FastQCFastQC Report
Thu 26 May 2016
SRR936582_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936582_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2998462
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT289780.9664287891592424No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT118730.3959696671160081No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT105800.35284755984901595No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC87990.29345044226006534No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT79610.26550278109244No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC73840.2462595824125835No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC73130.2438917018124625No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT62700.2091072022923752No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58980.19670084196498072No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG56110.18712926827153387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54120.18049253250499755No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA53650.17892506224857943No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC49380.16468442821686585No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA46590.15537965797131997No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC34580.11532579035518876No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33130.1104899778619839No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT30960.10325293433767044No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA30670.10228577183902948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG120000.024.5617641
TACATGG128250.022.2947252
ACATGGG126500.022.0005683
TAAGGTG13650.021.918615
GTAAGGT12200.019.8535024
CATGGGG61350.019.6628274
GAGTACT86600.019.49418412-13
GTACTTT88700.018.71142814-15
AGTACTT91850.017.4751512-13
CATGGGA67400.016.5590274
GTACCTG24100.016.1339381
ATTCGCG4450.016.01117780-81
AGTACAT97250.015.9217582
ACTTTTT106850.015.88896916-17
AGGTAAG14350.015.8873832
TACCTGG22650.015.7273692
TACTTTT100450.015.69536914-15
GTATAGG9800.015.6745951
GGTAAGG14850.015.3511733
AGAGTAC179050.015.17068210-11
Produced by FastQC (version 0.11.5)