##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936583_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2792778 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.537721938514267 31.0 31.0 34.0 30.0 34.0 2 31.619429829367032 33.0 31.0 34.0 30.0 34.0 3 31.64593676976831 33.0 31.0 34.0 30.0 34.0 4 35.275485197892564 37.0 35.0 37.0 33.0 37.0 5 35.28695907802196 37.0 35.0 37.0 33.0 37.0 6 35.29317797547818 37.0 35.0 37.0 33.0 37.0 7 35.20730577224541 37.0 35.0 37.0 32.0 37.0 8 35.17155534739961 37.0 35.0 37.0 32.0 37.0 9 36.68574050640617 39.0 37.0 39.0 32.0 39.0 10-11 36.558459712873706 39.0 37.0 39.0 32.0 39.0 12-13 36.47316542883108 39.0 37.0 39.0 32.0 39.0 14-15 37.58217176589045 40.0 37.0 41.0 32.0 41.0 16-17 37.491910742636904 40.0 37.0 41.0 32.0 41.0 18-19 37.44566646543334 40.0 37.0 41.0 32.0 41.0 20-21 37.29170578542225 39.5 37.0 41.0 31.5 41.0 22-23 37.41698176510987 40.0 37.0 41.0 32.0 41.0 24-25 37.44610097902519 40.0 37.0 41.0 32.0 41.0 26-27 37.35791781516468 40.0 37.0 41.0 31.5 41.0 28-29 37.23460762008294 39.0 37.0 41.0 31.0 41.0 30-31 37.06349913956641 39.0 36.0 41.0 31.0 41.0 32-33 36.89146022347641 39.0 36.0 41.0 30.0 41.0 34-35 36.692451924213096 39.0 36.0 41.0 30.0 41.0 36-37 36.487692899328195 39.0 35.0 40.0 30.0 41.0 38-39 36.2959866484196 39.0 35.0 40.0 30.0 41.0 40-41 36.12625350099435 39.0 35.0 40.0 29.5 41.0 42-43 35.90625230505253 38.0 35.0 40.0 29.0 41.0 44-45 35.66631415028334 38.0 35.0 40.0 27.5 41.0 46-47 35.535279746546266 38.0 35.0 40.0 27.5 41.0 48-49 35.39597973773783 38.0 35.0 40.0 27.0 41.0 50-51 34.42292047559813 37.5 33.5 39.5 25.0 40.5 52-53 34.549647877489726 38.0 33.5 39.5 26.0 40.5 54-55 35.00377903291991 38.0 34.0 40.0 26.0 41.0 56-57 34.870318192137006 38.0 34.0 40.0 26.0 41.0 58-59 34.622113179064 38.0 34.0 40.0 26.0 41.0 60-61 34.28466834814654 37.5 33.5 40.0 25.0 41.0 62-63 33.93664158053379 37.0 33.0 40.0 24.0 41.0 64-65 33.57542006561209 36.5 33.0 39.5 23.0 41.0 66-67 33.07528453747487 36.0 32.0 39.0 22.0 41.0 68-69 32.66713430140169 35.0 32.0 39.0 21.5 40.0 70-71 32.22213115399792 35.0 31.0 38.0 20.5 40.0 72-73 31.81391843533571 35.0 31.0 37.0 20.0 39.5 74-75 31.39277701270921 35.0 31.0 37.0 18.5 39.0 76-77 30.93377669116557 34.5 30.5 36.0 17.5 39.0 78-79 30.429742356893385 34.0 30.0 36.0 12.0 37.5 80-81 29.9351505919912 34.0 29.0 35.0 7.5 37.0 82-83 29.51359345425952 34.0 29.0 35.0 7.0 37.0 84-85 29.14914074802938 34.0 29.0 35.0 2.0 36.0 86-87 28.82415054114577 34.0 29.0 35.0 2.0 36.0 88-89 28.40438069191321 34.0 28.5 35.0 2.0 35.5 90-91 27.967034436679178 33.5 27.0 35.0 2.0 35.0 92-93 27.480485738572845 33.0 26.5 35.0 2.0 35.0 94-95 26.953042633535496 33.0 25.0 35.0 2.0 35.0 96-97 26.33885579161681 33.0 25.0 35.0 2.0 35.0 98-99 25.55567234488384 32.5 22.0 35.0 2.0 35.0 100-101 22.628675462210026 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4398029014246063 1101 2 0.4205320460117754 1101 3 0.3623143821920074 1101 4 0.4377059954915339 1101 5 0.32061513153811205 1101 6 0.4057151357804756 1101 7 0.5703705417786793 1101 8 0.45363356244628505 1101 9 0.5085776261117445 1101 10-11 0.75011180289534 1101 12-13 0.645138288834481 1101 14-15 0.6184154487975917 1101 16-17 0.7146048303410666 1101 18-19 0.6797236767894788 1101 20-21 0.74355896079053 1101 22-23 0.6542503564218478 1101 24-25 0.7475085385994262 1101 26-27 0.620517153199998 1101 28-29 0.664958918243606 1101 30-31 0.6718536875552488 1101 32-33 0.5217378493659623 1101 34-35 0.5281132933865678 1101 36-37 0.42269725604708697 1101 38-39 0.41433298700709287 1101 40-41 0.41901840344190333 1101 42-43 0.4084837512355932 1101 44-45 0.47026440761091237 1101 46-47 0.5018263843435875 1101 48-49 0.5552127067966524 1101 50-51 0.5018427946865884 1101 52-53 0.6067795537322453 1101 54-55 0.5309501194072794 1101 56-57 0.47009971608554935 1101 58-59 0.4732652071947143 1101 60-61 0.5358985521947659 1101 62-63 0.5559961836639147 1101 64-65 0.33044592639465975 1101 66-67 0.4511417520225933 1101 68-69 0.49822395380206785 1101 70-71 0.4186998119980174 1101 72-73 0.3442339922032325 1101 74-75 0.30846991219478426 1101 76-77 0.44907730159893333 1101 78-79 0.41111439537731087 1101 80-81 0.45090132602092936 1101 82-83 0.41193923309032243 1101 84-85 0.3736187916304381 1101 86-87 0.2506592810625641 1101 88-89 0.22025382951322214 1101 90-91 0.40359153936981684 1101 92-93 0.2555726909671989 1101 94-95 0.24322582082873723 1101 96-97 0.144546385208848 1101 98-99 0.26571118505843927 1101 100-101 0.3281506061060071 1102 1 0.2150561085215088 1102 2 0.3262223699031672 1102 3 0.3084117848816881 1102 4 0.25406446610645617 1102 5 0.35196580974838554 1102 6 0.3570801092768221 1102 7 0.3545730400851923 1102 8 0.4710608109668897 1102 9 0.49244490101230554 1102 10-11 0.5017802880529345 1102 12-13 0.4670177610457742 1102 14-15 0.48867322712276007 1102 16-17 0.3576924331805458 1102 18-19 0.36514027437453933 1102 20-21 0.40199425856775406 1102 22-23 0.1985179782831139 1102 24-25 0.2549751412636354 1102 26-27 0.04446370483066886 1102 28-29 -0.06319590033626099 1102 30-31 0.3417096872857641 1102 32-33 0.21801591689171573 1102 34-35 0.24328774482602 1102 36-37 0.06374885960870103 1102 38-39 -0.0016053622031577675 1102 40-41 -0.016242267099649155 1102 42-43 0.07269887634303984 1102 44-45 0.15234811398489967 1102 46-47 0.13860782384099934 1102 48-49 0.3123502178694437 1102 50-51 0.22677920013796182 1102 52-53 0.38850274625490044 1102 54-55 0.34487362804735255 1102 56-57 0.26855394616912776 1102 58-59 0.27547681848294303 1102 60-61 0.28958474645381216 1102 62-63 0.41967357758484525 1102 64-65 0.42376451179293895 1102 66-67 0.3946537922220372 1102 68-69 0.32406094648652584 1102 70-71 0.37748164940545337 1102 72-73 0.1286325506741619 1102 74-75 0.1705584292799145 1102 76-77 0.19513570279013237 1102 78-79 0.14947894833118625 1102 80-81 0.26872130015678053 1102 82-83 0.34188009397603025 1102 84-85 0.01801749747545145 1102 86-87 0.08373970994219704 1102 88-89 -0.023721070575046355 1102 90-91 -5.905686795983911E-4 1102 92-93 0.07418190595282681 1102 94-95 -0.0560281768248565 1102 96-97 0.026606590679900677 1102 98-99 0.08568953159491244 1102 100-101 0.19230091293358242 1103 1 0.23697125694151921 1103 2 0.1329251662441635 1103 3 0.21296803678937692 1103 4 0.1999342226709544 1103 5 0.296949597571448 1103 6 0.18743570373781182 1103 7 0.36363819574612677 1103 8 0.27696551177884743 1103 9 0.07562736379347967 1103 10-11 0.27448890142828475 1103 12-13 -0.06038248498198584 1103 14-15 0.1973948071382594 1103 16-17 0.38724397913311037 1103 18-19 0.4917110403524134 1103 20-21 0.4308842570713267 1103 22-23 0.2980421017628956 1103 24-25 0.22837828900271262 1103 26-27 0.12382618122874334 1103 28-29 0.293034736865998 1103 30-31 0.2657397630126681 1103 32-33 0.1925367343572617 1103 34-35 0.3912288022319501 1103 36-37 0.533321079888232 1103 38-39 0.47820796407963684 1103 40-41 0.37089324967625004 1103 42-43 0.3029988436145743 1103 44-45 0.501131625662083 1103 46-47 0.6980204965193977 1103 48-49 0.5460812033339195 1103 50-51 0.4838865277042217 1103 52-53 0.5250823572746484 1103 54-55 0.614232893627161 1103 56-57 0.5233207448782196 1103 58-59 0.4454890854110971 1103 60-61 0.38697088021596215 1103 62-63 0.4585085407508842 1103 64-65 0.4755944585185077 1103 66-67 0.5853987959622415 1103 68-69 0.42656181811126004 1103 70-71 0.3925621572057949 1103 72-73 0.33082976004492437 1103 74-75 0.16015712696444595 1103 76-77 0.27742832007050566 1103 78-79 0.27808919126357523 1103 80-81 0.3092983066834343 1103 82-83 0.14303636761080085 1103 84-85 0.2593981045294491 1103 86-87 0.3543311388752137 1103 88-89 0.1210655538814649 1103 90-91 0.2628394518939352 1103 92-93 0.18792921343575486 1103 94-95 0.11345082031435183 1103 96-97 0.0424780866170984 1103 98-99 -0.0668120593840591 1103 100-101 0.05558044632337911 1104 1 0.13312183667498445 1104 2 0.18913997768179414 1104 3 0.15651252952780936 1104 4 0.1777838167141894 1104 5 0.19060908202975213 1104 6 0.2573371613409563 1104 7 0.3047720914071448 1104 8 0.3934924662941697 1104 9 0.4048955233481877 1104 10-11 0.40032835252110033 1104 12-13 0.2756743707087068 1104 14-15 0.29575393081019996 1104 16-17 0.20390170106251304 1104 18-19 0.2470502586039771 1104 20-21 0.0524498041266952 1104 22-23 0.2334794408386145 1104 24-25 0.25131654777255363 1104 26-27 0.13118413363859105 1104 28-29 0.027686228927159107 1104 30-31 0.29245850825610376 1104 32-33 0.14014798362094893 1104 34-35 0.2521348391796465 1104 36-37 0.095741707733346 1104 38-39 0.04190396028388221 1104 40-41 -0.08864424690789718 1104 42-43 0.1542859671956336 1104 44-45 0.23506591161955015 1104 46-47 0.04709968855179625 1104 48-49 0.15743683647266238 1104 50-51 0.05778280821710524 1104 52-53 0.1274804580496962 1104 54-55 0.07384365510905155 1104 56-57 0.06571271998004846 1104 58-59 0.029900498475740278 1104 60-61 0.007891199159232087 1104 62-63 0.06160720660198393 1104 64-65 0.05641819914994528 1104 66-67 0.10435345896900827 1104 68-69 0.036347514513416 1104 70-71 -0.025874708022207926 1104 72-73 -0.2605357387825258 1104 74-75 -0.12140635394093025 1104 76-77 -0.20571835714228115 1104 78-79 -0.07220386388976152 1104 80-81 -0.030268607687521154 1104 82-83 -0.13103292633835295 1104 84-85 -0.24248459477481532 1104 86-87 -0.25882057147185833 1104 88-89 -0.26507986313957943 1104 90-91 -0.360858010495793 1104 92-93 -0.5689693800468341 1104 94-95 -0.5281777265653709 1104 96-97 -0.5916707399064443 1104 98-99 -0.6161487125210527 1104 100-101 -0.6137235051973562 1105 1 0.2557933118297093 1105 2 0.14808333034804733 1105 3 0.24133123860745798 1105 4 0.21343347450614658 1105 5 0.13886963109833772 1105 6 0.317692602116189 1105 7 0.3695478261178451 1105 8 0.399058146179911 1105 9 0.3688440206530075 1105 10-11 0.42359600426234323 1105 12-13 0.26737320203111636 1105 14-15 0.4151415396538667 1105 16-17 0.35832948159888645 1105 18-19 0.27040941324515444 1105 20-21 0.22247530976019902 1105 22-23 0.0744003695354607 1105 24-25 0.13595378676533443 1105 26-27 -0.10645017726138661 1105 28-29 -0.08254533099891859 1105 30-31 -0.1296600462284303 1105 32-33 -0.15942451227101628 1105 34-35 -0.0996698155664717 1105 36-37 -0.048573082718611715 1105 38-39 -0.14638789173481115 1105 40-41 -0.08459988271868468 1105 42-43 -0.07940636038218685 1105 44-45 0.07019016359933516 1105 46-47 0.18131748592982433 1105 48-49 0.12753106526318447 1105 50-51 0.20796402235180267 1105 52-53 0.17306329952828037 1105 54-55 0.19580678263977802 1105 56-57 0.03892476611286355 1105 58-59 0.21316736956288196 1105 60-61 0.2864762571835868 1105 62-63 0.24982739201372794 1105 64-65 0.30184305415303925 1105 66-67 0.43325327417154114 1105 68-69 0.37680836918889327 1105 70-71 0.13543146707979048 1105 72-73 0.05407330707898339 1105 74-75 0.18127148162515994 1105 76-77 0.1425467990653253 1105 78-79 0.19734480285539746 1105 80-81 0.23497206413573224 1105 82-83 -0.04969740632033037 1105 84-85 0.08302385420623892 1105 86-87 -0.10852641844317645 1105 88-89 -0.15107236886011322 1105 90-91 -0.17275300355948886 1105 92-93 -0.21362138747968729 1105 94-95 0.05205527949045674 1105 96-97 0.05886464804025948 1105 98-99 0.21271238991612407 1105 100-101 0.2542790824790586 1106 1 0.26582739004158285 1106 2 0.18983462259176065 1106 3 0.25028922145089183 1106 4 0.2055687785374687 1106 5 0.16314533899810613 1106 6 0.293056987708475 1106 7 0.384689082685 1106 8 0.4316084513152134 1106 9 0.34935953910051865 1106 10-11 0.37939854652370997 1106 12-13 0.3747769054346932 1106 14-15 0.38466834019001084 1106 16-17 0.37065844005008586 1106 18-19 0.3223450932221965 1106 20-21 0.042198065590262956 1106 22-23 0.32899891162137607 1106 24-25 0.2881575334357578 1106 26-27 0.22744874114196278 1106 28-29 0.2783738031290284 1106 30-31 0.3937917238833961 1106 32-33 0.2889910720431885 1106 34-35 0.3834967725458469 1106 36-37 0.2421706014007512 1106 38-39 0.31762749772993715 1106 40-41 0.22128510443136662 1106 42-43 0.23659438786651066 1106 44-45 0.26311663933682894 1106 46-47 0.3501876545589795 1106 48-49 0.36318410275144686 1106 50-51 0.076916607991528 1106 52-53 0.0885405152997123 1106 54-55 0.20271656012779005 1106 56-57 0.3431854346970553 1106 58-59 0.3407580831014556 1106 60-61 0.326327609197989 1106 62-63 0.4419305804527198 1106 64-65 0.38938108919917624 1106 66-67 0.44493810869818873 1106 68-69 0.440802264624395 1106 70-71 0.3237942483793006 1106 72-73 0.25827155655617773 1106 74-75 0.47436840417826076 1106 76-77 0.3145490278279581 1106 78-79 0.10882578328793358 1106 80-81 0.35510705370188234 1106 82-83 0.09347508451891784 1106 84-85 0.23821260393582477 1106 86-87 0.36231989989817137 1106 88-89 0.396035146262836 1106 90-91 0.272961144622748 1106 92-93 0.1649341877351027 1106 94-95 0.24430580378493616 1106 96-97 0.3630606858533909 1106 98-99 0.46681906906723114 1106 100-101 0.34765855905437704 1107 1 0.16448117936309714 1107 2 0.1602685811608815 1107 3 0.11744333539323293 1107 4 0.23366618990083765 1107 5 0.2031289627327979 1107 6 0.2541148538036566 1107 7 0.32430980017515765 1107 8 0.3167792369551279 1107 9 0.40434560096625916 1107 10-11 0.43924912796069293 1107 12-13 0.3350105902544698 1107 14-15 0.11816681332661716 1107 16-17 0.1516860899994512 1107 18-19 0.20005386544228543 1107 20-21 0.2887033711493743 1107 22-23 0.25462019409460623 1107 24-25 0.25021825885906424 1107 26-27 0.2805964512398944 1107 28-29 0.37001493899614246 1107 30-31 0.3285448543679337 1107 32-33 0.09052696519525227 1107 34-35 0.18678723816417175 1107 36-37 0.2238521708458805 1107 38-39 0.21394989313545665 1107 40-41 0.16498665993692185 1107 42-43 0.3252684998993729 1107 44-45 0.29882564947479295 1107 46-47 0.305861789885185 1107 48-49 0.3749634342060446 1107 50-51 0.3647255405713281 1107 52-53 0.485533911976205 1107 54-55 0.585232385453125 1107 56-57 0.380872652439173 1107 58-59 0.5219162044551027 1107 60-61 0.4617765488290573 1107 62-63 0.6244940181110934 1107 64-65 0.47912199739556627 1107 66-67 0.7014712886086727 1107 68-69 0.7613297943207442 1107 70-71 0.521188539558004 1107 72-73 0.4192990167080488 1107 74-75 0.553079057981769 1107 76-77 0.48567151038695755 1107 78-79 0.3983634547850734 1107 80-81 0.6339015634927954 1107 82-83 0.6497804307407158 1107 84-85 0.28194923363638935 1107 86-87 0.3921657009303843 1107 88-89 0.23429671233570915 1107 90-91 0.273452940625706 1107 92-93 0.42779860013785154 1107 94-95 0.4644722005972568 1107 96-97 0.34264724554456194 1107 98-99 0.37322129349395894 1107 100-101 0.3178852261594294 1108 1 0.1955041819499712 1108 2 0.18927650887917125 1108 3 0.17969800922810464 1108 4 0.31937908032283246 1108 5 0.28427362074047835 1108 6 0.1174506063540548 1108 7 0.18663950720256395 1108 8 0.12342962057028473 1108 9 0.2157253434166151 1108 10-11 0.3141192446490422 1108 12-13 0.12223812870923467 1108 14-15 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2316 58-59 -2.224696045355884 2316 60-61 -2.476373227923915 2316 62-63 -2.454086228570297 2316 64-65 -2.325387028404794 2316 66-67 -2.237357595457091 2316 68-69 -2.3307111763054316 2316 70-71 -2.138774898333434 2316 72-73 -2.113354783005324 2316 74-75 -2.0410418151454586 2316 76-77 -2.2441526379077494 2316 78-79 -2.905783861924789 2316 80-81 -2.974433694492003 2316 82-83 -2.8270782371497027 2316 84-85 -2.5536256979977345 2316 86-87 -2.4553544326792967 2316 88-89 -2.803295304184946 2316 90-91 -2.8015331605068887 2316 92-93 -3.5252356411043557 2316 94-95 -3.474542127084998 2316 96-97 -3.7980743671002095 2316 98-99 -5.063614880424318 2316 100-101 -5.694146265401567 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4910.0 3 1961.0 4 2036.0 5 2266.0 6 2652.0 7 3100.0 8 3855.0 9 4815.0 10 6121.0 11 7569.0 12 9573.0 13 11552.0 14 13035.0 15 15483.0 16 18677.0 17 22916.0 18 19373.0 19 16431.0 20 16962.0 21 18829.0 22 21127.0 23 24247.0 24 27921.0 25 32386.0 26 38300.0 27 45077.0 28 53751.0 29 65622.0 30 80372.0 31 99137.0 32 124977.0 33 160979.0 34 213311.0 35 291528.0 36 404354.0 37 505779.0 38 357955.0 39 43832.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.44488630971672 17.689345038684596 13.559000950776287 23.306767700822395 2 15.48275066822897 19.86509366665043 39.183464305256734 25.46869135986386 3 20.763814603669378 23.196959696714565 29.326521396112888 26.712704303503177 4 12.618518185591554 15.427965474969854 37.25214366830415 34.701372671134436 5 13.623234916559692 37.486496825142126 34.14161356134238 14.748654696955803 6 32.806243260767545 35.14538830517036 17.01671866995762 15.031649764104474 7 29.346357156310944 32.2158427229425 20.15701350628405 18.280786614462517 8 26.852572147343327 32.87263472470545 19.49783386054188 20.776959267409342 9 25.95137973522838 17.191601237893288 20.599676199209124 36.257342827669206 10-11 25.528418211884528 25.368515901306885 27.056624366941417 22.046441519867162 12-13 27.13156693461777 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28.529194501944126 22.835472608010186 38-39 22.745383579883168 26.179870168583193 28.31509371233026 22.759652539203383 40-41 22.780802680088115 26.382511250583658 28.13428298476337 22.70240308456486 42-43 22.39234168779931 26.464239701104102 27.2587996181443 23.884618992952284 44-45 22.85090832007872 25.859693963415115 27.516634213449308 23.772763503056858 46-47 23.651681832683614 26.540905635542227 27.214397815492003 22.593014716282152 48-49 23.359005685419216 26.43392302519588 27.09242977730258 23.114641512082322 50-51 22.27441796760653 26.715794882537192 28.101590132103695 22.90819701775258 52-53 23.288716039970975 27.00946565060436 26.70670949535329 22.995108814071376 54-55 22.3777311793997 26.16625451451628 27.450407461278825 24.0056068448052 56-57 23.213613056617827 26.290658703218323 26.975644165628264 23.520084074535585 58-59 24.034559450440568 25.964133541165573 26.742215332676604 23.259091675717254 60-61 23.051119595116706 26.15289615161782 28.225491691570824 22.570492561694643 62-63 22.67723922349356 26.471597144765134 27.889278188461976 22.961885443279332 64-65 22.34694142448561 27.324360736009595 27.243294050245947 23.085403789258844 66-67 22.633395282359892 26.684123889219453 26.630441497384194 24.05203933103646 68-69 22.425959421434037 26.48845477595963 27.38780071107325 23.697785091533085 70-71 23.24469006441046 26.384679772063745 26.896431156542004 23.474199006983785 72-73 22.931621054441013 25.89481016194071 27.90096620087524 23.272602582743037 74-75 22.345497692375606 27.57039413984646 27.02727726468383 23.056830903094102 76-77 22.72567077226246 26.346084211453764 26.965075394612477 23.963169621671298 78-79 22.83093571637513 26.564547392888244 27.1784775327865 23.426039357950128 80-81 23.383219354980888 26.48115867868604 26.908046712222173 23.2275752541109 82-83 22.85156673300511 26.2282358887814 27.691681889999025 23.228515488214462 84-85 22.65812415000855 26.744833797853566 27.261073502828754 23.33596854930913 86-87 22.79296674598742 26.95072557090618 27.042694429706227 23.21361325340017 88-89 22.402780309626042 26.98483672593042 26.28220257309003 24.330180391353508 90-91 22.634693861103123 26.204877052813696 26.930999311363223 24.229429774719957 92-93 23.26499219231265 26.521782731401228 27.196610460868442 23.016614615417673 94-95 22.742210707066004 26.24239244678965 27.27463826632837 23.740758579815967 96-97 22.00169681151282 26.219695189908233 27.986323799482577 23.79228419909637 98-99 22.459032132335242 26.79551459883301 26.566297301479946 24.179155967351804 100-101 22.192358026383324 25.885862160702338 27.219270736760638 24.7025090761537 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 159.0 1 120.0 2 120.0 3 174.0 4 184.5 5 189.0 6 300.0 7 611.0 8 1003.0 9 1268.0 10 1232.5 11 1128.0 12 1193.0 13 1320.5 14 1437.5 15 1572.0 16 1704.5 17 1895.0 18 2104.0 19 2282.0 20 2612.5 21 3095.5 22 3741.5 23 4537.5 24 5542.0 25 7103.0 26 9492.0 27 12431.0 28 15693.5 29 19618.0 30 24774.0 31 31224.0 32 39050.5 33 43956.0 34 47519.5 35 52438.0 36 58384.5 37 64727.5 38 68521.0 39 70217.0 40 72421.5 41 77874.0 42 82711.5 43 87325.0 44 92567.5 45 97899.0 46 102560.0 47 121114.5 48 161408.0 49 159856.0 50 123558.0 51 107135.5 52 99507.0 53 97359.0 54 95965.0 55 89283.5 56 81481.5 57 73615.5 58 67193.5 59 60217.0 60 50515.5 61 42281.0 62 35514.0 63 27632.5 64 19985.0 65 15316.5 66 11799.5 67 9157.0 68 6805.0 69 4590.0 70 3319.5 71 2451.0 72 1875.5 73 1621.0 74 1290.0 75 968.0 76 704.0 77 435.5 78 277.5 79 184.0 80 124.0 81 78.0 82 55.5 83 40.5 84 24.5 85 19.5 86 14.5 87 11.0 88 7.0 89 6.0 90 5.5 91 4.5 92 2.5 93 1.5 94 4.0 95 4.0 96 2.0 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011565545131048726 2 0.011529738489776129 3 0.015217822540853588 4 0.017366221017209388 5 0.030149191951526405 6 0.046118953959104515 7 0.005442609473434695 8 0.025565941868634024 9 0.03358662951369568 10-11 0.03843842940613253 12-13 0.028233536643442476 14-15 0.026586431144903035 16-17 0.027105627443355682 18-19 0.02296996037637077 20-21 0.050934947210268776 22-23 0.012084741429501378 24-25 0.0026675947748084523 26-27 0.0011458125207230937 28-29 3.580664127259668E-5 30-31 0.0026138848128995575 32-33 0.0022200117589009937 34-35 0.0 36-37 3.222597714533701E-4 38-39 8.95166031814917E-5 40-41 0.001217425803268287 42-43 0.003580664127259668 44-45 0.001432265650903867 46-47 7.519394667245302E-4 48-49 7.161328254519336E-4 50-51 0.0030614678288070156 52-53 0.003634374089168563 54-55 0.004296796952711601 56-57 6.445195429067402E-4 58-59 0.0050487364194361315 60-61 0.0025780781716269606 62-63 4.654863365437568E-4 64-65 5.550029397252484E-4 66-67 0.015343145785307676 68-69 0.038760689177585905 70-71 0.049538488200637505 72-73 0.014251043226493478 74-75 0.029988062065799716 76-77 0.026317881335358558 78-79 0.05369205858825872 80-81 0.07578475625345087 82-83 0.04599363071465043 84-85 0.016847024718756737 86-87 0.018923809912567344 88-89 0.0204455921666527 90-91 0.036898743831410875 92-93 0.023560769957368614 94-95 0.011565545131048726 96-97 0.017258801093391597 98-99 0.04733637976237281 100-101 0.06482792402403628 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2792778.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.28477313279169 #Duplication Level Percentage of deduplicated Percentage of total 1 73.41529486865637 34.714255623414225 2 13.919108302821742 13.163237566193665 3 5.124457472288631 7.2692642701742125 4 2.439734038532213 4.614490820653814 5 1.3404404086242254 3.169121030991155 6 0.8378658793102617 2.377097881133565 7 0.5460293032148316 1.8073210208458745 8 0.3830664538875435 1.4490568293484405 9 0.292489801152527 1.2447282502037333 >10 1.509985851995895 13.029510854917751 >50 0.1160885387507152 3.8078991847173884 >100 0.06707256533681971 5.7409571938058255 >500 0.00494385002578008 1.5815000604362524 >1k 0.0026620730907886787 2.612492515796134 >5k 4.563553869923449E-4 1.2798663448966427 >10k+ 3.042369246615633E-4 2.1392005524713293 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22403 0.8021761844299834 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15101 0.5407160898574824 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11654 0.4172905973908417 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10323 0.36963195785701547 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7022 0.25143423501617385 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6861 0.24566936577128579 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5538 0.19829717936764038 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5500 0.1969365269992817 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5371 0.19231747027511675 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5295 0.18959616553839942 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4737 0.16961605970829047 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4380 0.15683308877397342 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4212 0.1508175730401772 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3551 0.1271493831589908 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3129 0.11203898054195499 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3124 0.11185994733559201 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3042 0.1089238027512391 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.580664127259668E-5 2 0.0 0.0 0.0 0.0 3.580664127259668E-5 3 0.0 0.0 0.0 0.0 3.580664127259668E-5 4 0.0 0.0 0.0 0.0 3.580664127259668E-5 5 0.0 0.0 0.0 0.0 3.580664127259668E-5 6 0.0 0.0 0.0 0.0 3.580664127259668E-5 7 2.1483984763558006E-4 0.0 0.0 0.0 3.580664127259668E-5 8 2.1483984763558006E-4 0.0 0.0 0.0 3.580664127259668E-5 9 2.1483984763558006E-4 0.0 0.0 0.0 3.580664127259668E-5 10-11 2.1483984763558006E-4 0.0 0.0 1.790332063629834E-5 7.161328254519336E-5 12-13 2.327431682718784E-4 0.0 0.0 3.580664127259668E-5 1.0741992381779003E-4 14-15 2.5064648890817673E-4 0.0 0.0 3.580664127259668E-5 1.0741992381779003E-4 16-17 2.5064648890817673E-4 0.0 0.0 3.580664127259668E-5 1.0741992381779003E-4 18-19 3.759697333622651E-4 0.0 0.0 7.161328254519336E-5 1.4322656509038672E-4 20-21 4.6548633654375677E-4 0.0 0.0 1.969365269992817E-4 1.4322656509038672E-4 22-23 5.012929778163535E-4 0.0 0.0 3.4016309208966845E-4 1.6112988572668507E-4 24-25 6.445195429067402E-4 0.0 0.0 5.370996190889501E-4 2.1483984763558006E-4 26-27 6.445195429067402E-4 0.0 0.0 7.519394667245302E-4 2.1483984763558006E-4 28-29 7.519394667245302E-4 0.0 0.0 0.001485975612812762 2.1483984763558006E-4 30-31 7.877461079971268E-4 0.0 0.0 0.003168887752624806 2.1483984763558006E-4 32-33 7.877461079971268E-4 0.0 0.0 0.005353092870253203 2.5064648890817673E-4 34-35 8.772627111786186E-4 0.0 0.0 0.00785955775933497 2.8645313018077343E-4 36-37 0.001002585955632707 0.0 0.0 0.011207478718322759 2.8645313018077343E-4 38-39 0.0010383925969053036 0.0 0.0 0.016739604794938946 2.8645313018077343E-4 40-41 0.0010383925969053036 0.0 0.0 0.02830514992598767 3.0435645081707175E-4 42-43 0.0010383925969053036 0.0 0.0 0.04080166773012391 3.580664127259668E-4 44-45 0.0010383925969053036 0.0 0.0 0.0571473994710643 4.1177637463486174E-4 46-47 0.0013427490477223754 0.0 0.0 0.08437835015887407 5.191962984526518E-4 48-49 0.001432265650903867 0.0 0.0 0.10357070988098588 5.729062603615469E-4 50-51 0.001432265650903867 0.0 0.0 0.13074795060688676 5.729062603615469E-4 52-53 0.001432265650903867 0.0 0.0 0.16336780080622232 6.266162222704418E-4 54-55 0.001432265650903867 0.0 0.0 0.2038293054442566 6.803261841793368E-4 56-57 0.0015038789334490604 0.0 0.0 0.28045551776761346 6.803261841793368E-4 58-59 0.0015038789334490604 0.0 0.0 0.3472886137029152 6.803261841793368E-4 60-61 0.0015217822540853588 0.0 0.0 0.40905506989814444 6.803261841793368E-4 62-63 0.001539685574721657 0.0 0.0 0.47511832304608526 7.519394667245302E-4 64-65 0.001539685574721657 0.0 0.0 0.5433836846322908 7.698427873608285E-4 66-67 0.001539685574721657 0.0 0.0 0.6518957110088951 7.877461079971268E-4 68-69 0.001539685574721657 0.0 0.0 0.7836104409301419 7.877461079971268E-4 70-71 0.0015933955366305521 0.0 0.0 0.8777102941945261 7.877461079971268E-4 72-73 0.0016112988572668503 0.0 0.0 0.9804216446849696 8.951660318149169E-4 74-75 0.0016112988572668503 0.0 0.0 1.1244359558833534 8.951660318149169E-4 76-77 0.0016112988572668503 0.0 0.0 1.2849750320290405 8.951660318149169E-4 78-79 0.0016292021779031488 0.0 0.0 1.4759855598977074 8.951660318149169E-4 80-81 0.0017903320636298338 0.0 0.0 1.6617504148199391 8.951660318149169E-4 82-83 0.001897751987447624 0.0 0.0 1.8707358766074496 9.667793143601103E-4 84-85 0.001969365269992817 0.0 0.0 2.1418995709648243 9.667793143601103E-4 86-87 0.002005171911265414 0.0 0.0 2.4000117445783378 0.0010204892762690054 88-89 0.002005171911265414 0.0 0.0 2.7237037816826115 0.0011458125207230937 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11665 0.0 28.224277 1 TACATGG 12000 0.0 26.80338 2 ACATGGG 11895 0.0 26.479858 3 CATGGGG 6085 0.0 22.173788 4 CATGGGA 5950 0.0 21.239624 4 GAGTACT 7155 0.0 20.119225 12-13 TACCTGG 2155 0.0 19.841629 2 GTACTTT 7430 0.0 19.375263 14-15 GTACCTG 2265 0.0 19.297186 1 AGTACTT 7555 0.0 18.299397 12-13 ATACCGA 185 1.5197405E-4 17.97696 6 ATGGGGA 3600 0.0 17.420528 5 ATGGGAG 2880 0.0 17.321548 5 AGAGTAC 14960 0.0 17.054556 10-11 AGTACAT 8805 0.0 16.780819 2 TAGTACG 200 2.7578144E-4 16.62839 4 CGTTCGG 130 6.655375E-6 16.436237 34-35 ACTTTTT 8925 0.0 16.183584 16-17 ACCGTCC 265 7.921295E-6 16.137098 8 GTGTAGC 1275 0.0 16.022587 1 >>END_MODULE