##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936578_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2768747 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.489203961214223 31.0 31.0 34.0 30.0 34.0 2 31.718223803041592 31.0 31.0 34.0 30.0 34.0 3 31.999182662771283 33.0 31.0 34.0 30.0 34.0 4 35.80048195086081 37.0 35.0 37.0 35.0 37.0 5 35.61342116126898 37.0 35.0 37.0 33.0 37.0 6 35.71461820094071 37.0 35.0 37.0 33.0 37.0 7 35.648650815693884 37.0 35.0 37.0 33.0 37.0 8 35.62440916414537 37.0 35.0 37.0 33.0 37.0 9 37.25697725360966 39.0 37.0 39.0 34.0 39.0 10-11 37.14488683870357 39.0 37.0 39.0 33.0 39.0 12-13 37.02838269441014 39.0 37.0 39.0 33.0 39.0 14-15 38.15043880860187 40.0 37.0 41.0 33.0 41.0 16-17 38.06899203863698 40.0 37.0 41.0 33.0 41.0 18-19 38.129883301002224 40.0 37.5 41.0 33.0 41.0 20-21 38.20155073757191 40.0 37.5 41.0 33.0 41.0 22-23 38.122061712391925 40.0 37.0 41.0 33.0 41.0 24-25 38.020973566743365 40.0 37.0 41.0 33.0 41.0 26-27 37.82277542874087 40.0 37.0 41.0 33.0 41.0 28-29 37.74885336218874 40.0 37.0 41.0 32.5 41.0 30-31 37.74760604706751 40.0 37.0 41.0 33.0 41.0 32-33 37.59304479607563 40.0 36.5 41.0 32.5 41.0 34-35 37.431569045492424 39.5 36.0 41.0 32.0 41.0 36-37 37.21681233424361 39.0 36.0 41.0 31.5 41.0 38-39 37.0780715970076 39.0 36.0 41.0 31.0 41.0 40-41 36.865897642507605 39.0 35.5 41.0 31.0 41.0 42-43 36.63352664580765 39.0 35.0 41.0 30.0 41.0 44-45 36.37508663666272 39.0 35.0 40.0 30.0 41.0 46-47 36.011629809440876 39.0 35.0 40.0 29.5 41.0 48-49 35.58642682050762 38.0 35.0 40.0 28.0 41.0 50-51 35.3964658020397 38.0 35.0 40.0 27.5 41.0 52-53 35.13613992177689 38.0 34.0 40.0 27.0 41.0 54-55 34.870258279286624 38.0 34.0 40.0 26.0 41.0 56-57 34.584772642643046 38.0 34.0 40.0 26.0 41.0 58-59 34.2227807380017 37.5 33.0 40.0 25.0 41.0 60-61 33.8391554013422 37.0 33.0 40.0 24.0 41.0 62-63 33.56092430980512 36.5 32.5 39.0 23.5 40.5 64-65 33.12845503760365 36.0 32.0 39.0 22.5 40.0 66-67 32.71775156776694 35.5 31.5 39.0 22.0 40.0 68-69 32.262037123651965 35.0 31.0 38.0 21.5 40.0 70-71 31.781424593868635 35.0 31.0 37.5 20.0 39.5 72-73 31.365605271987654 34.5 30.0 37.0 19.0 39.0 74-75 31.00264993515117 34.0 30.0 36.5 18.0 39.0 76-77 29.567513572023735 32.5 28.5 35.0 16.0 37.0 78-79 30.37481792305328 34.0 30.0 36.0 17.5 37.5 80-81 30.21280817640615 34.0 30.0 35.0 14.0 37.0 82-83 29.873955980810095 34.0 29.5 35.0 10.0 37.0 84-85 29.427419515036945 34.0 29.0 35.0 7.0 36.0 86-87 29.064409279721115 34.0 29.0 35.0 4.5 36.0 88-89 28.649169461854044 33.5 29.0 35.0 2.0 35.0 90-91 28.194024950636518 33.0 27.5 35.0 2.0 35.0 92-93 27.74210373862256 33.0 27.0 35.0 2.0 35.0 94-95 27.212246369928344 33.0 26.0 34.5 2.0 35.0 96-97 26.539217740010194 32.0 25.0 34.0 2.0 35.0 98-99 25.685484986530007 32.0 24.0 34.0 2.0 35.0 100-101 22.910820129105332 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3632344438859185 1101 2 0.397600510262901 1101 3 0.2254373717825935 1101 4 0.10154677232949894 1101 5 0.17870337411779502 1101 6 0.13718041040454665 1101 7 0.17168355946033387 1101 8 0.16104860619832095 1101 9 0.21695800641295193 1101 10-11 0.2261401138000636 1101 12-13 0.16507363384830853 1101 14-15 0.30035674999858486 1101 16-17 0.14729835003752356 1101 18-19 0.23904622413911625 1101 20-21 0.12470642159685497 1101 22-23 0.03609566182504409 1101 24-25 6.88422943220246E-4 1101 26-27 0.03704730768141218 1101 28-29 0.04620028992432168 1101 30-31 -0.0718725261357136 1101 32-33 0.08633636501402009 1101 34-35 0.1787547932462772 1101 36-37 0.1431732297111381 1101 38-39 0.18843410384040737 1101 40-41 0.29806559341312777 1101 42-43 0.41691446156951173 1101 44-45 0.43670522563803615 1101 46-47 0.36113884057992607 1101 48-49 0.3877586690013999 1101 50-51 0.35708703695508603 1101 52-53 0.33210845863812466 1101 54-55 0.31069029305754015 1101 56-57 0.3636751347227474 1101 58-59 0.28713112994747547 1101 60-61 0.35915386423586426 1101 62-63 0.21021526442441285 1101 64-65 0.2820163835597569 1101 66-67 0.0400660321270081 1101 68-69 -0.06852500580855292 1101 70-71 0.0040438517397092255 1101 72-73 0.04025413781366893 1101 74-75 0.06776854665116616 1101 76-77 0.532062672977009 1101 78-79 0.33170074436881336 1101 80-81 0.2719079477463353 1101 82-83 0.31299573873111086 1101 84-85 0.38331861714704374 1101 86-87 0.29415207143419764 1101 88-89 0.277109869385356 1101 90-91 0.1880279944826313 1101 92-93 0.10994702610200235 1101 94-95 -0.15366266003614015 1101 96-97 0.055343503907455016 1101 98-99 0.23466614183340795 1101 100-101 0.07949382120173354 1102 1 0.3579724573748848 1102 2 0.4533708180063236 1102 3 0.14976996089466965 1102 4 0.07374333360520069 1102 5 0.07807598733966614 1102 6 0.07688217611442383 1102 7 0.09030383666380715 1102 8 -0.029246855995907595 1102 9 0.043751867999624494 1102 10-11 0.07542149458316771 1102 12-13 0.18531176901790047 1102 14-15 0.28143742492186163 1102 16-17 0.2662182562450468 1102 18-19 0.1754979393568803 1102 20-21 0.18025370764972592 1102 22-23 0.07199914639749494 1102 24-25 0.17493437768658282 1102 26-27 -0.027686636872388704 1102 28-29 -0.006371504453660748 1102 30-31 0.18271894247665443 1102 32-33 0.2381236022192894 1102 34-35 0.32858137667734155 1102 36-37 0.31057331064125293 1102 38-39 0.25059486236087736 1102 40-41 0.3494574883512058 1102 42-43 0.31960502583019235 1102 44-45 0.10656787384296251 1102 46-47 0.2563322567145434 1102 48-49 0.32239934613909327 1102 50-51 0.40644655434328314 1102 52-53 0.1950639492180457 1102 54-55 0.33071009636412896 1102 56-57 0.42151197419494224 1102 58-59 0.39356783669754947 1102 60-61 0.5050922237933193 1102 62-63 0.32168680690767104 1102 64-65 0.18384605860671144 1102 66-67 0.23733893818781837 1102 68-69 0.20956948259395602 1102 70-71 0.14948004291461814 1102 72-73 0.08753111688776016 1102 74-75 -0.25139348719654464 1102 76-77 0.06318859963850443 1102 78-79 0.21665591012719787 1102 80-81 0.10932778610429139 1102 82-83 0.20764727587816623 1102 84-85 0.3680151491113257 1102 86-87 0.2456652697813766 1102 88-89 0.21827256735342715 1102 90-91 0.3597215615583309 1102 92-93 0.46700235678938995 1102 94-95 0.24483239469363838 1102 96-97 0.3957009584851967 1102 98-99 0.10147524332056435 1102 100-101 -0.14264704837181696 1103 1 0.39568643465294784 1103 2 0.38185226729951083 1103 3 0.23684841443228422 1103 4 0.09667374774932114 1103 5 0.016765095221010995 1103 6 -0.011236898793612227 1103 7 0.06471331449234441 1103 8 0.08106087594269695 1103 9 0.04991090819146393 1103 10-11 0.04077858824714298 1103 12-13 0.0964452878527382 1103 14-15 0.051188795797081355 1103 16-17 0.12152752769809183 1103 18-19 0.11408192404466178 1103 20-21 -0.010621373182154059 1103 22-23 -0.005054729926932566 1103 24-25 -0.04242403561732999 1103 26-27 -0.05391610174424244 1103 28-29 0.01196345419558753 1103 30-31 0.014370779568317005 1103 32-33 -0.14913069196492756 1103 34-35 -0.04566343107199344 1103 36-37 -0.008401504395969539 1103 38-39 0.02848900703829571 1103 40-41 0.09281463585397631 1103 42-43 0.13506538583882133 1103 44-45 0.2452806786535504 1103 46-47 0.29459592022443815 1103 48-49 0.5341224194225376 1103 50-51 0.39537231712994725 1103 52-53 0.4183211000022311 1103 54-55 0.41978554671876367 1103 56-57 0.2807506425532793 1103 58-59 0.26213884112706864 1103 60-61 0.24099569476467764 1103 62-63 0.05413698097006403 1103 64-65 0.057658485818713245 1103 66-67 0.023881774317267457 1103 68-69 0.05864376971624807 1103 70-71 -0.07918648214684865 1103 72-73 -0.07315361433188627 1103 74-75 -0.012513801772296773 1103 76-77 -0.19405301295521227 1103 78-79 -0.04342584624361123 1103 80-81 0.0896984858533898 1103 82-83 0.09626168734449436 1103 84-85 0.24176671294163654 1103 86-87 0.250944713756315 1103 88-89 0.14580471491023417 1103 90-91 0.05191765777415114 1103 92-93 0.23419682703431732 1103 94-95 0.2575648274781628 1103 96-97 -0.12821108032117579 1103 98-99 -0.47355319397698636 1103 100-101 -0.19826031252164356 1104 1 0.27045259107824293 1104 2 0.3616873943024679 1104 3 0.13904377172499593 1104 4 0.10249296666549412 1104 5 0.03899414508769894 1104 6 0.06210554759362452 1104 7 -0.028417380070095533 1104 8 -0.013925853026385937 1104 9 0.0440102252776029 1104 10-11 -0.08973716266088871 1104 12-13 -0.050409207897786246 1104 14-15 0.16427363860177024 1104 16-17 0.09787261425943683 1104 18-19 -0.009875390379669113 1104 20-21 -0.0439011215246623 1104 22-23 -0.024417192291245726 1104 24-25 -0.0384465266967311 1104 26-27 0.15628629926091975 1104 28-29 -0.012853734162227681 1104 30-31 -0.0050449851963989545 1104 32-33 0.011442155850772906 1104 34-35 0.10646663808860524 1104 36-37 0.13160022232896296 1104 38-39 0.07501030919157614 1104 40-41 0.11453463318008517 1104 42-43 0.0829160518069969 1104 44-45 0.2733475594263126 1104 46-47 0.3510068152740544 1104 48-49 0.315249462137146 1104 50-51 0.29569619498774813 1104 52-53 0.24911896385250287 1104 54-55 0.4110018081020428 1104 56-57 0.4248660088265197 1104 58-59 0.4016073976805288 1104 60-61 0.2677968140566165 1104 62-63 0.12735196933221005 1104 64-65 0.20489077573076742 1104 66-67 0.15656392187195678 1104 68-69 0.2853119350866038 1104 70-71 0.1432536919990106 1104 72-73 0.23524530434073654 1104 74-75 0.0978273316413727 1104 76-77 0.34493613381854615 1104 78-79 0.36116675483752303 1104 80-81 0.3338789746022961 1104 82-83 0.3944091024898988 1104 84-85 0.5723826957492264 1104 86-87 0.6162016050161085 1104 88-89 0.5022587683385353 1104 90-91 0.32389763828905416 1104 92-93 0.41037090741199833 1104 94-95 0.46663345621885455 1104 96-97 0.16877503435479824 1104 98-99 0.21063753818751252 1104 100-101 0.21963494036852538 1105 1 0.3259659703535611 1105 2 0.4363290269487514 1105 3 0.1737414508626891 1105 4 0.09207606822706538 1105 5 -0.09361282666625925 1105 6 0.007758369661445386 1105 7 0.04529479218238208 1105 8 -0.01652496011547555 1105 9 0.01025832326580911 1105 10-11 0.060095976503326654 1105 12-13 -0.013505803248463621 1105 14-15 0.12309035512347322 1105 16-17 0.131429012153923 1105 18-19 0.014917142371714931 1105 20-21 0.03176090021337785 1105 22-23 0.10861979952041878 1105 24-25 -0.008091768307487257 1105 26-27 -0.023932451749608674 1105 28-29 0.05331150449371336 1105 30-31 0.05289780715420278 1105 32-33 0.06801107935882555 1105 34-35 -0.04079483576323639 1105 36-37 0.1393586801070441 1105 38-39 0.05056680617312992 1105 40-41 0.0038451859820725076 1105 42-43 -0.054196118012804106 1105 44-45 -0.20897340968077316 1105 46-47 -0.05346643166366505 1105 48-49 -0.019405081494234366 1105 50-51 -0.036176049153290535 1105 52-53 -0.0355227192273162 1105 54-55 -0.08783777557801642 1105 56-57 -0.10848865492568649 1105 58-59 -0.06861219290002651 1105 60-61 -0.11912096415034057 1105 62-63 -0.1715920711000578 1105 64-65 -0.04451077103610146 1105 66-67 -0.12366987019888853 1105 68-69 -0.24087879327154837 1105 70-71 -0.15138169282221625 1105 72-73 -0.24807662211352266 1105 74-75 -0.2333050228660376 1105 76-77 -0.3143804383742399 1105 78-79 -0.17323855812778177 1105 80-81 -0.2198361524620296 1105 82-83 -0.1063804790096583 1105 84-85 0.03312716000119664 1105 86-87 -0.03693033237629706 1105 88-89 -0.029464667205775896 1105 90-91 -0.20321587543613617 1105 92-93 0.04402511669871245 1105 94-95 0.021202245039699363 1105 96-97 -0.18202669569347307 1105 98-99 -0.3301897438632224 1105 100-101 -0.052484540830594284 1106 1 0.2102161199387389 1106 2 0.2901609255833577 1106 3 0.06322468353329569 1106 4 -0.02036042552033024 1106 5 -0.08518539556236959 1106 6 -0.07792845713031227 1106 7 -0.014256296402017199 1106 8 -0.026370902370196347 1106 9 -0.0022139030532670745 1106 10-11 -0.09507991048581488 1106 12-13 0.03774180908430935 1106 14-15 0.013924350948279596 1106 16-17 0.06108980452969348 1106 18-19 -0.035235110758783605 1106 20-21 -0.09762593245386597 1106 22-23 -0.07780949735129639 1106 24-25 -0.06422787822950937 1106 26-27 -0.1077457166874396 1106 28-29 -0.05531731202672319 1106 30-31 -0.08604928705628367 1106 32-33 -0.019917126950012687 1106 34-35 -0.22637173693389911 1106 36-37 -0.10801623490818457 1106 38-39 0.053986131499009105 1106 40-41 0.036326003706378174 1106 42-43 -0.12719218623083606 1106 44-45 -0.05324399757750342 1106 46-47 -0.1550626464092275 1106 48-49 -0.2602989713443904 1106 50-51 -0.25315444653545427 1106 52-53 -0.19818176214596406 1106 54-55 -0.3451253316499745 1106 56-57 -0.45945190553451454 1106 58-59 -0.5232568576553618 1106 60-61 -0.2394950649905283 1106 62-63 -0.5858380958514644 1106 64-65 -0.28831662566209104 1106 66-67 -0.31514789166960355 1106 68-69 -0.2367391208840708 1106 70-71 -0.3408071898107288 1106 72-73 -0.24396108120272686 1106 74-75 -0.24127425568292793 1106 76-77 -0.33583706826513193 1106 78-79 -0.21654721440322433 1106 80-81 -0.2718528244645988 1106 82-83 -0.0904367674026858 1106 84-85 0.0391775657097746 1106 86-87 -0.034484659674660634 1106 88-89 -0.22889063783072316 1106 90-91 -0.3064491335871793 1106 92-93 0.09013392357854855 1106 94-95 0.10897681927651703 1106 96-97 -0.07812584998264072 1106 98-99 -0.0014767639466057858 1106 100-101 -0.0257600545462644 1107 1 0.6096744057267536 1107 2 0.5708681445418087 1107 3 0.33682631358166404 1107 4 0.1627122317891434 1107 5 0.03760349516336703 1107 6 0.1329086495875913 1107 7 0.10336981125267641 1107 8 0.1816077731348571 1107 9 0.22055825440876475 1107 10-11 0.2815663671358237 1107 12-13 0.36931999777907265 1107 14-15 0.45223976154493783 1107 16-17 0.36816132209558816 1107 18-19 0.3145651989073954 1107 20-21 0.21554936225989962 1107 22-23 0.30209607059180854 1107 24-25 0.36261348639838786 1107 26-27 0.28142833210380047 1107 28-29 0.37480955164891583 1107 30-31 0.3668998330697306 1107 32-33 0.43883739405526967 1107 34-35 0.3385901192890586 1107 36-37 0.43879053420099723 1107 38-39 0.38299653703537473 1107 40-41 0.4519596097448755 1107 42-43 0.4778775894627856 1107 44-45 0.42082162772911147 1107 46-47 0.5930735099399342 1107 48-49 0.3701129626311257 1107 50-51 0.33180869996325413 1107 52-53 0.15951997448456012 1107 54-55 0.4143929721212558 1107 56-57 0.30420401689840304 1107 58-59 0.17915195949777285 1107 60-61 0.13017733937113718 1107 62-63 0.15261494415918975 1107 64-65 0.14761043074849312 1107 66-67 0.26616904978547495 1107 68-69 0.3102267486341006 1107 70-71 0.1912296179932902 1107 72-73 0.3512663097387332 1107 74-75 0.6561822546318865 1107 76-77 0.4683518254174892 1107 78-79 0.6527945567959392 1107 80-81 0.4888406718735716 1107 82-83 0.537012404468765 1107 84-85 0.6172444570994635 1107 86-87 0.715838413278334 1107 88-89 0.8623152256661655 1107 90-91 0.6900909785324103 1107 92-93 0.7277445210434266 1107 94-95 0.8772918468225583 1107 96-97 0.8738490111447099 1107 98-99 1.0716250858518563 1107 100-101 0.7230575286866028 1108 1 0.2786102705381488 1108 2 0.3200582062789259 1108 3 0.0595215735278849 1108 4 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-1.152553435494852 2316 74-75 -1.3300857358483391 2316 76-77 -1.2574759059094553 2316 78-79 -1.217414541522185 2316 80-81 -1.1481068682073712 2316 82-83 -0.9713457749921162 2316 84-85 -0.9751575759914957 2316 86-87 -1.0438189016764454 2316 88-89 -1.37624074257144 2316 90-91 -1.5237757480432492 2316 92-93 -1.5490708878236958 2316 94-95 -1.7677534906181052 2316 96-97 -1.8522589572980088 2316 98-99 -2.2403908406839648 2316 100-101 -2.64870581079553 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 17.0 4 31.0 5 84.0 6 194.0 7 468.0 8 1054.0 9 2027.0 10 3352.0 11 5129.0 12 7505.0 13 9949.0 14 12996.0 15 17035.0 16 21621.0 17 27046.0 18 23890.0 19 16563.0 20 15788.0 21 16129.0 22 17552.0 23 20015.0 24 23442.0 25 28217.0 26 34933.0 27 43766.0 28 54741.0 29 68340.0 30 84376.0 31 104022.0 32 129417.0 33 164326.0 34 214454.0 35 288342.0 36 406627.0 37 537802.0 38 345451.0 39 22046.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.28062613421845 19.312461462583705 14.933880836924073 22.473031566273768 2 15.59397008449294 20.92492514621277 39.24119264686387 24.239912122430418 3 20.103877903732347 24.43182290189931 29.70713004783946 25.757169146528884 4 13.247959728086562 16.90139471409463 36.84810725113342 33.00253830668539 5 13.63071454343788 37.85340444612672 33.74497561532346 14.77090539511194 6 32.03795796437883 36.19218368453311 16.97547663257062 14.794381718517439 7 28.917160000534537 32.910988255698335 20.092626736931905 18.079225006835223 8 26.870855300249534 34.20709801220552 19.23332106544946 19.688725622095482 9 26.370900805951198 17.058036143194492 20.347324006538916 36.223739044315394 10-11 25.331599691194995 26.61301405423952 26.693014415414968 21.362371839150516 12-13 26.67604396144173 23.97003407139951 27.314239360114307 22.039682607044455 14-15 23.013852302423608 25.42557631244908 26.345451396670445 25.21511998845687 16-17 22.08219096941685 28.791037967716083 26.78977169094901 22.336999371918058 18-19 21.96051137933513 27.37848564711763 29.19412644058847 21.46687653295877 20-21 23.004642355174933 26.020394434952582 29.23323938585182 21.74172382402066 22-23 22.437455021882954 26.146189151436182 29.255690606537527 22.160665220143333 24-25 22.703844013194416 26.239793668399457 29.35578801530078 21.70057430310534 26-27 22.404500956209404 26.27652600727044 29.527529620820985 21.79144341569917 28-29 21.846416447584414 27.038006722896675 29.3540002029799 21.761576626539007 30-31 22.201151712724823 26.265771580347202 29.297848195536897 22.235228511391078 32-33 22.033441008514387 26.469132342230456 29.323376434106457 22.1740502151487 34-35 22.804565984104656 26.35886063491089 29.026613236730853 21.809960144253605 36-37 22.742261770823664 25.99593164851668 29.277105518171236 21.98470106248842 38-39 22.306187320383295 26.477430371832995 29.352308971977536 21.864073335806175 40-41 22.524186247514827 26.486701830355035 29.031081885692068 21.958030036438068 42-43 22.335875681573096 26.5796665960509 28.589958437834735 22.49449928454127 44-45 22.564810293365543 26.454005784672642 28.52353598950553 22.45764793245629 46-47 22.981728201718283 26.892129246543405 28.05981017035836 22.06633238137995 48-49 23.030633259292316 26.932568975266662 27.805384881148182 22.231412884292837 50-51 22.516475445613107 27.136715363279674 28.101828935637364 22.24498025546986 52-53 23.03435666956995 27.351012955332376 27.40235463829577 22.21227573680191 54-55 22.55322146178304 26.966726596779306 27.73512034032839 22.744931601109265 56-57 22.957410447732894 27.165691130528124 27.49791098436656 22.378987437372423 58-59 23.473125392442245 26.838580072845946 27.304806427664484 22.38348810704732 60-61 22.904832001710602 26.961518116733117 28.11447747600831 22.019172405547973 62-63 22.70457724693382 27.09353903476503 27.96944601258437 22.23243770571678 64-65 22.506628202412 27.450881583883447 27.642823560945416 22.399666652759137 66-67 22.69536936557204 27.255410063967776 27.284052083688877 22.765168486771305 68-69 22.641510115424264 27.115942908367774 27.603005263762388 22.63954171244558 70-71 22.94573061045817 27.06540271111279 27.367058840101123 22.621807838327918 72-73 22.880859623660022 26.839257404027418 27.7384368702588 22.541446102053765 74-75 22.52240815069055 27.7286801574864 27.28409276831722 22.46481892350583 76-77 22.71820471845429 27.213650184408017 27.278228657275218 22.78991643986247 78-79 22.803714079722823 27.25646308531865 27.288122920347973 22.651699914610553 80-81 22.985001410599253 27.429779791187965 27.168358362288846 22.416860435923937 82-83 22.841114797692644 27.346038765033914 27.41889545714865 22.39395098012479 84-85 22.70050766921856 27.451775036255636 27.241916753806617 22.60580054071919 86-87 22.69702735333399 27.442353555229065 27.093896768728833 22.766722322708116 88-89 22.711833912609787 27.441898189084434 26.78988476911463 23.056383129191143 90-91 22.79045580008457 27.15895915282284 27.07016211961192 22.980422927480674 92-93 23.013293709127606 27.235380929093232 27.18183551861213 22.56948984316703 94-95 22.669857508365844 27.140340868148055 27.21787246999646 22.971929153489633 96-97 22.425252499529975 27.058063288014083 27.545570740233643 22.9711134722223 98-99 22.423923359427416 27.356960383889152 26.840526890391303 23.378589366292125 100-101 22.16821696004034 27.37554473082364 27.09690551172143 23.359332797414588 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 345.0 1 273.5 2 256.5 3 311.5 4 269.0 5 264.0 6 448.5 7 927.5 8 1555.0 9 1985.0 10 1977.5 11 1894.5 12 2050.5 13 2308.5 14 2630.0 15 2875.5 16 3080.5 17 3382.0 18 3726.0 19 4148.5 20 4547.0 21 5076.5 22 5905.5 23 7001.0 24 8404.5 25 10377.5 26 13175.5 27 16662.0 28 20199.0 29 24486.5 30 30391.5 31 36588.5 32 43211.0 33 48708.5 34 53549.5 35 58317.5 36 63968.5 37 70386.0 38 73962.0 39 76103.0 40 79047.5 41 84133.5 42 88892.5 43 92713.0 44 97509.0 45 102396.5 46 104251.5 47 112091.0 48 132016.0 49 130140.0 50 109529.5 51 98772.0 52 93467.0 53 90622.5 54 87946.0 55 82642.5 56 75747.0 57 68738.0 58 63109.5 59 56526.5 60 46879.0 61 38079.0 62 31026.5 63 24055.0 64 17995.0 65 13860.5 66 10440.5 67 8147.5 68 6068.0 69 4048.0 70 2885.5 71 2176.0 72 1762.5 73 1537.0 74 1204.0 75 935.5 76 694.0 77 406.0 78 244.5 79 158.5 80 106.5 81 75.0 82 47.5 83 28.5 84 15.0 85 9.0 86 7.5 87 3.0 88 0.5 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0664029613395518 2 0.012641097218344617 3 0.004045151109870277 4 0.013218975948326085 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0033589201180172835 10-11 3.431154959264967E-4 12-13 9.02935515596044E-5 14-15 5.4176130935762644E-5 16-17 0.0 18-19 0.0 20-21 5.4176130935762644E-5 22-23 0.0014266381146417495 24-25 0.0 26-27 7.223484124768352E-5 28-29 0.0 30-31 3.611742062384176E-5 32-33 0.0029074523602192616 34-35 4.3340904748610115E-4 36-37 0.002094810396182822 38-39 1.4446968249536704E-4 40-41 0.0027629826777238946 42-43 0.00879459192190547 44-45 0.0018419884518159296 46-47 0.0019503407136874551 48-49 0.008541769977538576 50-51 1.9864581343112967E-4 52-53 0.001498872955889433 54-55 0.00883070934252931 56-57 0.0035575659314484136 58-59 9.932290671556482E-4 60-61 0.004785558232659033 62-63 0.00267268912616429 64-65 0.016704307038526815 66-67 0.0031602743045861543 68-69 1.805871031192088E-4 70-71 0.0011376987496510154 72-73 1.805871031192088E-5 74-75 0.0 76-77 8.126419640364396E-4 78-79 0.009209942259079649 80-81 0.015169316662013538 82-83 0.010781050056216766 84-85 0.007656893172254453 86-87 0.009191883548767727 88-89 0.028261881638156173 90-91 0.02298873822707528 92-93 0.0024921020230450814 94-95 0.007169307993832589 96-97 0.009986466802492247 98-99 0.0086501222394101 100-101 0.010564345532473715 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2768747.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.90787497365266 #Duplication Level Percentage of deduplicated Percentage of total 1 75.82467619401433 40.87547164184622 2 13.857177419077047 14.940219755906565 3 4.480404997671107 7.245873373373477 4 1.9611396542523152 4.228834851492248 5 1.0259158058977762 2.7652470498915718 6 0.6367802155628318 2.0596480947754037 7 0.4074905528200888 1.5376864842058966 8 0.29373263452707904 1.266760171021391 9 0.19821418529604728 0.9616774967049366 >10 1.163140126553397 11.590873927660216 >50 0.09862582302084232 3.6527282541318784 >100 0.04778280910397897 4.686568653408197 >500 0.0030326191664101767 1.1061922419057049 >1k 0.0016173968887166507 1.823442510584777 >5k 2.0217461108958134E-4 0.8397528218076014 >10k+ 6.739153702986043E-5 0.419022671283966 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11534 0.4165783294753909 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9636 0.3480274651313392 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8244 0.29775201562295145 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5235 0.1890746969658116 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4967 0.17939522823862203 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4306 0.15552161320626262 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3695 0.1334538692050953 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3443 0.12435227920788719 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2961 0.10694368246719545 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2920 0.10546286822161793 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.611742062384176E-5 2 0.0 0.0 0.0 7.223484124768352E-5 3.611742062384176E-5 3 0.0 0.0 0.0 7.223484124768352E-5 3.611742062384176E-5 4 0.0 0.0 0.0 1.0835226187152527E-4 3.611742062384176E-5 5 0.0 3.611742062384176E-5 0.0 1.0835226187152527E-4 3.611742062384176E-5 6 0.0 3.611742062384176E-5 0.0 1.0835226187152527E-4 3.611742062384176E-5 7 0.0 3.611742062384176E-5 0.0 1.4446968249536704E-4 3.611742062384176E-5 8 0.0 3.611742062384176E-5 0.0 1.4446968249536704E-4 3.611742062384176E-5 9 0.0 3.611742062384176E-5 0.0 1.805871031192088E-4 3.611742062384176E-5 10-11 0.0 3.611742062384176E-5 0.0 1.805871031192088E-4 3.611742062384176E-5 12-13 0.0 3.611742062384176E-5 0.0 1.805871031192088E-4 3.611742062384176E-5 14-15 0.0 3.611742062384176E-5 0.0 1.805871031192088E-4 3.611742062384176E-5 16-17 0.0 3.611742062384176E-5 0.0 2.1670452374305055E-4 3.611742062384176E-5 18-19 0.0 3.611742062384176E-5 0.0 2.5282194436689233E-4 3.611742062384176E-5 20-21 0.0 3.611742062384176E-5 0.0 2.889393649907341E-4 7.223484124768352E-5 22-23 0.0 3.611742062384176E-5 0.0 2.889393649907341E-4 7.223484124768352E-5 24-25 0.0 3.611742062384176E-5 0.0 3.2505678561457583E-4 7.223484124768352E-5 26-27 0.0 3.611742062384176E-5 0.0 4.875851784218638E-4 7.223484124768352E-5 28-29 0.0 3.611742062384176E-5 0.0 0.0011376987496510154 7.223484124768352E-5 30-31 0.0 3.611742062384176E-5 0.0 0.002112869106494743 7.223484124768352E-5 32-33 0.0 3.611742062384176E-5 0.0 0.0035395072211364923 7.223484124768352E-5 34-35 0.0 3.611742062384176E-5 0.0 0.004821675653282875 7.223484124768352E-5 36-37 0.0 3.611742062384176E-5 0.0 0.00698872089071338 7.223484124768352E-5 38-39 0.0 3.611742062384176E-5 0.0 0.01045599327060219 7.223484124768352E-5 40-41 0.0 3.611742062384176E-5 0.0 0.014681731483591674 7.223484124768352E-5 42-43 0.0 3.611742062384176E-5 0.0 0.018654647752214266 7.223484124768352E-5 44-45 0.0 3.611742062384176E-5 0.0 0.020821692989644775 7.223484124768352E-5 46-47 0.0 3.611742062384176E-5 0.0 0.02446955247265279 7.223484124768352E-5 48-49 0.0 3.611742062384176E-5 0.0 0.02896617134032109 7.223484124768352E-5 50-51 0.0 3.611742062384176E-5 0.0 0.03469078250920001 7.223484124768352E-5 52-53 0.0 3.611742062384176E-5 0.0 0.04612194613664593 7.223484124768352E-5 54-55 0.0 3.611742062384176E-5 0.0 0.0538871915707719 7.223484124768352E-5 56-57 0.0 3.611742062384176E-5 0.0 0.07268630900548154 7.223484124768352E-5 58-59 0.0 3.611742062384176E-5 0.0 0.10237482875827947 7.223484124768352E-5 60-61 0.0 3.611742062384176E-5 0.0 0.1278376102980879 7.223484124768352E-5 62-63 0.0 3.611742062384176E-5 0.0 0.1416705823970193 7.223484124768352E-5 64-65 0.0 3.611742062384176E-5 0.0 0.1680362994524238 7.223484124768352E-5 66-67 0.0 3.611742062384176E-5 0.0 0.22725081056521235 7.223484124768352E-5 68-69 0.0 7.223484124768352E-5 0.0 0.3100138799247457 7.223484124768352E-5 70-71 0.0 1.0835226187152527E-4 0.0 0.3797566191493842 7.223484124768352E-5 72-73 0.0 1.0835226187152527E-4 0.0 0.45818559803405656 7.223484124768352E-5 74-75 0.0 1.0835226187152527E-4 0.0 0.5524340071519716 7.223484124768352E-5 76-77 0.0 1.4446968249536704E-4 0.0 0.6070074297145965 7.223484124768352E-5 78-79 0.0 1.4446968249536704E-4 0.0 0.6894815597091392 7.223484124768352E-5 80-81 0.0 1.4446968249536704E-4 0.0 0.8067909418953771 7.223484124768352E-5 82-83 0.0 1.4446968249536704E-4 0.0 0.9114050507323349 7.223484124768352E-5 84-85 0.0 1.4446968249536704E-4 0.0 1.0526061066612442 7.223484124768352E-5 86-87 0.0 1.4446968249536704E-4 0.0 1.2625566727476363 7.223484124768352E-5 88-89 0.0 1.4446968249536704E-4 0.0 1.5546021359120208 7.223484124768352E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13360 0.0 24.763147 1 TACATGG 13765 0.0 23.605822 2 ACATGGG 13375 0.0 23.192673 3 GAGTACT 10685 0.0 21.26658 12-13 GTACTTT 11180 0.0 20.261278 14-15 AGTACTT 11275 0.0 19.416664 12-13 CATGGGA 7935 0.0 19.15627 4 TAGCGCG 175 9.940698E-5 19.000624 4 GTGTAGC 1420 0.0 18.936195 1 TACCTGG 2460 0.0 18.731646 2 GTACCGT 255 2.68441E-7 18.623352 6 CATGGGG 6050 0.0 18.60805 4 GTGTAAG 1150 0.0 18.37164 1 AGAGTAC 19475 0.0 18.30052 10-11 GTATCAA 27175 0.0 17.916842 1 GTACCTG 2660 0.0 17.870907 1 ATGGGAG 3425 0.0 17.747921 5 TAAGGTG 1375 0.0 17.614307 5 GTATAGG 930 0.0 17.554522 1 TACTTTT 12655 0.0 17.355608 14-15 >>END_MODULE