##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936581_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3021848 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.419132266083537 31.0 31.0 34.0 30.0 34.0 2 31.4605364002425 31.0 31.0 34.0 28.0 34.0 3 31.527684714783803 31.0 31.0 34.0 28.0 34.0 4 35.15286208968816 37.0 35.0 37.0 32.0 37.0 5 35.13039967595988 37.0 35.0 37.0 32.0 37.0 6 35.25855900098218 37.0 35.0 37.0 32.0 37.0 7 35.12532529763244 37.0 35.0 37.0 32.0 37.0 8 35.074453777953096 37.0 35.0 37.0 32.0 37.0 9 36.55594258877349 39.0 37.0 39.0 32.0 39.0 10-11 36.46093863754895 39.0 37.0 39.0 32.0 39.0 12-13 36.36073919005854 39.0 35.0 39.0 32.0 39.0 14-15 37.41540805493857 40.0 37.0 41.0 32.0 41.0 16-17 37.30373797755546 39.0 37.0 41.0 32.0 41.0 18-19 37.23501876997122 39.0 36.5 41.0 31.5 41.0 20-21 37.12736742549592 39.0 36.0 41.0 31.0 41.0 22-23 36.98981451085561 39.0 36.0 41.0 31.0 41.0 24-25 37.123740174886365 39.0 36.0 41.0 31.0 41.0 26-27 37.10622853962211 39.0 36.0 41.0 31.0 41.0 28-29 37.022549612025486 39.0 36.0 41.0 31.0 41.0 30-31 36.87384143742504 39.0 36.0 41.0 30.0 41.0 32-33 36.71820902308786 39.0 36.0 41.0 30.0 41.0 34-35 36.48647880369893 39.0 35.0 40.0 30.0 41.0 36-37 36.29830818757264 39.0 35.0 40.0 30.0 41.0 38-39 36.09946512862328 38.0 35.0 40.0 29.5 41.0 40-41 35.95786022328059 38.0 35.0 40.0 29.0 41.0 42-43 35.73986299112332 38.0 35.0 40.0 28.0 41.0 44-45 35.5055469368413 38.0 34.5 40.0 27.0 41.0 46-47 35.26566508308822 38.0 34.0 40.0 27.0 41.0 48-49 35.20128510765598 38.0 34.0 40.0 26.0 41.0 50-51 34.271441846181546 37.5 33.0 39.5 25.0 40.5 52-53 34.445612750872975 37.5 33.5 39.5 26.0 40.5 54-55 34.88656990689141 38.0 34.0 40.0 26.0 41.0 56-57 34.76734849006303 38.0 34.0 40.0 26.0 41.0 58-59 34.49335406678298 38.0 34.0 40.0 25.5 41.0 60-61 34.125667637816335 37.0 33.0 40.0 24.0 41.0 62-63 33.81208601491538 37.0 33.0 40.0 23.0 41.0 64-65 33.46970214914847 36.0 32.5 39.0 23.0 41.0 66-67 33.04773602113673 36.0 32.0 39.0 22.0 41.0 68-69 32.62444173234392 35.0 32.0 39.0 21.5 40.0 70-71 32.13532877894586 35.0 31.0 38.0 20.0 40.0 72-73 31.72963679840945 35.0 31.0 37.0 19.5 39.5 74-75 31.312635016718247 35.0 31.0 37.0 18.0 39.0 76-77 30.88192225419677 34.5 30.0 36.0 17.0 39.0 78-79 30.42151375582094 34.0 30.0 36.0 12.0 37.5 80-81 29.9081057352984 34.0 29.0 35.0 7.5 37.0 82-83 29.416978782519834 34.0 29.0 35.0 7.0 37.0 84-85 29.099517083585937 34.0 29.0 35.0 3.5 36.0 86-87 28.818332523674258 34.0 29.0 35.0 2.0 36.0 88-89 28.403242486054893 34.0 28.5 35.0 2.0 35.5 90-91 27.96329381888169 33.0 27.0 35.0 2.0 35.0 92-93 27.570695812628564 33.0 26.5 35.0 2.0 35.0 94-95 27.075225822079734 33.0 25.5 35.0 2.0 35.0 96-97 26.45745666228083 33.0 25.0 35.0 2.0 35.0 98-99 25.726512220336694 32.5 23.5 35.0 2.0 35.0 100-101 23.214813749731952 29.5 10.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37588799689962826 1101 2 0.43756895814414065 1101 3 0.4543068149749523 1101 4 0.4399340077403977 1101 5 0.36627317159125283 1101 6 0.23458308086760837 1101 7 0.34990190011870936 1101 8 0.45655573488838996 1101 9 0.6388389764131688 1101 10-11 0.62924726070856 1101 12-13 0.7208217085837063 1101 14-15 0.799405914797326 1101 16-17 0.8661335259843028 1101 18-19 0.9247190375777024 1101 20-21 0.7691708540280686 1101 22-23 0.8359224679451458 1101 24-25 0.81780129035171 1101 26-27 0.6883162334877468 1101 28-29 0.6464853644726958 1101 30-31 0.612669643749058 1101 32-33 0.5474047522562273 1101 34-35 0.514481922671429 1101 36-37 0.5212065678022739 1101 38-39 0.42845164990554707 1101 40-41 0.5053969195336094 1101 42-43 0.42777813424903144 1101 44-45 0.5154604276771622 1101 46-47 0.4602904069350586 1101 48-49 0.4989667809423963 1101 50-51 0.6188002264244687 1101 52-53 0.5872709472861501 1101 54-55 0.6928117689778617 1101 56-57 0.5877756607325537 1101 58-59 0.5599577533714211 1101 60-61 0.4665460239299577 1101 62-63 0.395647217623349 1101 64-65 0.4312983063037379 1101 66-67 0.396703952186364 1101 68-69 0.4418104014216624 1101 70-71 0.4042306241667646 1101 72-73 0.39307966866526556 1101 74-75 0.4428855745212381 1101 76-77 0.43145223844058833 1101 78-79 0.30573858341792715 1101 80-81 0.4317867791112384 1101 82-83 0.4911454643319786 1101 84-85 0.5612558778293568 1101 86-87 0.3344381188801897 1101 88-89 0.3348003528622847 1101 90-91 0.15312779435162582 1101 92-93 0.09257016724570732 1101 94-95 0.207058640032308 1101 96-97 0.1520843663717244 1101 98-99 0.23913423047948967 1101 100-101 0.8142672794886003 1102 1 0.2804470769090237 1102 2 0.3428955121923174 1102 3 0.2896027055889583 1102 4 0.3689640995142298 1102 5 0.3217724837233362 1102 6 0.28786456735964805 1102 7 0.2341032954193949 1102 8 0.2556811168800266 1102 9 0.42472578237855885 1102 10-11 0.40042564856031504 1102 12-13 0.3559287422531696 1102 14-15 0.3508490659074184 1102 16-17 0.3706906148079625 1102 18-19 0.520835308283452 1102 20-21 0.5491154577535937 1102 22-23 0.3607180181985896 1102 24-25 0.27963699340262593 1102 26-27 0.2844294471863478 1102 28-29 0.3328587385683335 1102 30-31 0.16628273289526874 1102 32-33 0.10536916059603385 1102 34-35 -0.07639348107039723 1102 36-37 0.07802294602379334 1102 38-39 0.009837194607626998 1102 40-41 -0.0937135657447925 1102 42-43 0.11867914249130251 1102 44-45 -0.18428590682668045 1102 46-47 0.07352690915374183 1102 48-49 0.1200075332437649 1102 50-51 0.14050340738913292 1102 52-53 0.013667262154918092 1102 54-55 0.12323730189476834 1102 56-57 0.17187756284371147 1102 58-59 0.1838908475476515 1102 60-61 0.1404803946132276 1102 62-63 -0.012663264581526334 1102 64-65 0.026845891057405424 1102 66-67 0.04397993986613358 1102 68-69 -0.10514213011487783 1102 70-71 -0.04765807334245409 1102 72-73 -0.1730057617956291 1102 74-75 -0.36341980843046073 1102 76-77 -0.15968239124554628 1102 78-79 -0.3160479546958044 1102 80-81 -0.10768682573885968 1102 82-83 -0.13220436114183087 1102 84-85 -0.1282294469251859 1102 86-87 -0.1112290733438428 1102 88-89 -0.22683035720363875 1102 90-91 -0.1079342545487556 1102 92-93 -0.27391306246747504 1102 94-95 -0.13342416550047886 1102 96-97 -0.29609101759870526 1102 98-99 -0.1253145609740436 1102 100-101 -0.06364324951326594 1103 1 0.28160468725389975 1103 2 0.3947606327148847 1103 3 0.3217763978269019 1103 4 0.4202831462700303 1103 5 0.3928212623507221 1103 6 0.3109504859374468 1103 7 0.38018706734531094 1103 8 0.269163153983655 1103 9 0.43599355846239973 1103 10-11 0.4894899084409019 1103 12-13 0.4776865582486849 1103 14-15 0.3582923856792988 1103 16-17 0.6245037203802397 1103 18-19 0.5757172903091714 1103 20-21 0.6397552828514961 1103 22-23 0.5585019740807766 1103 24-25 0.6062989398126106 1103 26-27 0.5200280001692832 1103 28-29 0.3031851396128289 1103 30-31 0.1817496635264888 1103 32-33 0.33259610967900954 1103 34-35 0.2641059250588995 1103 36-37 0.3401457423705594 1103 38-39 0.38208882969199465 1103 40-41 0.21910600917124157 1103 42-43 0.1983006601256463 1103 44-45 0.2504655837937335 1103 46-47 0.1736573559815895 1103 48-49 0.2474322576147827 1103 50-51 0.3403227779093072 1103 52-53 0.4073238331847904 1103 54-55 0.36834959084117 1103 56-57 0.4188574405826202 1103 58-59 0.3031096475424988 1103 60-61 0.18302461792050195 1103 62-63 0.28857801928867133 1103 64-65 0.36386755437745677 1103 66-67 0.13664952486988113 1103 68-69 0.3652229872707551 1103 70-71 0.3875039012999082 1103 72-73 0.4195151078807129 1103 74-75 0.2899391272185241 1103 76-77 0.32297483424733997 1103 78-79 0.44304431798478916 1103 80-81 0.3503621199714182 1103 82-83 0.21463775576533806 1103 84-85 0.29856220335514294 1103 86-87 0.4241296777214778 1103 88-89 0.5912277698103949 1103 90-91 0.7230858805902614 1103 92-93 0.6292753644339264 1103 94-95 0.7075870557994861 1103 96-97 0.7356705808760253 1103 98-99 0.882702652195654 1103 100-101 1.2560329563975685 1104 1 0.08862590628494615 1104 2 0.21530517633005175 1104 3 0.14528754747736983 1104 4 0.25707042361035093 1104 5 0.3339545822536749 1104 6 0.2293295263658166 1104 7 0.21950859945998502 1104 8 0.2336023235967346 1104 9 0.35316727072715537 1104 10-11 0.35681524777703544 1104 12-13 0.15854721705759545 1104 14-15 0.3344786388069707 1104 16-17 0.16412334859222 1104 18-19 0.4373224302527774 1104 20-21 0.610754415715931 1104 22-23 0.2536417814423828 1104 24-25 0.25254303117424826 1104 26-27 -0.08562178703700596 1104 28-29 0.17926321976885617 1104 30-31 0.2720250690899775 1104 32-33 0.05177605831266874 1104 34-35 -0.14195584886104484 1104 36-37 -0.07103405346816771 1104 38-39 0.17495814817794297 1104 40-41 0.2643348901381515 1104 42-43 0.19818460410343874 1104 44-45 0.23704979959078543 1104 46-47 0.23035084448450505 1104 48-49 0.07939165309186791 1104 50-51 7.875856566386119E-5 1104 52-53 0.19170184103085575 1104 54-55 0.2708896605818367 1104 56-57 0.18659357444266078 1104 58-59 0.1394564229368669 1104 60-61 0.18099108283946208 1104 62-63 0.34299953476134704 1104 64-65 0.11989796640796868 1104 66-67 0.3530786781426656 1104 68-69 0.25115463732949195 1104 70-71 0.3718783082463375 1104 72-73 0.33725292053855327 1104 74-75 0.0012979461623707778 1104 76-77 0.28666511405670647 1104 78-79 0.2487016962768145 1104 80-81 0.3314784426548485 1104 82-83 0.46738198191740565 1104 84-85 0.4856205457150544 1104 86-87 0.5217337656184604 1104 88-89 0.46669600841573455 1104 90-91 0.43013456864627386 1104 92-93 0.5295409064520449 1104 94-95 0.45311731746810224 1104 96-97 0.6142375396048472 1104 98-99 0.891293109227675 1104 100-101 1.2693905909373981 1105 1 0.24106903124496526 1105 2 0.3093024490669656 1105 3 0.18086114870225956 1105 4 0.2557939879798994 1105 5 0.3330446105453362 1105 6 0.29063910126445336 1105 7 0.39175331313754214 1105 8 0.4630709412658902 1105 9 0.4705863296156636 1105 10-11 0.3938950478677441 1105 12-13 0.27776665051080585 1105 14-15 0.15417364760050134 1105 16-17 0.31926492582079646 1105 18-19 0.2510533592613271 1105 20-21 0.3671493718865264 1105 22-23 0.1489821765997874 1105 24-25 0.3496006393027997 1105 26-27 0.2993465138337328 1105 28-29 0.14292374109271577 1105 30-31 0.13498897946575994 1105 32-33 0.1022749475090805 1105 34-35 0.13442569601583898 1105 36-37 0.2105033816251236 1105 38-39 0.023139136994046794 1105 40-41 -0.08550121369457742 1105 42-43 0.14800733521760634 1105 44-45 -0.17803842016927973 1105 46-47 -0.04252755442819023 1105 48-49 0.24707031470132534 1105 50-51 0.3286456901471695 1105 52-53 0.0791706554332805 1105 54-55 0.14052578601666 1105 56-57 0.2749034637287693 1105 58-59 0.35323475919709324 1105 60-61 0.10710729497835558 1105 62-63 0.16891647410610489 1105 64-65 0.2531497534821412 1105 66-67 0.2741299731265059 1105 68-69 0.29060781368767863 1105 70-71 0.4489392820567062 1105 72-73 0.3706089328453146 1105 74-75 0.3454475505581378 1105 76-77 0.07039393911229652 1105 78-79 0.2878145273038335 1105 80-81 0.14738251879447972 1105 82-83 0.2740841570093693 1105 84-85 0.31457399226001925 1105 86-87 0.5308155613302539 1105 88-89 0.3410488299146941 1105 90-91 0.2696409706745513 1105 92-93 0.3263111056058996 1105 94-95 0.3097433709313293 1105 96-97 0.5733771337492684 1105 98-99 0.5901494122946254 1105 100-101 1.0214750955890786 1106 1 0.1527626280934662 1106 2 0.182501687899844 1106 3 0.2389992938581642 1106 4 0.2587822366278232 1106 5 0.1319763058114063 1106 6 0.12261513117698541 1106 7 0.24311106168531182 1106 8 0.1653170519689624 1106 9 0.43383130524495783 1106 10-11 0.25791471135175925 1106 12-13 0.46783024802699913 1106 14-15 0.5154148841632349 1106 16-17 0.5107064695211321 1106 18-19 0.5336460013124693 1106 20-21 0.3345440437166687 1106 22-23 0.2467519235450979 1106 24-25 0.17269271381705664 1106 26-27 0.05865578805420313 1106 28-29 0.31590598052651586 1106 30-31 0.29509697839745286 1106 32-33 0.04259577277449722 1106 34-35 0.017261878161789923 1106 36-37 0.012435177044771706 1106 38-39 0.1480112742712052 1106 40-41 0.07846353224920222 1106 42-43 -0.12708998066674582 1106 44-45 0.20940332383820248 1106 46-47 0.06383086181004671 1106 48-49 0.0790071177994136 1106 50-51 -0.02032239424890747 1106 52-53 0.19456542741698257 1106 54-55 0.3759442610116466 1106 56-57 0.26471250425758086 1106 58-59 0.18655668615175358 1106 60-61 0.2642470759758808 1106 62-63 0.39504153252901375 1106 64-65 0.2912167896041993 1106 66-67 0.36859565383693393 1106 68-69 0.377197584669851 1106 70-71 0.4430016777300665 1106 72-73 0.432950523283985 1106 74-75 0.26890718866991037 1106 76-77 0.46301846338284136 1106 78-79 0.6100547957153992 1106 80-81 0.6565717756549958 1106 82-83 0.7397021045288952 1106 84-85 0.4902795741522574 1106 86-87 0.6087949630796068 1106 88-89 0.8350881902672391 1106 90-91 0.7719872349464367 1106 92-93 0.795998125703381 1106 94-95 0.5936542008705707 1106 96-97 0.6048694219244695 1106 98-99 1.0743947551536763 1106 100-101 1.441083828980279 1107 1 0.09228087776818938 1107 2 0.10460365324943055 1107 3 0.0536664855311102 1107 4 -0.014770800419356078 1107 5 0.13806067203752548 1107 6 0.13516768887060948 1107 7 0.2256239015127477 1107 8 0.200819346018136 1107 9 0.24970612548737137 1107 10-11 0.19591700540092916 1107 12-13 0.16872441360941792 1107 14-15 0.11649240248138426 1107 16-17 0.16613138221030255 1107 18-19 0.3367628266223335 1107 20-21 0.434403319208009 1107 22-23 0.46901087718476475 1107 24-25 0.4437722865076026 1107 26-27 0.1406457901085716 1107 28-29 0.30564644481466985 1107 30-31 0.38623835345737234 1107 32-33 0.2129484400346442 1107 34-35 0.23718158644091147 1107 36-37 0.25869330893547726 1107 38-39 0.2658250796536592 1107 40-41 0.23275203461172822 1107 42-43 0.3143975561407544 1107 44-45 0.41972757228030133 1107 46-47 0.3307695045546808 1107 48-49 0.38716075061459065 1107 50-51 0.1920435579527009 1107 52-53 0.44242151863112156 1107 54-55 0.44277898675293414 1107 56-57 0.41904346364948 1107 58-59 0.44018864533823177 1107 60-61 0.42201825313126307 1107 62-63 0.5121527425543562 1107 64-65 0.42900046419160986 1107 66-67 0.43748132801347595 1107 68-69 0.5519668101714643 1107 70-71 0.5903205505644209 1107 72-73 0.5151144622006427 1107 74-75 0.4005123382966964 1107 76-77 0.3421588797356456 1107 78-79 0.48947974264411087 1107 80-81 0.6895720153317768 1107 82-83 0.7162076170948097 1107 84-85 0.5767578662840087 1107 86-87 0.43026127137241943 1107 88-89 0.33183560518198263 1107 90-91 0.3596603158202285 1107 92-93 0.4068162795073924 1107 94-95 0.47424945527035334 1107 96-97 0.3858055309431627 1107 98-99 0.44343484965480684 1107 100-101 0.8824934708346923 1108 1 0.008373863108797508 1108 2 0.08557389308523611 1108 3 0.018087668544534807 1108 4 0.18343236208085045 1108 5 0.24504781403555143 1108 6 0.008252850719777882 1108 7 -0.13206945127816283 1108 8 -0.0064811080533360155 1108 9 0.051061581241611975 1108 10-11 -0.1925149652582263 1108 12-13 0.1676678560559921 1108 14-15 0.10179870072303032 1108 16-17 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-2.6430340655123423 2316 70-71 -2.5291703680794058 2316 72-73 -2.1359953382464667 2316 74-75 -1.5541094693207214 2316 76-77 -1.541334738074731 2316 78-79 -2.5130854957994373 2316 80-81 -1.9374456631789414 2316 82-83 -1.5757742098906746 2316 84-85 -1.4165410921673676 2316 86-87 -2.3404723456137866 2316 88-89 -2.4342025437254726 2316 90-91 -2.593978407947887 2316 92-93 -3.005736647852057 2316 94-95 -3.4286766164120976 2316 96-97 -4.543974929975583 2316 98-99 -5.776868909604264 2316 100-101 -5.831620491686042 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5716.0 3 2343.0 4 2300.0 5 2645.0 6 2927.0 7 3517.0 8 4290.0 9 5405.0 10 6878.0 11 8649.0 12 10689.0 13 12759.0 14 14160.0 15 17146.0 16 20140.0 17 24045.0 18 20999.0 19 18037.0 20 18770.0 21 20756.0 22 23551.0 23 27020.0 24 31120.0 25 35860.0 26 42618.0 27 50856.0 28 60857.0 29 73456.0 30 89421.0 31 111310.0 32 139483.0 33 177814.0 34 233936.0 35 316037.0 36 429864.0 37 530768.0 38 377543.0 39 48157.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.418150099392065 17.74908842334894 13.575912859396233 23.25684861786276 2 15.48332621138966 19.946318970176875 39.16053964362919 25.409815174804272 3 20.84226455662517 23.155456905716733 29.302245835810243 26.700032701847853 4 12.665441663355848 15.451297841345518 37.1185604555688 34.76470003972983 5 13.671747862159755 37.5254327415113 34.04793887599616 14.754880520332783 6 32.843203470770995 35.19827017663745 16.96775022314748 14.990776129444077 7 29.55530584352635 32.15488973529053 20.042955030645274 18.246849390537843 8 27.02156186995342 32.873889711018634 19.416045103472477 20.68850331555547 9 26.06976568645367 17.167830686149095 20.591822465178737 36.1705811622185 10-11 25.62925459207483 25.387146047654575 26.99689885855547 21.986700501715124 12-13 27.187940631473424 22.88285836591875 27.172496286239173 22.756704716368656 14-15 23.23922097011269 24.688894553544234 26.198902233934078 25.872982242408995 16-17 22.590589210607167 28.097868263251012 26.60072280668298 22.710819719458836 18-19 22.364686961725557 27.55450306793153 28.03692940322462 22.043880567118297 20-21 23.418370876081298 25.716410021681362 27.653534399761178 23.211684702476163 22-23 22.757423318345722 25.820171527006742 28.05803579186062 23.364369362786917 24-25 23.556409827458367 25.916804793630295 27.79928625714897 22.727499121762374 26-27 22.94397395743095 25.66944490916724 28.71958747839866 22.666993655003147 28-29 22.171654142131388 27.284689355452237 28.00457296944172 22.539083532974658 30-31 22.51375602084468 26.027606032063954 27.829027537194044 23.62961040989732 32-33 22.432708361256033 26.284204273372747 28.099320423531516 23.183766941839703 34-35 23.80539799217209 25.911798739982 27.610455107990788 22.672348159855122 36-37 23.31429252245514 25.451270527340004 28.412717849601865 22.821719100602998 38-39 22.7839194682671 26.208805047265994 28.23086879868622 22.776406685780685 40-41 22.85245074314458 26.392656664308788 27.98984045039567 22.765052142150964 42-43 22.421111519810417 26.487967445693872 27.20439912680649 23.88652190768922 44-45 22.958810025484986 25.887879292297683 27.392936412623158 23.76037426959417 46-47 23.737840792458755 26.56023135607481 27.131208829865162 22.570719021601274 48-49 23.434123250822726 26.476600888837115 27.016475371494636 23.072800488845523 50-51 22.390118595060123 26.78645029721155 27.98520767579329 22.838223431935038 52-53 23.318759026208603 27.06164892201753 26.594801168592035 23.02479088318183 54-55 22.44814716450684 26.25846287751487 27.34558325287239 23.947806705105897 56-57 23.299302025280134 26.344842902318454 26.921912785347246 23.433942287054162 58-59 24.13343687847473 26.02907085555635 26.635663799538552 23.201828466430374 60-61 23.079827837560998 26.27232907450278 28.107354963404724 22.540488124531493 62-63 22.716955593301392 26.573065082334367 27.760801344288634 22.949177980075604 64-65 22.411856060217474 27.379265065372273 27.11611474704047 23.092764127369783 66-67 22.718114530709368 26.7230985873542 26.529627172044272 24.029159709892163 68-69 22.492241863223782 26.539695579185953 27.28977567599055 23.67828688159971 70-71 23.305593287452744 26.427312315365814 26.79063615013414 23.4764582470473 72-73 23.03386022293736 25.95792130526587 27.772077514970018 23.236140956826755 74-75 22.372556647327396 27.631621402802253 26.930751425211497 23.06507052465885 76-77 22.838509765908434 26.398523725125216 26.8558552014216 23.907111307544746 78-79 22.91221066977046 26.63798943434391 27.09594705191852 23.353852843967108 80-81 23.42232023668651 26.54305348960856 26.822794066421878 23.211832207283052 82-83 22.989173718082473 26.31504485344136 27.56191972364992 23.13386170482625 84-85 22.664456405933347 26.863372590450073 27.148492253330698 23.323678750285882 86-87 22.90515753575394 26.98099907502246 26.902913761977963 23.210929627245637 88-89 22.55012210131414 27.01963582401923 26.175086566340276 24.255155508326354 90-91 22.68864638575648 26.263160942838944 26.876204816785116 24.171987854619463 92-93 23.348123038030742 26.635717012727884 27.06173398489454 22.954425964346832 94-95 22.858018953708555 26.286211292033695 27.160804356880703 23.69496539737705 96-97 22.10506806903045 26.296108142634694 27.865096398720528 23.733727389614323 98-99 22.534756970550223 26.837476874059675 26.51135125992622 24.11641489546388 100-101 22.21056778287763 25.971808652386713 27.163204266440676 24.65441929829498 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 176.0 1 130.0 2 127.5 3 193.0 4 184.0 5 188.0 6 345.5 7 666.5 8 1065.0 9 1301.5 10 1239.0 11 1193.0 12 1272.5 13 1394.0 14 1564.5 15 1679.0 16 1824.5 17 2000.5 18 2181.0 19 2449.0 20 2827.0 21 3344.0 22 4018.0 23 4876.5 24 5952.5 25 7593.5 26 10163.0 27 13415.5 28 16821.0 29 20941.5 30 26735.5 31 33607.5 32 42028.5 33 47576.5 34 51322.0 35 56468.0 36 62899.5 37 69567.5 38 73550.0 39 75884.5 40 78602.5 41 84312.5 42 89310.5 43 94072.5 44 99877.5 45 105834.5 46 110853.0 47 130732.0 48 173498.5 49 171755.5 50 132959.5 51 115359.5 52 107751.0 53 105915.0 54 104417.5 55 97555.5 56 88826.5 57 80091.0 58 73506.5 59 65547.5 60 55015.5 61 46343.5 62 38877.5 63 30452.5 64 22362.0 65 17060.5 66 12957.0 67 9999.5 68 7402.0 69 5067.5 70 3777.0 71 2726.5 72 2015.0 73 1723.5 74 1369.0 75 1047.5 76 778.0 77 490.0 78 315.0 79 199.5 80 120.0 81 79.5 82 55.5 83 37.5 84 31.0 85 21.0 86 16.5 87 12.5 88 5.5 89 4.5 90 4.5 91 4.5 92 4.5 93 5.0 94 4.5 95 2.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03252976324421347 2 0.022138770712491162 3 0.020252507736987432 4 0.04791769804437549 5 0.05384122563411528 6 0.04712348205468971 7 0.04897665269728987 8 0.0409683081346249 9 0.04235818611657502 10-11 0.049919784185041734 12-13 0.04384734109723586 14-15 0.03865184483137471 16-17 0.046329266065003934 18-19 0.049324122192777396 20-21 0.01188014751238315 22-23 0.0190115452531034 24-25 0.005675335092962981 26-27 0.011036293023342008 28-29 0.005923527589739788 30-31 0.00867019122073645 32-33 0.0037394336181038887 34-35 0.007429228736852415 36-37 0.008868745218157895 38-39 0.010953562191083073 40-41 0.0020020861406662414 42-43 0.002200640138087687 44-45 0.0022833709703466223 46-47 4.136541612946779E-4 48-49 0.0011416854851733112 50-51 3.4746949548752947E-4 52-53 0.00304449462712883 54-55 0.0064695510826487624 56-57 0.015338296300806657 58-59 0.009911153704620484 60-61 0.009365130211711509 62-63 0.005807704424577278 64-65 3.3092332903574235E-5 66-67 0.0070486669084613126 68-69 0.016678535783401414 70-71 0.005410596429734388 72-73 0.0019028091419555186 74-75 0.0034250564555199335 76-77 0.006237904752323744 78-79 0.005658788926511195 80-81 0.00987806137171691 82-83 0.00574151975877013 84-85 0.016049781458233506 86-87 0.005063126934246858 88-89 0.00585734292393264 90-91 0.00407035694713963 92-93 0.01619869695629959 94-95 0.010870831358824136 96-97 0.009365130211711509 98-99 0.02050070023376424 100-101 0.03517714987649941 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3021848.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.63511791756319 #Duplication Level Percentage of deduplicated Percentage of total 1 72.90666999591404 34.00011152236318 2 14.072348484459054 13.125312618995794 3 5.277964338227392 7.3841546783378345 4 2.4673449814886057 4.602596966201156 5 1.391823685608937 3.2453930849415085 6 0.8474567584794991 2.371274751703639 7 0.5728402062840463 1.8700129397584406 8 0.3976327122524238 1.4834918739017804 9 0.30714190680303344 1.2891239137066202 >10 1.5607231485879751 13.217920802552932 >50 0.1208760987957257 3.897843309476884 >100 0.068625935814466 5.860928796246653 >500 0.004917254688916229 1.5479213119683164 >1k 0.002850582428205139 2.607996159162407 >5k 4.988519249358994E-4 1.4184404326717663 >10k+ 2.850582428205139E-4 2.0774768380110618 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24690 0.817049699389248 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15416 0.5101514040415004 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11820 0.39115137492024743 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10584 0.3502492514514297 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7276 0.24077981420640615 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7016 0.23217580765147686 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6031 0.19957985974145623 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5770 0.19094276085362336 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5766 0.19081039152200904 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5765 0.1907772991891055 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5056 0.16731483516047135 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4644 0.15368079400419873 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4531 0.14994136038609487 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3783 0.12518829537422135 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3343 0.11062766889664867 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3208 0.10616020395466616 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3174 0.10503506463594463 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.3092332903574235E-5 0.0 0.0 0.0 0.0 6 3.3092332903574235E-5 0.0 0.0 0.0 0.0 7 1.3236933161429694E-4 0.0 0.0 0.0 0.0 8 1.3236933161429694E-4 0.0 0.0 0.0 3.3092332903574235E-5 9 1.3236933161429694E-4 0.0 0.0 0.0 6.618466580714847E-5 10-11 1.3236933161429694E-4 0.0 0.0 0.0 6.618466580714847E-5 12-13 1.3236933161429694E-4 0.0 0.0 0.0 1.3236933161429694E-4 14-15 1.3236933161429694E-4 0.0 0.0 0.0 1.3236933161429694E-4 16-17 1.6546166451787117E-4 0.0 0.0 0.0 1.3236933161429694E-4 18-19 1.9855399742144543E-4 0.0 0.0 0.0 1.3236933161429694E-4 20-21 2.3164633032501965E-4 0.0 0.0 4.9638499355361356E-5 1.3236933161429694E-4 22-23 2.3164633032501965E-4 0.0 0.0 1.1582316516250983E-4 1.4891549806608404E-4 24-25 3.9710799484289085E-4 0.0 0.0 1.9855399742144543E-4 1.6546166451787117E-4 26-27 3.9710799484289085E-4 0.0 0.0 3.9710799484289085E-4 1.820078309696583E-4 28-29 5.956619922643363E-4 0.0 0.0 9.596776542036529E-4 1.9855399742144543E-4 30-31 6.287543251679105E-4 0.0 0.0 0.0022337324709912607 2.3164633032501965E-4 32-33 6.287543251679105E-4 0.0 0.0 0.004169633945850354 3.3092332903574234E-4 34-35 7.942159896857817E-4 0.0 0.0 0.006204812419420169 4.632926606500393E-4 36-37 0.0010258623200108013 0.0 0.0 0.009447861043970444 4.632926606500393E-4 38-39 0.0010920469858179498 0.0 0.0 0.015090103804029852 4.632926606500393E-4 40-41 0.0010920469858179498 0.0 0.0 0.026176035326727222 4.632926606500393E-4 42-43 0.001108593152269737 0.0 0.0 0.03770871334362284 4.798388271018264E-4 44-45 0.0011582316516250983 0.0 0.0 0.052037693490870486 4.963849935536135E-4 46-47 0.0013236933161429694 0.0 0.0 0.07690658166790654 5.294773264571878E-4 48-49 0.0014229703148536921 0.0 0.0 0.09573611909004026 5.62569659360762E-4 50-51 0.0014229703148536921 0.0 0.0 0.11992661444255304 5.62569659360762E-4 52-53 0.0014229703148536921 0.0 0.0 0.14780690491381432 5.62569659360762E-4 54-55 0.0014229703148536921 0.0 0.0 0.18351353211677093 5.956619922643363E-4 56-57 0.0014891549806608406 0.0 0.0 0.251518276233616 5.956619922643363E-4 58-59 0.0014891549806608406 0.0 0.0 0.3144268010833106 5.956619922643363E-4 60-61 0.0014891549806608406 0.0 0.0 0.37113051351358506 5.956619922643363E-4 62-63 0.0014891549806608406 0.0 0.0 0.4356771088420066 5.956619922643363E-4 64-65 0.001522247313564415 0.0 0.0 0.503119283299491 5.956619922643363E-4 66-67 0.0015884319793715634 0.0 0.0 0.5986733945585615 5.956619922643363E-4 68-69 0.0015884319793715634 0.0 0.0 0.7199237023172576 5.956619922643363E-4 70-71 0.0016049781458233505 0.0 0.0 0.8095542859865883 6.287543251679105E-4 72-73 0.0016380704787269248 0.0 0.0 0.9052738589101768 7.445774903304203E-4 74-75 0.001654616645178712 0.0 0.0 1.0403733079890185 7.611236567822074E-4 76-77 0.001654616645178712 0.0 0.0 1.1926807701777191 7.611236567822074E-4 78-79 0.001654616645178712 0.0 0.0 1.3707009750324968 7.611236567822074E-4 80-81 0.0019193553084073057 0.0 0.0 1.5518153130137584 7.776698232339946E-4 82-83 0.00195244764131088 0.0 0.0 1.7533641665629773 8.769468219447172E-4 84-85 0.002002086140666241 0.0 0.0 2.011732555707633 9.100391548482915E-4 86-87 0.0020351784735698156 0.0 0.0 2.2680326740458154 9.431314877518657E-4 88-89 0.0020517246400216026 0.0 0.0 2.5812019664787904 9.596776542036529E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12080 0.0 27.69119 1 TACATGG 12635 0.0 25.950068 2 ACATGGG 12435 0.0 25.068174 3 CATGGGG 6295 0.0 22.798414 4 GTATAGA 620 0.0 21.458643 1 GAGTACT 7685 0.0 20.222927 12-13 GTACCTG 2710 0.0 19.462095 1 CATGGGA 6455 0.0 19.14126 4 GTACTTT 8115 0.0 18.946209 14-15 TACCTGG 2520 0.0 18.856632 2 GTACTAG 695 0.0 17.775608 1 AGTACTT 8245 0.0 17.437126 12-13 AGAGTAC 16135 0.0 16.773703 10-11 ATGGGGA 3840 0.0 16.582598 5 TAAGGTG 1045 0.0 16.370625 5 ACTTTTT 9715 0.0 16.117678 16-17 TACTAGC 450 1.3460522E-10 15.83957 2 GTATTAC 390 5.502443E-9 15.838522 1 AGTACAT 9355 0.0 15.390882 2 ATGGGGG 3320 0.0 15.315273 5 >>END_MODULE