##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936580_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3054080 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40719431056161 31.0 31.0 34.0 30.0 34.0 2 31.464413178436715 31.0 31.0 34.0 28.0 34.0 3 31.42969404861693 31.0 31.0 34.0 28.0 34.0 4 35.0987937447611 37.0 35.0 37.0 32.0 37.0 5 35.138790077535624 37.0 35.0 37.0 32.0 37.0 6 35.205354149203686 37.0 35.0 37.0 32.0 37.0 7 35.075719365569995 37.0 35.0 37.0 32.0 37.0 8 35.05837830050293 37.0 35.0 37.0 32.0 37.0 9 36.552873205678964 39.0 37.0 39.0 32.0 39.0 10-11 36.42278493032272 39.0 37.0 39.0 32.0 39.0 12-13 36.33474974460394 39.0 35.0 39.0 32.0 39.0 14-15 37.32279884613369 39.5 37.0 41.0 32.0 41.0 16-17 37.24550093645222 39.0 37.0 41.0 32.0 41.0 18-19 37.11126574942372 39.0 36.0 41.0 31.0 41.0 20-21 37.07272320960813 39.0 36.0 41.0 31.0 41.0 22-23 37.07608919871123 39.0 36.0 41.0 31.0 41.0 24-25 37.181427794949705 39.0 36.5 41.0 31.0 41.0 26-27 37.10615275303856 39.0 36.0 41.0 31.0 41.0 28-29 37.01054081752934 39.0 36.0 41.0 30.5 41.0 30-31 36.84843160624476 39.0 36.0 41.0 30.0 41.0 32-33 36.701417448134954 39.0 36.0 41.0 30.0 41.0 34-35 36.49881470033529 39.0 35.0 40.0 30.0 41.0 36-37 36.28772052467519 39.0 35.0 40.0 30.0 41.0 38-39 36.09823432915968 38.0 35.0 40.0 29.5 41.0 40-41 35.9083337699078 38.0 35.0 40.0 28.5 41.0 42-43 35.65573069467729 38.0 35.0 40.0 27.5 41.0 44-45 35.5436150984912 38.0 35.0 40.0 27.0 41.0 46-47 35.44093786017393 38.0 35.0 40.0 27.0 41.0 48-49 35.22718527347024 38.0 34.0 40.0 26.0 41.0 50-51 34.24389030411777 37.5 33.0 39.5 25.0 40.5 52-53 34.3776353271689 37.5 33.5 39.5 25.5 40.5 54-55 34.838746856663874 38.0 34.0 40.0 26.0 41.0 56-57 34.6663409275461 38.0 34.0 40.0 25.5 41.0 58-59 34.42328262520955 38.0 34.0 40.0 25.0 41.0 60-61 34.086461716785415 37.0 33.0 40.0 24.0 41.0 62-63 33.76954418351844 37.0 33.0 40.0 23.0 41.0 64-65 33.40624001335918 36.0 32.5 39.0 22.5 41.0 66-67 33.0094431056161 36.0 32.0 39.0 22.0 40.5 68-69 32.55312958403185 35.0 31.5 39.0 21.5 40.0 70-71 32.10441605982817 35.0 31.0 38.0 20.0 40.0 72-73 31.676870448711234 35.0 31.0 37.0 19.0 39.5 74-75 31.276550876204944 35.0 31.0 37.0 18.0 39.0 76-77 30.822935875943003 34.5 30.0 36.0 16.0 39.0 78-79 30.34778034629086 34.0 30.0 36.0 12.0 37.5 80-81 29.864543987059932 34.0 29.0 35.0 7.5 37.0 82-83 29.460373172935874 34.0 29.0 35.0 7.0 37.0 84-85 29.089844241146267 34.0 29.0 35.0 3.5 36.0 86-87 28.781956923197818 34.0 29.0 35.0 2.0 36.0 88-89 28.4191771335394 34.0 28.5 35.0 2.0 35.5 90-91 28.028305414396478 34.0 27.0 35.0 2.0 35.0 92-93 27.579326016345348 33.0 26.5 35.0 2.0 35.0 94-95 27.06156256548617 33.0 25.5 35.0 2.0 35.0 96-97 26.432733425450543 33.0 25.0 35.0 2.0 35.0 98-99 25.680909635634954 32.5 23.5 35.0 2.0 35.0 100-101 23.079646079997907 29.5 9.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.43280234348085145 1101 2 0.33500988838857637 1101 3 0.4323266295233985 1101 4 0.5675826693808403 1101 5 0.43033583380800167 1101 6 0.42552186777996326 1101 7 0.4884833807907043 1101 8 0.3789193457606501 1101 9 0.4964208176600806 1101 10-11 0.6989169945965514 1101 12-13 0.7050278009515765 1101 14-15 0.8419798287905707 1101 16-17 0.9167289256258897 1101 18-19 1.0352254514091257 1101 20-21 0.9483372597413648 1101 22-23 0.856695853688997 1101 24-25 0.756321711174543 1101 26-27 0.6412526251712549 1101 28-29 0.63726096389194 1101 30-31 0.5501799078761636 1101 32-33 0.4686382628099963 1101 34-35 0.4944908078128236 1101 36-37 0.5121872557468166 1101 38-39 0.5185569021369432 1101 40-41 0.46899718241245125 1101 42-43 0.42261560367365547 1101 44-45 0.42724593133084454 1101 46-47 0.45554486648462245 1101 48-49 0.5373410912724239 1101 50-51 0.31106134090621396 1101 52-53 0.26422479026869183 1101 54-55 0.5044149219920584 1101 56-57 0.5978329101834419 1101 58-59 0.3655747180340896 1101 60-61 0.35794709371115374 1101 62-63 0.3969938126051389 1101 64-65 0.4020286657937504 1101 66-67 0.3034715135004902 1101 68-69 0.364106764934796 1101 70-71 0.26269389323021386 1101 72-73 0.3007827124162219 1101 74-75 0.16081280022672573 1101 76-77 0.2260699822914063 1101 78-79 0.28501486515490626 1101 80-81 0.28567101046182586 1101 82-83 0.3079615613498987 1101 84-85 0.2770984844254265 1101 86-87 0.25182228614300683 1101 88-89 0.18946408972074025 1101 90-91 0.17472122764846176 1101 92-93 0.32395986023527357 1101 94-95 0.4391402993183533 1101 96-97 0.3696112113557355 1101 98-99 0.27622788098276274 1101 100-101 0.6386263638553729 1102 1 0.33599662765800886 1102 2 0.36280463564392207 1102 3 0.43695313760715493 1102 4 0.4067834195574491 1102 5 0.3813400497804267 1102 6 0.31735665797437207 1102 7 0.4430627514367771 1102 8 0.2940812597165987 1102 9 0.5010224371767364 1102 10-11 0.5032949941088347 1102 12-13 0.3878477761635679 1102 14-15 0.5767893809723432 1102 16-17 0.46060219002544045 1102 18-19 0.5003183561563063 1102 20-21 0.6108534435949764 1102 22-23 0.4090048339365211 1102 24-25 0.5810096613370703 1102 26-27 0.34934228937671463 1102 28-29 0.35411278641382893 1102 30-31 0.2635177915434852 1102 32-33 0.29224431450279553 1102 34-35 0.03622038724631693 1102 36-37 0.18189007619968578 1102 38-39 0.017944115447043885 1102 40-41 0.15132868807020117 1102 42-43 0.12267983628873935 1102 44-45 0.104105848922039 1102 46-47 0.2015851960193089 1102 48-49 0.28997777098673083 1102 50-51 0.25898943054527734 1102 52-53 0.13678212824788716 1102 54-55 0.3489527614130381 1102 56-57 0.32367817871600124 1102 58-59 0.25110465596351617 1102 60-61 0.31568215868539085 1102 62-63 0.41517528796922676 1102 64-65 0.3091216560168206 1102 66-67 0.1785524013435591 1102 68-69 0.30468179139460716 1102 70-71 0.02112458108484816 1102 72-73 0.29854927776882434 1102 74-75 0.1876847850019594 1102 76-77 0.23464648195326987 1102 78-79 0.1460676515431274 1102 80-81 0.4356887341383917 1102 82-83 0.27385781373505225 1102 84-85 0.3358952101961563 1102 86-87 0.280976728500832 1102 88-89 0.37003780890228555 1102 90-91 0.6568526191707598 1102 92-93 0.7221515424258733 1102 94-95 0.7587263446256003 1102 96-97 0.7351747362807544 1102 98-99 0.6888369570135637 1102 100-101 1.2001654829006583 1103 1 0.3162593405988403 1103 2 0.4077579771262059 1103 3 0.3897433285136529 1103 4 0.46789923198641503 1103 5 0.3814296222574285 1103 6 0.3741507221069398 1103 7 0.4947612468501035 1103 8 0.3454095882084047 1103 9 0.3941890304368627 1103 10-11 0.28226138051098104 1103 12-13 0.3009863099792369 1103 14-15 0.5430419192715874 1103 16-17 0.6927950826225953 1103 18-19 0.7877550327039913 1103 20-21 0.6839993510565421 1103 22-23 0.5240250496032957 1103 24-25 0.4053528377453617 1103 26-27 0.4436014029226101 1103 28-29 0.3528063149260845 1103 30-31 0.38601466970346365 1103 32-33 0.3569925960474052 1103 34-35 0.28428781569143524 1103 36-37 0.2625811864200145 1103 38-39 0.10007490116097983 1103 40-41 0.3413363256497277 1103 42-43 0.34821087900804315 1103 44-45 0.3379955048680472 1103 46-47 0.5274677653472537 1103 48-49 0.6200483897296465 1103 50-51 0.5063748379845734 1103 52-53 0.5859831516505594 1103 54-55 0.6747245610940027 1103 56-57 0.5503658281065569 1103 58-59 0.6075459562985444 1103 60-61 0.33338930122440047 1103 62-63 0.41177549284770976 1103 64-65 0.47937608803988496 1103 66-67 0.44162231241441674 1103 68-69 0.5508797449191931 1103 70-71 0.4236361570490672 1103 72-73 0.48882736484382505 1103 74-75 0.46827683763032724 1103 76-77 0.3983109035344654 1103 78-79 0.4516033295968782 1103 80-81 0.516759001519663 1103 82-83 0.5333072151830578 1103 84-85 0.6099490493582991 1103 86-87 0.4608520363546731 1103 88-89 0.5606764953045911 1103 90-91 0.7340471036176481 1103 92-93 0.8308696002702227 1103 94-95 0.8264946867759662 1103 96-97 0.907997242561553 1103 98-99 0.9323384985509442 1103 100-101 1.3285887433718138 1104 1 0.12275004041914883 1104 2 0.1289160882750906 1104 3 0.31445477202457184 1104 4 0.38428017511690626 1104 5 0.26083567344484493 1104 6 0.18232743361520676 1104 7 0.27918971883624977 1104 8 0.2113299741375272 1104 9 0.2953019762222411 1104 10-11 0.3608535647986102 1104 12-13 0.33465797883133064 1104 14-15 0.3670014161391464 1104 16-17 0.4209890346728429 1104 18-19 0.34486574450364316 1104 20-21 0.4304937741863881 1104 22-23 0.1933049129740212 1104 24-25 0.2937251848873217 1104 26-27 -0.13283207124894858 1104 28-29 -0.1584036797317765 1104 30-31 -0.08026873890547392 1104 32-33 0.10186859365153822 1104 34-35 0.10390844282627398 1104 36-37 0.14280798453570043 1104 38-39 0.027672314270269283 1104 40-41 0.28777570855164214 1104 42-43 0.28807214525705405 1104 44-45 0.3710398770047476 1104 46-47 0.4582728905637694 1104 48-49 0.42639137129117444 1104 50-51 0.34475487457013543 1104 52-53 0.173769482248062 1104 54-55 0.3129406911184489 1104 56-57 0.5018426059868588 1104 58-59 0.397921201146616 1104 60-61 0.35326448941341226 1104 62-63 0.4130017567494164 1104 64-65 0.3273315054552626 1104 66-67 0.43299313785595217 1104 68-69 0.3011019293687127 1104 70-71 0.3278957041003707 1104 72-73 0.46365617354665645 1104 74-75 0.36431920241753346 1104 76-77 0.3648100212719889 1104 78-79 0.26017584509718006 1104 80-81 0.4780038010275369 1104 82-83 0.3987443802875532 1104 84-85 0.3141713633142338 1104 86-87 0.32196328236830496 1104 88-89 0.23385693012238917 1104 90-91 0.3767498216336129 1104 92-93 0.4318287571834425 1104 94-95 0.4242764846488214 1104 96-97 0.2943367436380733 1104 98-99 0.15133951841399096 1104 100-101 0.9182448228887523 1105 1 0.3704088969010293 1105 2 0.3104899791236946 1105 3 0.40778504628919876 1105 4 0.5260230795114538 1105 5 0.48819474119365225 1105 6 0.4356450892214312 1105 7 0.46828127102682515 1105 8 0.489967159029554 1105 9 0.3800921910262218 1105 10-11 0.5715175261013954 1105 12-13 0.5175969790894399 1105 14-15 0.740846378713357 1105 16-17 0.6562703733977884 1105 18-19 0.6950287355080675 1105 20-21 0.552409133579161 1105 22-23 0.541024711840258 1105 24-25 0.4885278749558637 1105 26-27 0.43164329846818106 1105 28-29 0.4432221674868657 1105 30-31 0.32014877617478277 1105 32-33 0.4269374226973852 1105 34-35 0.2750525823198018 1105 36-37 0.5453288483402261 1105 38-39 0.2923699444479766 1105 40-41 0.43927847605286985 1105 42-43 0.1723738650272395 1105 44-45 0.3607293815312147 1105 46-47 0.5055151879243667 1105 48-49 0.5012747442968006 1105 50-51 0.6024575139728583 1105 52-53 0.7289146924156427 1105 54-55 0.5498117896109065 1105 56-57 0.5136470263411397 1105 58-59 0.5366829474924657 1105 60-61 0.4873095342000866 1105 62-63 0.7458595004950581 1105 64-65 0.7988553996497245 1105 66-67 0.6885673346114274 1105 68-69 0.5508856436516893 1105 70-71 0.7869718175535851 1105 72-73 0.6063798918446643 1105 74-75 0.5898134332051477 1105 76-77 0.6336452808626447 1105 78-79 0.6900433491839024 1105 80-81 0.760691145086728 1105 82-83 0.5599979292852311 1105 84-85 0.680654246868869 1105 86-87 0.7989382936008234 1105 88-89 0.7695670164454853 1105 90-91 0.6861361437726643 1105 92-93 0.8220999627432981 1105 94-95 0.9223055362419288 1105 96-97 0.929880732090087 1105 98-99 1.2204759050802743 1105 100-101 1.521975213780376 1106 1 0.09733314594129183 1106 2 -0.024144066896877092 1106 3 0.03649094844155698 1106 4 0.17517000562538243 1106 5 0.2254748205197501 1106 6 0.07710567569388616 1106 7 0.32319568098053963 1106 8 0.2907641562302672 1106 9 0.2953358224561953 1106 10-11 0.38783226770678425 1106 12-13 0.38243470433837246 1106 14-15 0.24946646339370204 1106 16-17 0.08103518271106225 1106 18-19 0.22667729908720702 1106 20-21 0.14256142109205427 1106 22-23 -0.15212421821919264 1106 24-25 -0.19957277262263773 1106 26-27 -0.20847612602777588 1106 28-29 -0.12728987827898663 1106 30-31 -0.09720780266656703 1106 32-33 -0.009939999386553211 1106 34-35 -0.011473856895150902 1106 36-37 -0.22672210606806686 1106 38-39 0.06103396128008143 1106 40-41 0.04677343694863367 1106 42-43 0.04561318381572477 1106 44-45 -0.13476133495078102 1106 46-47 0.01700749703621085 1106 48-49 -0.12710699497715439 1106 50-51 -0.19582318005247856 1106 52-53 -0.13369279872775053 1106 54-55 0.12365237327627909 1106 56-57 0.3567592596357443 1106 58-59 0.14920084914578524 1106 60-61 0.26085792454581025 1106 62-63 0.16164221211144536 1106 64-65 -0.1336234465091195 1106 66-67 -0.08333246291470431 1106 68-69 0.011650726525623156 1106 70-71 -0.10053675964510589 1106 72-73 0.01825079980612898 1106 74-75 0.08969492815889524 1106 76-77 -0.024000869404673608 1106 78-79 0.09244871734066606 1106 80-81 0.18509702787541116 1106 82-83 0.23290597001912516 1106 84-85 0.4461181401113379 1106 86-87 0.489492539834 1106 88-89 0.5140171790223889 1106 90-91 0.6762071428343717 1106 92-93 0.70989542124655 1106 94-95 0.8017846858516684 1106 96-97 0.687268250576551 1106 98-99 0.7549436541634229 1106 100-101 1.3343209704062637 1107 1 0.19236551372711475 1107 2 0.2335867135873606 1107 3 0.25495978759011706 1107 4 0.23398519836914744 1107 5 0.2873576381629306 1107 6 0.24311858623444493 1107 7 0.3384195124486098 1107 8 0.28499921584550947 1107 9 0.3589833244677507 1107 10-11 0.6027671663974417 1107 12-13 0.4646456801265373 1107 14-15 0.2933595582660615 1107 16-17 0.3953044388005935 1107 18-19 0.6209836775525659 1107 20-21 0.42535666284467766 1107 22-23 0.4003545689946222 1107 24-25 0.33027828070191845 1107 26-27 0.3101216356575378 1107 28-29 0.49728495165298625 1107 30-31 0.3910291587846757 1107 32-33 0.45550007449245555 1107 34-35 0.35086018962667964 1107 36-37 0.31041627078804623 1107 38-39 0.41876199873161823 1107 40-41 0.42596799111438344 1107 42-43 0.4178998673063248 1107 44-45 0.38935678003714713 1107 46-47 0.47200804564275245 1107 48-49 0.4422437291834811 1107 50-51 0.336215441847898 1107 52-53 0.4400422060268383 1107 54-55 0.5045818956228487 1107 56-57 0.5648480058869367 1107 58-59 0.673403540975201 1107 60-61 0.5621229197912143 1107 62-63 0.6059929362720737 1107 64-65 0.5899314324317046 1107 66-67 0.5564250529948893 1107 68-69 0.4188836465753738 1107 70-71 0.5330352175328628 1107 72-73 0.5795301502559873 1107 74-75 0.5117950854261046 1107 76-77 0.6619813191696764 1107 78-79 0.6294728011677435 1107 80-81 0.5980948883099053 1107 82-83 0.5354761916561692 1107 84-85 0.5522392175745807 1107 86-87 0.7958275738476068 1107 88-89 0.841210690835716 1107 90-91 0.7543950040003438 1107 92-93 0.8360794970274021 1107 94-95 0.7567532324100767 1107 96-97 0.9400912883938268 1107 98-99 1.0266753307050074 1107 100-101 1.5327130960737314 1108 1 0.10217684544896244 1108 2 0.1785277326192123 1108 3 0.29016797811441464 1108 4 0.3580294634922083 1108 5 0.2839437505100548 1108 6 0.31262583325032267 1108 7 0.45421810059335144 1108 8 0.3970042614308227 1108 9 -0.05547639970870222 1108 10-11 0.16447253013790686 1108 12-13 0.1386528984135751 1108 14-15 0.5388969845899538 1108 16-17 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-5.276736108327739 2316 88-89 -6.8454303636866705 2316 90-91 -5.908971315593039 2316 92-93 -5.20696706925597 2316 94-95 -4.399708624479651 2316 96-97 -5.812033899996958 2316 98-99 -7.329512134939822 2316 100-101 -8.09602612341914 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6170.0 3 2321.0 4 2432.0 5 2673.0 6 2911.0 7 3467.0 8 4425.0 9 5589.0 10 7167.0 11 8984.0 12 10970.0 13 12944.0 14 14860.0 15 17353.0 16 20865.0 17 24367.0 18 21095.0 19 17928.0 20 19006.0 21 21148.0 22 23743.0 23 27432.0 24 31492.0 25 36757.0 26 43209.0 27 51775.0 28 62241.0 29 74698.0 30 91134.0 31 112535.0 32 141205.0 33 179106.0 34 236179.0 35 317956.0 36 432926.0 37 537489.0 38 379952.0 39 47564.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.418201239891474 17.75479376646526 13.533100908292594 23.29390408535067 2 15.524077130402627 19.937690928796233 39.11027467544356 25.427957265357577 3 20.87118896173922 23.1207981930818 29.299085635727305 26.708927209451673 4 12.6587999341073 15.466444666342879 37.12726166377758 34.74749373577224 5 13.726250535480583 37.47417575291518 34.07523322062586 14.72434049097838 6 32.85581776805962 35.13571495013042 16.9634516848751 15.045015596934853 7 29.482333596460787 32.171017772408184 20.074199976210394 18.27244865492063 8 26.911866504567318 32.937290680368456 19.43907989945123 20.711762915613004 9 26.023864897081072 17.15968537496879 20.550528274116697 36.265921453833435 10-11 25.58191230436519 25.39479154619768 26.99851292809013 22.024783221347 12-13 27.176623576560687 22.850923956035317 27.16612537894235 22.806327088461646 14-15 23.209650360805924 24.61602286542747 26.189454106099003 25.984872667667602 16-17 22.592637364329352 28.074892850859996 26.545091745478572 22.78737803933208 18-19 22.39735062891279 27.454433837627583 27.985497186214335 22.162718347245296 20-21 23.42981092610007 25.711980662453744 27.64021710528578 23.217991306160403 22-23 22.7631621604894 25.803476900294953 28.07305374436298 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23.445593734242287 26.492668775335126 26.992249661106854 23.06948782931573 50-51 22.397308234021367 26.783886797043078 27.962533085116853 22.856271883818707 52-53 23.331651974009166 27.023520858105556 26.60858364222376 23.036243525661522 54-55 22.497384641830998 26.241824447664186 27.310151191440944 23.950639719063872 56-57 23.320006483332435 26.336426013880228 26.883268909310292 23.46029859347705 58-59 24.13484574302013 26.046254091179748 26.614447351217436 23.204452814582684 60-61 23.098467811333062 26.248046780347433 28.076656585673813 22.57682882264569 62-63 22.762755899336888 26.535551554620756 27.758059612473247 22.94363293356911 64-65 22.441620049858116 27.35811417334888 27.10273335567513 23.09753242111788 66-67 22.73187909903833 26.709407446456694 26.519922595094414 24.038790859410557 68-69 22.510593229010702 26.5558388129755 27.253540531288028 23.680027426725765 70-71 23.32564395161092 26.456019083200854 26.78196919117418 23.436367774014037 72-73 23.026561413115385 25.938855433129454 27.75589725988815 23.278685893867006 74-75 22.390893704871992 27.601721414124476 26.93641077098813 23.070974110015406 76-77 22.86699446403885 26.402584867761504 26.846758713251756 23.883661954947893 78-79 22.980853041703654 26.608637443111693 27.065790742735746 23.344718772448907 80-81 23.468236088526893 26.536225721409174 26.837025392365017 23.15851279769892 82-83 22.92945817330818 26.306055737051103 27.582322155558035 23.18216393408268 84-85 22.735213200898237 26.806875238623512 27.12298794947174 23.334923611006513 86-87 22.912662399960183 26.956068596485828 26.88951727452936 23.241751729024628 88-89 22.547045524832463 26.993292319852756 26.194824456092782 24.264837699221996 90-91 22.73956701196341 26.241619309111808 26.8634808989831 24.15533277994168 92-93 23.3521914102723 26.59489867542653 27.068902085303915 22.984007828997253 94-95 22.87507804071429 26.295836076595087 27.15487747710192 23.674208405588708 96-97 22.143058885940775 26.279835316828446 27.85550844578415 23.721597351446626 98-99 22.515692085189137 26.86151254818241 26.499044252900195 24.123751113728257 100-101 22.253428598657216 25.98052010360546 27.142953987005765 24.623097310731563 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 158.0 1 128.5 2 152.5 3 208.5 4 187.0 5 202.0 6 345.0 7 666.0 8 1119.5 9 1328.0 10 1244.5 11 1162.0 12 1220.0 13 1371.0 14 1515.5 15 1674.5 16 1822.5 17 1990.0 18 2204.0 19 2476.5 20 2757.5 21 3194.5 22 3949.5 23 4829.0 24 6033.0 25 7732.5 26 10119.5 27 13429.5 28 17072.0 29 21222.0 30 26838.5 31 33760.5 32 42357.0 33 47732.0 34 51594.0 35 57164.5 36 63648.5 37 70662.5 38 74368.5 39 76053.5 40 79013.0 41 84800.5 42 90217.5 43 95149.5 44 100429.0 45 106873.0 46 112141.0 47 131989.0 48 174744.5 49 173340.0 50 135012.0 51 117188.5 52 109193.0 53 107167.5 54 105991.0 55 98777.0 56 89938.5 57 81284.0 58 74427.0 59 66667.0 60 56284.0 61 47149.5 62 39378.0 63 30757.5 64 22518.0 65 17275.5 66 13174.0 67 10185.5 68 7438.0 69 5010.5 70 3704.5 71 2770.0 72 2099.0 73 1747.5 74 1365.0 75 1050.5 76 769.5 77 487.0 78 311.0 79 209.0 80 136.0 81 80.0 82 61.5 83 43.0 84 29.0 85 20.0 86 16.0 87 14.5 88 11.0 89 7.5 90 4.0 91 3.0 92 3.0 93 3.0 94 2.5 95 2.5 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07203478625314334 2 0.029894436295054484 3 0.015814909891031015 4 0.02066088642078793 5 0.024491827326068735 6 0.029992665549036044 7 0.07619315800502934 8 0.05340397108130763 9 0.0676472129086337 10-11 0.05805348910310142 12-13 0.03847312447611064 14-15 0.0413054012992456 16-17 0.051046468985750215 18-19 0.046855354149203686 20-21 0.01763215108968986 22-23 0.012540601424979045 24-25 0.021364862740989102 26-27 0.023427677074601843 28-29 0.005877383696563285 30-31 0.0011787510477787092 32-33 0.009151692162615256 34-35 0.010543273260687344 36-37 0.0038309409052808044 38-39 0.008840632858340318 40-41 0.004109257124895222 42-43 0.0018663558256496228 44-45 3.4380238893545685E-4 46-47 3.27430846605197E-4 48-49 6.54861693210394E-4 50-51 8.349486588432524E-4 52-53 1.1460079631181894E-4 54-55 1.637154233025985E-4 56-57 0.0031105930427493714 58-59 0.003323423093042749 60-61 0.0048459765297569155 62-63 0.0023411305532271584 64-65 4.4203164291701597E-4 66-67 3.27430846605197E-4 68-69 0.0040765140402347026 70-71 0.0027831621961441743 72-73 0.02056265716680637 74-75 0.017812238055322716 76-77 0.02524491827326069 78-79 0.020251597862531435 80-81 0.012655202221290865 82-83 0.004354830259849119 84-85 0.0034707669740150883 86-87 0.0015061818943839062 88-89 0.0014243241827326069 90-91 0.006057470662196144 92-93 0.009970269279128249 94-95 0.012622459136630345 96-97 0.011050791072925397 98-99 0.023231218566638723 100-101 0.018761787510477787 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3054080.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.476271607175846 #Duplication Level Percentage of deduplicated Percentage of total 1 72.69252078472795 33.784773398012916 2 14.191739767368873 13.191583040131885 3 5.286568816175989 7.371000245118642 4 2.5097780198526625 4.665804996975693 5 1.3864252973405857 3.2217939341130304 6 0.8708238975983486 2.4283588792080204 7 0.5830498532721932 1.8968588338841734 8 0.3904716169498544 1.4518131939403651 9 0.30175562720910276 1.2622028840247568 >10 1.586214152353908 13.300658723710427 >50 0.12234794786637163 3.930907435653281 >100 0.06988017977072447 5.938178334898359 >500 0.004742946616844198 1.479115328463897 >1k 0.0029024001684011787 2.62580230800576 >5k 4.955317360684939E-4 1.4045953155416855 >10k+ 2.831609920391394E-4 2.046553148317132 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24618 0.8060692581726739 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15356 0.5028028080469404 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11797 0.3862701697401509 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10433 0.341608602263202 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7433 0.24337934828164293 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7080 0.23182103939647947 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5952 0.19488683989941324 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5815 0.19040103730092206 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5741 0.1879780490360436 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5664 0.18545683151718356 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5007 0.16394462489522213 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4630 0.1516004819782062 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4538 0.1485881181894384 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3874 0.1268467099748533 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3311 0.10841235331098072 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3261 0.10677519907795474 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3108 0.10176550712489521 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.27430846605197E-5 0.0 0.0 0.0 0.0 7 9.822925398155909E-5 0.0 0.0 0.0 0.0 8 9.822925398155909E-5 0.0 0.0 0.0 0.0 9 9.822925398155909E-5 0.0 0.0 0.0 3.27430846605197E-5 10-11 9.822925398155909E-5 0.0 0.0 0.0 6.54861693210394E-5 12-13 1.309723386420788E-4 0.0 0.0 0.0 1.1460079631181894E-4 14-15 1.309723386420788E-4 0.0 0.0 0.0 1.4734388097233865E-4 16-17 1.4734388097233865E-4 0.0 0.0 1.637154233025985E-5 1.637154233025985E-4 18-19 2.4557313495389774E-4 0.0 0.0 6.54861693210394E-5 1.637154233025985E-4 20-21 2.946877619446773E-4 0.0 0.0 9.822925398155909E-5 1.637154233025985E-4 22-23 2.946877619446773E-4 0.0 0.0 1.9645850796311818E-4 1.8008696563285833E-4 24-25 4.911462699077955E-4 0.0 0.0 3.765454735959765E-4 2.2920159262363788E-4 26-27 4.911462699077955E-4 0.0 0.0 4.911462699077955E-4 2.4557313495389774E-4 28-29 5.730039815590947E-4 0.0 0.0 9.986640821458507E-4 2.619446772841576E-4 30-31 5.893755238893546E-4 0.0 0.0 0.002603075230511316 2.619446772841576E-4 32-33 5.893755238893546E-4 0.0 0.0 0.00433845871751886 2.619446772841576E-4 34-35 6.712332355406539E-4 0.0 0.0 0.006810561609388097 2.946877619446773E-4 36-37 7.203478625314334E-4 0.0 0.0 0.010133984702430847 3.1105930427493715E-4 38-39 7.858340318524727E-4 0.0 0.0 0.015667566010058677 3.27430846605197E-4 40-41 7.858340318524727E-4 0.0 0.0 0.02719313181056161 3.27430846605197E-4 42-43 9.004348281642917E-4 0.0 0.0 0.03801472129086337 3.27430846605197E-4 44-45 0.0010477787091366304 0.0 0.0 0.05191416072925398 3.27430846605197E-4 46-47 0.001195122590108969 0.0 0.0 0.07753562447611065 3.27430846605197E-4 48-49 0.0012442372170997486 0.0 0.0 0.09659209974853311 3.601739312657167E-4 50-51 0.0012442372170997486 0.0 0.0 0.12227904966471081 3.601739312657167E-4 52-53 0.0012606087594300083 0.0 0.0 0.15128942267393125 4.2566010058675606E-4 54-55 0.0012769803017602683 0.0 0.0 0.18624266554903607 4.911462699077955E-4 56-57 0.0012769803017602683 0.0 0.0 0.2524655542749371 4.911462699077955E-4 58-59 0.0012769803017602683 0.0 0.0 0.3149393598072087 4.911462699077955E-4 60-61 0.0012769803017602683 0.0 0.0 0.37148666701592625 4.911462699077955E-4 62-63 0.0012769803017602683 0.0 0.0 0.4342060456831517 5.238893545683152E-4 64-65 0.001309723386420788 0.0 0.0 0.5020169740150879 5.238893545683152E-4 66-67 0.0014406957250628668 0.0 0.0 0.6002789710813077 5.40260896898575E-4 68-69 0.0014406957250628668 0.0 0.0 0.7183832774518022 5.566324392288349E-4 70-71 0.001506181894383906 0.0 0.0 0.8120121280385583 5.566324392288349E-4 72-73 0.0015552965213746855 0.0 0.0 0.9117475639145013 6.712332355406539E-4 74-75 0.0016207826906957249 0.0 0.0 1.0465344719195304 6.876047778709137E-4 76-77 0.0016371542330259848 1.637154233025985E-5 0.0 1.2007544006705784 6.876047778709137E-4 78-79 0.0016371542330259848 3.27430846605197E-5 0.0 1.377714401718357 7.367194048616933E-4 80-81 0.001735383487007544 3.27430846605197E-5 0.0 1.5597659524308467 7.53090947191953E-4 82-83 0.0018008696563285834 3.27430846605197E-5 0.0 1.7594823973176865 7.53090947191953E-4 84-85 0.001817241198658843 3.27430846605197E-5 0.0 2.016008094090528 7.53090947191953E-4 86-87 0.001849984283319363 3.27430846605197E-5 0.0 2.2768231349538977 8.185771165129924E-4 88-89 0.0018663558256496228 3.27430846605197E-5 0.0 2.5917788663034367 8.840632858340318E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12045 0.0 26.674307 1 TACATGG 12515 0.0 25.254393 2 ACATGGG 12445 0.0 24.519676 3 CATGGGG 6300 0.0 21.879591 4 GAGTACT 7400 0.0 21.037117 12-13 GTATAGG 795 0.0 20.326614 1 TAAGGTG 1215 0.0 19.952158 5 TACCTGG 2485 0.0 19.508324 2 GTACTTT 8015 0.0 19.329363 14-15 ATTCGCG 390 0.0 18.874804 80-81 GTAAGGT 1160 0.0 18.438951 4 CTCGCGC 375 0.0 18.360502 60-61 AGTACTT 7935 0.0 18.061218 12-13 GTACCTG 2780 0.0 17.609446 1 CATGGGA 6365 0.0 17.548952 4 GTGTAAG 1245 0.0 17.178942 1 CGCGGAA 405 0.0 17.001297 82-83 AGAGTAC 16100 0.0 16.579433 10-11 GTATAGA 805 0.0 16.53162 1 AATTCGC 460 0.0 16.005566 78-79 >>END_MODULE