FastQCFastQC Report
Thu 26 May 2016
SRR936583_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936583_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792778
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT224030.8021761844299834No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT151010.5407160898574824No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT116540.4172905973908417No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC103230.36963195785701547No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT70220.25143423501617385No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT68610.24566936577128579No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC55380.19829717936764038No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55000.1969365269992817No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC53710.19231747027511675No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC52950.18959616553839942No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG47370.16961605970829047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43800.15683308877397342No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA42120.1508175730401772No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA35510.1271493831589908No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT31290.11203898054195499No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA31240.11185994733559201No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC30420.1089238027512391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG116650.028.2242771
TACATGG120000.026.803382
ACATGGG118950.026.4798583
CATGGGG60850.022.1737884
CATGGGA59500.021.2396244
GAGTACT71550.020.11922512-13
TACCTGG21550.019.8416292
GTACTTT74300.019.37526314-15
GTACCTG22650.019.2971861
AGTACTT75550.018.29939712-13
ATACCGA1851.5197405E-417.976966
ATGGGGA36000.017.4205285
ATGGGAG28800.017.3215485
AGAGTAC149600.017.05455610-11
AGTACAT88050.016.7808192
TAGTACG2002.7578144E-416.628394
CGTTCGG1306.655375E-616.43623734-35
ACTTTTT89250.016.18358416-17
ACCGTCC2657.921295E-616.1370988
GTGTAGC12750.016.0225871
Produced by FastQC (version 0.11.5)