FastQCFastQC Report
Thu 26 May 2016
SRR936579_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936579_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670225
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT98580.36918237227199957No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83030.31094757932383976No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65980.24709528223277066No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT62910.23559812375361627No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45270.16953627503300284No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38550.14436985647276915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37850.14174835453941148No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT31420.11766798678014025No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27960.10471027722382945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG122650.025.4436451
TACATGG123450.024.5087812
ACATGGG125050.022.9042023
GAGTACT92050.022.55595612-13
GTACTTT96550.021.28382314-15
TACCTGG24250.020.5660532
GTACCTG25900.019.9897921
AGTACTT98200.019.93376412-13
CATGGGG58350.019.5370984
GTATCAA253200.018.8906671
AGAGTAC171650.018.78078310-11
GTGTACG3105.5933924E-918.3865871
GTGTAGC15100.018.2445261
ACTTTTT118000.017.57641416-17
TACCGTC2453.7610844E-617.451117
CATGGGA72650.017.3914454
TACTTTT110500.017.37140314-15
AGTACAT93350.017.2996772
GGTCGAC1650.001463302317.2748367
TAGACTG11150.017.0405245
Produced by FastQC (version 0.11.5)