FastQCFastQC Report
Thu 26 May 2016
SRR936581_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936581_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3021848
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT291790.9656011817933926No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT120760.39962301214356244No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT107710.3564375177043981No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC90130.29826119645991456No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT81410.26940468216799784No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC75330.24928454376262474No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC74060.24508181748387078No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT65980.21834321249778282No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61690.2041466016821495No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG58870.19481456380334153No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA56120.18571417225485862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54940.18180927697223687No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC51610.17078953011534664No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA46980.15546777998099176No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC35710.1181727207986636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34400.11383762518829538No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT31750.1050681569688482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG122400.024.3039991
TACATGG128750.022.761452
ACATGGG126200.022.1679293
CATGGGG66000.020.2213444
TACCTGG22450.020.0987592
GAGTACT85950.020.06161312-13
GGACCGT3951.8189894E-1219.2391056
GTACCTG24050.018.968851
GTACTTT90550.018.83232314-15
AGTACTT90750.018.03215812-13
TAAGGTG13050.017.1054675
AGACCCG8250.016.6952025
ATGGGGA37400.016.5089455
AGTACAT98700.016.265282
ACTTTTT106850.016.04676416-17
AGAGTAC178600.016.03818910-11
TACTTTT101400.015.97402514-15
GTAGGAC6850.015.9480093
TAATACC6850.015.9472164
GTAAGGT12550.015.89473154
Produced by FastQC (version 0.11.5)