##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936577_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2784436 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.195414439405322 31.0 31.0 34.0 28.0 34.0 2 31.226960863887697 31.0 31.0 34.0 28.0 34.0 3 31.271459642096282 31.0 31.0 34.0 27.0 34.0 4 34.90291283405329 37.0 35.0 37.0 32.0 37.0 5 34.87901894674541 37.0 35.0 37.0 32.0 37.0 6 34.99424228102208 37.0 35.0 37.0 32.0 37.0 7 34.85423223949123 37.0 35.0 37.0 32.0 37.0 8 34.798341208057934 37.0 35.0 37.0 32.0 37.0 9 36.24784803816644 39.0 35.0 39.0 32.0 39.0 10-11 36.13921706227042 39.0 35.0 39.0 31.0 39.0 12-13 36.02175449534484 39.0 35.0 39.0 30.0 39.0 14-15 37.028398749333796 39.0 36.0 41.0 31.0 41.0 16-17 36.90370635202245 39.0 36.0 41.0 31.0 41.0 18-19 36.85051083953806 39.0 36.0 41.0 30.5 41.0 20-21 36.75625943638137 39.0 36.0 41.0 30.0 41.0 22-23 36.61480655328404 39.0 36.0 40.5 30.0 41.0 24-25 36.75139435778017 39.0 36.0 41.0 30.0 41.0 26-27 36.730357422472636 39.0 36.0 41.0 30.0 41.0 28-29 36.63115887741719 39.0 36.0 41.0 30.0 41.0 30-31 36.451924914058 39.0 35.5 41.0 30.0 41.0 32-33 36.27009922296652 39.0 35.0 40.0 30.0 41.0 34-35 36.038564003625865 38.5 35.0 40.0 29.0 41.0 36-37 35.84789235593851 38.0 35.0 40.0 28.0 41.0 38-39 35.64334752172432 38.0 35.0 40.0 27.0 41.0 40-41 35.46556106874067 38.0 34.5 40.0 27.0 41.0 42-43 35.23599949864173 38.0 34.0 40.0 26.0 41.0 44-45 34.96701360706441 38.0 34.0 40.0 25.5 41.0 46-47 34.688424513976976 38.0 33.0 40.0 24.0 41.0 48-49 34.5878899712545 38.0 33.5 40.0 24.0 41.0 50-51 33.66875787412603 37.5 32.0 39.5 23.0 40.5 52-53 33.8215559632184 37.5 33.0 39.5 23.0 40.5 54-55 34.242472443252424 38.0 33.0 40.0 23.0 41.0 56-57 34.14911206434624 38.0 33.0 40.0 23.0 41.0 58-59 33.87492027110697 38.0 33.0 40.0 22.0 41.0 60-61 33.536174650808995 37.0 33.0 40.0 21.5 41.0 62-63 33.20969542844583 37.0 32.0 40.0 20.0 41.0 64-65 32.86935092061732 36.0 32.0 39.0 19.5 41.0 66-67 32.47043602366871 36.0 31.0 39.0 18.0 41.0 68-69 32.053219036099236 35.0 31.0 39.0 16.5 40.0 70-71 31.599124741958516 35.0 31.0 38.0 13.0 40.0 72-73 31.19278284722651 35.0 30.5 37.0 10.0 39.5 74-75 30.75938376748469 35.0 30.0 37.0 8.0 39.0 76-77 30.31634431532993 34.0 29.5 36.0 7.0 39.0 78-79 29.86023812362719 34.0 29.0 36.0 3.5 38.5 80-81 29.364544561268424 34.0 29.0 35.0 2.0 37.0 82-83 28.872043207313798 34.0 28.5 35.0 2.0 37.0 84-85 28.545559136572003 34.0 28.0 35.0 2.0 36.0 86-87 28.217999623622163 34.0 27.0 35.0 2.0 36.0 88-89 27.80419571503888 33.5 26.5 35.0 2.0 35.5 90-91 27.370884444821144 33.0 26.0 35.0 2.0 35.0 92-93 26.975056887642594 33.0 25.5 35.0 2.0 35.0 94-95 26.485425953406722 33.0 25.0 35.0 2.0 35.0 96-97 25.87508332028461 32.5 23.5 35.0 2.0 35.0 98-99 25.1374834975557 32.0 19.0 35.0 2.0 35.0 100-101 22.61923635522598 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4428077409507267 1101 2 0.44923943633260066 1101 3 0.5636600439123463 1101 4 0.5539166480269344 1101 5 0.5736014759467594 1101 6 0.39639667817233715 1101 7 0.48692997328314647 1101 8 0.6360547642162828 1101 9 0.6877513957476395 1101 10-11 0.5919985047757379 1101 12-13 0.7225224625322753 1101 14-15 0.8995907305936299 1101 16-17 0.8436632874284342 1101 18-19 0.9149966311344073 1101 20-21 0.912690382363472 1101 22-23 0.8328337555501193 1101 24-25 0.8309114692599877 1101 26-27 0.8622979260995081 1101 28-29 0.8103416060963795 1101 30-31 0.8251800657820709 1101 32-33 0.7469267600959313 1101 34-35 0.625726114653034 1101 36-37 0.697631727155887 1101 38-39 0.7305325127335962 1101 40-41 0.740639242215174 1101 42-43 0.6662882622790818 1101 44-45 0.6442327768325811 1101 46-47 0.8173859881684606 1101 48-49 0.8518199020020134 1101 50-51 0.6621105341370708 1101 52-53 0.7635604049805877 1101 54-55 0.7678470120558742 1101 56-57 0.6526509552357282 1101 58-59 0.6394734634101553 1101 60-61 0.6834013328468203 1101 62-63 0.7143117224555837 1101 64-65 0.7040225500235522 1101 66-67 0.7725947850556025 1101 68-69 0.7322525572809653 1101 70-71 0.7549611822445819 1101 72-73 0.5626667920484216 1101 74-75 0.4855239643844058 1101 76-77 0.6122264599129714 1101 78-79 0.45056185738518195 1101 80-81 0.6100201534730907 1101 82-83 0.5861855416736574 1101 84-85 0.6359789846854724 1101 86-87 0.47075191208188016 1101 88-89 0.43442017601499217 1101 90-91 0.2284153325326237 1101 92-93 0.3438974007679647 1101 94-95 0.368432402962096 1101 96-97 0.44433916694078945 1101 98-99 0.47823004481073994 1101 100-101 0.9311488701970809 1102 1 0.24228580432562907 1102 2 0.33684239920144776 1102 3 0.3122404517336541 1102 4 0.38002419992776737 1102 5 0.349305514652265 1102 6 0.3544843134956679 1102 7 0.36687740241272593 1102 8 0.28570286894508 1102 9 0.5438628586988798 1102 10-11 0.4810281076552272 1102 12-13 0.3865907292222417 1102 14-15 0.4225575303418907 1102 16-17 0.4466279430475453 1102 18-19 0.47031824058128535 1102 20-21 0.5509573304140005 1102 22-23 0.368594554119106 1102 24-25 0.2076801276505762 1102 26-27 0.18352937094476118 1102 28-29 0.37804556141908563 1102 30-31 0.2565777600491046 1102 32-33 0.2592927272073666 1102 34-35 0.00700072002408092 1102 36-37 0.10868133793689339 1102 38-39 0.11144271541335371 1102 40-41 0.22296715103410492 1102 42-43 0.2959086124471355 1102 44-45 -0.035339528959944744 1102 46-47 0.006887497992742908 1102 48-49 0.067747307347652 1102 50-51 0.19318482225641276 1102 52-53 0.1587380996843919 1102 54-55 0.19922137899181536 1102 56-57 0.2658610739371028 1102 58-59 0.3590891679650383 1102 60-61 0.23914442249722612 1102 62-63 0.32622583420045004 1102 64-65 0.24561111149157 1102 66-67 0.3231946448930785 1102 68-69 0.1435981770037813 1102 70-71 0.17877570713136848 1102 72-73 0.2845573580786649 1102 74-75 -0.07845029812450477 1102 76-77 0.17288933332398315 1102 78-79 0.1111604069215133 1102 80-81 0.24459334272623678 1102 82-83 0.21782147251358097 1102 84-85 0.3266961958881467 1102 86-87 0.22046156125867356 1102 88-89 0.26031500250928374 1102 90-91 0.22373765668921664 1102 92-93 0.15538556537354964 1102 94-95 0.43377662503035097 1102 96-97 0.2879007242932694 1102 98-99 0.571378658770211 1102 100-101 0.7987344020900089 1103 1 0.4190694044834835 1103 2 0.47125854461410555 1103 3 0.36456924754720177 1103 4 0.48871242393430947 1103 5 0.474527388994737 1103 6 0.4090539116929435 1103 7 0.36350960539005683 1103 8 0.4021418839228659 1103 9 0.6523998212723399 1103 10-11 0.6159056004388006 1103 12-13 0.6405536333253963 1103 14-15 0.4753215421441652 1103 16-17 0.6571520542169438 1103 18-19 0.4978549966247101 1103 20-21 0.5891940875240138 1103 22-23 0.5697293542358537 1103 24-25 0.6789087001595888 1103 26-27 0.527939582255037 1103 28-29 0.26043315526662525 1103 30-31 0.4180639811991398 1103 32-33 0.6243534693386223 1103 34-35 0.45568232149480536 1103 36-37 0.4837690162387318 1103 38-39 0.6654639424344637 1103 40-41 0.572398175335799 1103 42-43 0.5088558154196221 1103 44-45 0.39292619083600755 1103 46-47 0.5547935452503125 1103 48-49 0.5781589420667999 1103 50-51 0.6058259632330376 1103 52-53 0.6564642815840145 1103 54-55 0.7327530328707539 1103 56-57 0.7970700406372799 1103 58-59 0.613187480139544 1103 60-61 0.589584001796517 1103 62-63 0.6876235771083685 1103 64-65 0.6704243375585648 1103 66-67 0.5457352164091986 1103 68-69 0.5394598678157152 1103 70-71 0.6988177500527648 1103 72-73 0.679956441007743 1103 74-75 0.5533900880963571 1103 76-77 0.6086896178118302 1103 78-79 0.6938218227479211 1103 80-81 0.7192659732304314 1103 82-83 0.5745322441699017 1103 84-85 0.6700088406652185 1103 86-87 0.7840158461750981 1103 88-89 0.8599811607694647 1103 90-91 0.8649213452768301 1103 92-93 0.7967521736364986 1103 94-95 0.694736388519523 1103 96-97 0.8994321784295387 1103 98-99 0.8132063235166989 1103 100-101 1.2514090629880563 1104 1 0.1602049408756514 1104 2 0.2373623543137917 1104 3 0.2728934473665703 1104 4 0.3311897530947192 1104 5 0.4564153020539763 1104 6 0.3811698700620383 1104 7 0.41309720126812977 1104 8 0.23957848532946002 1104 9 0.3814475783538356 1104 10-11 0.4793251194585153 1104 12-13 0.2500683249899751 1104 14-15 0.45549838641360907 1104 16-17 0.3090845571199381 1104 18-19 0.613220235223352 1104 20-21 0.6292482387323162 1104 22-23 0.31568545162112827 1104 24-25 0.36613268174657776 1104 26-27 0.04236657979790692 1104 28-29 0.32714935349884655 1104 30-31 0.34930553496185013 1104 32-33 0.15546201779312696 1104 34-35 0.07188491798559937 1104 36-37 0.2266467334948672 1104 38-39 0.16673391538554228 1104 40-41 0.37005901427740895 1104 42-43 0.3219704932862584 1104 44-45 0.3193951143048821 1104 46-47 0.37427200016276885 1104 48-49 0.29716745696028823 1104 50-51 0.19868607095990853 1104 52-53 0.3501889714590547 1104 54-55 0.5012054944790236 1104 56-57 0.5571521155277139 1104 58-59 0.5304019194199299 1104 60-61 0.7163283981450945 1104 62-63 0.8542197770710587 1104 64-65 0.4176194864000635 1104 66-67 0.6346980723089359 1104 68-69 0.5671353203796627 1104 70-71 0.5893459387354447 1104 72-73 0.5302530491341635 1104 74-75 0.43393301618372604 1104 76-77 0.6683848262093655 1104 78-79 0.5304219238693086 1104 80-81 0.5269697230119839 1104 82-83 0.5826122832920255 1104 84-85 0.5195100183501324 1104 86-87 0.6195744313065958 1104 88-89 0.6066436404084463 1104 90-91 0.6772262039632189 1104 92-93 0.5323693428513039 1104 94-95 0.3905695527137105 1104 96-97 0.4885917121886685 1104 98-99 0.790929125512978 1104 100-101 1.1808851852096254 1105 1 0.29840557052948213 1105 2 0.23412498320588782 1105 3 0.2736429520645167 1105 4 0.32254977508485183 1105 5 0.3019404620007222 1105 6 0.24222206517319478 1105 7 0.2536332339831091 1105 8 0.3513001098503139 1105 9 0.5143102437020275 1105 10-11 0.4880294488818535 1105 12-13 0.34191089522619933 1105 14-15 0.17735760066246797 1105 16-17 0.2763125078797515 1105 18-19 0.2514767719875195 1105 20-21 0.2513910068564158 1105 22-23 0.01077593378500552 1105 24-25 0.3243569670964632 1105 26-27 0.3151536773686203 1105 28-29 0.16564652378374944 1105 30-31 0.14774759015381989 1105 32-33 0.19414595276850122 1105 34-35 0.2289501208177569 1105 36-37 0.15986365191823637 1105 38-39 0.17454693405502297 1105 40-41 -0.00965596428570592 1105 42-43 0.16857070037551125 1105 44-45 0.07029836355624752 1105 46-47 0.24957089937505827 1105 48-49 0.38699766240727485 1105 50-51 0.4796321124453087 1105 52-53 0.3570728256020601 1105 54-55 0.4379926060265191 1105 56-57 0.7288387748099865 1105 58-59 0.6188487150689639 1105 60-61 0.35941274683307967 1105 62-63 0.4648640249864755 1105 64-65 0.5831447162306986 1105 66-67 0.7214627045391495 1105 68-69 0.7359060744384678 1105 70-71 0.7686612979328054 1105 72-73 0.6281726057907768 1105 74-75 0.7082590152030193 1105 76-77 0.5433881748123319 1105 78-79 0.6184587340425409 1105 80-81 0.41748525645316903 1105 82-83 0.5897171452305834 1105 84-85 0.7029115467107623 1105 86-87 0.7411636608017886 1105 88-89 0.5683370784278416 1105 90-91 0.5897225496182443 1105 92-93 0.5601304261447453 1105 94-95 0.4311211345937487 1105 96-97 0.7415920604433737 1105 98-99 0.7455022689019053 1105 100-101 1.1686406544974055 1106 1 0.24439030762952996 1106 2 0.26514087622556204 1106 3 0.2411007690497442 1106 4 0.27277731831806307 1106 5 0.27044847852386056 1106 6 0.19952795329193407 1106 7 0.4292335980522779 1106 8 0.3617390932571638 1106 9 0.6232509424347867 1106 10-11 0.5449047551900534 1106 12-13 0.6278312200879199 1106 14-15 0.7496008268754437 1106 16-17 0.7814024749734969 1106 18-19 0.7547638965411849 1106 20-21 0.5224650109171805 1106 22-23 0.5372059554891351 1106 24-25 0.3756802185142334 1106 26-27 0.12030315576951978 1106 28-29 0.5572799341317207 1106 30-31 0.46194628790639314 1106 32-33 0.37572895353865476 1106 34-35 0.4222428464356227 1106 36-37 0.23679076807030697 1106 38-39 0.3478333584756683 1106 40-41 0.20571895694932607 1106 42-43 0.07742038181498145 1106 44-45 0.26448025811621534 1106 46-47 0.4843745120614713 1106 48-49 0.4006187504139973 1106 50-51 0.35551603038972246 1106 52-53 0.5401436090509222 1106 54-55 0.6637259953704913 1106 56-57 0.49157069387460695 1106 58-59 0.47271695003360037 1106 60-61 0.5774131669128977 1106 62-63 0.6551146726095212 1106 64-65 0.5778412085642444 1106 66-67 0.7047715332166007 1106 68-69 0.7216023567320988 1106 70-71 0.7234362051651821 1106 72-73 0.8732541712882202 1106 74-75 0.6111995739091753 1106 76-77 0.7346120073405089 1106 78-79 0.8802629768486732 1106 80-81 0.8391788782413094 1106 82-83 0.9801148207889376 1106 84-85 0.9032791165490295 1106 86-87 1.0957075241358218 1106 88-89 1.1775508290404595 1106 90-91 1.3226218004759112 1106 92-93 1.327308189919865 1106 94-95 1.213407769054296 1106 96-97 1.4613988237283984 1106 98-99 1.6667653088795014 1106 100-101 2.0565948657975497 1107 1 0.15357067559941484 1107 2 0.11259826249548155 1107 3 0.08911478424335684 1107 4 0.07268089281159007 1107 5 0.15479866957323907 1107 6 0.10613010621974439 1107 7 0.18147658220855334 1107 8 0.2914445945353066 1107 9 0.13506991826395875 1107 10-11 0.18691130907946985 1107 12-13 0.18158295891893061 1107 14-15 0.11357356789227424 1107 16-17 0.020121477345057315 1107 18-19 0.262042398271511 1107 20-21 0.33929841758465074 1107 22-23 0.28199123872382614 1107 24-25 0.4040961555189426 1107 26-27 0.11975266762807735 1107 28-29 0.18731267312868027 1107 30-31 0.20811695191780188 1107 32-33 -0.024127727708574298 1107 34-35 0.24493496537598247 1107 36-37 0.3167985633112522 1107 38-39 0.2906102506612953 1107 40-41 0.1225224209595197 1107 42-43 0.26732960481317036 1107 44-45 0.29673991881007566 1107 46-47 0.240867780292362 1107 48-49 0.22529951854972552 1107 50-51 0.10699121376311638 1107 52-53 0.40785654321310716 1107 54-55 0.43015657179709876 1107 56-57 0.4366350020748797 1107 58-59 0.5036409652119929 1107 60-61 0.382058034310063 1107 62-63 0.4234650301197789 1107 64-65 0.550028032475538 1107 66-67 0.5960781140544071 1107 68-69 0.5840774066322965 1107 70-71 0.6677559054544098 1107 72-73 0.43065906655743547 1107 74-75 0.5163094152486174 1107 76-77 0.4572983407504765 1107 78-79 0.4217852716945245 1107 80-81 0.4046430173417015 1107 82-83 0.5841890014385704 1107 84-85 0.4823715799821926 1107 86-87 0.49327206959359415 1107 88-89 0.45010493263088946 1107 90-91 0.5402227034246501 1107 92-93 0.5836124641580653 1107 94-95 0.4937989825591451 1107 96-97 0.6040502105048091 1107 98-99 0.924462525684131 1107 100-101 1.2580572347286427 1108 1 0.06331555724798932 1108 2 0.1300492486595637 1108 3 0.14239469353680434 1108 4 0.21660467164191033 1108 5 0.27690400683779615 1108 6 0.12503852335706966 1108 7 -0.05784367033870552 1108 8 0.12033550809132976 1108 9 0.1923279142181471 1108 10-11 -0.09775110317875857 1108 12-13 0.2203561293438625 1108 14-15 0.2499198378467753 1108 16-17 0.3043518066071229 1108 18-19 0.21041537626616247 1108 20-21 0.21993574720880815 1108 22-23 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88-89 -3.9201689218145326 2316 90-91 -3.6355249573212767 2316 92-93 -4.115118995046103 2316 94-95 -4.4374675267631005 2316 96-97 -5.358675416599283 2316 98-99 -6.428878110971915 2316 100-101 -6.9140331976642475 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6920.0 3 2912.0 4 3034.0 5 3297.0 6 3665.0 7 4288.0 8 5402.0 9 6848.0 10 8386.0 11 10543.0 12 12658.0 13 15024.0 14 17202.0 15 20022.0 16 23316.0 17 28114.0 18 24591.0 19 19967.0 20 20445.0 21 22240.0 22 24863.0 23 27844.0 24 31404.0 25 36694.0 26 42787.0 27 49587.0 28 58991.0 29 70489.0 30 83929.0 31 102148.0 32 125721.0 33 158878.0 34 204176.0 35 271572.0 36 368402.0 37 466548.0 38 352231.0 39 49277.0 40 21.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.82067605289724 18.28852779404421 14.215109699623858 22.675686453434693 2 15.434464307011003 19.95868231723876 39.960758618806835 24.646094756943395 3 20.02714205250816 24.247091820313578 29.349266058209572 26.37650006896869 4 12.804468125548176 16.22088533935829 37.32781285268789 33.64683368240564 5 13.736965036472744 37.49991016565453 33.83014121959108 14.93298357828165 6 31.984292409255392 35.60951351957323 17.465622128889486 14.940571942281897 7 28.927720224661368 32.65606264819713 20.28136209667654 18.134855030464962 8 26.653365583609272 33.73610248804268 19.64202049990802 19.96851142844003 9 26.41743923720583 17.122915335035664 20.498763851208 35.9608815765505 10-11 25.218785337582496 26.342796693758743 26.929542048287104 21.508875920371658 12-13 26.554879127784847 23.77065834904236 27.39595349397889 22.278509029193906 14-15 23.07856471514758 25.217329133097838 26.311411917210137 25.392694234544443 16-17 22.147694775341517 28.598957058896023 26.786673522353382 22.466674643409075 18-19 22.06809148861782 27.293836020310525 28.921024178662453 21.717048312409204 20-21 23.08470904696149 25.963304844778413 28.885271921757134 22.066714186502967 22-23 22.466837160340987 26.136306693202805 28.91408796331303 22.482768183143182 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26.82348301889435 27.77007714578724 22.627557392454076 50-51 22.32717514544306 27.045226246155845 28.077522703985707 22.550075904415383 52-53 22.807441280809673 27.230781524835844 27.36900136747317 22.592775826881308 54-55 22.34405632682835 26.83612699360205 27.706756270673203 23.113060408896395 56-57 22.72313053185952 27.08277732618723 27.409485029922916 22.784607112030336 58-59 23.233853182670412 26.73272536582212 27.34702637080032 22.686395080707147 60-61 22.692628465071106 26.8661404482961 28.135885310447527 22.305345776185266 62-63 22.488327323401382 26.987605413248666 27.970803942132257 22.553263321217692 64-65 22.29233619968435 27.36661609386035 27.643386834921056 22.69766087153424 66-67 22.45584621473821 27.149379041938104 27.29834217235115 23.096432570972535 68-69 22.323640378187427 27.018170317247336 27.689641045940437 22.9685482586248 70-71 22.702759974355892 26.9139072609827 27.4091726182673 22.974160146394112 72-73 22.553799442530785 26.811478650997767 27.77850205917391 22.856219847297538 74-75 22.22412572299359 27.600006967531126 27.375645282272103 22.800222027203183 76-77 22.44471178150098 27.057173914487937 27.354560949167467 23.14355335484361 78-79 22.53527829356856 27.147836611847186 27.374357556145373 22.942527538438885 80-81 22.676133358595404 27.285192325478448 27.23553619195822 22.80313812396793 82-83 22.63919420069882 27.17902542734325 27.534343425817898 22.647436946140033 84-85 22.430637023712112 27.314080786557938 27.299138139741046 22.9561440499889 86-87 22.475416309681858 27.338532576498604 27.27334599832526 22.912705115494276 88-89 22.367995965896164 27.3190574951383 26.91916689578188 23.39377964318366 90-91 22.454408290962323 26.94113814690987 27.32875342137596 23.275700140751844 92-93 22.785284813366182 27.018790717321707 27.420266158425648 22.77565831088646 94-95 22.368106299404914 26.867419551343758 27.494472252792253 23.270001896459082 96-97 22.011451854012208 26.884344319939313 27.780138338856208 23.324065487192268 98-99 22.1431106474816 27.157745151287866 27.129654375695083 23.56948982553545 100-101 22.00194472043252 26.807628060810508 27.384703101403357 23.80572411735362 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 187.0 1 158.5 2 189.5 3 251.0 4 233.0 5 255.5 6 428.5 7 875.5 8 1478.0 9 1848.5 10 1802.0 11 1739.0 12 1935.5 13 2156.0 14 2375.0 15 2542.0 16 2702.0 17 2994.5 18 3256.5 19 3588.0 20 4008.5 21 4578.0 22 5479.0 23 6581.0 24 8008.0 25 9954.5 26 12533.5 27 15960.0 28 19542.5 29 23925.0 30 29722.5 31 36009.0 32 42825.0 33 48326.0 34 53465.0 35 58401.5 36 64009.0 37 70193.5 38 73952.0 39 76565.5 40 79496.5 41 84651.0 42 89440.0 43 93450.0 44 98269.0 45 103619.5 46 106217.0 47 112758.5 48 131037.5 49 130490.5 50 111833.5 51 100945.5 52 94816.0 53 92100.0 54 89411.0 55 83659.5 56 76911.5 57 69855.0 58 63943.5 59 57139.5 60 47888.5 61 39072.5 62 31838.0 63 24878.0 64 18550.0 65 14454.0 66 10979.0 67 8423.5 68 6223.5 69 4239.0 70 3133.0 71 2369.5 72 1833.5 73 1548.0 74 1234.0 75 977.5 76 705.0 77 384.0 78 214.0 79 152.0 80 101.5 81 70.0 82 44.0 83 33.5 84 27.0 85 17.0 86 11.5 87 9.5 88 7.5 89 7.0 90 4.5 91 5.5 92 5.0 93 1.5 94 1.0 95 2.0 96 2.0 97 2.5 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03325628601267905 2 0.022984906099475802 3 0.02025544850016305 4 0.04830421672467961 5 0.05516377463874192 6 0.04751411057751013 7 0.05038722383994461 8 0.04324035459963885 9 0.04291712935761497 10-11 0.052326575292087875 12-13 0.04559271608325707 14-15 0.03950530735847403 16-17 0.04830421672467961 18-19 0.051895608302722704 20-21 0.01204911874433458 22-23 0.02009383587915111 24-25 0.006069451766892829 26-27 0.011241055639274885 28-29 0.006069451766892829 30-31 0.009068263734558811 32-33 0.0038966598621767564 34-35 0.008565468913632778 36-37 0.009858369881728293 38-39 0.011384711302396608 40-41 0.00210096407315521 42-43 0.002136877988935641 44-45 0.002262576694167149 46-47 6.284935261575414E-4 48-49 0.0011672022628640055 50-51 4.668809051456022E-4 52-53 0.0037170902832746023 54-55 0.006662031377269939 56-57 0.01612534818541349 58-59 0.010630519071007557 60-61 0.010558691239446695 62-63 0.005602570861747226 64-65 5.3870873670646405E-5 66-67 0.006823643998281879 68-69 0.01696932520625362 70-71 0.006141279598453691 72-73 0.002406232357288873 74-75 0.0033220372096898617 76-77 0.006662031377269939 78-79 0.005243431703942917 80-81 0.010361164702654326 82-83 0.006087408724783044 84-85 0.016448573427437368 86-87 0.004327626851541928 88-89 0.006266978303685199 90-91 0.0036991333253843864 92-93 0.016340831680096077 94-95 0.010774174734129281 96-97 0.009265790271351182 98-99 0.02169200513138029 100-101 0.036003700569882016 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2784436.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.158404554280615 #Duplication Level Percentage of deduplicated Percentage of total 1 77.64387673540288 43.60356240869452 2 12.900108546975073 14.488990291503184 3 4.068902900121703 6.855092854713607 4 1.7946020077203424 4.0312794225393285 5 0.9692959878865676 2.7217058110287478 6 0.5670090984520083 1.9105395802095484 7 0.38693849459189766 1.521089396184128 8 0.2814280973664523 1.2643642355877363 9 0.19154273571982533 0.9681060997788846 >10 1.0607664136778379 11.091401351714095 >50 0.08642424092239634 3.3238286198635283 >100 0.045121480295073804 4.551284895779963 >500 0.0024413534352094347 0.9843238903963081 >1k 0.0012849228606140461 1.5355481553672237 >5k 1.9273842909210695E-4 0.7839016628680374 >10k+ 6.424614303070231E-5 0.36498132377102527 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10116 0.36330517203483936 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8502 0.30534011196522387 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6916 0.24838064153746034 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6309 0.22658089465873876 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4701 0.16883131808380583 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4024 0.14451759710045411 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3959 0.14218319257472609 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3028 0.10874733698314487 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.591391578043094E-5 0.0 5 0.0 0.0 0.0 7.182783156086188E-5 0.0 6 0.0 0.0 0.0 1.0774174734129281E-4 0.0 7 0.0 0.0 0.0 1.0774174734129281E-4 0.0 8 3.591391578043094E-5 0.0 0.0 1.0774174734129281E-4 0.0 9 3.591391578043094E-5 0.0 0.0 1.0774174734129281E-4 0.0 10-11 3.591391578043094E-5 0.0 0.0 1.0774174734129281E-4 0.0 12-13 3.591391578043094E-5 0.0 0.0 1.0774174734129281E-4 0.0 14-15 3.591391578043094E-5 0.0 0.0 1.0774174734129281E-4 0.0 16-17 3.591391578043094E-5 0.0 0.0 3.591391578043094E-4 0.0 18-19 3.591391578043094E-5 0.0 0.0 4.130100314749558E-4 0.0 20-21 3.591391578043094E-5 3.591391578043094E-5 0.0 4.489239472553867E-4 0.0 22-23 3.591391578043094E-5 3.591391578043094E-5 0.0 4.668809051456022E-4 0.0 24-25 3.591391578043094E-5 3.591391578043094E-5 0.0 5.027948209260331E-4 0.0 26-27 3.591391578043094E-5 3.591391578043094E-5 0.0 5.207517788162485E-4 0.0 28-29 3.591391578043094E-5 3.591391578043094E-5 0.0 0.001185159220754221 0.0 30-31 3.591391578043094E-5 3.591391578043094E-5 0.0 0.0025319310625203815 0.0 32-33 3.591391578043094E-5 3.591391578043094E-5 0.0 0.004291712935761497 0.0 34-35 3.591391578043094E-5 8.978478945107735E-5 0.0 0.005656441735417872 0.0 36-37 3.591391578043094E-5 1.0774174734129281E-4 0.0 0.007631707103341575 0.0 38-39 3.591391578043094E-5 1.0774174734129281E-4 0.0 0.011187184765604237 0.0 40-41 3.591391578043094E-5 1.0774174734129281E-4 0.0 0.015586639448707026 0.0 42-43 3.591391578043094E-5 1.0774174734129281E-4 0.0 0.02029136241594348 0.0 44-45 3.591391578043094E-5 1.0774174734129281E-4 0.0 0.023038776973146446 0.0 46-47 3.591391578043094E-5 1.0774174734129281E-4 0.0 0.02639672809861674 0.0 48-49 3.591391578043094E-5 1.0774174734129281E-4 0.0 0.031550375013108575 0.0 50-51 3.591391578043094E-5 1.0774174734129281E-4 0.0 0.0372606876221971 0.0 52-53 3.591391578043094E-5 1.2569870523150827E-4 0.0 0.04887883937716651 0.0 54-55 3.591391578043094E-5 1.4365566312172376E-4 0.0 0.058665381427333935 0.0 56-57 3.591391578043094E-5 1.795695789021547E-4 0.0 0.07951340953787409 0.0 58-59 3.591391578043094E-5 1.795695789021547E-4 0.0 0.11257216901376077 0.0 60-61 3.591391578043094E-5 1.795695789021547E-4 0.0 0.14020792720680236 0.0 62-63 3.591391578043094E-5 1.795695789021547E-4 0.0 0.15523790096091272 0.0 64-65 3.591391578043094E-5 1.9752653679237016E-4 0.0 0.18217333779623593 0.0 66-67 3.591391578043094E-5 2.1548349468258562E-4 0.0 0.2421675341074458 0.0 68-69 3.591391578043094E-5 2.1548349468258562E-4 0.0 0.33008479993794076 0.0 70-71 3.591391578043094E-5 2.5139741046301654E-4 0.0 0.4025411250249602 0.0 72-73 3.591391578043094E-5 2.873113262434475E-4 0.0 0.48762119150880107 0.0 74-75 3.591391578043094E-5 2.873113262434475E-4 0.0 0.5867076851470101 0.0 76-77 3.591391578043094E-5 2.873113262434475E-4 0.0 0.6474201597738285 0.0 78-79 3.591391578043094E-5 2.873113262434475E-4 0.0 0.7338469980994355 0.0 80-81 3.591391578043094E-5 2.873113262434475E-4 0.0 0.8512675457435546 0.0 82-83 3.591391578043094E-5 2.873113262434475E-4 0.0 0.9579498325693245 0.0 84-85 3.591391578043094E-5 2.873113262434475E-4 0.0 1.1095065571627432 0.0 86-87 3.591391578043094E-5 3.2322524202387843E-4 0.0 1.3265702641396677 0.0 88-89 3.591391578043094E-5 3.591391578043094E-4 0.0 1.6222854466757362 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12720 0.0 25.553173 1 TACCTGG 2455 0.0 25.164663 2 GTACCTG 2595 0.0 24.904474 1 TACATGG 13085 0.0 24.36958 2 ACATGGG 12910 0.0 23.669222 3 TAGCGCG 190 3.2725984E-7 22.51268 4 GAGTACT 9275 0.0 22.467913 12-13 CATGGGG 5775 0.0 22.13801 4 GTACTTT 9740 0.0 21.053345 14-15 AGTACTT 9795 0.0 19.868109 12-13 GTGTAGC 1585 0.0 18.58824 1 GTATCAA 24990 0.0 18.521175 1 TAGCCCT 1860 0.0 18.397459 4 GTAGCGC 310 5.5588316E-9 18.395805 3 AGAGTAC 17665 0.0 18.319813 10-11 ACTTTTT 11825 0.0 17.962172 16-17 CGCGCGT 270 4.8572474E-7 17.600294 7 CATGGGA 7545 0.0 17.322546 4 TAGCGCC 220 2.9055102E-5 17.282461 4 TACTTTT 11355 0.0 17.09638 14-15 >>END_MODULE