FastQCFastQC Report
Thu 26 May 2016
SRR936578_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936578_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768747
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT115340.4165783294753909No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96360.3480274651313392No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82440.29775201562295145No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT52350.1890746969658116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49670.17939522823862203No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43060.15552161320626262No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36950.1334538692050953No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT34430.12435227920788719No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29610.10694368246719545No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29200.10546286822161793No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG133600.024.7631471
TACATGG137650.023.6058222
ACATGGG133750.023.1926733
GAGTACT106850.021.2665812-13
GTACTTT111800.020.26127814-15
AGTACTT112750.019.41666412-13
CATGGGA79350.019.156274
TAGCGCG1759.940698E-519.0006244
GTGTAGC14200.018.9361951
TACCTGG24600.018.7316462
GTACCGT2552.68441E-718.6233526
CATGGGG60500.018.608054
GTGTAAG11500.018.371641
AGAGTAC194750.018.3005210-11
GTATCAA271750.017.9168421
GTACCTG26600.017.8709071
ATGGGAG34250.017.7479215
TAAGGTG13750.017.6143075
GTATAGG9300.017.5545221
TACTTTT126550.017.35560814-15
Produced by FastQC (version 0.11.5)