FastQCFastQC Report
Thu 26 May 2016
SRR936579_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936579_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2670225
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT116700.437041822318344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94490.3538653109756668No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80510.3015101723637521No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52830.19784849591326573No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47700.1786366317445159No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45220.16934902489490586No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC35430.13268544785551778No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT34180.12800419440309338No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28740.1076313793781423No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC28570.10699472890861256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG123250.023.7471391
TACATGG125150.023.2317092
ACATGGG123200.022.6701283
GAGTACT106250.021.7480812-13
GTACTTT110900.020.72970214-15
AGTACTT109950.019.6554612-13
GTACCTG22750.019.2142721
TACCTGG22700.019.0447862
CATGGGA73300.018.3373814
AGAGTAC190400.017.98552510-11
TGTCGCG800.001191902217.81224444-45
ACTTTTT130000.017.73883616-17
CATGGGG57400.017.3764974
TAAGGTG11800.017.3077755
GTATAGG8250.017.2776551
AGTACAT95950.017.1313532
AGGACCG4455.456968E-1217.0771275
TACTTTT125850.017.05942714-15
GTATCAA272550.016.8751131
GTGTAAG12850.016.6389881
Produced by FastQC (version 0.11.5)