FastQCFastQC Report
Thu 26 May 2016
SRR936578_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936578_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768747
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT97170.3509529762018704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87440.31581072593487236No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69780.25202736111316776No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT63930.23089867004822037No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46320.16729589232963504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40280.1454809702728346No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38970.14074958817111133No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30740.11102495099768958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG128650.024.5651511
TACATGG131050.023.9697842
ACATGGG128250.023.4185183
GTGTAGC14600.022.459691
GAGTACT88350.022.13235912-13
CGTACAC2858.367351E-1121.676953
GTACCTG25050.021.4377041
GTACTTT95750.020.39447214-15
CATGGGG54050.020.1344384
TACCTGG24350.020.101942
TAGCGCG1456.129414E-419.6645034
GTATCAA242250.019.6371211
AGTACTT96250.019.45180312-13
CATGGGA73850.018.790234
AGAGTAC172800.018.48099110-11
TATCAAC273500.017.30622
ATCAACG271800.017.2920533
TCAACGC271800.017.2570844
CAACGCA271200.017.2261055
AACGCAG274550.016.9986066
Produced by FastQC (version 0.11.5)