##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936580_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3054080 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.686756731978207 31.0 31.0 34.0 30.0 34.0 2 31.844380304379715 31.0 31.0 34.0 30.0 34.0 3 31.935647396269907 31.0 31.0 34.0 30.0 34.0 4 35.66068996227997 37.0 35.0 37.0 33.0 37.0 5 35.47518303384325 37.0 35.0 37.0 33.0 37.0 6 35.56787837908634 37.0 35.0 37.0 33.0 37.0 7 35.50057136682732 37.0 35.0 37.0 33.0 37.0 8 35.46133401875524 37.0 35.0 37.0 33.0 37.0 9 37.04878752357502 39.0 37.0 39.0 33.0 39.0 10-11 36.919947414606035 39.0 37.0 39.0 33.0 39.0 12-13 36.81911623140193 39.0 37.0 39.0 32.0 39.0 14-15 37.87001273705994 40.0 37.0 41.0 33.0 41.0 16-17 37.76737986562238 39.0 37.0 41.0 32.5 41.0 18-19 37.90939873873638 40.0 37.0 41.0 33.0 41.0 20-21 37.917326166963534 40.0 37.0 41.0 33.0 41.0 22-23 37.827071982397314 39.5 37.0 41.0 32.0 41.0 24-25 37.67253297228625 39.0 37.0 41.0 32.0 41.0 26-27 37.448416871856665 39.0 36.0 41.0 32.0 41.0 28-29 37.379861529494974 39.0 36.0 41.0 31.5 41.0 30-31 37.40707643545683 39.0 36.0 41.0 32.0 41.0 32-33 37.2819652726844 39.0 36.0 41.0 31.0 41.0 34-35 37.09704591890193 39.0 36.0 41.0 31.0 41.0 36-37 36.89403568341366 39.0 35.5 40.5 30.5 41.0 38-39 36.730384272841576 39.0 35.0 40.0 30.0 41.0 40-41 36.57815528735331 39.0 35.0 40.0 30.0 41.0 42-43 36.31040526115885 38.5 35.0 40.0 30.0 41.0 44-45 36.06826474748533 38.0 35.0 40.0 29.5 41.0 46-47 35.74654494970662 38.0 35.0 40.0 28.5 41.0 48-49 35.39476225246228 38.0 34.0 40.0 27.0 41.0 50-51 35.10184638254401 38.0 34.0 40.0 27.0 41.0 52-53 34.74216261525566 38.0 33.5 40.0 26.0 41.0 54-55 34.5503041832565 38.0 33.0 40.0 26.0 41.0 56-57 34.20160375628667 37.0 33.0 40.0 25.0 41.0 58-59 33.798041636106454 37.0 32.5 39.5 24.0 41.0 60-61 33.30774652268441 36.0 32.0 39.0 23.0 40.0 62-63 32.98195725062867 36.0 31.0 39.0 22.0 40.0 64-65 32.541278879400664 35.0 31.0 38.5 22.0 40.0 66-67 32.06433967021165 35.0 31.0 38.0 20.5 40.0 68-69 31.670785801288766 35.0 30.0 37.5 20.0 39.5 70-71 31.244727217361692 34.0 30.0 37.0 18.5 39.0 72-73 30.7442635752829 34.0 29.5 36.5 17.5 39.0 74-75 30.228552624685666 34.0 29.0 36.0 14.0 38.5 76-77 28.777883847181478 32.0 27.0 34.5 12.0 36.5 78-79 29.576634043639984 33.5 29.0 35.0 11.0 37.0 80-81 29.32942277216052 34.0 29.0 35.0 7.0 37.0 82-83 29.000828072611064 33.0 29.0 35.0 7.0 36.0 84-85 28.59999492482188 33.0 28.0 35.0 2.0 36.0 86-87 28.217348268545685 33.0 27.0 35.0 2.0 35.0 88-89 27.6933495520746 33.0 26.5 35.0 2.0 35.0 90-91 27.14534999083194 32.0 25.5 34.0 2.0 35.0 92-93 26.467505435352052 32.0 25.0 34.0 2.0 35.0 94-95 25.835482862269487 32.0 24.0 34.0 2.0 35.0 96-97 24.985141679327327 31.0 19.5 34.0 2.0 35.0 98-99 23.988134724696145 31.0 9.5 34.0 2.0 35.0 100-101 20.707195129138725 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.28126456108957854 1101 2 0.3413708313712611 1101 3 0.2987138789298598 1101 4 0.18322617462776947 1101 5 0.20474019856414571 1101 6 0.1995940157305185 1101 7 0.25558537256448943 1101 8 0.21116849662009685 1101 9 0.23702130256057785 1101 10-11 0.23916432542500132 1101 12-13 0.2617785688152807 1101 14-15 0.40185422021312434 1101 16-17 0.3623006627291758 1101 18-19 0.30592960716519 1101 20-21 0.3752236557890356 1101 22-23 0.25651860896614664 1101 24-25 0.3091538472261348 1101 26-27 0.36914847255843597 1101 28-29 0.3653703180894965 1101 30-31 0.33545343258552407 1101 32-33 0.2536181801138895 1101 34-35 0.3840223285678448 1101 36-37 0.41337811203614194 1101 38-39 0.22488971984834194 1101 40-41 0.292407117128036 1101 42-43 0.4641258476309318 1101 44-45 0.48146370152520035 1101 46-47 0.46533120400329864 1101 48-49 0.4672136944707077 1101 50-51 0.4962483657683876 1101 52-53 0.553574803802583 1101 54-55 0.49582111884439684 1101 56-57 0.41629224868417936 1101 58-59 0.30849490227498677 1101 60-61 0.3712106314653667 1101 62-63 0.430198432369977 1101 64-65 0.4519478472159193 1101 66-67 0.327834252377194 1101 68-69 0.25874255362662524 1101 70-71 0.16423234023431377 1101 72-73 0.3284987275214384 1101 74-75 0.2530014561986782 1101 76-77 0.09661721854370597 1101 78-79 0.3548144449644681 1101 80-81 0.3678888437785517 1101 82-83 0.3088915531470704 1101 84-85 0.31200157989695754 1101 86-87 0.3887590819415401 1101 88-89 0.36569276189569067 1101 90-91 0.4721595533775691 1101 92-93 0.33707473346484207 1101 94-95 0.1842274178305665 1101 96-97 0.615645036488683 1101 98-99 0.6373133462518403 1101 100-101 1.3465042379146759 1102 1 0.1957849030936849 1102 2 0.15902763562896283 1102 3 0.20162458428190888 1102 4 0.1987913336506253 1102 5 0.19311472373302507 1102 6 0.10285014422591843 1102 7 0.06340189103104876 1102 8 0.1554754254463333 1102 9 0.17297019803613978 1102 10-11 0.22817325765519314 1102 12-13 0.2520657426556312 1102 14-15 0.32203294023685913 1102 16-17 0.44999188360138476 1102 18-19 0.30430732510985337 1102 20-21 0.2016477720563543 1102 22-23 0.2103187663423398 1102 24-25 0.15516853399471842 1102 26-27 0.16800284427294798 1102 28-29 0.20251650448224012 1102 30-31 0.26741915172684827 1102 32-33 0.2042972106162111 1102 34-35 0.09800090428817043 1102 36-37 0.1850793084471647 1102 38-39 0.13365275806359733 1102 40-41 0.1961269523104079 1102 42-43 0.2823361082569633 1102 44-45 0.2053935559899287 1102 46-47 0.1792058259610556 1102 48-49 0.22306213063907165 1102 50-51 0.10539076608213094 1102 52-53 0.3282706730546394 1102 54-55 0.18756867335412863 1102 56-57 0.08737713880995557 1102 58-59 0.3536833640358452 1102 60-61 0.37230610551714705 1102 62-63 0.2928195755848222 1102 64-65 0.13396196079325762 1102 66-67 0.07819777629661218 1102 68-69 0.20971182504524677 1102 70-71 0.16823650844080618 1102 72-73 0.3689146179916314 1102 74-75 0.20458615400370306 1102 76-77 0.14607897185526753 1102 78-79 0.2697580183090622 1102 80-81 0.2724282418774706 1102 82-83 0.23389890658734913 1102 84-85 0.4282471521203135 1102 86-87 0.46217042459174706 1102 88-89 0.38387675125645515 1102 90-91 0.4543707642943495 1102 92-93 0.571768767499325 1102 94-95 0.2552416646497342 1102 96-97 0.48428833304224383 1102 98-99 0.4561598994366953 1102 100-101 1.1026733343591353 1103 1 0.18294997543624447 1103 2 0.21182623942003787 1103 3 0.18333035436602074 1103 4 0.18751707856206679 1103 5 0.2987797292673875 1103 6 0.1815361836213114 1103 7 0.11621649579868176 1103 8 0.12408249919764103 1103 9 0.23678440195456574 1103 10-11 0.2433736905063455 1103 12-13 0.28680369779681314 1103 14-15 0.3865507747273753 1103 16-17 0.29589967508721315 1103 18-19 0.23599742413444602 1103 20-21 0.2531301718069372 1103 22-23 0.19109796441298244 1103 24-25 0.24178940817591155 1103 26-27 0.16731249286181082 1103 28-29 0.11934153132924052 1103 30-31 0.21451588175407466 1103 32-33 0.20282281135390434 1103 34-35 0.21907402545185306 1103 36-37 0.16238588220918615 1103 38-39 0.23127686048304952 1103 40-41 0.21323768731609505 1103 42-43 0.3664910999842945 1103 44-45 0.4376812997367665 1103 46-47 0.5305543618716939 1103 48-49 0.25441126040039563 1103 50-51 0.3675681690741541 1103 52-53 0.4003945799982418 1103 54-55 0.3210301794584183 1103 56-57 0.266223847804973 1103 58-59 0.34392697477992584 1103 60-61 0.3140973571069381 1103 62-63 0.4192221766507842 1103 64-65 0.5066347672160845 1103 66-67 0.3638562824652425 1103 68-69 0.3106542330767468 1103 70-71 0.3924343563919379 1103 72-73 0.43745298651886344 1103 74-75 0.4432724738285856 1103 76-77 0.39066363104271673 1103 78-79 0.2821656209173895 1103 80-81 0.4294775793215706 1103 82-83 0.34956567717788545 1103 84-85 0.70200629288437 1103 86-87 0.7955822781811612 1103 88-89 0.49830913723300085 1103 90-91 0.6933196156056916 1103 92-93 0.6852562710665531 1103 94-95 0.4633670265274894 1103 96-97 0.6071091072585659 1103 98-99 0.6019302804743845 1103 100-101 1.022036742930048 1104 1 0.1316889066845235 1104 2 0.1655535960321508 1104 3 0.13450076862467597 1104 4 0.15301252155496314 1104 5 0.11565846269461133 1104 6 0.06362949299595755 1104 7 0.026454787609019093 1104 8 0.12173519535939192 1104 9 0.14682195881597693 1104 10-11 0.2138947919584382 1104 12-13 0.08973418522659671 1104 14-15 0.18230903480863958 1104 16-17 0.23573342122225682 1104 18-19 0.16914661552875287 1104 20-21 0.15094960748271546 1104 22-23 0.07336044154700971 1104 24-25 0.11481281646107533 1104 26-27 0.22794254158860383 1104 28-29 0.18169158717888223 1104 30-31 0.0625332194258732 1104 32-33 0.14328709186480637 1104 34-35 0.1702816728058778 1104 36-37 0.14019576678659007 1104 38-39 0.06562196271217857 1104 40-41 0.18663342655491988 1104 42-43 0.21798689911984326 1104 44-45 0.16563647432761286 1104 46-47 0.12399081469088458 1104 48-49 0.24890885643749527 1104 50-51 0.2129286016255847 1104 52-53 0.39543205591814257 1104 54-55 0.2797212742733066 1104 56-57 0.1336262728126698 1104 58-59 0.23167971465009884 1104 60-61 0.2877403726638974 1104 62-63 0.11064399524467206 1104 64-65 0.22187605386235276 1104 66-67 0.20151310664408584 1104 68-69 0.14514745827221986 1104 70-71 0.13788220577597343 1104 72-73 0.34013862587586274 1104 74-75 0.3076229991389958 1104 76-77 0.1978026723714521 1104 78-79 0.1620067327299921 1104 80-81 0.4038502826061716 1104 82-83 0.40206069927491583 1104 84-85 0.6062605787374125 1104 86-87 0.6422920057320347 1104 88-89 0.46407371075217796 1104 90-91 0.4860352842635187 1104 92-93 0.5760892401630855 1104 94-95 0.6146624954216655 1104 96-97 0.8303959801642051 1104 98-99 0.7892592915309926 1104 100-101 1.271549879737389 1105 1 -0.03632805202760636 1105 2 0.12383363465289321 1105 3 0.07829946278472022 1105 4 0.13024588069918508 1105 5 0.17505745129002293 1105 6 0.16327435423851 1105 7 0.033213895508112046 1105 8 0.18254087757951254 1105 9 0.16005482086153933 1105 10-11 0.12713625763473146 1105 12-13 0.15337978255755758 1105 14-15 0.1955531293721009 1105 16-17 0.17122196405399848 1105 18-19 0.20107636210402546 1105 20-21 0.19051722724047693 1105 22-23 0.1932960099124088 1105 24-25 0.15358388340688833 1105 26-27 0.10149354677945865 1105 28-29 0.1602116670970517 1105 30-31 0.07449871886664283 1105 32-33 0.17562957372548027 1105 34-35 0.3529228918447487 1105 36-37 0.3016471992911036 1105 38-39 0.21887174523151032 1105 40-41 0.36955450258233213 1105 42-43 0.3797428045563791 1105 44-45 0.4783289805652373 1105 46-47 0.35438314025227413 1105 48-49 0.31586756856956555 1105 50-51 0.33670664707187825 1105 52-53 0.453187751043707 1105 54-55 0.22973250679120127 1105 56-57 0.13497656485812826 1105 58-59 0.10270364230311912 1105 60-61 0.40891964364958255 1105 62-63 0.35842951629010145 1105 64-65 0.40294854423106585 1105 66-67 0.23760748847321622 1105 68-69 0.3897176113212524 1105 70-71 0.3999841683597367 1105 72-73 0.5552217465083267 1105 74-75 0.3820589833465533 1105 76-77 0.273885534894994 1105 78-79 0.35819890916177854 1105 80-81 0.4204005612682131 1105 82-83 0.7310919782524259 1105 84-85 0.6492390601009888 1105 86-87 0.7718808477789345 1105 88-89 0.7918464697453622 1105 90-91 1.0491794811145887 1105 92-93 0.8923613347647894 1105 94-95 1.0295250285217463 1105 96-97 1.1397170581035425 1105 98-99 0.9202952164690963 1105 100-101 1.1810298858555264 1106 1 0.04334372716930801 1106 2 0.10544658986172095 1106 3 0.07496296498490196 1106 4 0.07311978808103703 1106 5 0.1108217056845362 1106 6 -0.07045106104085619 1106 7 -0.03536405693262168 1106 8 -0.044065137895834994 1106 9 0.013238358618103518 1106 10-11 -0.06078301792987162 1106 12-13 -0.0526302345708487 1106 14-15 0.057716003227504586 1106 16-17 0.07552406273856604 1106 18-19 0.13491293982773556 1106 20-21 0.03464483516373207 1106 22-23 -0.13112418445211205 1106 24-25 -0.1503940686439691 1106 26-27 -0.1142044812445846 1106 28-29 -0.2543966343316981 1106 30-31 -0.09483126546047771 1106 32-33 -0.04270935428062472 1106 34-35 -0.04519988750551107 1106 36-37 -0.3857480224294747 1106 38-39 -0.09383529339967112 1106 40-41 0.0513898963927204 1106 42-43 -0.005797565458770748 1106 44-45 -0.024739804669508203 1106 46-47 0.04564784819866219 1106 48-49 -0.035107096854702036 1106 50-51 0.04816513472980688 1106 52-53 -0.16867677925069557 1106 54-55 -0.1696022892144171 1106 56-57 -0.2618515123418561 1106 58-59 -0.010892176052607283 1106 60-61 -0.049995574640121276 1106 62-63 -0.03811882727088545 1106 64-65 -0.09708743776721462 1106 66-67 -0.06804171774602708 1106 68-69 -0.19820468575652228 1106 70-71 -0.0027726537621575176 1106 72-73 0.18750984746455046 1106 74-75 0.007959020644374704 1106 76-77 -0.16719869449568847 1106 78-79 -0.1875721420129075 1106 80-81 -0.0022910189116984725 1106 82-83 0.045798495104019565 1106 84-85 0.26422355616457693 1106 86-87 0.48313790122528033 1106 88-89 0.21656075805879738 1106 90-91 0.32537697501308216 1106 92-93 0.33314292549725266 1106 94-95 0.39322562515688375 1106 96-97 0.6138554232634803 1106 98-99 0.616379562821578 1106 100-101 1.0560115293507621 1107 1 0.014788756794061442 1107 2 0.07481605807911862 1107 3 0.051898631724718314 1107 4 0.1137441023228618 1107 5 0.034236232785694654 1107 6 0.009581489613815108 1107 7 0.05906941538103183 1107 8 0.04768930581713704 1107 9 -0.04587856772231902 1107 10-11 0.0654513467837603 1107 12-13 -0.028710989453450964 1107 14-15 0.03493740253991717 1107 16-17 0.13596450235527868 1107 18-19 -0.05617814714468494 1107 20-21 0.04590041266354916 1107 22-23 0.0060183069530594935 1107 24-25 0.050055331546211335 1107 26-27 0.07269205156208614 1107 28-29 0.10383886416877175 1107 30-31 -0.02316953625266649 1107 32-33 0.016338996242375003 1107 34-35 0.0460493810191025 1107 36-37 -0.018396511932806447 1107 38-39 -0.0404855933045809 1107 40-41 -0.01398343035733518 1107 42-43 0.03128823660399149 1107 44-45 0.05704371519005491 1107 46-47 -0.07497244292854788 1107 48-49 -0.07319181999126556 1107 50-51 -0.1280107119529461 1107 52-53 0.09154787512718343 1107 54-55 -0.0601255684185773 1107 56-57 -0.006134575029300038 1107 58-59 -0.12313935987236846 1107 60-61 -0.14080367207264288 1107 62-63 -0.00933755669814218 1107 64-65 -0.058358101946865304 1107 66-67 -0.042387504447809476 1107 68-69 0.015756436326718415 1107 70-71 0.049495834278218354 1107 72-73 0.2025622393890636 1107 74-75 0.3236660830392246 1107 76-77 0.31852839203785166 1107 78-79 0.30900363883706916 1107 80-81 0.5579391215315752 1107 82-83 0.4101418130763683 1107 84-85 0.5572800260643618 1107 86-87 0.5915425534087326 1107 88-89 0.4660395818463776 1107 90-91 0.47418192710144424 1107 92-93 0.6935774218764514 1107 94-95 0.7108345235549542 1107 96-97 0.805677985621756 1107 98-99 0.6943594106746964 1107 100-101 1.0914103663048955 1108 1 -0.005756202497849472 1108 2 0.126518609066121 1108 3 -0.0019009572524950613 1108 4 0.09323321676652085 1108 5 0.04200855208465981 1108 6 0.13995344786480501 1108 7 0.07913116233477524 1108 8 0.1206511953460776 1108 9 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2316 56-57 -1.1822776424072643 2316 58-59 -2.0642906724914525 2316 60-61 -1.2937380008718904 2316 62-63 -1.2675899468648595 2316 64-65 -1.0493124978730677 2316 66-67 -1.1481255726783708 2316 68-69 -1.692764890622243 2316 70-71 -1.4716514616322058 2316 72-73 -1.035850734394561 2316 74-75 -1.1934707265343647 2316 76-77 -1.1693523082727921 2316 78-79 -1.4981009443943236 2316 80-81 -1.2336645937518291 2316 82-83 -1.5928899811452872 2316 84-85 -1.425926186515639 2316 86-87 -1.6355240789424776 2316 88-89 -1.6896552743305655 2316 90-91 -1.7081606931165005 2316 92-93 -1.7147550376837408 2316 94-95 -1.7300328789875685 2316 96-97 -2.053942084576647 2316 98-99 -2.634381715232305 2316 100-101 -3.41099926172193 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 22.0 4 42.0 5 99.0 6 231.0 7 584.0 8 1282.0 9 2368.0 10 3789.0 11 5825.0 12 8376.0 13 11084.0 14 14056.0 15 18186.0 16 22348.0 17 27023.0 18 24748.0 19 20728.0 20 21198.0 21 22605.0 22 24602.0 23 28211.0 24 33257.0 25 40127.0 26 49172.0 27 61001.0 28 74920.0 29 91189.0 30 111008.0 31 134616.0 32 165704.0 33 205326.0 34 262005.0 35 342335.0 36 458040.0 37 516834.0 38 242210.0 39 8923.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.648308850933 19.008012481471997 14.24647108255941 23.097207585035594 2 16.037085235656683 21.23267933361321 37.69138120627876 25.03885422445134 3 21.221990564656856 23.47918372853561 29.500690442875634 25.798135263931897 4 13.338812342833192 16.36587777661358 36.432051550712494 33.86325832984074 5 13.695949149567646 38.01735835514097 33.842079963955186 14.444612531336197 6 32.97752514668902 35.93975927284158 16.257858340318524 14.82485724015088 7 29.550502933780386 32.45445436923722 19.709012206621964 18.286030490360435 8 27.1812788139145 33.50776665968148 18.87527504191115 20.435679484492873 9 26.006784367141663 17.247681789606034 20.149079264459345 36.59645457879296 10-11 25.79133487007544 25.801534340947192 26.6603199654233 21.746810823554068 12-13 27.329160616631288 23.149442272552914 26.916036450668468 22.605360660147337 14-15 23.11451348977472 25.112648766524043 25.994536802908247 25.77830094079299 16-17 22.586281725803143 28.464321246385367 26.39481061314874 22.554586414662747 18-19 22.423919056552588 27.74814028726273 27.923891470083746 21.904049186100938 20-21 23.485141188181053 25.822931946772844 27.77798878876781 22.91393807627829 22-23 22.825754364699115 26.021564947701865 28.06313135902129 23.08954932857772 24-25 23.783070515507124 26.14045801026823 27.822928672464375 22.25354280176027 26-27 23.13106963808184 25.85401009091912 28.680829578462312 22.33409069253672 28-29 22.294805091257132 27.582658108000363 27.94128842669428 22.18124837404823 30-31 22.70894079471912 26.245982773609338 27.7316566444065 23.313419787265044 32-33 22.59647146563277 26.485210723954527 28.03566355880795 22.88265425160475 34-35 23.85559589955904 26.071025237941033 27.68134909707326 22.39202976542667 36-37 23.494246332413464 25.630588477209947 28.30542832931463 22.56973686106196 38-39 22.85331842853967 26.427996781979086 28.309104857777424 22.409579931703828 40-41 23.013498358750194 26.504784591079133 27.84008251270843 22.641634537462245 42-43 22.65280551320088 26.751359283592997 27.08971279900023 23.506122404205893 44-45 23.233847624785533 25.99974132624327 27.276967557726817 23.489443491244387 46-47 23.96462103910349 26.881821828213127 26.896228809050204 22.25732832363318 48-49 23.79361288934686 26.755099855700138 26.76150117695904 22.689786077993958 50-51 22.648917717379472 26.97171240742908 27.723019471509808 22.656350403681635 52-53 23.70228774557546 27.361528108415094 26.30492392708565 22.631260218923792 54-55 22.763123276969 26.548892209456394 26.999804684270405 23.688179829304204 56-57 23.591560538626176 26.641206962246162 26.720965711270058 23.04626678785761 58-59 24.491082292483775 26.239791431598704 26.331547772087927 22.937578503829595 60-61 23.32310642880162 26.678318220047004 27.778196257005344 22.220379094146036 62-63 22.942079360962435 26.797194910125516 27.518280110767908 22.74244561814414 64-65 22.696765731474574 27.665387521911526 26.801420350109588 22.836426396504308 66-67 23.1011171276424 26.895657586641487 26.324700154846457 23.67852513086965 68-69 22.90094094819104 26.76854678398859 26.908721444038026 23.421790823782345 70-71 23.58821549416878 26.73844610386506 26.559455914354512 23.113882487611644 72-73 23.40100783214585 26.13652884010897 27.434350115255658 23.028113212489522 74-75 22.69662005257347 27.970666127048617 26.544804015177416 22.787909805200503 76-77 23.22683978587451 26.66937022174336 26.480994537170638 23.622795455211495 78-79 23.262295063178332 26.898055571519397 26.688888332201127 23.150761033101144 80-81 23.752965231691295 26.836020210816162 26.532320882157556 22.878693675334986 82-83 23.25770732581167 26.587371634630657 27.190631968458394 22.964289071099277 84-85 22.90932435442242 27.120432481297573 26.91722784859879 23.053015315681215 86-87 23.185220114973582 27.23794881366977 26.43349395578216 23.143337115574482 88-89 22.77440891482867 27.386734711092092 25.82209291641218 24.01676345766706 90-91 23.142179203597806 26.47348361751309 26.431452881021894 23.95288429786721 92-93 23.576693079473426 26.990029076741802 26.620326206488265 22.81295163729651 94-95 23.125281889409933 26.711095330945128 26.695962966978364 23.467659812666575 96-97 22.366469608291194 26.665962301733558 27.409001783845145 23.558566306130103 98-99 22.74145715950021 27.259492936852663 26.009968804077683 23.989081099569443 100-101 22.320995906090737 26.560379018346413 26.61186824132276 24.506756834240086 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 248.0 1 199.0 2 167.5 3 209.0 4 209.5 5 210.0 6 344.0 7 696.0 8 1094.5 9 1300.5 10 1277.0 11 1202.0 12 1273.5 13 1440.5 14 1606.5 15 1723.0 16 1944.0 17 2190.0 18 2390.0 19 2702.5 20 3105.0 21 3562.0 22 4176.5 23 5097.0 24 6357.0 25 8050.0 26 10450.5 27 13706.0 28 17423.5 29 21752.5 30 27357.0 31 34164.5 32 42660.5 33 48237.5 34 51965.0 35 56973.5 36 63175.0 37 70236.5 38 74620.5 39 76622.5 40 79076.0 41 85043.0 42 90621.0 43 95265.5 44 100471.0 45 105906.0 46 110410.0 47 131799.0 48 177033.5 49 174398.0 50 134014.5 51 116354.5 52 108751.0 53 106865.5 54 105869.0 55 98698.5 56 89285.5 57 80463.0 58 74078.0 59 66605.5 60 56034.0 61 46781.5 62 38932.0 63 30383.0 64 22165.0 65 17023.0 66 12845.5 67 9987.5 68 7420.0 69 4945.5 70 3627.5 71 2629.5 72 1979.5 73 1681.5 74 1360.5 75 1065.5 76 785.5 77 492.5 78 310.5 79 216.5 80 143.0 81 93.5 82 63.0 83 41.0 84 27.0 85 17.0 86 10.5 87 5.5 88 4.0 89 2.0 90 1.5 91 1.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019449392288348702 2 0.011722024308466052 3 0.007956569572506286 4 0.0 5 0.003405280804694049 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0011296364207879295 14-15 2.4557313495389774E-4 16-17 1.637154233025985E-5 18-19 0.001522553436714166 20-21 0.0 22-23 1.1460079631181894E-4 24-25 0.0 26-27 0.0015389249790444257 28-29 0.0032579369237217097 30-31 0.0014734388097233865 32-33 0.00859505972338642 34-35 0.0021937866722548196 36-37 9.168063704945515E-4 38-39 9.495494551550712E-4 40-41 1.637154233025985E-4 42-43 0.0056809251886001675 44-45 0.001309723386420788 46-47 1.637154233025985E-4 48-49 7.530909471919531E-4 50-51 8.185771165129925E-5 52-53 1.1460079631181894E-4 54-55 0.0016535257753562448 56-57 0.016256941533948032 58-59 0.028977629924559933 60-61 0.02278918692372171 62-63 0.015978625314333614 64-65 0.019891423931265716 66-67 0.0028486483654652137 68-69 0.00108052179379715 70-71 6.54861693210394E-5 72-73 0.0 74-75 0.0022265297569153395 76-77 0.0025048459765297567 78-79 0.009937526194467729 80-81 0.01854895746018441 82-83 0.019285676865046102 84-85 0.0327430846605197 86-87 0.0109689333612741 88-89 0.044808911357921206 90-91 0.05081726739312657 92-93 0.031187788139145012 94-95 0.03343068943839061 96-97 0.05517209765297569 98-99 0.05756234283319363 100-101 0.0649950230511316 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3054080.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.453698889793976 #Duplication Level Percentage of deduplicated Percentage of total 1 70.90028231479207 31.517798012231513 2 14.991476823033606 13.328531932089223 3 5.6134142183294475 7.486110762159163 4 2.771006842330034 4.927260151619926 5 1.4882655833913536 3.3079455056061398 6 0.925444629848381 2.468366214867406 7 0.6162052858491468 1.9174822962006182 8 0.45057848304488674 1.6023904169198029 9 0.3312352166154068 1.3252167523005276 >10 1.691730749109604 13.70157231926212 >50 0.13501467531676406 4.147624575384418 >100 0.07606908400923193 6.184498800092824 >500 0.00537424512790192 1.6596048276420816 >1k 0.002944791850814566 2.4837618817642917 >5k 7.361979627036415E-4 2.2195263238365914 >10k+ 2.2085938881109243E-4 1.7223092280234193 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 29699 0.9724368713327745 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12221 0.4001532376362112 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10707 0.35058020746018437 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9269 0.3034956517183571 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8210 0.26882072506286675 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7594 0.24865098491198662 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7564 0.24766869237217098 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6755 0.22117953688181058 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6071 0.1987832669740151 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5906 0.19338065800502932 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5745 0.18810902137468566 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5560 0.18205155071248952 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5146 0.16849591366303437 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4641 0.15196065590947191 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3607 0.11810430637049454 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3313 0.10847783948030175 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3235 0.10592387887678123 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3071 0.10055401299245599 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.54861693210394E-5 0.0 0.0 0.0 0.0 6 6.54861693210394E-5 0.0 0.0 0.0 0.0 7 1.637154233025985E-4 0.0 0.0 0.0 0.0 8 1.9645850796311818E-4 0.0 0.0 0.0 0.0 9 1.9645850796311818E-4 0.0 0.0 3.27430846605197E-5 0.0 10-11 1.9645850796311818E-4 0.0 0.0 3.27430846605197E-5 0.0 12-13 1.9645850796311818E-4 0.0 0.0 3.27430846605197E-5 0.0 14-15 1.9645850796311818E-4 0.0 0.0 3.27430846605197E-5 6.54861693210394E-5 16-17 2.1283005029337803E-4 0.0 0.0 4.9114626990779544E-5 1.1460079631181894E-4 18-19 2.619446772841576E-4 0.0 0.0 9.822925398155909E-5 1.637154233025985E-4 20-21 2.946877619446773E-4 0.0 0.0 1.309723386420788E-4 1.8008696563285833E-4 22-23 2.946877619446773E-4 0.0 0.0 2.1283005029337803E-4 2.1283005029337803E-4 24-25 3.27430846605197E-4 0.0 0.0 4.2566010058675606E-4 2.2920159262363788E-4 26-27 3.27430846605197E-4 0.0 0.0 5.238893545683152E-4 2.2920159262363788E-4 28-29 5.730039815590947E-4 0.0 0.0 0.0011132648784576698 2.2920159262363788E-4 30-31 5.893755238893546E-4 0.0 0.0 0.0027831621961441743 2.4557313495389774E-4 32-33 5.893755238893546E-4 0.0 0.0 0.004616774937133278 3.27430846605197E-4 34-35 6.057470662196144E-4 0.0 0.0 0.007252593252305113 3.601739312657167E-4 36-37 6.876047778709137E-4 0.0 0.0 0.0109689333612741 3.601739312657167E-4 38-39 7.858340318524727E-4 0.0 0.0 0.016649858549874268 3.601739312657167E-4 40-41 8.349486588432523E-4 0.0 0.0 0.028437369027661356 3.601739312657167E-4 42-43 9.168063704945515E-4 0.0 0.0 0.03937355930427494 3.765454735959765E-4 44-45 0.0010477787091366304 0.0 0.0 0.05351857187761945 4.092885582564962E-4 46-47 0.0011132648784576698 0.0 0.0 0.07941835184409052 4.747747275775356E-4 48-49 0.0011787510477787092 0.0 0.0 0.0988022579631182 4.911462699077955E-4 50-51 0.0011787510477787092 0.0 0.0 0.12442372170997484 4.911462699077955E-4 52-53 0.0011787510477787092 0.0 0.0 0.15307392078792958 5.893755238893546E-4 54-55 0.0012114941324392289 0.0 0.0 0.18815813600167647 6.221186085498743E-4 56-57 0.0012442372170997486 0.0 0.0 0.2543973962699078 6.221186085498743E-4 58-59 0.0012442372170997486 0.0 0.0 0.31690394488683993 6.221186085498743E-4 60-61 0.0012442372170997486 0.0 0.0 0.3729437342833194 6.384901508801341E-4 62-63 0.0012442372170997486 0.0 0.0 0.43541753981559095 6.876047778709137E-4 64-65 0.0012442372170997486 0.0 0.0 0.5026882072506287 6.876047778709137E-4 66-67 0.001309723386420788 1.637154233025985E-5 0.0 0.6003608287929589 6.876047778709137E-4 68-69 0.0013752095557418274 3.27430846605197E-5 0.0 0.7180067319782062 6.876047778709137E-4 70-71 0.0013752095557418274 3.27430846605197E-5 0.0 0.8108824916177704 7.039763202011736E-4 72-73 0.001424324182732607 3.27430846605197E-5 0.0 0.9099303227158424 7.53090947191953E-4 74-75 0.0014734388097233865 3.27430846605197E-5 0.0 1.0444880291282481 7.53090947191953E-4 76-77 0.0014734388097233865 3.27430846605197E-5 0.0 1.1978730092204526 7.53090947191953E-4 78-79 0.0014734388097233865 3.27430846605197E-5 0.0 1.3742272632020116 7.694624895222129E-4 80-81 0.0018827273679798825 3.27430846605197E-5 0.0 1.554739888935457 8.185771165129924E-4 82-83 0.0019318419949706622 3.27430846605197E-5 0.0 1.7517877724224644 9.495494551550712E-4 84-85 0.0020791858759430007 3.27430846605197E-5 0.0 2.004597129086337 9.495494551550712E-4 86-87 0.0022101582145850796 3.27430846605197E-5 0.0 2.2614338851634534 0.0010314071668063706 88-89 0.002259272841575859 3.27430846605197E-5 0.0 2.57057771898575 0.0011132648784576698 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGGT 1095 0.0 26.889688 4 TAAGGTG 1250 0.0 25.454994 5 GTACATG 12265 0.0 24.79125 1 TACATGG 12910 0.0 22.848562 2 ACATGGG 12765 0.0 22.174559 3 CATGGGG 6370 0.0 20.278587 4 GAGTACT 8660 0.0 20.208208 12-13 GGTAAGG 1390 0.0 19.475544 3 GTACTTT 9170 0.0 19.00662 14-15 AGTACTT 9195 0.0 18.645056 12-13 AGGTAAG 1470 0.0 17.772106 2 GTACTAG 605 0.0 17.276402 1 AGAGTAC 17750 0.0 16.601393 10-11 TACCTGG 2150 0.0 16.569765 2 TACTTTT 10285 0.0 16.32275 14-15 GTATAGA 645 0.0 16.204996 1 ACTTTTT 10910 0.0 16.192965 16-17 GTACCTG 2450 0.0 15.9013405 1 AGTACAT 9735 0.0 15.760141 2 TAGACTG 1095 0.0 15.613368 5 >>END_MODULE