FastQCFastQC Report
Thu 26 May 2016
SRR936577_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936577_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784436
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT101160.36330517203483936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85020.30534011196522387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69160.24838064153746034No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT63090.22658089465873876No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT47010.16883131808380583No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40240.14451759710045411No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC39590.14218319257472609No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30280.10874733698314487No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG127200.025.5531731
TACCTGG24550.025.1646632
GTACCTG25950.024.9044741
TACATGG130850.024.369582
ACATGGG129100.023.6692223
TAGCGCG1903.2725984E-722.512684
GAGTACT92750.022.46791312-13
CATGGGG57750.022.138014
GTACTTT97400.021.05334514-15
AGTACTT97950.019.86810912-13
GTGTAGC15850.018.588241
GTATCAA249900.018.5211751
TAGCCCT18600.018.3974594
GTAGCGC3105.5588316E-918.3958053
AGAGTAC176650.018.31981310-11
ACTTTTT118250.017.96217216-17
CGCGCGT2704.8572474E-717.6002947
CATGGGA75450.017.3225464
TAGCGCC2202.9055102E-517.2824614
TACTTTT113550.017.0963814-15
Produced by FastQC (version 0.11.5)