641210435 4 1.6828938499299597 3.844571678540159 5 0.8755680901251203 2.500294568540844 6 0.515203057021644 1.765472387166748 7 0.3364183826619388 1.3449588789027653 8 0.23897052712849193 1.0918565311083046 9 0.1733525489354329 0.8910539278233219 >10 1.0103028748625242 10.594674865616026 >50 0.0801674575081376 3.149465043557558 >100 0.03961370441076422 4.122343273249971 >500 0.002350029713250992 0.89419499980931 >1k 0.0013823704195534679 1.8783937343627104 >5k 2.764740839106935E-4 1.098598218462387 >10k+ 6.911852097767338E-5 0.5758660011113654 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 14588 0.5748145486239993 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8334 0.32838664986512267 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7854 0.3094730919175275 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6591 0.2597067925679174 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5051 0.19902579415271596 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4343 0.17112829618001296 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4319 0.17018261828263317 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4063 0.16009538737724904 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3782 0.14902307532876097 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3702 0.14587081567082844 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3057 0.12045572217874731 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2813 0.11084133022205304 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2802 0.11040789451908734 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.940324572415679E-5 0.0 0.0 0.0 0.0 3 3.940324572415679E-5 0.0 0.0 0.0 0.0 4 3.940324572415679E-5 0.0 0.0 0.0 0.0 5 3.940324572415679E-5 0.0 0.0 0.0 0.0 6 3.940324572415679E-5 0.0 0.0 0.0 0.0 7 3.940324572415679E-5 0.0 0.0 0.0 0.0 8 3.940324572415679E-5 0.0 0.0 0.0 0.0 9 3.940324572415679E-5 0.0 0.0 0.0 0.0 10-11 3.940324572415679E-5 0.0 0.0 0.0 0.0 12-13 3.940324572415679E-5 0.0 0.0 0.0 0.0 14-15 7.880649144831358E-5 0.0 0.0 0.0 0.0 16-17 9.850811431039198E-5 0.0 0.0 0.0 3.940324572415679E-5 18-19 1.1820973717247037E-4 0.0 0.0 0.0 3.940324572415679E-5 20-21 1.1820973717247037E-4 0.0 0.0 0.0 3.940324572415679E-5 22-23 1.1820973717247037E-4 0.0 0.0 0.0 5.9104868586235185E-5 24-25 1.1820973717247037E-4 0.0 0.0 0.0 7.880649144831358E-5 26-27 1.1820973717247037E-4 0.0 0.0 5.9104868586235185E-5 7.880649144831358E-5 28-29 1.1820973717247037E-4 0.0 0.0 3.743308343794895E-4 7.880649144831358E-5 30-31 1.1820973717247037E-4 0.0 0.0 0.0011623957488626252 7.880649144831358E-5 32-33 1.1820973717247037E-4 0.0 0.0 0.002383896366311486 7.880649144831358E-5 34-35 1.1820973717247037E-4 0.0 0.0 0.0033295742636912486 9.850811431039198E-5 36-37 1.1820973717247037E-4 1.9701622862078395E-5 0.0 0.00490570409265752 1.1820973717247037E-4 38-39 1.5761298289662716E-4 3.940324572415679E-5 0.0 0.005989293350071832 1.1820973717247037E-4 40-41 1.5761298289662716E-4 3.940324572415679E-5 0.0 0.007663931293348495 1.1820973717247037E-4 42-43 1.5761298289662716E-4 3.940324572415679E-5 0.0 0.011111715294212215 1.1820973717247037E-4 44-45 1.9701622862078395E-4 9.850811431039198E-5 0.0 0.015879508026835187 1.1820973717247037E-4 46-47 1.9701622862078395E-4 1.1820973717247037E-4 0.0 0.027365554155426894 1.1820973717247037E-4 48-49 1.9701622862078395E-4 1.1820973717247037E-4 0.0 0.04862360522360948 1.1820973717247037E-4 50-51 1.9701622862078395E-4 1.1820973717247037E-4 0.0 0.06440460513613427 1.1820973717247037E-4 52-53 1.9701622862078395E-4 1.1820973717247037E-4 0.0 0.08040232290014193 1.1820973717247037E-4 54-55 1.9701622862078395E-4 1.1820973717247037E-4 0.0 0.10402456871177393 1.1820973717247037E-4 56-57 2.3641947434494074E-4 1.1820973717247037E-4 0.0 0.12386410293388687 1.1820973717247037E-4 58-59 2.3641947434494074E-4 1.1820973717247037E-4 0.0 0.148274413660002 1.1820973717247037E-4 60-61 2.3641947434494074E-4 1.3791136003454878E-4 0.0 0.18627884416095122 1.1820973717247037E-4 62-63 2.3641947434494074E-4 1.5761298289662716E-4 0.0 0.21632381902562078 1.1820973717247037E-4 64-65 2.3641947434494074E-4 1.5761298289662716E-4 0.0 0.25007269898836104 1.3791136003454878E-4 66-67 3.152259657932543E-4 1.9701622862078395E-4 0.0 0.31195549639814935 1.5761298289662716E-4 68-69 3.152259657932543E-4 1.9701622862078395E-4 0.0 0.3852849366908051 1.5761298289662716E-4 70-71 3.152259657932543E-4 1.9701622862078395E-4 0.0 0.4441139825569712 1.5761298289662716E-4 72-73 3.152259657932543E-4 2.3641947434494074E-4 0.0 0.5105478548478996 1.5761298289662716E-4 74-75 3.152259657932543E-4 2.3641947434494074E-4 0.0 0.5687858520282032 1.5761298289662716E-4 76-77 3.152259657932543E-4 2.3641947434494074E-4 0.0 0.6193599179151585 1.5761298289662716E-4 78-79 3.152259657932543E-4 2.7582272006909756E-4 0.0 0.6899114293842612 1.5761298289662716E-4 80-81 4.728389486898815E-4 2.9552434293117594E-4 0.0 0.8049098020302128 1.5761298289662716E-4 82-83 4.728389486898815E-4 3.3492758865533276E-4 0.0 0.9184896578300947 1.5761298289662716E-4 84-85 4.925405715519599E-4 3.5462921151741114E-4 0.0 1.0521454673264348 1.5761298289662716E-4 86-87 5.319438172761167E-4 3.5462921151741114E-4 0.0 1.2839941651673732 1.9701622862078395E-4 88-89 5.516454401381951E-4 3.5462921151741114E-4 0.0 1.5682294781985782 2.3641947434494074E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11285 0.0 23.240221 1 TACATGG 11490 0.0 22.572578 2 GAGTACT 10110 0.0 21.93758 12-13 ACATGGG 11375 0.0 21.8373 3 CATGGGG 5690 0.0 20.615343 4 GTACTTT 10695 0.0 20.514385 14-15 TACCTGG 2065 0.0 20.472858 2 AGTACTT 10400 0.0 20.252716 12-13 GTACCTG 2275 0.0 19.422462 1 AGAGTAC 16640 0.0 19.250547 10-11 GTGTAGC 1315 0.0 19.149298 1 ACTTTTT 12255 0.0 18.213017 16-17 ATTCGCG 330 0.0 17.99659 80-81 TACTTTT 11640 0.0 17.767752 14-15 TAAGGTG 1135 0.0 17.155125 5 GAGTACA 9260 0.0 16.88059 1 GTATAGG 745 0.0 16.581347 1 CATGGGA 5950 0.0 16.442057 4 AGTACAT 8810 0.0 16.067522 2 TCGCGCT 400 0.0 16.033382 60-61 >>END_MODULE