##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936582_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2998462 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.330657517087094 31.0 31.0 34.0 30.0 34.0 2 31.547528366209075 31.0 31.0 34.0 30.0 34.0 3 31.835826500385863 31.0 31.0 34.0 30.0 34.0 4 35.65414002245151 37.0 35.0 37.0 33.0 37.0 5 35.4483105005166 37.0 35.0 37.0 33.0 37.0 6 35.55712662024732 37.0 35.0 37.0 33.0 37.0 7 35.488000181426344 37.0 35.0 37.0 33.0 37.0 8 35.459519246867224 37.0 35.0 37.0 33.0 37.0 9 37.061791011525244 39.0 37.0 39.0 33.0 39.0 10-11 36.927837838198386 39.0 37.0 39.0 33.0 39.0 12-13 36.80578159736558 39.0 37.0 39.0 32.0 39.0 14-15 37.87043591014327 40.0 37.0 41.0 33.0 41.0 16-17 37.78726043551661 39.0 37.0 41.0 32.5 41.0 18-19 37.83022546225365 39.5 37.0 41.0 32.5 41.0 20-21 37.89924434593468 40.0 37.0 41.0 33.0 41.0 22-23 37.810317589484214 39.5 37.0 41.0 32.0 41.0 24-25 37.685000510261595 39.0 37.0 41.0 32.0 41.0 26-27 37.458263102884075 39.0 36.0 41.0 32.0 41.0 28-29 37.398099425638875 39.0 36.0 41.0 31.5 41.0 30-31 37.44293908010174 39.0 36.0 41.0 32.0 41.0 32-33 37.30836175345894 39.0 36.0 41.0 31.0 41.0 34-35 37.139657264290825 39.0 36.0 41.0 31.0 41.0 36-37 36.918110517992226 39.0 35.5 41.0 30.5 41.0 38-39 36.76963206470517 39.0 35.0 40.0 30.0 41.0 40-41 36.58481848360926 39.0 35.0 40.0 30.0 41.0 42-43 36.355677010414006 39.0 35.0 40.0 30.0 41.0 44-45 36.11905019973573 38.0 35.0 40.0 30.0 41.0 46-47 35.786680471521734 38.0 35.0 40.0 28.5 41.0 48-49 35.37747768689415 38.0 34.0 40.0 27.0 41.0 50-51 35.18649094102243 38.0 34.0 40.0 27.0 41.0 52-53 34.918894253120435 38.0 34.0 40.0 26.5 41.0 54-55 34.64022172033529 38.0 33.0 40.0 26.0 41.0 56-57 34.32499227937522 37.5 33.0 40.0 25.5 41.0 58-59 33.93696501739892 37.0 33.0 40.0 24.5 41.0 60-61 33.49791876635422 36.0 32.0 39.0 23.0 40.5 62-63 33.21047657098873 36.0 32.0 39.0 23.0 40.0 64-65 32.75909432902601 35.5 31.0 39.0 22.0 40.0 66-67 32.3076632286819 35.0 31.0 38.0 22.0 40.0 68-69 31.82076561250401 35.0 30.5 37.5 20.5 39.5 70-71 31.29616033152996 34.0 30.0 37.0 19.5 39.0 72-73 30.846517314543256 34.0 29.5 36.0 18.0 39.0 74-75 30.465554340858745 34.0 29.0 36.0 17.5 38.5 76-77 29.06216103455705 32.5 28.0 34.5 14.0 36.5 78-79 29.898799951441774 34.0 29.0 35.0 15.5 37.0 80-81 29.727133110241184 34.0 29.0 35.0 11.5 37.0 82-83 29.371317195282113 34.0 29.0 35.0 7.5 36.0 84-85 28.896651016421085 33.0 29.0 35.0 6.5 36.0 86-87 28.519168326962287 33.0 28.0 35.0 2.0 35.5 88-89 28.069403747654633 33.0 27.0 35.0 2.0 35.0 90-91 27.552437216146146 32.5 26.0 34.5 2.0 35.0 92-93 27.04882453070941 32.0 25.5 34.0 2.0 35.0 94-95 26.439520160669034 32.0 25.0 34.0 2.0 35.0 96-97 25.649483135020553 32.0 23.5 34.0 2.0 35.0 98-99 24.686545135472784 31.0 19.0 34.0 2.0 35.0 100-101 21.79010406001477 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.25438513064477775 1101 2 0.27764737605167866 1101 3 0.14347702131287932 1101 4 0.08419099479154113 1101 5 0.13018710696103142 1101 6 0.1202979517671281 1101 7 0.12342789173043656 1101 8 -0.022810394263295564 1101 9 0.20183683300645328 1101 10-11 0.21143632787023137 1101 12-13 0.07455740478721395 1101 14-15 0.1239408274919711 1101 16-17 0.12613083232669453 1101 18-19 0.1686937415486156 1101 20-21 -0.10340865077088779 1101 22-23 -0.01792067301487066 1101 24-25 -0.11392775681309075 1101 26-27 0.05793859896551368 1101 28-29 0.015139603598747442 1101 30-31 -0.04087519091090286 1101 32-33 -0.030270677333817275 1101 34-35 0.15486477516170538 1101 36-37 0.07627293762435983 1101 38-39 0.06370806090614423 1101 40-41 0.08764935066768231 1101 42-43 0.07812420265999975 1101 44-45 0.07743967156735465 1101 46-47 0.15728948739901938 1101 48-49 0.11700694316540705 1101 50-51 0.055793725161699115 1101 52-53 0.09465462643643718 1101 54-55 0.08134861334271903 1101 56-57 0.04971695988029268 1101 58-59 0.1100888305632921 1101 60-61 0.1503700660353431 1101 62-63 0.06504680800208718 1101 64-65 0.2510354729070343 1101 66-67 -0.04896973320489195 1101 68-69 -0.151259815330679 1101 70-71 -0.10438916656898556 1101 72-73 -0.05116328815531901 1101 74-75 -0.10853845737029033 1101 76-77 0.2107465608046688 1101 78-79 0.187626890000125 1101 80-81 0.11795587319316425 1101 82-83 0.15095777230686735 1101 84-85 0.10965792820449849 1101 86-87 0.0959186436112418 1101 88-89 -0.12538214724539998 1101 90-91 -0.21907207355032554 1101 92-93 -0.07735043620978033 1101 94-95 -0.35296659759104543 1101 96-97 -0.26740248387168464 1101 98-99 -0.20848781894098423 1101 100-101 -0.09102300386731699 1102 1 0.3360762383511684 1102 2 0.46790080675121715 1102 3 0.17299048875688428 1102 4 0.09536389939520973 1102 5 0.08367550990323736 1102 6 0.1279859355305888 1102 7 0.12192213133884877 1102 8 0.007695140620732843 1102 9 0.04860129699488169 1102 10-11 0.14633022324798617 1102 12-13 0.1602300474041627 1102 14-15 0.26115894304469833 1102 16-17 0.26957809672104105 1102 18-19 0.07605059399372038 1102 20-21 0.1787381148538003 1102 22-23 0.09422755177637043 1102 24-25 0.09308320683376081 1102 26-27 -0.14763931389571638 1102 28-29 0.023239138655227976 1102 30-31 0.23925178974187844 1102 32-33 0.26054320597265246 1102 34-35 0.2993579966698192 1102 36-37 0.36782461201797645 1102 38-39 0.20988802920243899 1102 40-41 0.3317163463100812 1102 42-43 0.13194970623826663 1102 44-45 0.12625965352674484 1102 46-47 0.1927439182909012 1102 48-49 0.26442794919441326 1102 50-51 0.21547032079348583 1102 52-53 0.0334491393069456 1102 54-55 0.18408944214932177 1102 56-57 0.24667368564320924 1102 58-59 0.19896293433656353 1102 60-61 0.10766208505756936 1102 62-63 0.1911898494357942 1102 64-65 0.08747948402322692 1102 66-67 0.16441347709416476 1102 68-69 0.11159502744412819 1102 70-71 -0.19485019687322236 1102 72-73 -0.09390882609033113 1102 74-75 -0.49608654354070936 1102 76-77 -0.2458167869213561 1102 78-79 0.08100467816893797 1102 80-81 0.0314294523297356 1102 82-83 0.10941588096171273 1102 84-85 0.3464490717267914 1102 86-87 0.16658864977953414 1102 88-89 0.0697155505877376 1102 90-91 0.025140507118369015 1102 92-93 0.11949900764878763 1102 94-95 -0.0643917058678376 1102 96-97 -0.06044748257833277 1102 98-99 -0.5101876650652386 1102 100-101 -0.5742958149516753 1103 1 0.37923043962584657 1103 2 0.42419401593490846 1103 3 0.2185924648704436 1103 4 0.14464294654019483 1103 5 0.07849805334422655 1103 6 0.052013820399359645 1103 7 0.04207082786519578 1103 8 0.07513692920846893 1103 9 -0.054206140922161694 1103 10-11 0.12906724951266568 1103 12-13 0.20171427981386358 1103 14-15 0.1992264168792488 1103 16-17 0.15829014990757884 1103 18-19 0.07624547249559299 1103 20-21 0.04055721945378821 1103 22-23 0.05020192801875112 1103 24-25 0.03328983010010944 1103 26-27 0.05777592534285958 1103 28-29 0.16786265551270674 1103 30-31 0.16626173863100036 1103 32-33 0.06791914579781633 1103 34-35 0.07673488811371243 1103 36-37 0.12308035345096613 1103 38-39 0.13445573402820088 1103 40-41 0.2428222248737768 1103 42-43 0.21236775153207077 1103 44-45 0.28771962372562854 1103 46-47 0.18406873422994607 1103 48-49 0.29815978303690827 1103 50-51 0.17509126746705306 1103 52-53 0.25143818999813305 1103 54-55 0.27204150171252905 1103 56-57 0.26767074619869646 1103 58-59 0.26362863962431504 1103 60-61 0.2838908932405104 1103 62-63 0.10839344866928968 1103 64-65 0.1361348548942658 1103 66-67 0.12000704317554778 1103 68-69 0.18303186598777543 1103 70-71 0.156853932664049 1103 72-73 0.19169891667004535 1103 74-75 0.30730174780753217 1103 76-77 0.11762840634671434 1103 78-79 0.3112867721205461 1103 80-81 0.22532111745103833 1103 82-83 0.3046736069670928 1103 84-85 0.4170600353505556 1103 86-87 0.3701578300210002 1103 88-89 0.3542078253957044 1103 90-91 0.14681699904506829 1103 92-93 0.1817659830628635 1103 94-95 0.14940131624550546 1103 96-97 -0.23130147498490317 1103 98-99 -0.1366819098053078 1103 100-101 0.1621432928366353 1104 1 0.3082238225677152 1104 2 0.3722447386813563 1104 3 0.16424167052530692 1104 4 0.08064777261012068 1104 5 0.0772466896813171 1104 6 0.12222014959238692 1104 7 0.12042315139159143 1104 8 0.07110510230656075 1104 9 -0.05090982406796485 1104 10-11 -0.04206667678684539 1104 12-13 -0.08854588930280727 1104 14-15 0.11051021437872777 1104 16-17 0.05394578841632125 1104 18-19 0.0460262122452022 1104 20-21 -0.059177992027819926 1104 22-23 -0.014957898788395596 1104 24-25 0.04452620827675702 1104 26-27 0.07481988399451467 1104 28-29 -0.07032084542199613 1104 30-31 0.05504643570893819 1104 32-33 0.1112149480237008 1104 34-35 0.16662860919905853 1104 36-37 0.16702536337390228 1104 38-39 0.1460476550172345 1104 40-41 0.2289634478675282 1104 42-43 0.1299008929825618 1104 44-45 0.06521758257088095 1104 46-47 0.2675063206395194 1104 48-49 0.35220535378216766 1104 50-51 0.3598923738362245 1104 52-53 0.2131146366448604 1104 54-55 0.3948192774481285 1104 56-57 0.3148909568777256 1104 58-59 0.20244541928456528 1104 60-61 -0.04036125175887406 1104 62-63 -0.2966410457226871 1104 64-65 -0.2007749818174389 1104 66-67 -0.05049670850566912 1104 68-69 0.04271596589265059 1104 70-71 -0.0570112159307925 1104 72-73 -0.043437984671214735 1104 74-75 -0.117677452631618 1104 76-77 0.1509308619814682 1104 78-79 0.10841735170230038 1104 80-81 0.20519792523613845 1104 82-83 0.2500830203357509 1104 84-85 0.3079153353482269 1104 86-87 0.2810777526307078 1104 88-89 0.2162909100941306 1104 90-91 0.274086867271631 1104 92-93 0.3552400386484429 1104 94-95 0.27648628587222746 1104 96-97 0.1700701319910074 1104 98-99 0.4357899684123936 1104 100-101 0.39429526829458084 1105 1 0.3517529150959646 1105 2 0.436889331941682 1105 3 0.1787433671809353 1105 4 0.10574680351687249 1105 5 -0.09887344679968635 1105 6 -0.008118640458839366 1105 7 0.04288812494198879 1105 8 -0.04502433414991458 1105 9 0.031027841687013336 1105 10-11 0.10653663857711848 1105 12-13 0.07692166635364117 1105 14-15 0.06135357895200144 1105 16-17 0.16103789438071203 1105 18-19 0.0929909256322432 1105 20-21 0.07265623896062579 1105 22-23 -0.005811010842521114 1105 24-25 -0.05640932206389948 1105 26-27 0.002261727619050191 1105 28-29 -0.02172058123109366 1105 30-31 0.005405233318931835 1105 32-33 0.09418293389555288 1105 34-35 0.04584405903757016 1105 36-37 0.2032733399376383 1105 38-39 0.10096710201450065 1105 40-41 0.11022586868450901 1105 42-43 0.06518233928741068 1105 44-45 0.013530060119329335 1105 46-47 0.25497555972984287 1105 48-49 0.301962704159763 1105 50-51 0.07275249870649958 1105 52-53 0.14608435151477295 1105 54-55 0.27538753605416133 1105 56-57 0.1586654044018161 1105 58-59 0.11804993192883728 1105 60-61 0.0855562258078848 1105 62-63 0.028007469312065325 1105 64-65 0.137948716562299 1105 66-67 0.1670067322004769 1105 68-69 -0.022478489434309523 1105 70-71 -0.055145443949427886 1105 72-73 -0.12537312812313317 1105 74-75 -0.10839859128058293 1105 76-77 -0.09072704781853602 1105 78-79 -0.050505315706409704 1105 80-81 -0.24327630516647858 1105 82-83 -0.008610239541610554 1105 84-85 0.11320594737692957 1105 86-87 -0.04495571019440803 1105 88-89 0.18018068382320607 1105 90-91 -0.02833458434632874 1105 92-93 0.14833165398420434 1105 94-95 0.34596389656776694 1105 96-97 0.12581665029743405 1105 98-99 0.4076031910573086 1105 100-101 0.564924879888391 1106 1 0.31033617914642875 1106 2 0.348133892064709 1106 3 0.08665891556436378 1106 4 0.009657709566688766 1106 5 -0.11891203043332155 1106 6 -0.11204326680559973 1106 7 -0.049253562716671695 1106 8 0.011850139687325623 1106 9 -0.07072958280883768 1106 10-11 -0.1206351339805849 1106 12-13 -0.09111906052446983 1106 14-15 0.04297582172832648 1106 16-17 -0.025893874369977254 1106 18-19 -0.14055040075252379 1106 20-21 -0.12061683354207275 1106 22-23 -0.03001805453880735 1106 24-25 -0.18896435697134706 1106 26-27 -0.11726855090134336 1106 28-29 -0.09294282256833952 1106 30-31 -0.11696804117792681 1106 32-33 -0.18341497734867573 1106 34-35 -0.3494245726023877 1106 36-37 -0.1057952775731934 1106 38-39 -0.08071755257743263 1106 40-41 -0.1490791173270054 1106 42-43 -0.3392736582975573 1106 44-45 -0.19802195566865777 1106 46-47 -0.15137527791146255 1106 48-49 -0.2732700666468517 1106 50-51 -0.16980583463227816 1106 52-53 -0.07838747428881021 1106 54-55 -0.285047755493153 1106 56-57 -0.27644249698473544 1106 58-59 -0.4130316919002013 1106 60-61 -0.14296296494643457 1106 62-63 -0.5495040727446536 1106 64-65 -0.34957881397394175 1106 66-67 -0.42345855266821175 1106 68-69 -0.32838583769175145 1106 70-71 -0.33380831352255314 1106 72-73 -0.11487350326450851 1106 74-75 -0.11738391399701342 1106 76-77 -0.3333964599618646 1106 78-79 -0.18731478738830276 1106 80-81 -0.33222197311763324 1106 82-83 -0.227974443597585 1106 84-85 -0.08123114269036336 1106 86-87 -0.24924561696180447 1106 88-89 -0.24265343893142344 1106 90-91 -0.17561988704649423 1106 92-93 0.07699060697673232 1106 94-95 -0.12458018761303791 1106 96-97 -0.23356633208629418 1106 98-99 -0.23687946707606855 1106 100-101 -0.11312258776016293 1107 1 0.5887934106498243 1107 2 0.623996214806791 1107 3 0.42568703280539566 1107 4 0.21454433421953212 1107 5 0.14102012640967843 1107 6 0.3276621199759262 1107 7 0.27942745630574706 1107 8 0.3282744586411894 1107 9 0.41174149233961543 1107 10-11 0.45260289033682 1107 12-13 0.5460023115225212 1107 14-15 0.6613491547001402 1107 16-17 0.5721827730973743 1107 18-19 0.5083438797381561 1107 20-21 0.46490471435872394 1107 22-23 0.5903571163763672 1107 24-25 0.49817502653325363 1107 26-27 0.5375747881517583 1107 28-29 0.6060462117463956 1107 30-31 0.6522912424696088 1107 32-33 0.7278805306325253 1107 34-35 0.7033634751492812 1107 36-37 0.7575150443477199 1107 38-39 0.8080996078872218 1107 40-41 0.9496614847592184 1107 42-43 0.8979356406500258 1107 44-45 0.7900832196234973 1107 46-47 1.035261140481147 1107 48-49 1.0598563931527423 1107 50-51 0.8629910929616571 1107 52-53 0.7993336115115781 1107 54-55 0.8530767869577218 1107 56-57 0.7451896509493636 1107 58-59 0.7312367911477224 1107 60-61 0.9180256219869278 1107 62-63 0.9191360912564477 1107 64-65 0.9385348828680478 1107 66-67 0.8675422549423075 1107 68-69 0.9296699319785056 1107 70-71 0.7411955114912914 1107 72-73 0.9325800058709817 1107 74-75 1.0943071682010839 1107 76-77 1.0200561718413361 1107 78-79 1.1142864408768993 1107 80-81 1.2077179983739086 1107 82-83 1.135395722843775 1107 84-85 1.3025001050582077 1107 86-87 1.5618429529284654 1107 88-89 1.6475910327131196 1107 90-91 1.6776854575146238 1107 92-93 1.892814447174434 1107 94-95 1.8383681621113617 1107 96-97 1.702003585293408 1107 98-99 1.9370925241389898 1107 100-101 1.9035345728594315 1108 1 0.3177007546732895 1108 2 0.32419627959755815 1108 3 0.06036551637016885 1108 4 0.08774227623283082 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-1.6772236780004768 2316 74-75 -1.7734551465835864 2316 76-77 -1.7301391744827619 2316 78-79 -1.52041004496742 2316 80-81 -1.4581196009444568 2316 82-83 -1.1564083905125102 2316 84-85 -1.455369858019953 2316 86-87 -1.3204356026170885 2316 88-89 -1.4160942934529004 2316 90-91 -1.6328518141483812 2316 92-93 -1.725337521182233 2316 94-95 -2.0987015737554664 2316 96-97 -1.9641658256889336 2316 98-99 -2.2617263925894413 2316 100-101 -2.7105372896500093 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 24.0 4 45.0 5 108.0 6 247.0 7 575.0 8 1308.0 9 2254.0 10 3729.0 11 5657.0 12 7945.0 13 10716.0 14 13752.0 15 17498.0 16 21125.0 17 25199.0 18 23121.0 19 19124.0 20 19204.0 21 20653.0 22 22639.0 23 25824.0 24 31049.0 25 36969.0 26 46259.0 27 56840.0 28 70277.0 29 86468.0 30 106024.0 31 128643.0 32 158538.0 33 197930.0 34 253756.0 35 333063.0 36 451250.0 37 524271.0 38 264999.0 39 11376.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70292445599159 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22.401317742229182 27.772287926276874 27.972507238711046 21.8538870927829 20-21 23.454090797215372 25.86225871796941 27.786345132938152 22.897305351877062 22-23 22.789126536351322 26.066837223216638 28.103043637330206 23.040992603101834 24-25 23.7893960303649 26.184440556525313 27.772454678431806 22.25370873467798 26-27 23.10437306077569 25.89213698162774 28.670762879817822 22.332727077778745 28-29 22.245904733826873 27.59309606058039 27.954431305115758 22.20656790047698 30-31 22.67467454981921 26.23901853683655 27.7875624236692 23.29874448967504 32-33 22.57307967716771 26.47060687307664 28.054951794944998 22.90136165481065 34-35 23.8268311672416 26.079145238230627 27.685508793794927 22.408514800732846 36-37 23.487029081199 25.652948316599577 28.291906247389214 22.568116354812208 38-39 22.86082760456114 26.45006888533818 28.316793181568812 22.37231032853187 40-41 23.024939262316256 26.56014010398071 27.812798029045695 22.60212260465734 42-43 22.623687840881196 26.80168385970904 27.11763269684388 23.45699560256588 44-45 23.248590703185123 26.0200257304539 27.296326281499173 23.435057284861806 46-47 23.9524567783209 26.914261348978968 26.90630712926541 22.226974743434724 48-49 23.788134550210177 26.786383146769342 26.774159011155817 22.651323291864664 50-51 22.607202995417293 27.03993100428322 27.755151249963735 22.597714750335758 52-53 23.685530530323547 27.351740730710688 26.35380716889964 22.60892157006613 54-55 22.760902118209685 26.573989746579652 27.034344672155548 23.630763463055114 56-57 23.583682026509354 26.687818384718465 26.729758286090494 22.998741302681687 58-59 24.53427200422622 26.286575881196256 26.343989375752063 22.835162738825463 60-61 23.269798372094847 26.657751527088703 27.849021627634997 22.223428473181457 62-63 22.957993563126387 26.770673870628848 27.578484833324996 22.69284773291977 64-65 22.68988670233942 27.66145095924663 26.84993276125501 22.79872957715894 66-67 23.093804170463898 26.909722772293737 26.36664029102697 23.62983276621539 68-69 22.870691260884147 26.82609430088257 26.952375981567844 23.35083845666544 70-71 23.58594347058944 26.772620193658565 26.567178763892933 23.07425757185906 72-73 23.377017951202983 26.13668273935104 27.518174317366707 22.96812499207927 74-75 22.711726878646452 27.9545813820552 26.539522595250496 22.79416914404785 76-77 23.2009725081355 26.694931435008122 26.520857147859754 23.583238908996616 78-79 23.21272729850098 26.947898226306634 26.750157807094272 23.089216668098118 80-81 23.73460878841163 26.849201108829355 26.589422424390914 22.826767678368103 82-83 23.273345492339693 26.664931095257856 27.18023236421516 22.88149104818729 84-85 22.90585767325061 27.19766351469734 26.927215974632595 22.969262837419453 86-87 23.133722631876065 27.284413354668875 26.46526873857099 23.116595274884073 88-89 22.77689650398408 27.345269728161785 25.894137055033184 23.983696712820947 90-91 23.05697952190536 26.560225853832165 26.49996280470536 23.88283181955712 92-93 23.5739259910475 27.007095997322555 26.68267086182024 22.736307149809708 94-95 23.099213377895634 26.747386222964813 26.734545182867254 23.4188552162723 96-97 22.362682794144185 26.683417202753866 27.483620530647112 23.47027947245484 98-99 22.732800402383212 27.26803344877169 26.063151883986624 23.93601426485848 100-101 22.23098344628975 26.632181021120665 26.704661249161543 24.432174283428047 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 278.0 1 214.5 2 189.5 3 227.0 4 206.5 5 214.5 6 347.0 7 717.0 8 1141.5 9 1339.5 10 1301.0 11 1222.5 12 1261.5 13 1414.5 14 1665.0 15 1841.0 16 2006.5 17 2172.5 18 2383.5 19 2717.0 20 3065.5 21 3579.0 22 4175.0 23 5057.0 24 6290.0 25 7985.0 26 10489.0 27 13698.5 28 17268.0 29 21588.0 30 27190.5 31 33685.0 32 41801.0 33 47174.5 34 51267.0 35 56813.0 36 63111.5 37 69687.5 38 73799.5 39 75702.5 40 77869.5 41 83487.0 42 88553.0 43 93364.0 44 98900.5 45 104181.0 46 108626.5 47 129588.5 48 174345.0 49 171275.0 50 130862.5 51 113892.5 52 106481.0 53 104189.5 54 103194.5 55 96380.5 56 87080.0 57 78521.0 58 72470.0 59 65050.0 60 54352.0 61 45509.5 62 38160.5 63 29743.5 64 21526.0 65 16315.5 66 12406.0 67 9754.5 68 7156.0 69 4788.0 70 3498.5 71 2555.5 72 1996.5 73 1671.5 74 1332.0 75 1060.5 76 786.0 77 498.0 78 296.0 79 195.5 80 136.0 81 96.5 82 65.0 83 42.5 84 26.5 85 13.0 86 8.5 87 5.0 88 3.0 89 2.5 90 1.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0568084571356915 2 0.010705488347025909 3 0.0038019491325886404 4 0.011906103862580217 5 0.0 6 0.0 7 0.0 8 0.0 9 0.003268342236786726 10-11 2.334530169133376E-4 12-13 1.5007693944428844E-4 14-15 3.3350430987619654E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001434068532467645 24-25 0.0 26-27 5.0025646481429475E-5 28-29 0.0 30-31 0.0 32-33 0.004168803873452457 34-35 4.168803873452457E-4 36-37 0.0027180601254910017 38-39 3.3350430987619654E-5 40-41 0.00391867564104531 42-43 0.011672650845666878 44-45 0.0023178549536395656 46-47 0.0017842480578376515 48-49 0.009838377141347797 50-51 5.002564648142947E-4 52-53 0.002201128445182897 54-55 0.011355821751284492 56-57 0.004135453442464837 58-59 0.0012673163775295468 60-61 0.0063866075341291635 62-63 0.0037352482706134015 64-65 0.018759617430536057 66-67 0.00381862434808245 68-69 2.1677780141952775E-4 70-71 0.001350692454998596 72-73 0.0 74-75 0.0 76-77 9.838377141347797E-4 78-79 0.011322471320296873 80-81 0.01752565148399413 82-83 0.013673676704924058 84-85 0.009454847184990171 86-87 0.011155719165358773 88-89 0.030248840905771023 90-91 0.02571318229145475 92-93 0.0027180601254910017 94-95 0.008754488134250159 96-97 0.01163930041467926 98-99 0.010838890070976387 100-101 0.01344022368801072 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2998462.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.219348058481486 #Duplication Level Percentage of deduplicated Percentage of total 1 71.39437467943185 32.28407078046866 2 14.950967629251737 13.521460180764484 3 5.429985145336682 7.366211647180908 4 2.6526378382831712 4.798022147296986 5 1.466303564019867 3.3152645610403133 6 0.9016116313699734 2.4462174103496483 7 0.5962311087829334 1.8872827421944862 8 0.42023345198626305 1.5202146184947198 9 0.3206134280758559 1.3048137176746544 >10 1.6553018212459853 13.574250787469962 >50 0.12990200797549128 4.066299795395256 >100 0.07280881373150112 5.986924930295938 >500 0.00555054129631656 1.7613604682413437 >1k 0.002590252604884364 2.4380679850748916 >5k 6.660649555416936E-4 2.0083699228350165 >10k+ 2.2202165184723117E-4 1.721168305222664 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 28978 0.9664287891592424 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11873 0.3959696671160081 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10580 0.35284755984901595 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 8799 0.29345044226006534 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7961 0.26550278109244 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7384 0.2462595824125835 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7313 0.2438917018124625 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6270 0.2091072022923752 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5898 0.19670084196498072 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5611 0.18712926827153387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5412 0.18049253250499755 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5365 0.17892506224857943 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4938 0.16468442821686585 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4659 0.15537965797131997 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3458 0.11532579035518876 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3313 0.1104899778619839 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3096 0.10325293433767044 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3067 0.10228577183902948 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 3.3350430987619654E-5 0.0 0.0 0.0 0.0 8 3.3350430987619654E-5 0.0 0.0 0.0 6.670086197523931E-5 9 3.3350430987619654E-5 0.0 0.0 0.0 6.670086197523931E-5 10-11 3.3350430987619654E-5 0.0 0.0 0.0 6.670086197523931E-5 12-13 1.6675215493809826E-4 0.0 0.0 0.0 1.0005129296285896E-4 14-15 2.3345301691333757E-4 0.0 0.0 0.0 1.0005129296285896E-4 16-17 2.501282324071474E-4 0.0 0.0 0.0 1.0005129296285896E-4 18-19 2.6680344790095724E-4 0.0 0.0 0.0 1.0005129296285896E-4 20-21 2.6680344790095724E-4 0.0 0.0 0.0 1.0005129296285896E-4 22-23 3.001538788885769E-4 0.0 0.0 5.002564648142948E-5 1.0005129296285896E-4 24-25 4.0020517185143585E-4 0.0 0.0 8.337607746904913E-5 1.3340172395047862E-4 26-27 4.168803873452457E-4 0.0 0.0 3.001538788885769E-4 1.8342737043190808E-4 28-29 5.836325422833439E-4 0.0 0.0 7.670599127152521E-4 2.0010258592571793E-4 30-31 6.336581887647734E-4 0.0 0.0 0.001984350643763369 2.0010258592571793E-4 32-33 6.336581887647734E-4 0.0 0.0 0.004002051718514359 2.6680344790095724E-4 34-35 7.503846972214422E-4 1.6675215493809827E-5 0.0 0.0058696758538210585 3.001538788885769E-4 36-37 9.338120676533503E-4 3.3350430987619654E-5 0.0 0.008971265935669687 3.001538788885769E-4 38-39 0.0011005642225914486 5.002564648142948E-5 0.0 0.014023856230294065 3.001538788885769E-4 40-41 0.0011172394380852585 6.670086197523931E-5 0.0 0.02394560944911091 3.001538788885769E-4 42-43 0.0012339659465419271 6.670086197523931E-5 0.0 0.0337673113749649 3.001538788885769E-4 44-45 0.001334017239504786 6.670086197523931E-5 0.0 0.04535658614316273 3.168290943823867E-4 46-47 0.001417393316973835 6.670086197523931E-5 0.0 0.06900204171338506 3.6685474086381616E-4 48-49 0.001434068532467645 6.670086197523931E-5 0.0 0.08591071022410823 4.0020517185143585E-4 50-51 0.001434068532467645 6.670086197523931E-5 0.0 0.1086056785111834 4.0020517185143585E-4 52-53 0.001450743747961455 6.670086197523931E-5 0.0 0.13370187782936718 4.669060338266752E-4 54-55 0.0014674189634552646 6.670086197523931E-5 0.0 0.1664853514901973 5.002564648142948E-4 56-57 0.001550795040924314 6.670086197523931E-5 0.0 0.23308616217247374 5.336068958019145E-4 58-59 0.0016008206874057434 6.670086197523931E-5 0.0 0.29148276683179575 5.669573267895341E-4 60-61 0.0016008206874057434 6.670086197523931E-5 0.0 0.34657767882334345 5.669573267895341E-4 62-63 0.0016008206874057434 6.670086197523931E-5 0.0 0.40824262571945213 6.336581887647734E-4 64-65 0.0016008206874057434 6.670086197523931E-5 0.0 0.4702410769254371 6.336581887647734E-4 66-67 0.001734222411356222 6.670086197523931E-5 0.0 0.5633554802428712 6.336581887647734E-4 68-69 0.001734222411356222 6.670086197523931E-5 0.0 0.67833109107269 6.336581887647734E-4 70-71 0.001750897626850032 6.670086197523931E-5 0.0 0.7604398521642095 6.336581887647734E-4 72-73 0.0018175984888252712 6.670086197523931E-5 0.0 0.8523869903970769 6.336581887647734E-4 74-75 0.0018676241353067005 6.670086197523931E-5 0.0 0.9872061076645293 6.503334042585832E-4 76-77 0.0018676241353067005 6.670086197523931E-5 0.0 1.1341647818114753 6.67008619752393E-4 78-79 0.0018842993508005105 6.670086197523931E-5 0.0 1.3036683473060524 6.67008619752393E-4 80-81 0.0021177523677138477 6.670086197523931E-5 0.0 1.4743892035316772 6.67008619752393E-4 82-83 0.002134427583207658 6.670086197523931E-5 0.0 1.6679217545528342 7.337094817276323E-4 84-85 0.002234478876170517 6.670086197523931E-5 0.0 1.921168252257324 7.837351282090619E-4 86-87 0.0023845558156148053 6.670086197523931E-5 0.0 2.173747741342061 8.170855591966815E-4 88-89 0.002434581462096235 6.670086197523931E-5 0.0 2.4870583652552543 8.337607746904913E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12000 0.0 24.561764 1 TACATGG 12825 0.0 22.294725 2 ACATGGG 12650 0.0 22.000568 3 TAAGGTG 1365 0.0 21.91861 5 GTAAGGT 1220 0.0 19.853502 4 CATGGGG 6135 0.0 19.662827 4 GAGTACT 8660 0.0 19.494184 12-13 GTACTTT 8870 0.0 18.711428 14-15 AGTACTT 9185 0.0 17.47515 12-13 CATGGGA 6740 0.0 16.559027 4 GTACCTG 2410 0.0 16.133938 1 ATTCGCG 445 0.0 16.011177 80-81 AGTACAT 9725 0.0 15.921758 2 ACTTTTT 10685 0.0 15.888969 16-17 AGGTAAG 1435 0.0 15.887383 2 TACCTGG 2265 0.0 15.727369 2 TACTTTT 10045 0.0 15.695369 14-15 GTATAGG 980 0.0 15.674595 1 GGTAAGG 1485 0.0 15.351173 3 AGAGTAC 17905 0.0 15.170682 10-11 >>END_MODULE