##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936579_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2670225 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3103472553811 31.0 31.0 34.0 28.0 34.0 2 31.386232995346834 31.0 31.0 34.0 28.0 34.0 3 31.39321293149454 31.0 31.0 34.0 28.0 34.0 4 35.028691215159775 37.0 35.0 37.0 32.0 37.0 5 35.03800129202595 37.0 35.0 37.0 32.0 37.0 6 35.03229690381897 37.0 35.0 37.0 32.0 37.0 7 34.93843852109841 37.0 35.0 37.0 32.0 37.0 8 34.896081041859766 37.0 35.0 37.0 32.0 37.0 9 36.37246599069367 39.0 37.0 39.0 32.0 39.0 10-11 36.23707852334541 39.0 35.0 39.0 32.0 39.0 12-13 36.128171783276684 39.0 35.0 39.0 31.0 39.0 14-15 37.193185405724236 39.0 36.5 41.0 31.0 41.0 16-17 37.087842972034196 39.0 36.0 41.0 31.0 41.0 18-19 37.05378685316781 39.0 36.0 41.0 31.0 41.0 20-21 36.9095377730341 39.0 36.0 41.0 30.5 41.0 22-23 37.02969487589997 39.0 36.0 41.0 31.0 41.0 24-25 37.06236740349596 39.0 36.0 41.0 31.0 41.0 26-27 36.968821915756166 39.0 36.0 41.0 30.5 41.0 28-29 36.82873184843974 39.0 36.0 41.0 30.0 41.0 30-31 36.628023668417455 39.0 36.0 41.0 30.0 41.0 32-33 36.429120954226704 39.0 35.0 41.0 30.0 41.0 34-35 36.233951258789055 39.0 35.0 40.0 30.0 41.0 36-37 36.02489752736193 38.5 35.0 40.0 29.0 41.0 38-39 35.82641856023369 38.0 35.0 40.0 28.5 41.0 40-41 35.61829321499125 38.0 35.0 40.0 27.0 41.0 42-43 35.37028452658484 38.0 34.5 40.0 26.5 41.0 44-45 35.10121338089487 38.0 34.0 40.0 26.0 41.0 46-47 34.911714181389215 38.0 34.0 40.0 24.5 41.0 48-49 34.747571459333955 38.0 34.0 40.0 24.0 41.0 50-51 33.78144594556639 37.5 32.5 39.5 23.5 40.5 52-53 33.889028827158754 37.5 33.0 39.5 23.0 40.5 54-55 34.327550861818764 38.0 34.0 40.0 23.0 41.0 56-57 34.21264687432706 38.0 33.5 40.0 23.0 41.0 58-59 33.971739647407986 38.0 33.0 40.0 22.5 41.0 60-61 33.66861987285716 37.0 33.0 40.0 22.0 41.0 62-63 33.300331619994566 37.0 32.5 40.0 20.5 41.0 64-65 32.93857764795102 36.0 32.0 39.0 19.5 41.0 66-67 32.46580475428101 36.0 31.0 39.0 18.0 41.0 68-69 32.06688069357451 35.0 31.0 39.0 16.0 40.0 70-71 31.64980928573435 35.0 31.0 38.0 13.0 40.0 72-73 31.245078785495604 35.0 30.5 37.5 10.0 39.5 74-75 30.804245147880795 35.0 30.0 37.0 7.5 39.0 76-77 30.338134801374416 34.5 30.0 36.0 7.0 39.0 78-79 29.831029220384053 34.0 29.0 36.0 2.0 38.5 80-81 29.348217285060247 34.0 29.0 35.0 2.0 37.0 82-83 28.923980563435663 34.0 29.0 35.0 2.0 37.0 84-85 28.53547772191482 34.0 28.0 35.0 2.0 36.0 86-87 28.166275875628457 34.0 27.0 35.0 2.0 36.0 88-89 27.74687133106761 33.5 26.5 35.0 2.0 36.0 90-91 27.311789081444445 33.0 26.0 35.0 2.0 35.0 92-93 26.82912994223333 33.0 25.0 35.0 2.0 35.0 94-95 26.30400771470569 33.0 24.5 35.0 2.0 35.0 96-97 25.684344577704124 32.5 22.0 35.0 2.0 35.0 98-99 24.891381063393535 32.0 10.5 35.0 2.0 35.0 100-101 21.925671806682956 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4539788722126552 1101 2 0.37927594296640166 1101 3 0.4163667503492121 1101 4 0.4663823364991231 1101 5 0.435939592388884 1101 6 0.4640516897416731 1101 7 0.4550336878822776 1101 8 0.447888216408586 1101 9 0.4377796158096885 1101 10-11 0.6311513355922145 1101 12-13 0.7182005554294975 1101 14-15 0.7373687114082017 1101 16-17 0.880427976197069 1101 18-19 0.7692643566816528 1101 20-21 0.7099889439309592 1101 22-23 0.5595645779587386 1101 24-25 0.5652967479967046 1101 26-27 0.7026357375044725 1101 28-29 0.582393676576558 1101 30-31 0.730343691396655 1101 32-33 0.4811150490910663 1101 34-35 0.33837089043316126 1101 36-37 0.23840575180847878 1101 38-39 0.18545027042424067 1101 40-41 0.27282981602967027 1101 42-43 0.37318344289119665 1101 44-45 0.28405743186475263 1101 46-47 0.3179892193114924 1101 48-49 0.5331039029525684 1101 50-51 0.3234857007428005 1101 52-53 0.46177355467892056 1101 54-55 0.34107817947248975 1101 56-57 0.32246367937301557 1101 58-59 0.3568489882897836 1101 60-61 0.4191598888488386 1101 62-63 0.46152015593255413 1101 64-65 0.327045863695254 1101 66-67 0.6093665817677518 1101 68-69 0.45811426729799365 1101 70-71 0.45452862066980515 1101 72-73 0.37123390183441884 1101 74-75 0.2590321502673163 1101 76-77 0.35004527374387706 1101 78-79 0.37630887312543493 1101 80-81 0.5392105271901855 1101 82-83 0.38949457722090486 1101 84-85 0.27022997092919 1101 86-87 0.425336668111985 1101 88-89 0.437721590707298 1101 90-91 0.599501627705191 1101 92-93 0.5304203293087397 1101 94-95 0.7290093845103236 1101 96-97 0.5630879369256157 1101 98-99 0.4799109373443784 1101 100-101 0.7244119522632424 1102 1 0.2422227173654825 1102 2 0.26306724418626004 1102 3 0.3555856375570734 1102 4 0.3705610730494442 1102 5 0.29451151972624956 1102 6 0.3441124182724451 1102 7 0.3952027544191665 1102 8 0.3851089868356965 1102 9 0.3932366138735688 1102 10-11 0.543697966428347 1102 12-13 0.5489492937783993 1102 14-15 0.5608310311534339 1102 16-17 0.3998848049828183 1102 18-19 0.4281995972153041 1102 20-21 0.3745335857490062 1102 22-23 0.2729117885724719 1102 24-25 0.30271049549720885 1102 26-27 0.14491014794047175 1102 28-29 -0.05107795346607702 1102 30-31 0.296640467706176 1102 32-33 0.3171250576607747 1102 34-35 0.5081022671933866 1102 36-37 0.06757475486606523 1102 38-39 0.0986391001833411 1102 40-41 0.04953354755455308 1102 42-43 -0.001416002722912424 1102 44-45 0.1019015690223597 1102 46-47 0.19502542367251152 1102 48-49 0.24042528807090235 1102 50-51 0.2735837762620079 1102 52-53 0.4446718608395841 1102 54-55 0.36597428501740836 1102 56-57 0.47076523778984125 1102 58-59 0.4166974314599443 1102 60-61 0.2561142578116957 1102 62-63 0.2501174220906819 1102 64-65 0.2819571513728718 1102 66-67 0.29087543184022735 1102 68-69 0.3786022115208496 1102 70-71 0.3488882732264358 1102 72-73 0.06614968596247905 1102 74-75 0.18864656641645183 1102 76-77 0.28708227159615873 1102 78-79 0.18771689690901283 1102 80-81 0.5268903058590233 1102 82-83 0.3999791358810718 1102 84-85 0.007494700771868423 1102 86-87 0.14371027553482918 1102 88-89 0.11129864928811273 1102 90-91 0.04909297970849025 1102 92-93 0.13958409532403593 1102 94-95 -0.04992612052041068 1102 96-97 0.11835745945547771 1102 98-99 0.11006074849345993 1102 100-101 0.21373478682560787 1103 1 0.29334067099126315 1103 2 0.18709352082959185 1103 3 0.21250515363409406 1103 4 0.4307557032935918 1103 5 0.38278023637332126 1103 6 0.43269424931550304 1103 7 0.41156417789338207 1103 8 0.4385742581841683 1103 9 0.1934034695184721 1103 10-11 0.4785030241100472 1103 12-13 0.0879433747720455 1103 14-15 0.4182504380044989 1103 16-17 0.5851844481780404 1103 18-19 0.6220434145062939 1103 20-21 0.6963064269760864 1103 22-23 0.3310523987814591 1103 24-25 0.45547193561538535 1103 26-27 0.36515216144287876 1103 28-29 0.3579175318612329 1103 30-31 0.371190352758866 1103 32-33 0.23145373415430726 1103 34-35 0.5385226310718423 1103 36-37 0.5891752271382771 1103 38-39 0.5209684736424762 1103 40-41 0.3329567929176491 1103 42-43 0.2954859851894085 1103 44-45 0.45529235867397233 1103 46-47 0.7659581612909747 1103 48-49 0.6861172084711953 1103 50-51 0.6689486429543408 1103 52-53 0.7098289381611522 1103 54-55 0.6561710477682396 1103 56-57 0.5616731135011079 1103 58-59 0.6437840632436576 1103 60-61 0.5565541783368104 1103 62-63 0.6171080390612715 1103 64-65 0.44556992976901455 1103 66-67 0.5934654912383408 1103 68-69 0.5679438390915621 1103 70-71 0.6470489081932982 1103 72-73 0.33277018299257577 1103 74-75 0.418925820767285 1103 76-77 0.3618843645734522 1103 78-79 0.41525249936815456 1103 80-81 0.4561821576029992 1103 82-83 0.10193781726377082 1103 84-85 0.296129740457296 1103 86-87 0.4151649051548283 1103 88-89 0.33420104289683294 1103 90-91 0.31908054875489356 1103 92-93 0.23620277192159733 1103 94-95 0.3993883045869957 1103 96-97 0.34998430199326336 1103 98-99 0.3961248693285704 1103 100-101 0.5179816478797932 1104 1 0.4229069340696725 1104 2 0.41846841584867533 1104 3 0.32459943500246524 1104 4 0.4555451200866756 1104 5 0.4517925799846978 1104 6 0.5213192092192784 1104 7 0.5563897828997355 1104 8 0.5173362125140955 1104 9 0.557827652470138 1104 10-11 0.6940627503964194 1104 12-13 0.6202725893723979 1104 14-15 0.42949338322838315 1104 16-17 0.4098772286593544 1104 18-19 0.4252405627163611 1104 20-21 0.32658330137010694 1104 22-23 0.3945986943379509 1104 24-25 0.4542426201757408 1104 26-27 0.20454387004946284 1104 28-29 0.43727302238572463 1104 30-31 0.6437136059047219 1104 32-33 0.5114160311649911 1104 34-35 0.5563380509160325 1104 36-37 0.2689654370553072 1104 38-39 0.30603545927973386 1104 40-41 0.16483217207836276 1104 42-43 0.4103054766876184 1104 44-45 0.39826525388998135 1104 46-47 0.19780203951022912 1104 48-49 0.4822447794968525 1104 50-51 0.45302910401803587 1104 52-53 0.43196545937980346 1104 54-55 0.4626291247941694 1104 56-57 0.4233918210813812 1104 58-59 0.49143620836389346 1104 60-61 0.4777011240949207 1104 62-63 0.696555216206761 1104 64-65 0.7432643339409211 1104 66-67 0.6621798933343612 1104 68-69 0.478527515374104 1104 70-71 0.5230854497120845 1104 72-73 0.42594379136941285 1104 74-75 0.47664209531852464 1104 76-77 0.4289878770084279 1104 78-79 0.40181926264268597 1104 80-81 0.5682331825143443 1104 82-83 0.45519314034149616 1104 84-85 0.4627018225159141 1104 86-87 0.6157175210610646 1104 88-89 0.49966621835536884 1104 90-91 0.19354492340893614 1104 92-93 0.08704762759811047 1104 94-95 0.286511033342979 1104 96-97 0.5657069200425902 1104 98-99 0.6140195375622142 1104 100-101 0.5955896522124782 1105 1 0.31035890631918406 1105 2 0.18775032749400822 1105 3 0.32496757854609726 1105 4 0.3291537660208519 1105 5 0.28655996505057146 1105 6 0.3973813131134847 1105 7 0.4385079620797896 1105 8 0.46316026942989197 1105 9 0.6371410359915757 1105 10-11 0.45196326174790613 1105 12-13 0.3622757809630457 1105 14-15 0.45910744264225656 1105 16-17 0.4430248581286875 1105 18-19 0.3645918180141834 1105 20-21 0.31223237990931096 1105 22-23 0.12601854614470653 1105 24-25 0.24796780834855525 1105 26-27 0.07075995261349277 1105 28-29 -0.023390063738474964 1105 30-31 -0.054851904484344516 1105 32-33 -0.2193570453067153 1105 34-35 -6.532511906414129E-4 1105 36-37 0.06737704585561488 1105 38-39 -0.0686072129538573 1105 40-41 -0.11717490179277235 1105 42-43 -0.136214958861693 1105 44-45 -0.0051993340340601435 1105 46-47 0.03207556288930391 1105 48-49 0.011083577027783065 1105 50-51 0.05192249123285109 1105 52-53 0.24041812633605275 1105 54-55 0.050021364640940646 1105 56-57 -0.014460261643769456 1105 58-59 0.015854243554336733 1105 60-61 0.13412467525031957 1105 62-63 0.06353754976782255 1105 64-65 0.032120929599997794 1105 66-67 0.16236081672435176 1105 68-69 0.20568966930587607 1105 70-71 0.1478397537943117 1105 72-73 -0.017827520597119673 1105 74-75 0.1267541180007683 1105 76-77 0.17534994895199318 1105 78-79 0.22327355231835 1105 80-81 0.3045720552264619 1105 82-83 0.13446710395119865 1105 84-85 0.26659063860601506 1105 86-87 -0.005742544266773564 1105 88-89 0.07267800709113104 1105 90-91 0.11283865234259949 1105 92-93 -0.3030051251678252 1105 94-95 -0.2601898913367613 1105 96-97 -0.001946594240916255 1105 98-99 0.2136063830149908 1105 100-101 0.2503661111319069 1106 1 0.4418415351133973 1106 2 0.3931887963134244 1106 3 0.4230319556583382 1106 4 0.3900245258691726 1106 5 0.22308192319668052 1106 6 0.40408197587924377 1106 7 0.5118219092078604 1106 8 0.5577270326514991 1106 9 0.6226883246273474 1106 10-11 0.6716203274533825 1106 12-13 0.6724930057933634 1106 14-15 0.5995710966045849 1106 16-17 0.6419853301140961 1106 18-19 0.4657502892361336 1106 20-21 0.3338398797138069 1106 22-23 0.6110963890704255 1106 24-25 0.4854341111837357 1106 26-27 0.3939582251771725 1106 28-29 0.5408549754183198 1106 30-31 0.6007076258000481 1106 32-33 0.4156019594949285 1106 34-35 0.40686804559450707 1106 36-37 0.34352771049628217 1106 38-39 0.38021291755072895 1106 40-41 0.23504101137702804 1106 42-43 0.3647189549295362 1106 44-45 0.31102743744598627 1106 46-47 0.22451589411621597 1106 48-49 0.14350725421461874 1106 50-51 -0.09345972374342182 1106 52-53 0.011901766141996006 1106 54-55 0.1323303849119455 1106 56-57 0.19071538837096824 1106 58-59 0.2363736005022048 1106 60-61 0.2971194004016908 1106 62-63 0.26004626299656053 1106 64-65 0.24494702927712808 1106 66-67 0.2240989007509242 1106 68-69 0.3121258098117323 1106 70-71 0.11387473723373986 1106 72-73 -0.008535670159162123 1106 74-75 0.24804538351202865 1106 76-77 0.19579423219500214 1106 78-79 0.013907955543235317 1106 80-81 0.20969236506947908 1106 82-83 0.1915447446745766 1106 84-85 0.46498869383534824 1106 86-87 0.3203711255299382 1106 88-89 0.2591574618173276 1106 90-91 0.057592115361966734 1106 92-93 0.17219931434618374 1106 94-95 0.19608119652624367 1106 96-97 0.265963273952579 1106 98-99 0.1664195120974057 1106 100-101 0.01776389192797012 1107 1 0.11073033400373689 1107 2 0.17410480078173762 1107 3 0.2567444740517928 1107 4 0.29395463012265566 1107 5 0.3271448244677799 1107 6 0.28308875946309797 1107 7 0.35311461205371586 1107 8 0.4993267477720593 1107 9 0.5426990518492687 1107 10-11 0.44469681551792917 1107 12-13 0.28430330730702025 1107 14-15 0.05456112094616827 1107 16-17 0.17446206255873165 1107 18-19 0.22788038244022601 1107 20-21 0.3238880444410981 1107 22-23 0.29784273394766103 1107 24-25 0.31129140124198784 1107 26-27 0.36345864361369706 1107 28-29 0.3344639516163852 1107 30-31 0.4081800275372274 1107 32-33 0.2201926682187505 1107 34-35 0.2644039360134869 1107 36-37 0.37813842952757426 1107 38-39 0.3455267018380468 1107 40-41 0.44181984194874957 1107 42-43 0.48683531919541423 1107 44-45 0.5079721871481055 1107 46-47 0.5283636610423557 1107 48-49 0.5819960636325163 1107 50-51 0.5311023699797843 1107 52-53 0.7332883253730813 1107 54-55 0.6999962283187813 1107 56-57 0.5018030658264578 1107 58-59 0.5312251238845676 1107 60-61 0.4625494756703503 1107 62-63 0.45715561928724213 1107 64-65 0.4440722206989349 1107 66-67 0.5167930114539061 1107 68-69 0.6547240777329435 1107 70-71 0.48019064779348497 1107 72-73 0.2894984552483706 1107 74-75 0.449037061633657 1107 76-77 0.3345388681586279 1107 78-79 0.29632022369156275 1107 80-81 0.34826689243245923 1107 82-83 0.5109419918761198 1107 84-85 0.2870684839012547 1107 86-87 0.4692827991773534 1107 88-89 0.31496017305006774 1107 90-91 0.19639784067634025 1107 92-93 0.210848793861512 1107 94-95 0.22748883697190792 1107 96-97 0.2336539274634255 1107 98-99 0.3780027903822578 1107 100-101 0.4077544500214465 1108 1 0.15906477653992823 1108 2 0.11001701267690578 1108 3 0.14552959368523233 1108 4 0.16033952461856416 1108 5 0.08366746836729533 1108 6 0.030262576443142564 1108 7 0.16557043286744744 1108 8 0.12255100003665831 1108 9 0.2322033049864558 1108 10-11 0.2530416948782701 1108 12-13 0.050354550080598415 1108 14-15 0.19530343064115385 1108 16-17 0.26467217759793726 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2316 94-95 -3.4369232742206 2316 96-97 -3.693813634788672 2316 98-99 -4.886473090848728 2316 100-101 -5.866503986102918 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6360.0 3 2628.0 4 2617.0 5 2957.0 6 3308.0 7 3957.0 8 5083.0 9 6417.0 10 7896.0 11 9868.0 12 12124.0 13 14578.0 14 15992.0 15 18947.0 16 22786.0 17 27774.0 18 23061.0 19 18996.0 20 19462.0 21 20856.0 22 23212.0 23 26028.0 24 29709.0 25 34245.0 26 39853.0 27 46943.0 28 55151.0 29 65443.0 30 79261.0 31 95840.0 32 119044.0 33 149924.0 34 195452.0 35 261497.0 36 359127.0 37 456436.0 38 341575.0 39 45805.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.81503246749598 18.24371016003964 14.200339263515763 22.74091810894861 2 15.381595989090302 19.935368801588663 40.07872151878822 24.604313690532816 3 19.93463232179236 24.251625858063143 29.38923620722138 26.424505612923117 4 12.750655961524416 16.197651835662207 37.39795373715467 33.653738465658705 5 13.73806393125124 37.44559700010864 33.89098047148642 14.925358597153698 6 31.988378789417265 35.540349198344046 17.533317871358115 14.937954140880574 7 28.893771022522774 32.62807891828364 20.359183881368647 18.118966177824937 8 26.63030774348416 33.76221578904782 19.655770227788317 19.9517062396797 9 26.339160780214776 17.12261615088798 20.533929752197704 36.004293316699545 10-11 25.17897434312384 26.327946822370645 26.986878867661922 21.506199966843596 12-13 26.52859278938789 23.730684388732396 27.432658641389462 22.308064180490256 14-15 23.070196025472427 25.157741720327355 26.344492146300702 25.427570107899513 16-17 22.10164187317917 28.585653005056095 26.851682237283995 22.46102288448074 18-19 21.987358844267156 27.30657452848512 29.004704239021866 21.70136238822586 20-21 23.040892395286598 25.943949887073554 28.93594339719853 22.079214320441327 22-23 22.421036742836105 26.08747356155574 29.032080456020324 22.45940923958783 24-25 22.61195129628704 26.217129366171243 29.104687366287717 22.066231971254002 26-27 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27.029292839825576 28.154315708617972 22.552215281427994 52-53 22.773438407026486 27.242141382092083 27.431608096396946 22.552812114484485 54-55 22.31010566361944 26.845892459281135 27.797587859825768 23.04641401727366 56-57 22.675113722733556 27.01340217710243 27.508439909287713 22.803044190876303 58-59 23.11477030732804 26.73712363007978 27.38467801678323 22.763428045808947 60-61 22.64538739887242 26.82920983228607 28.20121551587893 22.324187252962577 62-63 22.450739370547755 26.949448367460167 28.079902314817502 22.519909947174575 64-65 22.25523471828525 27.324085340391978 27.728642899916522 22.692037041406245 66-67 22.410722947570765 27.13391623413569 27.381223941858245 23.07413687643529 68-69 22.270170387915563 27.00111573774693 27.760027215308913 22.9686866590286 70-71 22.619829819426933 26.91610521541236 27.494558789337418 22.96950617582328 72-73 22.533788725211753 26.74121938872686 27.88958750852362 22.83540437753777 74-75 22.159539604309053 27.54750584036 27.473049679398326 22.81990487593262 76-77 22.409843910817422 27.068881715173532 27.423971260185787 23.097303113823262 78-79 22.46962201173432 27.08280443329864 27.47427320938258 22.973300345584455 80-81 22.656584105404757 27.203519138963113 27.315080476709674 22.824816278922462 82-83 22.49944967706582 27.136850092032727 27.68619883753062 22.677501393370832 84-85 22.38640039150547 27.237461065301666 27.429037292469232 22.94710125072363 86-87 22.418322932508257 27.276844242337976 27.349737419746333 22.95509540540743 88-89 22.30734768535531 27.269516019132535 27.00567390710705 23.417462388405106 90-91 22.392223962702356 26.941264977297784 27.36469994076706 23.301811119232795 92-93 22.74424894456286 26.935036767047137 27.48397646009942 22.836737828290584 94-95 22.31326828307239 26.823606570148765 27.570788135437684 23.292337011341157 96-97 21.975564185777515 26.819781937065308 27.85149425700681 23.353159620150368 98-99 22.068854530879026 27.090701886218397 27.21614877671484 23.62429480618774 100-101 21.96915597830354 26.788330591638353 27.424901011350972 23.817612418707135 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 177.0 1 147.0 2 174.0 3 235.0 4 218.0 5 242.0 6 439.5 7 891.5 8 1488.0 9 1864.5 10 1819.0 11 1721.0 12 1855.0 13 2070.5 14 2296.0 15 2497.0 16 2681.0 17 2882.0 18 3125.5 19 3438.5 20 3865.5 21 4543.0 22 5273.5 23 6283.5 24 7706.5 25 9485.5 26 12162.0 27 15515.0 28 18850.0 29 23138.0 30 28718.0 31 34684.0 32 41264.5 33 46513.0 34 51675.0 35 56477.0 36 61687.5 37 67838.5 38 71207.5 39 73503.5 40 76502.5 41 81348.5 42 85871.5 43 89762.5 44 94264.0 45 98983.5 46 101383.5 47 108586.0 48 126200.0 49 125322.0 50 107426.0 51 96529.0 52 90493.5 53 88299.5 54 85464.5 55 79743.5 56 73505.0 57 66546.5 58 61003.5 59 54340.0 60 45251.5 61 37092.0 62 30302.5 63 23537.0 64 17487.0 65 13662.0 66 10391.5 67 7990.0 68 5970.5 69 4070.0 70 2965.5 71 2252.0 72 1781.0 73 1483.0 74 1150.0 75 958.5 76 708.5 77 373.5 78 221.0 79 146.5 80 82.5 81 46.5 82 37.0 83 30.5 84 24.0 85 16.5 86 12.5 87 8.5 88 6.0 89 3.5 90 4.5 91 5.0 92 2.5 93 2.5 94 2.5 95 1.5 96 2.0 97 1.5 98 1.5 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0121338089486841 2 0.011497158479154378 3 0.01512981115823573 4 0.017976013257309777 5 0.029772771957419317 6 0.04913443623664673 7 0.0064414047505360035 8 0.027301070134539227 9 0.03595202651461955 10-11 0.0396034042075106 12-13 0.02937954666741567 14-15 0.028106245728356224 16-17 0.028986321377412017 18-19 0.022900691889260267 20-21 0.05439616511717177 22-23 0.012882809501072007 24-25 0.0029772771957419317 26-27 0.0015167261185855124 28-29 7.490005523879073E-5 30-31 0.0026027769195479783 32-33 0.002153376588115234 34-35 3.7450027619395366E-5 36-37 3.1832523476486065E-4 38-39 9.362506904848842E-5 40-41 0.0012920259528691404 42-43 0.003595202651461955 44-45 0.001722701270492187 46-47 9.362506904848841E-4 48-49 5.617504142909305E-4 50-51 0.0038011778033686296 52-53 0.0037075527343201414 54-55 0.0055426040876705145 56-57 9.175256766751866E-4 58-59 0.00670355494387177 60-61 0.0031083522924098155 62-63 5.430254004812329E-4 64-65 8.988006628654888E-4 66-67 0.017751313091593404 68-69 0.042655581458491326 70-71 0.05510771564194029 72-73 0.016346937055866078 74-75 0.03254407400125457 76-77 0.028368395921691995 78-79 0.05832841801720828 80-81 0.08186576037599827 82-83 0.050089411940941304 84-85 0.019867239652089242 86-87 0.021159265604958382 88-89 0.023818217565935454 90-91 0.0412699304365737 92-93 0.02677676974786769 94-95 0.014680410826802985 96-97 0.020110664831615315 98-99 0.05117546274190377 100-101 0.06978812646874327 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2670225.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.65103620883747 #Duplication Level Percentage of deduplicated Percentage of total 1 77.90279657781848 44.13274149699695 2 12.780366772106131 14.480420415376155 3 4.067161750438239 6.912267827738264 4 1.7634342943333567 3.9960152024073903 5 0.9413324130353498 2.6663728307708974 6 0.5668047962937364 1.9266047422907526 7 0.3615525254951476 1.4337627649255567 8 0.2631361453151129 1.1925548236880306 9 0.18872617747724094 0.9622380162436797 >10 1.0321490117419114 10.883580592723554 >50 0.08649533435481502 3.3955876772034306 >100 0.04193405847038848 4.326639879443832 >500 0.0025854126076988743 1.0093030512634529 >1k 0.0012595599883514648 1.5158135722693225 >5k 2.651705238634663E-4 1.1660971066587995 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9858 0.36918237227199957 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8303 0.31094757932383976 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6598 0.24709528223277066 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6291 0.23559812375361627 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4527 0.16953627503300284 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3855 0.14436985647276915 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3785 0.14174835453941148 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3142 0.11766798678014025 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2796 0.10471027722382945 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 7.490005523879073E-5 0.0 0.0 0.0 6 0.0 7.490005523879073E-5 0.0 0.0 0.0 7 0.0 7.490005523879073E-5 0.0 0.0 0.0 8 0.0 7.490005523879073E-5 0.0 0.0 0.0 9 0.0 7.490005523879073E-5 0.0 0.0 0.0 10-11 0.0 7.490005523879073E-5 0.0 1.8725013809697683E-5 0.0 12-13 0.0 1.4980011047758147E-4 0.0 3.7450027619395366E-5 0.0 14-15 0.0 1.4980011047758147E-4 0.0 3.7450027619395366E-5 0.0 16-17 0.0 1.4980011047758147E-4 0.0 7.490005523879073E-5 0.0 18-19 0.0 1.4980011047758147E-4 0.0 7.490005523879073E-5 0.0 20-21 0.0 1.4980011047758147E-4 0.0 9.362506904848843E-5 0.0 22-23 0.0 1.4980011047758147E-4 0.0 1.6852512428727916E-4 0.0 24-25 0.0 1.4980011047758147E-4 0.0 2.9960022095516293E-4 0.0 26-27 0.0 1.4980011047758147E-4 0.0 5.430254004812329E-4 0.0 28-29 0.0 1.4980011047758147E-4 0.0 9.737007181042796E-4 0.0 30-31 0.0 1.4980011047758147E-4 0.0 0.0022282766433540245 0.0 32-33 0.0 1.4980011047758147E-4 0.0 0.003745002761939537 0.0 34-35 0.0 1.4980011047758147E-4 0.0 0.004999578687189282 0.0 36-37 0.0 1.4980011047758147E-4 0.0 0.006984430151017237 0.0 38-39 0.0 1.4980011047758147E-4 0.0 0.010766882940576167 0.0 40-41 0.0 1.4980011047758147E-4 0.0 0.01439953561965752 0.0 42-43 0.0 1.4980011047758147E-4 0.0 0.017994738271119477 0.0 44-45 0.0 1.4980011047758147E-4 0.0 0.02037281502495108 0.0 46-47 0.0 1.4980011047758147E-4 0.0 0.023855667593554848 0.0 48-49 0.0 1.4980011047758147E-4 0.0 0.028667996142647152 0.0 50-51 0.0 1.4980011047758147E-4 0.0 0.03462255053413102 0.0 52-53 0.0 1.4980011047758147E-4 0.0 0.047093409731389674 0.0 54-55 0.0 1.4980011047758147E-4 0.0 0.05542604087670515 0.0 56-57 0.0 1.4980011047758147E-4 0.0 0.07656658146785383 0.0 58-59 0.0 1.4980011047758147E-4 0.0 0.10791225458528775 0.0 60-61 0.0 1.4980011047758147E-4 0.0 0.13369659860124147 0.0 62-63 0.0 1.4980011047758147E-4 0.0 0.14813358424851836 0.0 64-65 0.0 1.4980011047758147E-4 0.0 0.17446095366495334 0.0 66-67 0.0 1.4980011047758147E-4 0.0 0.23672162458219811 0.0 68-69 0.0 1.4980011047758147E-4 0.0 0.32471046447396756 0.0 70-71 0.0 1.6852512428727916E-4 0.0 0.401483021093728 0.0 72-73 0.0 1.8725013809697686E-4 0.0 0.49211208793266487 0.0 74-75 0.0 2.2470016571637222E-4 0.0 0.5975900907226919 0.0 76-77 0.0 2.2470016571637222E-4 0.0 0.6603750620266082 0.0 78-79 0.0 2.2470016571637222E-4 0.0 0.7546180565308167 0.0 80-81 0.0 2.2470016571637222E-4 0.0 0.8841202520386859 0.0 82-83 0.0 2.2470016571637222E-4 0.0 1.0005149378797666 0.0 84-85 0.0 2.2470016571637222E-4 0.0 1.1556891273207315 0.0 86-87 0.0 2.2470016571637222E-4 0.0 1.3733112378170378 0.0 88-89 0.0 2.2470016571637222E-4 0.0 1.67729311200367 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12265 0.0 25.443645 1 TACATGG 12345 0.0 24.508781 2 ACATGGG 12505 0.0 22.904202 3 GAGTACT 9205 0.0 22.555956 12-13 GTACTTT 9655 0.0 21.283823 14-15 TACCTGG 2425 0.0 20.566053 2 GTACCTG 2590 0.0 19.989792 1 AGTACTT 9820 0.0 19.933764 12-13 CATGGGG 5835 0.0 19.537098 4 GTATCAA 25320 0.0 18.890667 1 AGAGTAC 17165 0.0 18.780783 10-11 GTGTACG 310 5.5933924E-9 18.386587 1 GTGTAGC 1510 0.0 18.244526 1 ACTTTTT 11800 0.0 17.576414 16-17 TACCGTC 245 3.7610844E-6 17.45111 7 CATGGGA 7265 0.0 17.391445 4 TACTTTT 11050 0.0 17.371403 14-15 AGTACAT 9335 0.0 17.299677 2 GGTCGAC 165 0.0014633023 17.274836 7 TAGACTG 1115 0.0 17.040524 5 >>END_MODULE