FastQCFastQC Report
Thu 26 May 2016
SRR936577_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936577_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2784436
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT119280.42838118742898024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97590.35048390410122554No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87040.3125947229528709No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54900.19716739763456587No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49750.1786717310076439No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44540.1599605808860394No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38550.13844814533356126No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT34800.12498042691589967No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31250.11223098681384669No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28390.10195960690064343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG130000.023.0251221
TACATGG133800.022.1154732
ACATGGG131000.021.8626373
GAGTACT111000.021.6972112-13
GTACTTT117450.020.34388414-15
GTACCTG24200.019.633121
AGTACTT115400.019.61444512-13
GTATCAA279650.018.2301241
AGAGTAC198900.018.16314910-11
GTGTAGC14550.017.959921
CATGGGA75150.017.8850674
TACCTGG24700.017.8834082
ATAACGC2152.3981705E-517.6729093
ACTTTTT137850.017.55487816-17
CGTAACG1102.9189878E-517.27373114-15
TACTTTT130350.017.25551214-15
CATGGGG58750.016.895524
GGACCGT3401.7558705E-816.7620686
GTACCGT2606.639577E-616.4397246
TAACGCA2901.03204E-616.377034
Produced by FastQC (version 0.11.5)