FastQCFastQC Report
Thu 26 May 2016
SRR936583_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936583_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2792778
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT291031.0420806809563812No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT110550.39584241926855623No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT102720.3678058191521131No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC82790.2964431830958279No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT78590.2814043937613373No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC72900.2610304148772298No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC72880.2609588015946846No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT61960.22185794932500902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55970.2004097712027236No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG55930.2002665446376332No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA55090.19725878677073508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51550.18458323576023586No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC46230.16553410260321444No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA45850.16417345023485577No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC33730.1207758010124686No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30400.10885218946869389No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT29570.10588023824306836No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA28910.103516999919077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG112700.023.7325531
GTAAGCG3150.023.37156794-95
TAAGGTG12850.022.5488555
TACATGG116800.022.0026762
ACATGGG114300.021.8593523
GTAAGGT12700.020.9450824
GAGTACT84100.020.10684812-13
TATACCG1200.00476490919.7918575
CATGGGG58850.019.7750424
TACCTGG20650.019.3232572
GTACTTT87400.019.10378814-15
AGGTAAG13200.018.7133062
GGTAAGG14550.018.6084273
AGTACTT88500.018.54363312-13
TAAGGTA7450.018.490114
GTGTAAG11650.018.3504451
TGCACCG6100.017.910015
GTACCTG23750.016.8025681
ACTTTTT103050.016.59374816-17
TACTTTT99400.016.1762514-15
Produced by FastQC (version 0.11.5)