FastQCFastQC Report
Thu 26 May 2016
SRR936581_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936581_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3021848
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT246900.817049699389248No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT154160.5101514040415004No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT118200.39115137492024743No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC105840.3502492514514297No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT72760.24077981420640615No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT70160.23217580765147686No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC60310.19957985974145623No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC57700.19094276085362336No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57660.19081039152200904No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC57650.1907772991891055No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG50560.16731483516047135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46440.15368079400419873No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA45310.14994136038609487No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA37830.12518829537422135No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT33430.11062766889664867No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA32080.10616020395466616No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC31740.10503506463594463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG120800.027.691191
TACATGG126350.025.9500682
ACATGGG124350.025.0681743
CATGGGG62950.022.7984144
GTATAGA6200.021.4586431
GAGTACT76850.020.22292712-13
GTACCTG27100.019.4620951
CATGGGA64550.019.141264
GTACTTT81150.018.94620914-15
TACCTGG25200.018.8566322
GTACTAG6950.017.7756081
AGTACTT82450.017.43712612-13
AGAGTAC161350.016.77370310-11
ATGGGGA38400.016.5825985
TAAGGTG10450.016.3706255
ACTTTTT97150.016.11767816-17
TACTAGC4501.3460522E-1015.839572
GTATTAC3905.502443E-915.8385221
AGTACAT93550.015.3908822
ATGGGGG33200.015.3152735
Produced by FastQC (version 0.11.5)