##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936583_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2792778 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.800573837233035 31.0 31.0 34.0 30.0 34.0 2 31.92007921861315 31.0 31.0 34.0 30.0 34.0 3 32.0471036365941 33.0 31.0 34.0 30.0 34.0 4 35.788402801798064 37.0 35.0 37.0 35.0 37.0 5 35.643571741112254 37.0 35.0 37.0 33.0 37.0 6 35.6990838512764 37.0 35.0 37.0 33.0 37.0 7 35.62152165335018 37.0 35.0 37.0 33.0 37.0 8 35.58565306658818 37.0 35.0 37.0 33.0 37.0 9 37.17117150020517 39.0 37.0 39.0 33.0 39.0 10-11 37.05218961192046 39.0 37.0 39.0 33.0 39.0 12-13 36.93283461843369 39.0 37.0 39.0 33.0 39.0 14-15 37.971250489655816 40.0 37.0 41.0 33.0 41.0 16-17 37.9554876900348 40.0 37.0 41.0 33.0 41.0 18-19 38.09247315755137 40.0 37.0 41.0 33.0 41.0 20-21 38.08542927508023 40.0 37.0 41.0 33.0 41.0 22-23 37.98700541181576 40.0 37.0 41.0 33.0 41.0 24-25 37.84806042585554 40.0 37.0 41.0 32.5 41.0 26-27 37.62525449570285 39.0 37.0 41.0 32.0 41.0 28-29 37.68322956568693 39.5 37.0 41.0 32.0 41.0 30-31 37.60317343519607 39.0 37.0 41.0 32.0 41.0 32-33 37.44344555850841 39.0 36.0 41.0 32.0 41.0 34-35 37.25388376734563 39.0 36.0 41.0 31.0 41.0 36-37 37.076767469523176 39.0 36.0 41.0 31.0 41.0 38-39 36.88130689227715 39.0 35.5 40.0 31.0 41.0 40-41 36.70372134842082 39.0 35.0 40.0 30.0 41.0 42-43 36.450453097238665 39.0 35.0 40.0 30.0 41.0 44-45 36.195795190308715 38.0 35.0 40.0 30.0 41.0 46-47 35.8736073902043 38.0 35.0 40.0 29.0 41.0 48-49 35.51085711073347 38.0 34.5 40.0 28.0 41.0 50-51 35.20114685091332 38.0 34.0 40.0 27.0 41.0 52-53 34.94127370668202 38.0 34.0 40.0 26.5 41.0 54-55 34.606483401115305 38.0 33.0 40.0 26.0 41.0 56-57 34.22135432891551 37.0 33.0 40.0 25.5 41.0 58-59 33.82924528909924 37.0 32.5 39.0 24.0 40.5 60-61 33.377509597970196 36.0 32.0 39.0 23.0 40.0 62-63 33.046825060925 36.0 31.5 39.0 23.0 40.0 64-65 32.707304519012965 35.5 31.0 39.0 22.0 40.0 66-67 32.210441896921274 35.0 31.0 38.0 21.5 40.0 68-69 31.718274420666447 35.0 30.5 37.5 20.0 39.5 70-71 31.158064658200544 34.0 30.0 37.0 18.5 39.0 72-73 30.54816798900593 34.0 29.0 36.0 16.5 39.0 74-75 29.998241714880308 34.0 29.0 36.0 12.5 38.5 76-77 28.660470327394442 31.5 27.0 34.5 11.5 36.0 78-79 29.469425604183364 33.5 29.0 35.0 9.5 37.0 80-81 29.28671630899413 33.5 29.0 35.0 7.0 36.5 82-83 28.84960154369592 33.0 28.5 35.0 6.5 36.0 84-85 28.38170524116131 33.0 27.5 35.0 2.0 36.0 86-87 27.953773268050664 33.0 27.0 35.0 2.0 35.0 88-89 27.40212827514396 32.0 26.0 34.0 2.0 35.0 90-91 26.751834911332015 32.0 25.0 34.0 2.0 35.0 92-93 26.100542005128943 32.0 24.5 34.0 2.0 35.0 94-95 25.255933876591694 31.0 21.5 34.0 2.0 35.0 96-97 24.296909385565197 31.0 16.5 34.0 2.0 35.0 98-99 23.03084563112428 31.0 2.0 34.0 2.0 35.0 100-101 19.214685521011695 24.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2723398798271752 1101 2 0.3933322404291886 1101 3 0.24884271050566298 1101 4 0.2195338609988866 1101 5 0.24573639839746875 1101 6 0.2564708141676135 1101 7 0.24231526770233103 1101 8 0.1923846166943335 1101 9 0.32948844248665665 1101 10-11 0.28135313016723984 1101 12-13 0.2959096323745527 1101 14-15 0.3579642178239766 1101 16-17 0.3162063930801722 1101 18-19 0.33213053676252 1101 20-21 0.29026687152656194 1101 22-23 0.3489803466256163 1101 24-25 0.34469936876847385 1101 26-27 0.24182588858231213 1101 28-29 0.20553566383620137 1101 30-31 0.3540303041985524 1101 32-33 0.33924444694378053 1101 34-35 0.3916898287142061 1101 36-37 0.5389704071550483 1101 38-39 0.4781647852608444 1101 40-41 0.4470652132487061 1101 42-43 0.4582115791171262 1101 44-45 0.47340425724206625 1101 46-47 0.4551857033544735 1101 48-49 0.5097202437259085 1101 50-51 0.46713820802968087 1101 52-53 0.45862638092444286 1101 54-55 0.5126392709922456 1101 56-57 0.5171261381703545 1101 58-59 0.5054761628404165 1101 60-61 0.5525639703222751 1101 62-63 0.4876245327442348 1101 64-65 0.4927136439605988 1101 66-67 0.5057973693904074 1101 68-69 0.5464542079002932 1101 70-71 0.2452705234281254 1101 72-73 0.39455196266137094 1101 74-75 0.5905369415305977 1101 76-77 0.6432205705648215 1101 78-79 0.6215759573876234 1101 80-81 0.5917984109780186 1101 82-83 0.5528587018735713 1101 84-85 0.48889619517812477 1101 86-87 0.45869393272175785 1101 88-89 0.3462494505231746 1101 90-91 0.48354521375721404 1101 92-93 0.49987848698263093 1101 94-95 0.5402389977607953 1101 96-97 0.38066475961071333 1101 98-99 -0.07353499652308315 1101 100-101 0.09097800008220958 1102 1 0.22403539233897263 1102 2 0.3336396838204365 1102 3 0.2889124079203569 1102 4 0.2536478032714058 1102 5 0.1286629529440404 1102 6 0.14501705831278144 1102 7 0.2189054209029564 1102 8 0.08853115285072022 1102 9 0.16523186841828874 1102 10-11 0.2994659313432848 1102 12-13 0.31166168495341395 1102 14-15 0.35105987360443436 1102 16-17 0.41491544874388353 1102 18-19 0.3566980596199798 1102 20-21 0.29582497981177625 1102 22-23 0.2789729200244935 1102 24-25 0.22982890269266676 1102 26-27 0.3099457426609149 1102 28-29 0.21501826500982446 1102 30-31 0.4172927348077238 1102 32-33 0.29570593204294227 1102 34-35 0.3569819162523089 1102 36-37 0.35067422524910796 1102 38-39 0.2580123794052511 1102 40-41 0.2983746319790015 1102 42-43 0.3393751711919819 1102 44-45 0.31895279673857146 1102 46-47 0.2929367064193258 1102 48-49 0.3207635101250901 1102 50-51 0.3778160919694358 1102 52-53 0.3743864338106846 1102 54-55 0.5195919065408745 1102 56-57 0.5974219863792598 1102 58-59 0.5242733324158806 1102 60-61 0.44327806091627764 1102 62-63 0.36755952498341316 1102 64-65 0.3453644300171135 1102 66-67 0.43024394023233725 1102 68-69 0.4048081078132917 1102 70-71 0.1070641607626115 1102 72-73 0.05687609406220773 1102 74-75 0.36335031804453877 1102 76-77 0.34429579608664795 1102 78-79 0.3154394584589788 1102 80-81 0.3965943320844616 1102 82-83 0.31335673679806675 1102 84-85 0.15684343736164053 1102 86-87 0.2983493510615105 1102 88-89 0.10784621187242749 1102 90-91 0.24318268930232634 1102 92-93 0.2953591817672212 1102 94-95 0.3847188119852376 1102 96-97 0.40767277097114984 1102 98-99 0.3992539579999068 1102 100-101 0.30657115181909234 1103 1 0.1652444226382599 1103 2 0.2750338509172039 1103 3 0.23405755508343162 1103 4 0.13026106283948735 1103 5 0.15891365091148657 1103 6 0.11520460645458996 1103 7 0.11444467511611123 1103 8 0.1185371842268026 1103 9 0.04954351059374318 1103 10-11 0.15971599266069347 1103 12-13 0.12244821654000049 1103 14-15 0.21209295416444007 1103 16-17 0.09799097327158535 1103 18-19 0.1331406814706142 1103 20-21 0.15674649585212563 1103 22-23 0.09686684378999644 1103 24-25 0.1535073994197802 1103 26-27 0.20272043669199036 1103 28-29 0.1641103839629281 1103 30-31 0.3036168889225266 1103 32-33 0.18327336288780316 1103 34-35 0.11935250464014047 1103 36-37 0.3073100441107144 1103 38-39 0.04382155342031524 1103 40-41 0.16335477047924485 1103 42-43 0.3977814802129487 1103 44-45 0.43063564370660856 1103 46-47 0.40262844977475964 1103 48-49 0.2577562200083179 1103 50-51 0.3487075734626899 1103 52-53 0.37666166081002217 1103 54-55 0.31084861667290653 1103 56-57 0.3344941784446007 1103 58-59 0.27994475138648056 1103 60-61 0.07033716934949297 1103 62-63 0.3174973805307033 1103 64-65 0.32382558570665765 1103 66-67 0.1529608800253186 1103 68-69 0.1628798438855803 1103 70-71 -0.015917329089798926 1103 72-73 0.36211657676871667 1103 74-75 0.343142494451218 1103 76-77 0.0900340098981971 1103 78-79 0.010273366457276722 1103 80-81 0.48320578852868223 1103 82-83 0.34261619046963077 1103 84-85 0.361776691195562 1103 86-87 0.16145382722818624 1103 88-89 0.2668872497825667 1103 90-91 0.3517605104653505 1103 92-93 0.2856875286403522 1103 94-95 0.13893179408072598 1103 96-97 0.39712544405273675 1103 98-99 0.10441400225699127 1103 100-101 0.004003091646964663 1104 1 0.20204177299615012 1104 2 0.20514508487115535 1104 3 0.17568730899236584 1104 4 0.1254196331950439 1104 5 0.10126611355726567 1104 6 0.12171679527514101 1104 7 0.08254209444329064 1104 8 0.16520936476855042 1104 9 0.17456635317769553 1104 10-11 0.16895762064843467 1104 12-13 0.17411474667628113 1104 14-15 0.20644938261117574 1104 16-17 0.17218670353002352 1104 18-19 0.20355037824624134 1104 20-21 0.1570297483307712 1104 22-23 0.050616768461608785 1104 24-25 0.08484975878734247 1104 26-27 0.05531075243042238 1104 28-29 0.08514995029154449 1104 30-31 0.218187843734988 1104 32-33 0.23889517685211814 1104 34-35 0.1891591749497863 1104 36-37 0.20967336291916894 1104 38-39 0.20232157290141117 1104 40-41 0.19224133130069987 1104 42-43 0.19907265532955165 1104 44-45 0.26789846357711156 1104 46-47 0.14834671336029004 1104 48-49 0.22398833545214814 1104 50-51 0.21541693582909716 1104 52-53 0.2893011026371184 1104 54-55 0.13853854116523934 1104 56-57 0.2858721132229505 1104 58-59 0.3205955868592554 1104 60-61 0.07376033967738493 1104 62-63 0.1821114821996659 1104 64-65 0.24925793957424958 1104 66-67 0.23651752115769398 1104 68-69 0.09198010212770313 1104 70-71 -0.04797211969552606 1104 72-73 0.17146897631171143 1104 74-75 0.2997470875701076 1104 76-77 0.1528398220633278 1104 78-79 0.22951295935827787 1104 80-81 0.21937742737169685 1104 82-83 0.21055516946315223 1104 84-85 0.01771576746143566 1104 86-87 0.13489621150847597 1104 88-89 0.2012343574787714 1104 90-91 0.27395256780491906 1104 92-93 0.25597291028301683 1104 94-95 0.17378094604951855 1104 96-97 0.3450309954374333 1104 98-99 0.18323021368874848 1104 100-101 0.029618913953804338 1105 1 0.07818843870726155 1105 2 0.20044452331209328 1105 3 0.1497324794309094 1105 4 0.11670382200583873 1105 5 0.1530241210208274 1105 6 0.06044760526137338 1105 7 0.12756344085407534 1105 8 0.021749454556569958 1105 9 0.15189347028605482 1105 10-11 0.15615905244485617 1105 12-13 0.08556841421489025 1105 14-15 0.1765829640474763 1105 16-17 0.2489099654868241 1105 18-19 0.23223013857263197 1105 20-21 0.17425276969254355 1105 22-23 0.08223613943356867 1105 24-25 0.15301813948035914 1105 26-27 0.23704223045179873 1105 28-29 0.14780320165192506 1105 30-31 0.25036391067160224 1105 32-33 0.15000906788816337 1105 34-35 0.019858013950752706 1105 36-37 0.2192309367997609 1105 38-39 0.14743135078612113 1105 40-41 0.12318671622715982 1105 42-43 0.12645006703884576 1105 44-45 0.1749065619036969 1105 46-47 0.197884015829203 1105 48-49 0.21858518519286463 1105 50-51 0.49063302344664805 1105 52-53 0.32348063516607795 1105 54-55 0.39488263558087766 1105 56-57 0.5574690371898043 1105 58-59 0.5316633425170778 1105 60-61 0.4135182857124349 1105 62-63 0.3767272724007924 1105 64-65 0.23490190243046527 1105 66-67 0.17976780654436197 1105 68-69 0.27372981674103514 1105 70-71 0.23493405079933183 1105 72-73 0.1570943826357798 1105 74-75 0.35634771004577814 1105 76-77 0.20232561415850014 1105 78-79 0.10291741753313488 1105 80-81 0.05818067182137554 1105 82-83 0.055978616729330355 1105 84-85 0.12167777904071286 1105 86-87 0.14181427866008534 1105 88-89 0.25267754377611595 1105 90-91 0.503471915229273 1105 92-93 0.4265391148707032 1105 94-95 0.5448763957318832 1105 96-97 0.41391508237926544 1105 98-99 0.43060158492689027 1105 100-101 0.2529851712676354 1106 1 0.05999973209583587 1106 2 0.13717476200082857 1106 3 0.08345708525290263 1106 4 0.09190762963640964 1106 5 0.11527798511388454 1106 6 -0.02014924295007603 1106 7 0.04651106418872786 1106 8 0.054259615201438294 1106 9 0.14144187078830583 1106 10-11 0.04901949421267204 1106 12-13 0.11820955555248958 1106 14-15 0.15988387822672934 1106 16-17 0.17719383126743793 1106 18-19 0.055119662793515545 1106 20-21 -0.034607491053357364 1106 22-23 0.035348629127952336 1106 24-25 0.08685992632649686 1106 26-27 -0.03457023433812623 1106 28-29 0.16731769864517787 1106 30-31 0.08845518697646781 1106 32-33 0.11203855959200837 1106 34-35 0.12277310798178576 1106 36-37 0.26668130631492915 1106 38-39 0.3340688482677976 1106 40-41 0.07329101756062784 1106 42-43 0.34484520182397205 1106 44-45 0.2805637941195016 1106 46-47 0.30906636585794445 1106 48-49 0.3485986930704783 1106 50-51 0.41325252021316317 1106 52-53 0.3323370022681189 1106 54-55 0.25963542915418714 1106 56-57 0.28419382849421027 1106 58-59 0.2955227632023494 1106 60-61 0.34740188973609776 1106 62-63 0.34830608830187515 1106 64-65 0.27099807190266745 1106 66-67 0.07710013081371869 1106 68-69 0.20284273129794883 1106 70-71 0.2549792077754418 1106 72-73 0.35960628473377554 1106 74-75 0.2592609548282958 1106 76-77 0.10358577944972325 1106 78-79 0.06590182771764219 1106 80-81 0.09500441371906732 1106 82-83 0.0876800331607619 1106 84-85 0.24686995220323027 1106 86-87 0.15758553423849975 1106 88-89 0.04549452355741934 1106 90-91 0.07387466482949989 1106 92-93 -0.08883431995880642 1106 94-95 0.05363820954917031 1106 96-97 0.12557063558892878 1106 98-99 0.22968008301742415 1106 100-101 0.1512739937776928 1107 1 0.035865733525451304 1107 2 0.1518329764798665 1107 3 0.04025562699460039 1107 4 0.04446515577358667 1107 5 0.011123538259056431 1107 6 0.02333547370189848 1107 7 -0.04068804326455222 1107 8 0.0560221438256292 1107 9 0.058496144534395 1107 10-11 0.14139691018869627 1107 12-13 0.08633253781572847 1107 14-15 0.14093964312935725 1107 16-17 0.154427237429978 1107 18-19 0.05747554825590129 1107 20-21 0.06154895615212297 1107 22-23 0.0501619886865754 1107 24-25 0.1256222318907021 1107 26-27 0.12524995074127077 1107 28-29 0.09334249252557214 1107 30-31 0.23190044875371285 1107 32-33 0.11968068892443995 1107 34-35 0.10628722982660577 1107 36-37 0.2107191772432344 1107 38-39 0.2582696689790396 1107 40-41 0.26893744628576854 1107 42-43 0.31023792784427684 1107 44-45 0.20046229846725083 1107 46-47 0.3131696344872523 1107 48-49 0.20329269684016538 1107 50-51 0.17863922639349283 1107 52-53 0.10567195814643071 1107 54-55 0.14488419472753122 1107 56-57 0.2837077839307156 1107 58-59 0.408068464379987 1107 60-61 0.1410413440740328 1107 62-63 -0.07761637788468789 1107 64-65 0.12331826489030107 1107 66-67 0.16347822028329517 1107 68-69 0.2667097825569833 1107 70-71 0.06596011481770248 1107 72-73 0.2236158470770313 1107 74-75 0.08753145576237742 1107 76-77 0.060007841549740704 1107 78-79 0.25175369881906207 1107 80-81 0.31127071150118724 1107 82-83 0.401342357139157 1107 84-85 0.2508441968738744 1107 86-87 0.5002718744068133 1107 88-89 0.4634918416611953 1107 90-91 0.49630428182868513 1107 92-93 0.4813633886485391 1107 94-95 0.7183923149026974 1107 96-97 0.7118543535226145 1107 98-99 1.0180957924608371 1107 100-101 0.9270466972734468 1108 1 -0.07326718055428216 1108 2 0.13682156385569044 1108 3 -0.09172352165516529 1108 4 0.020423005101889657 1108 5 0.022917892807001294 1108 6 -0.006101229325295776 1108 7 0.028616408787755176 1108 8 0.016775129363942654 1108 9 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-1.6087394558517474 2316 54-55 -1.631020322498415 2316 56-57 -1.6192701471504591 2316 58-59 -1.9770167531683214 2316 60-61 -1.5671709117554116 2316 62-63 -1.4716533688081128 2316 64-65 -1.3252605147929053 2316 66-67 -1.4995104687845568 2316 68-69 -1.7817337323706646 2316 70-71 -1.328841219774489 2316 72-73 -1.2773544781827972 2316 74-75 -1.5687587691397304 2316 76-77 -1.657259554603712 2316 78-79 -1.7167028310155423 2316 80-81 -1.6128623866108995 2316 82-83 -1.6205339711524651 2316 84-85 -1.3750731471823379 2316 86-87 -1.656042499542309 2316 88-89 -1.700500349394627 2316 90-91 -1.94795738550879 2316 92-93 -1.9614649838486287 2316 94-95 -2.2921525432686707 2316 96-97 -1.923679727854406 2316 98-99 -1.4970552895443596 2316 100-101 -1.2441426620997333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 13.0 4 45.0 5 67.0 6 175.0 7 483.0 8 1074.0 9 2034.0 10 3178.0 11 4971.0 12 6983.0 13 9676.0 14 12530.0 15 16242.0 16 20350.0 17 24605.0 18 22913.0 19 19085.0 20 19367.0 21 21208.0 22 22953.0 23 25822.0 24 30954.0 25 37014.0 26 45047.0 27 55139.0 28 67844.0 29 82957.0 30 101362.0 31 123550.0 32 152695.0 33 192137.0 34 248946.0 35 328347.0 36 434373.0 37 467274.0 38 186616.0 39 4748.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.02643268353404 18.852320469608788 14.156411641095353 22.96483520576182 2 15.976899866716227 21.231438927599193 37.84410453859536 24.94755666708922 3 21.371423578696934 23.4819422974249 29.46214519569538 25.68448892818278 4 13.369541164565883 16.300542186491814 36.57914769801695 33.750768950925355 5 13.68065718718813 38.13435296990757 33.78801838697514 14.396971455929162 6 32.94099996490949 36.06978427930899 16.256322557682708 14.73289319809881 7 29.6447400647099 32.49624387526085 19.664405114345172 18.194610945684072 8 27.18565391476596 33.44930093592873 18.875231225028948 20.489813924276355 9 25.99423441255782 17.312087574482316 20.230437303085782 36.46324070987408 10-11 25.82391081568245 25.817698363421655 26.648663087434805 21.709727733461094 12-13 27.441529545133914 23.191585582527505 26.873904764360073 22.492980107978507 14-15 23.16386901707665 25.1658663236847 26.014864859542225 25.655399799696426 16-17 22.55364815703718 28.429347108951724 26.43270483536595 22.584299898645146 18-19 22.418051688380142 27.826833614061623 27.93439838208839 21.82071631546985 20-21 23.477538194254223 25.863938329174367 27.775468237011157 22.88305523956025 22-23 22.833924741385793 26.073414566899526 28.04183659950515 23.050824092209528 24-25 23.805943988962934 26.230167099271657 27.808635222866464 22.155253688898945 26-27 23.156517123186504 25.874510232197157 28.734351376004714 22.234621268611622 28-29 22.248898184297953 27.666571241916422 27.94323140430321 22.141299169482412 30-31 22.694865950101082 26.301863457232006 27.706635643659368 23.296634949007544 32-33 22.601112799848032 26.572720057450077 27.989009145498123 22.837157997203768 34-35 23.87053551686769 26.139159226719322 27.645810107275963 22.344495149137025 36-37 23.543673546117386 25.62444554849856 28.326883568541618 22.504997336842436 38-39 22.862452732897903 26.476506101753177 28.322769852182883 22.338271313166036 40-41 23.018942442931333 26.64101954471107 27.77188360255173 22.56815440980587 42-43 22.640127817308905 26.83740962267826 27.068724003987487 23.453738556025346 44-45 23.27267727100358 26.024311840697894 27.284127405303458 23.418883482995067 46-47 23.992293694665314 26.958981344023762 26.879383180474782 22.169341780836145 48-49 23.862953581421397 26.78308371987433 26.747795516768967 22.606167181935305 50-51 22.58992674376323 27.083269551691597 27.771735306455348 22.55506839808983 52-53 23.707929319269088 27.423148929435463 26.325236849412704 22.543684901882745 54-55 22.745525192071607 26.630784773630943 27.008151574053535 23.61553846024391 56-57 23.619128960319145 26.698914132393664 26.731036260941266 22.950920646345928 58-59 24.510328921678944 26.29776420198756 26.295078644753232 22.896828231580255 60-61 23.27424839522114 26.731454023732447 27.839227235397466 22.155070345648948 62-63 22.915852063827625 26.898364778793255 27.570743333147142 22.61503982423198 64-65 22.637495913428054 27.759416303246738 26.822557375828193 22.78053040749702 66-67 23.11775834525263 26.953327743841577 26.299579212604968 23.629334698300827 68-69 22.88346841353969 26.830780061112385 26.915804590035496 23.36994693531243 70-71 23.639595514558028 26.813623479907594 26.52165902841922 23.025121977115155 72-73 23.423398745117836 26.15748063920392 27.478213229705737 22.940907385972498 74-75 22.74771984229581 28.000963270392504 26.512549372083775 22.73876751522791 76-77 23.199209951375703 26.73704439672945 26.462564804200323 23.60118084769453 78-79 23.233120615857736 27.032206268511022 26.652469586509742 23.0822035291215 80-81 23.78045570903888 26.9061013648784 26.51243257875972 22.801010347323007 82-83 23.29688320226421 26.678427199164673 27.15184443296998 22.87284516560114 84-85 22.921276900490565 27.27027607691481 26.902818061374294 22.905628961220327 86-87 23.128699980771646 27.359564100057614 26.389803459026602 23.121932460144137 88-89 22.73800520784098 27.417148635448267 25.761482028938183 24.083364127772576 90-91 23.143582748770562 26.539448094442953 26.380256275373377 23.936712881413104 92-93 23.599140111143875 27.080304216029976 26.577311846496197 22.74324382632995 94-95 23.13796008746912 26.79180465754808 26.650868657745736 23.41936659723707 96-97 22.362272193264545 26.72592795762491 27.402155492050372 23.509644357060168 98-99 22.764527489675494 27.3965593393032 25.896782327517137 23.942130843504167 100-101 22.36959398405241 26.698345567863193 26.469499792405983 24.462560655678416 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 248.0 1 188.0 2 149.0 3 198.5 4 188.5 5 176.0 6 302.5 7 612.5 8 976.0 9 1192.0 10 1174.0 11 1087.5 12 1164.0 13 1308.5 14 1500.0 15 1694.5 16 1849.0 17 2035.5 18 2234.5 19 2477.5 20 2839.0 21 3269.0 22 3869.0 23 4765.5 24 5835.5 25 7403.5 26 9737.5 27 12750.5 28 16054.5 29 19908.5 30 25088.0 31 31490.0 32 39189.5 33 43947.0 34 47614.0 35 52692.5 36 58291.5 37 64634.5 38 68659.0 39 70197.5 40 72507.5 41 78038.0 42 82769.0 43 86876.5 44 91844.0 45 96964.5 46 101188.0 47 120766.5 48 163379.5 49 161099.5 50 122820.5 51 106625.5 52 99550.5 53 97446.0 54 95842.0 55 88991.5 56 80436.5 57 72652.5 58 67341.0 59 60805.0 60 50863.5 61 42245.0 62 35215.5 63 27285.0 64 19849.0 65 15238.5 66 11627.0 67 9144.0 68 6785.5 69 4517.0 70 3242.5 71 2368.5 72 1847.0 73 1590.0 74 1259.5 75 941.0 76 709.5 77 446.5 78 261.0 79 185.0 80 124.5 81 80.0 82 54.5 83 32.5 84 21.0 85 16.0 86 7.0 87 2.0 88 1.5 89 1.5 90 2.0 91 2.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011672965054866516 2 0.008593593905423202 3 0.005263576267071712 4 4.2967969527116007E-4 5 0.005335189549616905 6 0.0 7 2.1483984763558003E-4 8 0.001575492215994254 9 3.580664127259668E-5 10-11 0.0 12-13 0.0 14-15 6.087129016341435E-4 16-17 0.00409986042571232 18-19 0.0015038789334490604 20-21 5.370996190889501E-4 22-23 2.8645313018077343E-4 24-25 2.5064648890817673E-4 26-27 1.790332063629834E-5 28-29 5.370996190889501E-5 30-31 4.2967969527116007E-4 32-33 0.003312114317715193 34-35 9.846826349964087E-4 36-37 0.0014501689715401653 38-39 0.004941316495618341 40-41 7.161328254519336E-5 42-43 0.0015038789334490604 44-45 0.005263576267071712 46-47 0.0 48-49 0.0021483984763558004 50-51 0.0016650088191757454 52-53 8.056494286334252E-4 54-55 1.6112988572668504E-4 56-57 0.011117962115141268 58-59 0.0022021084382646957 60-61 0.0037059873717137557 62-63 2.1483984763558003E-4 64-65 0.003401630920896684 66-67 0.0013427490477223754 68-69 0.001951461949356519 70-71 0.005138253022617623 72-73 0.0030614678288070156 74-75 0.007447781384700109 76-77 0.001378555688994972 78-79 0.006624228635430386 80-81 0.0026854980954447508 82-83 0.0037238906923500546 84-85 0.002792918019262541 86-87 9.667793143601102E-4 88-89 0.0023632383239913807 90-91 0.00841456069906022 92-93 0.0032763076764425955 94-95 7.519394667245302E-4 96-97 1.790332063629834E-4 98-99 0.0010562959175416018 100-101 0.0024169482859002756 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2792778.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.624840961523645 #Duplication Level Percentage of deduplicated Percentage of total 1 71.95124518326828 32.827641184702095 2 14.672250798941496 13.38838218498588 3 5.369188624908504 7.34905131311617 4 2.6002354796682 4.745413208894911 5 1.4223081181813928 3.244629084515501 6 0.8780745103603457 2.4037205932535115 7 0.5695210053123712 1.8189013704136794 8 0.41727958326653514 1.5230651698421223 9 0.3025149200444245 1.242197560396342 >10 1.6093777618457645 13.37492028710232 >50 0.12882706237989594 4.0954151632003475 >100 0.07021040933242222 5.88370582175402 >500 0.005112502297111788 1.6014356204557754 >1k 0.00291019361526726 2.582945629301208 >5k 7.078849334433876E-4 2.1088595364007223 >10k+ 2.3596164448112916E-4 1.809716271665392 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 29103 1.0420806809563812 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11055 0.39584241926855623 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10272 0.3678058191521131 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8279 0.2964431830958279 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7859 0.2814043937613373 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7290 0.2610304148772298 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7288 0.2609588015946846 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6196 0.22185794932500902 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5597 0.2004097712027236 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5593 0.2002665446376332 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5509 0.19725878677073508 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5155 0.18458323576023586 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4623 0.16553410260321444 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4585 0.16417345023485577 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3373 0.1207758010124686 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3040 0.10885218946869389 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2957 0.10588023824306836 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2891 0.103516999919077 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.580664127259668E-5 0.0 0.0 0.0 0.0 7 1.0741992381779003E-4 0.0 0.0 0.0 0.0 8 1.0741992381779003E-4 0.0 0.0 0.0 0.0 9 1.0741992381779003E-4 0.0 0.0 0.0 0.0 10-11 1.0741992381779003E-4 0.0 0.0 1.790332063629834E-5 0.0 12-13 1.0741992381779003E-4 0.0 0.0 3.580664127259668E-5 1.790332063629834E-5 14-15 2.1483984763558006E-4 0.0 0.0 3.580664127259668E-5 3.580664127259668E-5 16-17 2.1483984763558006E-4 0.0 0.0 3.580664127259668E-5 3.580664127259668E-5 18-19 2.864531301807734E-4 0.0 0.0 7.161328254519336E-5 3.580664127259668E-5 20-21 3.222597714533701E-4 0.0 0.0 1.969365269992817E-4 5.370996190889502E-5 22-23 3.222597714533701E-4 0.0 0.0 3.2225977145337013E-4 1.0741992381779004E-4 24-25 4.296796952711601E-4 0.0 0.0 5.370996190889501E-4 1.790332063629834E-4 26-27 4.296796952711601E-4 0.0 0.0 8.235527492697235E-4 1.790332063629834E-4 28-29 7.161328254519336E-4 0.0 0.0 0.0015575888953579553 1.790332063629834E-4 30-31 8.235527492697236E-4 0.0 0.0 0.003240501035169999 1.790332063629834E-4 32-33 8.414560699060219E-4 0.0 0.0 0.005460512794070994 1.790332063629834E-4 34-35 8.593593905423202E-4 0.0 0.0 0.00809230092760685 2.1483984763558006E-4 36-37 8.951660318149169E-4 0.0 0.0 0.01142231856595834 2.1483984763558006E-4 38-39 0.001002585955632707 0.0 0.0 0.016775411436211543 2.1483984763558006E-4 40-41 0.0010204892762690054 0.0 0.0 0.028287246605351375 2.5064648890817673E-4 42-43 0.001110005879450497 0.0 0.0 0.04080166773012391 2.5064648890817673E-4 44-45 0.001217425803268287 0.0 0.0 0.057219012753609494 2.6854980954447505E-4 46-47 0.0013069424064497785 0.0 0.0 0.08482593317478153 3.222597714533701E-4 48-49 0.0013964590096312705 0.0 0.0 0.10426893938580152 3.222597714533701E-4 50-51 0.0013964590096312705 0.0 0.0 0.1313924701497935 3.580664127259668E-4 52-53 0.0013964590096312705 0.0 0.0 0.16419135355549205 4.2967969527116007E-4 54-55 0.0013964590096312705 0.0 0.0 0.20425898513952773 4.6548633654375677E-4 56-57 0.001432265650903867 0.0 0.0 0.28084939082161203 4.6548633654375677E-4 58-59 0.001432265650903867 0.0 0.0 0.34775410003945895 4.6548633654375677E-4 60-61 0.001432265650903867 0.0 0.0 0.4093057163870526 4.6548633654375677E-4 62-63 0.001432265650903867 0.0 0.0 0.4755121961000839 5.370996190889501E-4 64-65 0.001432265650903867 0.0 0.0 0.5435090078767449 5.370996190889501E-4 66-67 0.0015754922159942537 0.0 0.0 0.6518061944057136 5.370996190889501E-4 68-69 0.0016829121398120438 0.0 0.0 0.7831628579142345 5.370996190889501E-4 70-71 0.0016829121398120438 0.0 0.0 0.8769404514071653 5.729062603615469E-4 72-73 0.0017008154604483423 0.0 0.0 0.9796518018976088 6.266162222704418E-4 74-75 0.0017187187810846405 0.0 0.0 1.123737726378538 6.445195429067402E-4 76-77 0.0017187187810846405 0.0 0.0 1.2848676121052227 6.445195429067402E-4 78-79 0.0017187187810846405 1.790332063629834E-5 0.0 1.476075076500889 6.624228635430385E-4 80-81 0.0018261387049024304 3.580664127259668E-5 0.0 1.6614997683310309 6.982295048156351E-4 82-83 0.001861945346175027 3.580664127259668E-5 0.0 1.8694826441629089 7.519394667245302E-4 84-85 0.002005171911265414 3.580664127259668E-5 0.0 2.138444230082019 7.519394667245302E-4 86-87 0.0021483984763558004 3.580664127259668E-5 0.0 2.392170090139639 7.877461079971268E-4 88-89 0.0021483984763558004 3.580664127259668E-5 0.0 2.710723874221295 8.235527492697236E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 11270 0.0 23.732553 1 GTAAGCG 315 0.0 23.371567 94-95 TAAGGTG 1285 0.0 22.548855 5 TACATGG 11680 0.0 22.002676 2 ACATGGG 11430 0.0 21.859352 3 GTAAGGT 1270 0.0 20.945082 4 GAGTACT 8410 0.0 20.106848 12-13 TATACCG 120 0.004764909 19.791857 5 CATGGGG 5885 0.0 19.775042 4 TACCTGG 2065 0.0 19.323257 2 GTACTTT 8740 0.0 19.103788 14-15 AGGTAAG 1320 0.0 18.713306 2 GGTAAGG 1455 0.0 18.608427 3 AGTACTT 8850 0.0 18.543633 12-13 TAAGGTA 745 0.0 18.49011 4 GTGTAAG 1165 0.0 18.350445 1 TGCACCG 610 0.0 17.91001 5 GTACCTG 2375 0.0 16.802568 1 ACTTTTT 10305 0.0 16.593748 16-17 TACTTTT 9940 0.0 16.17625 14-15 >>END_MODULE