FastQCFastQC Report
Thu 26 May 2016
SRR936582_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936582_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998462
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT239240.7978757109478126No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT150090.5005566186931834No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT117460.39173416238058045No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC105100.35051302967988257No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT73340.24459206086320254No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT72900.24312464189974728No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC60530.20187015876806177No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59540.19856846610028742No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC58080.19369930317609496No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC55770.18599535361795483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47560.15861464977711906No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG47090.15704717952070096No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA44850.14957668297947416No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA36860.12292968862036603No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT33570.11195739682543918No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA32990.11002307182815725No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC31000.10338633606162093No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG119500.027.2807351
TACATGG124150.025.4933762
ACATGGG121250.024.92733
GAGTACT74250.020.89311812-13
CATGGGG59650.020.8736294
GTACTTT77700.019.50534214-15
TAAGGTG13250.019.3683345
GTACCTG23450.019.2522131
CATGGGA65350.018.8348124
GTGTAGC12950.018.715451
ATACCGT2803.4748155E-818.669586
CTGGACG9350.018.2977644
TACCTGG24550.018.1960122
GTAAGGT10650.017.8491484
AGTACTT79650.017.74670412-13
GTATAGC8200.017.3863031
ACCGTGC5500.017.2800668
ATTCGCG4300.017.12336780-81
ATGGGGA35550.016.710325
ATGGGAG31350.016.675145
Produced by FastQC (version 0.11.5)