##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936578_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2768747 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.178353963002035 31.0 31.0 34.0 28.0 34.0 2 31.22205857017633 31.0 31.0 34.0 28.0 34.0 3 31.255642714917613 31.0 31.0 34.0 27.0 34.0 4 34.899866618365635 37.0 35.0 37.0 32.0 37.0 5 34.82230770814379 37.0 35.0 37.0 32.0 37.0 6 34.938435328327216 37.0 35.0 37.0 32.0 37.0 7 34.775562375327176 37.0 35.0 37.0 32.0 37.0 8 34.75549553642857 37.0 35.0 37.0 32.0 37.0 9 36.23313253251381 39.0 35.0 39.0 32.0 39.0 10-11 36.092850123178465 39.0 35.0 39.0 30.5 39.0 12-13 35.98773813569821 39.0 35.0 39.0 30.0 39.0 14-15 37.01133436894017 39.0 36.0 41.0 31.0 41.0 16-17 36.94452201663785 39.0 36.0 41.0 31.0 41.0 18-19 36.90539637605025 39.0 36.0 41.0 31.0 41.0 20-21 36.80558750944019 39.0 36.0 41.0 30.0 41.0 22-23 36.69453095569945 39.0 36.0 41.0 30.0 41.0 24-25 36.537417467179196 39.0 36.0 40.5 30.0 41.0 26-27 36.33357110635244 39.0 35.5 40.0 29.5 41.0 28-29 36.45428726423903 39.0 36.0 40.5 30.0 41.0 30-31 36.39218227595371 39.0 35.5 40.0 30.0 41.0 32-33 36.239121884375855 39.0 35.0 40.0 30.0 41.0 34-35 36.067650457047904 38.5 35.0 40.0 29.0 41.0 36-37 35.83104415101849 38.0 35.0 40.0 27.5 41.0 38-39 35.64843031884098 38.0 35.0 40.0 27.0 41.0 40-41 35.50632885561592 38.0 34.5 40.0 27.0 41.0 42-43 35.247390245479274 38.0 34.0 40.0 26.0 41.0 44-45 34.979167110609964 38.0 34.0 40.0 25.5 41.0 46-47 34.64137225250266 38.0 33.0 40.0 24.0 41.0 48-49 34.386928274775556 38.0 33.0 40.0 24.0 41.0 50-51 33.68823496693631 37.5 32.0 39.5 23.0 40.5 52-53 33.87622361306396 37.5 33.0 39.5 23.0 40.5 54-55 34.242547621722025 38.0 33.0 40.0 23.0 41.0 56-57 34.16150356099709 38.0 33.0 40.0 23.0 41.0 58-59 33.91957445010324 38.0 33.0 40.0 22.0 41.0 60-61 33.58739386444482 37.0 33.0 40.0 21.5 41.0 62-63 33.27037284374484 37.0 32.0 40.0 20.5 41.0 64-65 32.958951648525485 36.0 32.0 39.0 20.0 41.0 66-67 32.57791051331162 36.0 31.5 39.0 18.5 41.0 68-69 32.191092216081856 35.0 31.0 39.0 18.0 40.0 70-71 31.780607617814123 35.0 31.0 38.0 15.5 40.0 72-73 31.363995879724655 35.0 31.0 37.5 12.5 39.5 74-75 30.936140066246573 35.0 30.0 37.0 9.5 39.0 76-77 30.510836129122666 34.5 30.0 36.5 7.5 39.0 78-79 30.050546149575965 34.0 29.5 36.0 6.5 38.5 80-81 29.622595166694538 34.0 29.0 35.5 2.0 37.0 82-83 29.209620091687682 34.0 29.0 35.0 2.0 37.0 84-85 28.815510951343697 34.0 29.0 35.0 2.0 36.5 86-87 28.47655130642128 34.0 28.5 35.0 2.0 36.0 88-89 28.094408048117074 34.0 27.0 35.0 2.0 36.0 90-91 27.719571705179273 33.5 26.5 35.0 2.0 35.0 92-93 27.33612298270662 33.0 26.0 35.0 2.0 35.0 94-95 26.88935897718354 33.0 25.0 35.0 2.0 35.0 96-97 26.288196068474296 33.0 24.5 35.0 2.0 35.0 98-99 25.605824403602064 33.0 22.0 35.0 2.0 35.0 100-101 23.49856324900758 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.40484507022790694 1101 2 0.4498029975321174 1101 3 0.4664183798313637 1101 4 0.46870524358717347 1101 5 0.3571016781057068 1101 6 0.3435511284047479 1101 7 0.2716329502197894 1101 8 0.31437668797209284 1101 9 0.5934787145366442 1101 10-11 0.5636035973356144 1101 12-13 0.6262552051863253 1101 14-15 0.6828558449476247 1101 16-17 0.7758108986086469 1101 18-19 0.8668566599592893 1101 20-21 0.7659362798222844 1101 22-23 0.7930559462399955 1101 24-25 0.6632769631704463 1101 26-27 0.595418312263071 1101 28-29 0.7165647255305672 1101 30-31 0.6143700593978423 1101 32-33 0.5949816786519477 1101 34-35 0.6257483031760955 1101 36-37 0.7602725754645405 1101 38-39 0.6826380037896911 1101 40-41 0.6478650213840709 1101 42-43 0.38676662739752743 1101 44-45 0.42905738223529255 1101 46-47 0.4336127514129444 1101 48-49 0.5021475813611502 1101 50-51 0.5876308669443091 1101 52-53 0.6908083349309706 1101 54-55 0.6861736836614654 1101 56-57 0.7040821088241813 1101 58-59 0.6558049097344849 1101 60-61 0.4939389638164755 1101 62-63 0.4873001996126831 1101 64-65 0.5530897630822906 1101 66-67 0.5712447609695275 1101 68-69 0.5219760169406769 1101 70-71 0.46675797458204826 1101 72-73 0.5266906358079169 1101 74-75 0.47502059127346286 1101 76-77 0.5579733568173033 1101 78-79 0.5993296414311118 1101 80-81 0.442498305938436 1101 82-83 0.6262754218867492 1101 84-85 0.6238588924851385 1101 86-87 0.5650727162225699 1101 88-89 0.480892861178404 1101 90-91 0.5772803707768901 1101 92-93 0.4000743347925848 1101 94-95 0.4438996877989645 1101 96-97 0.42784719717403874 1101 98-99 0.2535847451187152 1101 100-101 0.49302208891880994 1102 1 0.08126317977269437 1102 2 0.1626112122340544 1102 3 0.25058955935255867 1102 4 0.16654475218295772 1102 5 0.17626141801602557 1102 6 0.16016337438048822 1102 7 0.08291316812957206 1102 8 0.054781377269485176 1102 9 0.3263130026407026 1102 10-11 0.3884697358937075 1102 12-13 0.3881460335454676 1102 14-15 0.2449305553278336 1102 16-17 0.24488862261086553 1102 18-19 0.2161812687180671 1102 20-21 0.08109590269081934 1102 22-23 -0.08534477770626125 1102 24-25 0.08673026059329203 1102 26-27 0.2169634004180665 1102 28-29 0.22737472705680517 1102 30-31 0.0987073097906972 1102 32-33 0.07741139266524044 1102 34-35 -0.046312242905386825 1102 36-37 -0.07245254161037451 1102 38-39 -0.06448654569374668 1102 40-41 -0.1923336417500039 1102 42-43 -0.06485800533035757 1102 44-45 0.014342609900417358 1102 46-47 0.17354325836393514 1102 48-49 0.23745789450603638 1102 50-51 0.29703146314373186 1102 52-53 0.22944563213034286 1102 54-55 0.13256854695452347 1102 56-57 0.37235770919092914 1102 58-59 0.29675971580348914 1102 60-61 -0.3158365331191817 1102 62-63 0.028249836676103257 1102 64-65 0.24015546338620197 1102 66-67 0.19754620085873142 1102 68-69 0.2238263985957829 1102 70-71 0.1460319342228189 1102 72-73 -0.01668063449202606 1102 74-75 -0.007730885690868661 1102 76-77 0.14170405957878174 1102 78-79 0.14588729271653733 1102 80-81 0.18682328325649422 1102 82-83 0.052954301136228565 1102 84-85 0.005467305546389412 1102 86-87 0.09497143735757518 1102 88-89 -0.09602770221669843 1102 90-91 -0.1751683054326243 1102 92-93 -0.1162382108121669 1102 94-95 0.027005878850506093 1102 96-97 -0.2731510632057983 1102 98-99 -0.3843712730668045 1102 100-101 0.06732103871349082 1103 1 0.24844020153241075 1103 2 0.32287908578048174 1103 3 0.20146224887739805 1103 4 0.16036488384435899 1103 5 0.3265841462203056 1103 6 0.11343179201313092 1103 7 0.046314086179265246 1103 8 -0.03527795740483697 1103 9 0.42259877424591963 1103 10-11 0.3950709320974113 1103 12-13 0.33631204645066504 1103 14-15 0.44326378288300106 1103 16-17 0.5812428559415181 1103 18-19 0.3370666044648445 1103 20-21 0.29581827823135143 1103 22-23 0.2011403389127011 1103 24-25 0.2952653011010895 1103 26-27 0.21797395852004797 1103 28-29 0.37989482474474556 1103 30-31 0.1129625060694508 1103 32-33 0.1952505628247181 1103 34-35 0.18383327706830954 1103 36-37 0.4308257300935736 1103 38-39 0.22255646069596935 1103 40-41 0.2218156846657635 1103 42-43 0.3566152638064395 1103 44-45 0.317660749821151 1103 46-47 0.4171019083620564 1103 48-49 0.5407912134299622 1103 50-51 -0.01240076943514623 1103 52-53 0.2614545147454592 1103 54-55 0.5249950987546157 1103 56-57 0.5452924363333622 1103 58-59 0.46957065301704404 1103 60-61 0.5468899656084361 1103 62-63 0.3997747537221201 1103 64-65 0.5453088872486234 1103 66-67 0.4402602793301469 1103 68-69 0.43181769675326365 1103 70-71 0.5288537138622083 1103 72-73 0.32577968444977756 1103 74-75 0.3073983362579895 1103 76-77 0.32281329397344294 1103 78-79 0.42613870220242234 1103 80-81 0.5968071768112999 1103 82-83 0.4746358517406648 1103 84-85 0.19102747560624422 1103 86-87 0.21221955972961837 1103 88-89 0.19732878703804246 1103 90-91 0.3615260751137299 1103 92-93 0.28602934559625126 1103 94-95 0.2454654330456414 1103 96-97 0.44256467739102945 1103 98-99 0.32492728603806853 1103 100-101 0.42346569569454573 1104 1 0.28439535033965413 1104 2 0.3863079678079444 1104 3 0.38108139884150916 1104 4 0.34264408668990853 1104 5 0.3100862250139187 1104 6 0.2573329742350339 1104 7 0.41994003897185195 1104 8 0.46861253315459805 1104 9 0.6357006017094662 1104 10-11 0.500880630639692 1104 12-13 0.5249581089623945 1104 14-15 0.4316299736868885 1104 16-17 0.22203213294074686 1104 18-19 0.5672501946923632 1104 20-21 0.37521712459979994 1104 22-23 0.4727534391030801 1104 24-25 0.5445153158640039 1104 26-27 0.34697024995925574 1104 28-29 0.4019039413066636 1104 30-31 0.4033693797599085 1104 32-33 0.30482305028890977 1104 34-35 0.3135914907538364 1104 36-37 0.5178766378812085 1104 38-39 0.5206060410881719 1104 40-41 0.3431721927524265 1104 42-43 0.4533513249363992 1104 44-45 0.37635419592560737 1104 46-47 0.48195036876071384 1104 48-49 0.34953231012635655 1104 50-51 0.3784814700686212 1104 52-53 0.4255237369260456 1104 54-55 0.6442823628330245 1104 56-57 0.7195460711770281 1104 58-59 0.5292050277837816 1104 60-61 0.44832778567862874 1104 62-63 0.33402677877483455 1104 64-65 0.46661617587926685 1104 66-67 0.3249609020619957 1104 68-69 0.4034023040334489 1104 70-71 0.5093885444960229 1104 72-73 0.17239242896601326 1104 74-75 0.0679510421977092 1104 76-77 0.2426906750657274 1104 78-79 0.2640695380990472 1104 80-81 0.42585536744329033 1104 82-83 0.36450110678046954 1104 84-85 0.36496699657844545 1104 86-87 0.3767748861595557 1104 88-89 0.19565136926746618 1104 90-91 0.19337210581942088 1104 92-93 0.20344833617936686 1104 94-95 0.3948303549210799 1104 96-97 0.5228579711695005 1104 98-99 0.5362740808097968 1104 100-101 0.6881164499868504 1105 1 0.188609902906677 1105 2 0.2529121562240739 1105 3 0.2510153872768015 1105 4 0.29485475172133846 1105 5 0.41452814382601844 1105 6 0.27610581951414304 1105 7 0.42090687951554884 1105 8 8.753108616659233E-4 1105 9 0.3153372903859193 1105 10-11 0.34550618092147545 1105 12-13 0.2284578699964328 1105 14-15 0.2895788773953214 1105 16-17 0.29380409741298763 1105 18-19 0.08647783179518598 1105 20-21 -0.15620341306322416 1105 22-23 -0.15026967755294862 1105 24-25 -0.14458463242678476 1105 26-27 0.003050472507609925 1105 28-29 0.0955865179737927 1105 30-31 -0.06523589268056185 1105 32-33 -0.08371557275096819 1105 34-35 -0.1926763593802434 1105 36-37 -0.1139284406571619 1105 38-39 -0.292188531551389 1105 40-41 -0.3794764400685864 1105 42-43 -0.15643847698737545 1105 44-45 -0.022097731866622894 1105 46-47 -0.01046709749999053 1105 48-49 0.15688688428486586 1105 50-51 0.0946317418312077 1105 52-53 0.33186836470132874 1105 54-55 0.15894754497374208 1105 56-57 0.32832245570366325 1105 58-59 0.13777255813957368 1105 60-61 0.30051065293192636 1105 62-63 0.10383848071975166 1105 64-65 0.15973271027657177 1105 66-67 -0.10569951321960502 1105 68-69 0.06734021975783122 1105 70-71 0.2592933882811117 1105 72-73 0.21201004783266342 1105 74-75 0.061026814431556886 1105 76-77 0.19728432465292656 1105 78-79 0.15499707905181737 1105 80-81 0.21192075956551548 1105 82-83 0.15102069739069535 1105 84-85 -0.10333656465216379 1105 86-87 0.12852515234479256 1105 88-89 0.11037790232086664 1105 90-91 0.015019247291125026 1105 92-93 -0.07200509838891023 1105 94-95 -0.07489187199838199 1105 96-97 -0.06308893181091335 1105 98-99 -0.46760752142209583 1105 100-101 -0.03442152572376855 1106 1 -0.06911606663910064 1106 2 0.01886081403434048 1106 3 -0.06398791618746102 1106 4 0.08990117408752951 1106 5 0.24170000670407177 1106 6 0.2812987334334167 1106 7 0.47278775575494336 1106 8 0.347935407531466 1106 9 0.39200643914733035 1106 10-11 0.4603432320010654 1106 12-13 0.29802397105726186 1106 14-15 0.22156003865594442 1106 16-17 0.1720996177715648 1106 18-19 0.2037457169665302 1106 20-21 0.31689014032519225 1106 22-23 0.25474703162109336 1106 24-25 0.16497395040502738 1106 26-27 0.1723945340060027 1106 28-29 0.04305529094366278 1106 30-31 0.04624958106978738 1106 32-33 -0.01695112114401809 1106 34-35 0.012676361870923358 1106 36-37 0.17020590781695688 1106 38-39 0.13419457677465374 1106 40-41 0.1187210435429975 1106 42-43 0.2730028838240699 1106 44-45 0.04653788343507159 1106 46-47 0.022639339998583807 1106 48-49 -0.0642121500963384 1106 50-51 0.16249275172002342 1106 52-53 0.18102685064751967 1106 54-55 0.2450711365048832 1106 56-57 0.17295381667099008 1106 58-59 0.23212243517396303 1106 60-61 0.22784777895399344 1106 62-63 0.2586308121261638 1106 64-65 0.15298593909812297 1106 66-67 0.17929307646854653 1106 68-69 0.21776745181450252 1106 70-71 0.4080699374020078 1106 72-73 0.5069352132756677 1106 74-75 0.32389084000552515 1106 76-77 0.004239106162639672 1106 78-79 0.2846622480680807 1106 80-81 0.12350782533383153 1106 82-83 0.29957661586767514 1106 84-85 0.3333395927294269 1106 86-87 0.4189855449474287 1106 88-89 0.397017877475939 1106 90-91 0.36409844409965686 1106 92-93 0.2522922709503881 1106 94-95 0.1353045312424932 1106 96-97 0.18517240278864833 1106 98-99 0.12080121603103322 1106 100-101 0.3536557861316538 1107 1 0.3771090031500357 1107 2 0.41212544018365804 1107 3 0.4695273325871163 1107 4 0.41765305674770303 1107 5 0.37219527098883276 1107 6 0.29747926182822226 1107 7 0.5833606919521515 1107 8 0.5025840291540646 1107 9 0.7719886172134451 1107 10-11 0.7204466959882652 1107 12-13 0.6944366684459311 1107 14-15 0.6874162844642271 1107 16-17 0.803254468334778 1107 18-19 0.9162840388285005 1107 20-21 0.9133258207507708 1107 22-23 0.8260139345695094 1107 24-25 0.9014546157572241 1107 26-27 0.8894261403858792 1107 28-29 0.6964776465839861 1107 30-31 0.6115891117588603 1107 32-33 0.6950247211305225 1107 34-35 0.5981418422903175 1107 36-37 0.6790367365368724 1107 38-39 0.7086824649830135 1107 40-41 0.6214060939511157 1107 42-43 0.7658754543087412 1107 44-45 0.7247736867056531 1107 46-47 0.8564234298232805 1107 48-49 0.9175517845458145 1107 50-51 0.7297540876661586 1107 52-53 0.8457698640059093 1107 54-55 0.9186075135449627 1107 56-57 0.9102842495719514 1107 58-59 0.9135644392947881 1107 60-61 0.8756651632693462 1107 62-63 0.9464191921177516 1107 64-65 0.791625229070128 1107 66-67 0.8629660144130114 1107 68-69 0.9728411924179952 1107 70-71 1.08272371340745 1107 72-73 1.1278711806532016 1107 74-75 0.9100738683096061 1107 76-77 1.0316016884345203 1107 78-79 1.069698610995772 1107 80-81 1.2536274312083506 1107 82-83 1.1759441737654406 1107 84-85 1.2160409261487786 1107 86-87 1.2353828329490746 1107 88-89 1.3198849239675283 1107 90-91 1.1797141744427755 1107 92-93 1.2772352501702287 1107 94-95 1.304238756747747 1107 96-97 1.367413437052189 1107 98-99 1.5643153626950692 1107 100-101 1.9011392779941794 1108 1 0.04304653165062078 1108 2 0.0026546316402473735 1108 3 -0.04204785501753605 1108 4 0.014164495430534885 1108 5 0.12551647983696768 1108 6 0.12865444871756182 1108 7 0.18274218822330823 1108 8 0.04429887485300554 1108 9 0.2929735736720005 1108 10-11 0.15748371977884545 1108 12-13 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-2.3980975208272284 2316 84-85 -2.4056671866883654 2316 86-87 -2.5224956603023294 2316 88-89 -2.66845025494413 2316 90-91 -2.7877526805807555 2316 92-93 -3.1005596362723757 2316 94-95 -3.209989549968139 2316 96-97 -3.36976378020967 2316 98-99 -3.78940148822457 2316 100-101 -4.701898554876312 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6763.0 3 2927.0 4 2896.0 5 3144.0 6 3598.0 7 4181.0 8 5280.0 9 6725.0 10 8366.0 11 10450.0 12 12522.0 13 14885.0 14 16297.0 15 19375.0 16 22818.0 17 27102.0 18 24346.0 19 18894.0 20 19576.0 21 21160.0 22 23484.0 23 26809.0 24 30684.0 25 35614.0 26 41727.0 27 48883.0 28 57538.0 29 68720.0 30 82861.0 31 101027.0 32 124424.0 33 156014.0 34 201618.0 35 267933.0 36 365411.0 37 467399.0 38 362894.0 39 54386.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.86318156811735 18.239603926777313 14.196296241443996 22.700918263661343 2 15.415258896134528 19.96054269328265 40.02604962431761 24.598148786265217 3 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22.784138283373213 26.809234614979676 27.949032963766456 22.45759413788066 48-49 22.756779188733034 26.836987775265996 27.79101689546583 22.61521614053514 50-51 22.25748330947446 27.107844208398024 28.05727501763907 22.577397464488445 52-53 22.795260268950752 27.261832848904145 27.378330619988102 22.564576262157004 54-55 22.309482110047508 26.88450115343899 27.710509387696725 23.09550734881678 56-57 22.695412269788637 27.08104245400829 27.4686642020733 22.75488107412977 58-59 23.183329890070837 26.7492736642429 27.33176893637862 22.735627509307644 60-61 22.680891953589576 26.875141197331704 28.113397778007727 22.330569071070993 62-63 22.471270138352644 26.97869269648596 28.01041419109611 22.539622974065292 64-65 22.270932098753168 27.324043522129205 27.704444080449736 22.700580298667894 66-67 22.418156581262274 27.148783658296704 27.368437781159265 23.064621979281757 68-69 22.31923140410196 27.048856435842044 27.669842421081412 22.962069738974584 70-71 22.644399433987992 26.956240625538992 27.433666517066595 22.96569342340642 72-73 22.52366270137744 26.785865532684603 27.867150440259614 22.82332132567834 74-75 22.19067059468778 27.621810522042384 27.425252952291444 22.762265930978387 76-77 22.423438622496576 27.098586645313382 27.389756275687056 23.088218456502982 78-79 22.510621293747466 27.158934441895678 27.432708923982286 22.897735340374574 80-81 22.644763434581023 27.28810678484928 27.267714070665722 22.79941570990398 82-83 22.532463686037 27.189926288506385 27.61271631621682 22.6648937092398 84-85 22.41433864616376 27.32736431549298 27.313439898169346 22.944857140173912 86-87 22.470778418046017 27.353867709187984 27.31429818086724 22.861055691898756 88-89 22.405455757751756 27.29262247534673 26.991188319864424 23.310733447037094 90-91 22.45980873195292 26.97294502258239 27.329285655329926 23.237960590134765 92-93 22.752280090082003 27.014613130161774 27.45602867250416 22.777078107252056 94-95 22.40320035657442 26.88680224946737 27.490815192627664 23.219182201330543 96-97 22.010298926764712 26.8765905835288 27.8281053716665 23.28500511803999 98-99 22.177382501044928 27.112950940544362 27.188850527666503 23.52081603074421 100-101 21.913688643767472 26.880866076409664 27.491971406355237 23.71347387346763 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 210.0 1 166.5 2 188.0 3 264.5 4 242.5 5 262.0 6 456.5 7 921.5 8 1524.0 9 1928.0 10 1931.0 11 1833.5 12 1942.0 13 2120.0 14 2358.0 15 2572.5 16 2721.5 17 3002.5 18 3282.0 19 3563.0 20 3937.5 21 4505.5 22 5437.0 23 6678.5 24 8085.5 25 9988.0 26 12677.0 27 16055.5 28 19646.5 29 24000.5 30 29675.5 31 36037.0 32 42762.5 33 48002.0 34 53413.0 35 58298.5 36 63643.5 37 70070.0 38 73791.5 39 76045.5 40 79126.0 41 84387.5 42 89078.0 43 93204.5 44 97674.0 45 102608.0 46 105213.5 47 112285.5 48 131093.5 49 130158.5 50 110789.5 51 99655.0 52 93690.0 53 91639.0 54 88908.5 55 82799.5 56 76202.5 57 69404.5 58 63542.5 59 56525.5 60 47158.5 61 38515.0 62 31377.0 63 24605.5 64 18449.5 65 14214.5 66 10812.0 67 8361.0 68 6203.5 69 4192.5 70 3120.5 71 2388.5 72 1817.5 73 1486.5 74 1179.0 75 942.5 76 690.5 77 400.5 78 235.5 79 144.0 80 81.5 81 55.0 82 43.5 83 32.0 84 24.5 85 21.0 86 16.0 87 9.5 88 8.5 89 10.5 90 9.0 91 5.5 92 2.5 93 2.0 94 3.0 95 2.0 96 2.5 97 3.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01336344563082145 2 0.006681722815410725 3 0.007476306069135245 4 0.03452825411639272 5 0.04460501447044457 6 0.058510221410623654 7 0.025173842174817707 8 0.022645622731148785 9 0.01372461983705987 10-11 0.04431607510545384 12-13 0.040415393678078934 14-15 0.018925528406893082 16-17 0.021869098187736184 18-19 0.027972942273165442 20-21 0.022121920132103078 22-23 0.013562091444252581 24-25 0.0030519220427146286 26-27 0.0024559846024212397 28-29 0.0015891665074490374 30-31 0.0065011357122915165 32-33 0.001354403273394066 34-35 0.0016433426383848002 36-37 0.0059051982719981275 38-39 0.006483077001979596 40-41 0.0037200943242557015 42-43 7.584658331006769E-4 44-45 0.0011015813290271737 46-47 0.0020045168446232177 48-49 5.778787299814682E-4 50-51 0.0012821684321463825 52-53 8.848768052841231E-4 54-55 0.002528219443668923 56-57 0.0024740433127331605 58-59 0.0019864581343112964 60-61 5.598200196695473E-4 62-63 9.02935515596044E-4 64-65 0.0031241568839623125 66-67 0.0027449239674119737 68-69 0.005995491823557732 70-71 0.009191883548767727 72-73 0.0034853310902007296 74-75 0.0033408614077053626 76-77 0.0031060981736503912 78-79 0.0346004889576404 80-81 0.021724628505240817 82-83 0.0041535033717418025 84-85 0.008270889322859763 86-87 0.007313777676327957 88-89 0.005381495672952422 90-91 0.011864572674932017 92-93 0.01374267854737179 94-95 0.006519194422603437 96-97 0.003936798847998752 98-99 0.022248331104286524 100-101 0.04113774209055576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2768747.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.25929473964793 #Duplication Level Percentage of deduplicated Percentage of total 1 77.64500723909703 43.68253347326457 2 12.940601212813824 14.560581954798765 3 4.098013394220072 6.91654030177356 4 1.7793074374891735 4.004103262326043 5 0.9369208899643575 2.635525424811901 6 0.5674591001868133 1.9154909262063196 7 0.3779747987051709 1.4885216923159312 8 0.26479188500905654 1.191760376271318 9 0.18484063964893263 0.9359103623286917 >10 1.0693098475867666 11.132726844733549 >50 0.08762830262527431 3.384570364884163 >100 0.044021041554884274 4.4735191562844205 >500 0.002642072735083298 1.0378415035523387 >1k 0.001224375169880169 1.4863389793446753 >5k 2.5776319365898294E-4 1.1540353771037541 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9717 0.3509529762018704 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8744 0.31581072593487236 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6978 0.25202736111316776 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6393 0.23089867004822037 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4632 0.16729589232963504 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4028 0.1454809702728346 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3897 0.14074958817111133 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3074 0.11102495099768958 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.611742062384176E-5 0.0 3 0.0 0.0 0.0 7.223484124768352E-5 0.0 4 0.0 0.0 0.0 7.223484124768352E-5 0.0 5 0.0 3.611742062384176E-5 0.0 7.223484124768352E-5 0.0 6 0.0 3.611742062384176E-5 0.0 7.223484124768352E-5 0.0 7 0.0 3.611742062384176E-5 0.0 7.223484124768352E-5 0.0 8 0.0 3.611742062384176E-5 0.0 7.223484124768352E-5 0.0 9 0.0 3.611742062384176E-5 0.0 1.0835226187152527E-4 0.0 10-11 0.0 3.611742062384176E-5 0.0 1.2641097218344616E-4 0.0 12-13 0.0 3.611742062384176E-5 0.0 1.4446968249536704E-4 0.0 14-15 0.0 3.611742062384176E-5 0.0 1.4446968249536704E-4 0.0 16-17 0.0 3.611742062384176E-5 0.0 3.431154959264967E-4 0.0 18-19 0.0 3.611742062384176E-5 0.0 3.9729162686225934E-4 0.0 20-21 0.0 3.611742062384176E-5 0.0 5.056438887337847E-4 0.0 22-23 0.0 3.611742062384176E-5 0.0 5.417613093576264E-4 0.0 24-25 0.0 3.611742062384176E-5 0.0 5.778787299814682E-4 0.0 26-27 0.0 3.611742062384176E-5 0.0 6.862309918529934E-4 0.0 28-29 0.0 3.611742062384176E-5 0.0 0.0013905206940179077 0.0 30-31 0.0 7.223484124768352E-5 0.0 0.0024740433127331605 0.0 32-33 0.0 7.223484124768352E-5 0.0 0.003918740137686831 0.0 34-35 0.0 7.223484124768352E-5 0.0 0.00512867372858553 0.0 36-37 0.0 7.223484124768352E-5 0.0 0.007277660255704114 3.611742062384176E-5 38-39 0.0 7.223484124768352E-5 0.0 0.010726873925281002 3.611742062384176E-5 40-41 0.0 7.223484124768352E-5 0.0 0.014735907614527437 3.611742062384176E-5 42-43 0.0 7.223484124768352E-5 0.0 0.01869076517283811 3.611742062384176E-5 44-45 0.0 7.223484124768352E-5 0.0 0.020767516858709013 3.611742062384176E-5 46-47 0.0 7.223484124768352E-5 0.0 0.0243070240798455 3.611742062384176E-5 48-49 0.0 7.223484124768352E-5 0.0 0.028839760368137644 3.611742062384176E-5 50-51 0.0 7.223484124768352E-5 0.0 0.03481719348138346 3.611742062384176E-5 52-53 0.0 7.223484124768352E-5 0.0 0.04632059195007705 3.611742062384176E-5 54-55 0.0 7.223484124768352E-5 0.0 0.05419418964607456 3.611742062384176E-5 56-57 0.0 7.223484124768352E-5 0.0 0.07313777676327957 3.611742062384176E-5 58-59 0.0 1.4446968249536704E-4 0.0 0.10293464877794901 3.611742062384176E-5 60-61 0.0 1.4446968249536704E-4 0.0 0.12800013869089522 3.611742062384176E-5 62-63 0.0 1.6252839280728792E-4 0.0 0.1417608759485789 3.611742062384176E-5 64-65 0.0 1.805871031192088E-4 0.0 0.16854194334115757 3.611742062384176E-5 66-67 0.0 1.805871031192088E-4 0.0 0.22782868929519381 3.611742062384176E-5 68-69 0.0 2.1670452374305055E-4 0.0 0.31122381351564443 3.611742062384176E-5 70-71 0.0 2.708806546788132E-4 0.0 0.3810749050021544 3.611742062384176E-5 72-73 0.0 3.2505678561457583E-4 0.0 0.45991923422400094 3.611742062384176E-5 74-75 0.0 3.2505678561457583E-4 0.0 0.5540051149491088 3.611742062384176E-5 76-77 0.0 3.2505678561457583E-4 0.0 0.6086688310632933 3.611742062384176E-5 78-79 0.0 3.2505678561457583E-4 0.0 0.6909984913753405 3.611742062384176E-5 80-81 0.0 3.2505678561457583E-4 0.0 0.8076577599903494 3.611742062384176E-5 82-83 0.0 3.2505678561457583E-4 0.0 0.9127052778747932 3.611742062384176E-5 84-85 0.0 3.2505678561457583E-4 0.0 1.05397856864495 3.611742062384176E-5 86-87 0.0 3.2505678561457583E-4 0.0 1.263549901814792 3.611742062384176E-5 88-89 0.0 3.2505678561457583E-4 0.0 1.553897846209856 3.611742062384176E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12865 0.0 24.565151 1 TACATGG 13105 0.0 23.969784 2 ACATGGG 12825 0.0 23.418518 3 GTGTAGC 1460 0.0 22.45969 1 GAGTACT 8835 0.0 22.132359 12-13 CGTACAC 285 8.367351E-11 21.67695 3 GTACCTG 2505 0.0 21.437704 1 GTACTTT 9575 0.0 20.394472 14-15 CATGGGG 5405 0.0 20.134438 4 TACCTGG 2435 0.0 20.10194 2 TAGCGCG 145 6.129414E-4 19.664503 4 GTATCAA 24225 0.0 19.637121 1 AGTACTT 9625 0.0 19.451803 12-13 CATGGGA 7385 0.0 18.79023 4 AGAGTAC 17280 0.0 18.480991 10-11 TATCAAC 27350 0.0 17.3062 2 ATCAACG 27180 0.0 17.292053 3 TCAACGC 27180 0.0 17.257084 4 CAACGCA 27120 0.0 17.226105 5 AACGCAG 27455 0.0 16.998606 6 >>END_MODULE