FastQCFastQC Report
Thu 26 May 2016
SRR936580_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936580_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054080
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT246180.8060692581726739No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT153560.5028028080469404No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT117970.3862701697401509No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC104330.341608602263202No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT74330.24337934828164293No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT70800.23182103939647947No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC59520.19488683989941324No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC58150.19040103730092206No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC57410.1879780490360436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56640.18545683151718356No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG50070.16394462489522213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46300.1516004819782062No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA45380.1485881181894384No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA38740.1268467099748533No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT33110.10841235331098072No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA32610.10677519907795474No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC31080.10176550712489521No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG120450.026.6743071
TACATGG125150.025.2543932
ACATGGG124450.024.5196763
CATGGGG63000.021.8795914
GAGTACT74000.021.03711712-13
GTATAGG7950.020.3266141
TAAGGTG12150.019.9521585
TACCTGG24850.019.5083242
GTACTTT80150.019.32936314-15
ATTCGCG3900.018.87480480-81
GTAAGGT11600.018.4389514
CTCGCGC3750.018.36050260-61
AGTACTT79350.018.06121812-13
GTACCTG27800.017.6094461
CATGGGA63650.017.5489524
GTGTAAG12450.017.1789421
CGCGGAA4050.017.00129782-83
AGAGTAC161000.016.57943310-11
GTATAGA8050.016.531621
AATTCGC4600.016.00556678-79
Produced by FastQC (version 0.11.5)