FastQCFastQC Report
Thu 26 May 2016
SRR936580_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936580_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3054080
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT296990.9724368713327745No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT122210.4001532376362112No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT107070.35058020746018437No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC92690.3034956517183571No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT82100.26882072506286675No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC75940.24865098491198662No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC75640.24766869237217098No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT67550.22117953688181058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60710.1987832669740151No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG59060.19338065800502932No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA57450.18810902137468566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55600.18205155071248952No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC51460.16849591366303437No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA46410.15196065590947191No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC36070.11810430637049454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33130.10847783948030175No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT32350.10592387887678123No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA30710.10055401299245599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGGT10950.026.8896884
TAAGGTG12500.025.4549945
GTACATG122650.024.791251
TACATGG129100.022.8485622
ACATGGG127650.022.1745593
CATGGGG63700.020.2785874
GAGTACT86600.020.20820812-13
GGTAAGG13900.019.4755443
GTACTTT91700.019.0066214-15
AGTACTT91950.018.64505612-13
AGGTAAG14700.017.7721062
GTACTAG6050.017.2764021
AGAGTAC177500.016.60139310-11
TACCTGG21500.016.5697652
TACTTTT102850.016.3227514-15
GTATAGA6450.016.2049961
ACTTTTT109100.016.19296516-17
GTACCTG24500.015.90134051
AGTACAT97350.015.7601412
TAGACTG10950.015.6133685
Produced by FastQC (version 0.11.5)