##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936577_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2784436 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.866326968908606 31.0 31.0 34.0 30.0 34.0 2 32.013391940055364 31.0 31.0 34.0 30.0 34.0 3 32.12152981788772 33.0 31.0 34.0 30.0 34.0 4 35.866942174285924 37.0 35.0 37.0 35.0 37.0 5 35.6470552743895 37.0 35.0 37.0 33.0 37.0 6 35.74520764707826 37.0 35.0 37.0 33.0 37.0 7 35.69416068460543 37.0 35.0 37.0 33.0 37.0 8 35.66431227006115 37.0 35.0 37.0 33.0 37.0 9 37.296463628540934 39.0 37.0 39.0 34.0 39.0 10-11 37.1649689560112 39.0 37.0 39.0 33.0 39.0 12-13 37.07014239149329 39.0 37.0 39.0 33.0 39.0 14-15 38.16923391307971 40.0 37.0 41.0 33.0 41.0 16-17 38.09999547484661 40.0 37.0 41.0 33.0 41.0 18-19 38.24948876540887 40.0 37.5 41.0 33.5 41.0 20-21 38.282680585942714 40.0 38.0 41.0 34.0 41.0 22-23 38.19684435196212 40.0 38.0 41.0 33.0 41.0 24-25 38.07708688581817 40.0 37.0 41.0 33.0 41.0 26-27 37.865194064435315 40.0 37.0 41.0 33.0 41.0 28-29 37.79235597442354 40.0 37.0 41.0 32.5 41.0 30-31 37.7757610517893 40.0 37.0 41.0 33.0 41.0 32-33 37.640721855341624 40.0 37.0 41.0 33.0 41.0 34-35 37.476478360429184 40.0 36.0 41.0 32.0 41.0 36-37 37.222179464710266 39.0 36.0 41.0 31.5 41.0 38-39 37.07449910861661 39.0 36.0 41.0 31.0 41.0 40-41 36.8806370482209 39.0 35.5 41.0 31.0 41.0 42-43 36.63791877421496 39.0 35.0 40.5 30.0 41.0 44-45 36.39985009531553 39.0 35.0 40.0 30.0 41.0 46-47 36.06507439926793 39.0 35.0 40.0 29.5 41.0 48-49 35.71890249946488 39.0 35.0 40.0 28.5 41.0 50-51 35.40832811384424 38.0 34.5 40.0 27.5 41.0 52-53 35.14225843222829 38.0 34.0 40.0 27.0 41.0 54-55 34.87736421307582 38.0 34.0 40.0 26.0 41.0 56-57 34.55696431880639 38.0 34.0 40.0 26.0 41.0 58-59 34.21747976969124 37.5 33.0 40.0 25.0 41.0 60-61 33.84358502044938 37.0 33.0 40.0 24.0 41.0 62-63 33.456560143598196 36.0 32.0 39.0 23.0 40.5 64-65 33.044932079602475 36.0 32.0 39.0 22.5 40.0 66-67 32.57665035217186 35.5 31.0 38.5 22.0 40.0 68-69 32.29186323549904 35.0 31.0 38.0 21.5 40.0 70-71 31.850728837006848 35.0 31.0 37.5 20.0 40.0 72-73 31.395282922645734 35.0 30.5 37.0 18.5 39.0 74-75 30.87085194272736 34.0 30.0 36.5 17.5 39.0 76-77 29.440825538816476 32.5 28.5 35.0 14.5 37.0 78-79 30.227831776345372 34.0 29.5 35.0 16.0 37.5 80-81 30.022894762170864 34.0 30.0 35.0 11.5 37.0 82-83 29.685612813510527 34.0 29.0 35.0 7.5 36.5 84-85 29.267394545969093 34.0 29.0 35.0 7.0 36.0 86-87 28.899533873287087 34.0 29.0 35.0 2.0 36.0 88-89 28.489377561560044 33.0 29.0 35.0 2.0 35.0 90-91 28.00217225319598 33.0 27.0 35.0 2.0 35.0 92-93 27.50685004072638 33.0 26.5 35.0 2.0 35.0 94-95 26.90786320820446 32.5 25.5 34.0 2.0 35.0 96-97 26.140804636917494 32.0 24.5 34.0 2.0 35.0 98-99 25.19503913898542 32.0 21.0 34.0 2.0 35.0 100-101 21.99937689356121 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17814225641902937 1101 2 0.2924236398731388 1101 3 0.2370298919014786 1101 4 0.12121966465659284 1101 5 0.13322135638172483 1101 6 0.13431502944585105 1101 7 0.11032126661029906 1101 8 0.14140209551131733 1101 9 0.20065309303352308 1101 10-11 0.1746375091801866 1101 12-13 0.2661478072441881 1101 14-15 0.3939237523191963 1101 16-17 0.33123714055040665 1101 18-19 0.2796309799393768 1101 20-21 0.1996327596046399 1101 22-23 0.1762959223938978 1101 24-25 0.0718119312889769 1101 26-27 0.12691375582251396 1101 28-29 0.082130149884172 1101 30-31 0.21313215578054212 1101 32-33 0.22568418499660226 1101 34-35 0.039893328988164 1101 36-37 0.08452558623715589 1101 38-39 0.0854881975558115 1101 40-41 0.033189894538530496 1101 42-43 0.055459333298081503 1101 44-45 0.1646465716717529 1101 46-47 0.18754683326013577 1101 48-49 0.06543558668429483 1101 50-51 0.06820212204117126 1101 52-53 0.23037444796850082 1101 54-55 0.1119164518153255 1101 56-57 0.060384969918871434 1101 58-59 -0.011587811720843888 1101 60-61 0.02112980545162202 1101 62-63 0.05590720076435929 1101 64-65 -0.07983567097607391 1101 66-67 -0.09622738340259929 1101 68-69 -0.18804606442812144 1101 70-71 -0.18620239168718555 1101 72-73 -0.16323276479828408 1101 74-75 -0.09236557828432979 1101 76-77 -0.07425775790372313 1101 78-79 -0.3215785359826562 1101 80-81 -0.18006006092856097 1101 82-83 0.07800687091912195 1101 84-85 0.05708253114761774 1101 86-87 -0.05783415203819686 1101 88-89 -0.21293262365747267 1101 90-91 -0.16474144729949458 1101 92-93 -0.17927685189146914 1101 94-95 -0.09192737107239779 1101 96-97 -0.023795562255205738 1101 98-99 0.37762953583354886 1101 100-101 0.7901893077740709 1102 1 0.2174056289356585 1102 2 0.2403167351816684 1102 3 0.22798524041181167 1102 4 0.10203487050291926 1102 5 0.04561918763479156 1102 6 0.12814152572043014 1102 7 0.16446171898184048 1102 8 0.10745795007583325 1102 9 0.17739941674540916 1102 10-11 0.19915314444794063 1102 12-13 0.20592596928872808 1102 14-15 0.3300078577706458 1102 16-17 0.31002515169193856 1102 18-19 0.30049484560571216 1102 20-21 0.005851429013674192 1102 22-23 0.10641240022498977 1102 24-25 0.13985050973134605 1102 26-27 0.03245146569404511 1102 28-29 -0.0012377608406453078 1102 30-31 0.14286020873259275 1102 32-33 0.23053110807200028 1102 34-35 0.11105211167869555 1102 36-37 0.290712093708855 1102 38-39 0.2815498492102648 1102 40-41 0.41950032473422283 1102 42-43 0.36894193507969675 1102 44-45 0.46507450411580464 1102 46-47 0.4351506696630807 1102 48-49 0.3963593887017609 1102 50-51 0.2880136769120156 1102 52-53 0.35176590837853894 1102 54-55 0.39917010030917055 1102 56-57 0.29101981082646944 1102 58-59 0.0647395134184734 1102 60-61 0.14929651546410838 1102 62-63 0.059446999176508086 1102 64-65 0.3231798084452535 1102 66-67 0.3542702332390206 1102 68-69 0.16856043426853518 1102 70-71 0.2104851272972894 1102 72-73 0.168891848811775 1102 74-75 -0.04901754046030149 1102 76-77 -0.268529756569599 1102 78-79 -0.1668083449367792 1102 80-81 0.29727788360599305 1102 82-83 0.04353463441373506 1102 84-85 0.04929695994565364 1102 86-87 -0.04511430435077557 1102 88-89 -0.07423754463241394 1102 90-91 -0.09245997475923673 1102 92-93 0.016338701780252762 1102 94-95 0.16739333704610715 1102 96-97 0.44418514455299984 1102 98-99 0.5878822452782373 1102 100-101 1.1058187188731132 1103 1 0.2097669265648321 1103 2 0.2164534018257065 1103 3 0.2047269527881923 1103 4 0.15706135579807778 1103 5 0.03198199981753902 1103 6 0.23950940419156552 1103 7 0.13126836711575152 1103 8 0.1162693164029065 1103 9 0.26450205247588343 1103 10-11 0.1275823914278007 1103 12-13 0.25619116160891764 1103 14-15 0.1849568700625781 1103 16-17 0.3450984116763962 1103 18-19 0.18517710448190172 1103 20-21 0.24266860727042427 1103 22-23 0.18987045959898552 1103 24-25 0.10977231920593056 1103 26-27 0.23323332246692274 1103 28-29 0.2894765324921167 1103 30-31 0.1650724653689437 1103 32-33 0.1564917393751699 1103 34-35 0.24872837730540454 1103 36-37 0.21117480825041213 1103 38-39 0.21090625120497464 1103 40-41 0.3216146395926529 1103 42-43 0.26882262277623425 1103 44-45 0.20202364827667907 1103 46-47 0.12282577174031672 1103 48-49 0.21114989646505933 1103 50-51 0.2603312832580116 1103 52-53 0.24809122517913096 1103 54-55 0.16444057223514363 1103 56-57 0.17782026305937393 1103 58-59 0.23160555105735625 1103 60-61 0.17028389386106824 1103 62-63 0.12535601736375668 1103 64-65 0.058005086945662754 1103 66-67 0.16340526024790591 1103 68-69 0.24390186332060892 1103 70-71 0.2875496276933802 1103 72-73 0.150270693736811 1103 74-75 0.07515025501096773 1103 76-77 -0.04079015506590267 1103 78-79 -0.0922079029818974 1103 80-81 0.14555762417324303 1103 82-83 0.2869992678423152 1103 84-85 0.29595994850311413 1103 86-87 0.11179567667366896 1103 88-89 0.04470634998103051 1103 90-91 -0.03140126108258556 1103 92-93 -0.02304114573658822 1103 94-95 0.23464121284107975 1103 96-97 0.15472365464727744 1103 98-99 0.3704570251492214 1103 100-101 0.7507313411548928 1104 1 0.15039670355462675 1104 2 0.16507988712946897 1104 3 0.1197026567834385 1104 4 0.04642034034571196 1104 5 -0.023352896647388377 1104 6 0.12564064206714676 1104 7 0.09844422361257443 1104 8 0.02417319352955616 1104 9 0.07072614440829739 1104 10-11 0.08335236142489322 1104 12-13 0.07443613951028993 1104 14-15 0.12791876620485 1104 16-17 0.21422168390810015 1104 18-19 0.04446562613714633 1104 20-21 0.02116026342187638 1104 22-23 0.1206726327368628 1104 24-25 0.06907577275098475 1104 26-27 0.14361263439553085 1104 28-29 0.08949459941175775 1104 30-31 -0.002621280904044454 1104 32-33 0.10138339407821206 1104 34-35 0.13689438294769474 1104 36-37 0.15550406337685274 1104 38-39 0.17261877030340855 1104 40-41 0.1702919428723888 1104 42-43 0.17630841651718043 1104 44-45 0.16507141374366086 1104 46-47 0.10955997400100159 1104 48-49 0.02366234367697473 1104 50-51 -0.024666989140442297 1104 52-53 -0.03213122472185148 1104 54-55 -0.004670845962778003 1104 56-57 0.06123036174325591 1104 58-59 0.0029551518603838645 1104 60-61 0.18797899324722778 1104 62-63 0.1788440096277597 1104 64-65 0.22614193014023698 1104 66-67 -0.002345577491141171 1104 68-69 0.354993217708774 1104 70-71 0.044139955907184 1104 72-73 0.04188527983949797 1104 74-75 0.13257147094896737 1104 76-77 0.06629951002715018 1104 78-79 0.15807996708264582 1104 80-81 0.25630688867999396 1104 82-83 0.24508706354280818 1104 84-85 0.18946117459517708 1104 86-87 0.21714578136841922 1104 88-89 0.28755338396646835 1104 90-91 0.31291234748158914 1104 92-93 0.37715027213353736 1104 94-95 0.36745060550480346 1104 96-97 0.5965019579157698 1104 98-99 0.6421680119899094 1104 100-101 1.277476433117993 1105 1 0.04065803435350546 1105 2 0.08177130433977098 1105 3 0.020764542460391056 1105 4 -0.010349253640548284 1105 5 -0.05084463578946696 1105 6 -0.023481674537151775 1105 7 0.0644340648247308 1105 8 0.028093679641877145 1105 9 -0.05378597869916035 1105 10-11 -0.09525775287020366 1105 12-13 -0.21591074081471362 1105 14-15 -0.0071303912866440555 1105 16-17 0.00961359857520705 1105 18-19 -0.018091809683241422 1105 20-21 0.04845720122637687 1105 22-23 0.10321330807146012 1105 24-25 0.042045558452045384 1105 26-27 0.05164048778379282 1105 28-29 -8.792444466720895E-4 1105 30-31 0.012806460902943684 1105 32-33 0.03861135648534031 1105 34-35 0.06942964228098703 1105 36-37 0.04139939827791039 1105 38-39 0.06043096201354814 1105 40-41 0.08814110315923784 1105 42-43 0.07152888271260593 1105 44-45 0.10618171730762072 1105 46-47 0.10592368236621752 1105 48-49 0.04419219166786803 1105 50-51 0.10761593112087553 1105 52-53 -0.009190964963444515 1105 54-55 0.04777642955561845 1105 56-57 -0.14255687908944026 1105 58-59 0.04386022974016157 1105 60-61 -0.008194106813682822 1105 62-63 0.14999856134009093 1105 64-65 -0.0012881947073211109 1105 66-67 0.19600440567848665 1105 68-69 0.21319444137564147 1105 70-71 -0.02687139207148448 1105 72-73 -0.13858287232568145 1105 74-75 -0.25465602556557343 1105 76-77 -0.2813667175357537 1105 78-79 -0.11557398188115187 1105 80-81 0.1245568330640694 1105 82-83 0.07375885036899277 1105 84-85 -0.03993006685714917 1105 86-87 -0.039312363424006236 1105 88-89 0.08888484674107033 1105 90-91 0.20735652015536132 1105 92-93 -0.004337718902686305 1105 94-95 0.05366699863428792 1105 96-97 0.15505737642125794 1105 98-99 0.33048179530559807 1105 100-101 0.5916004327592539 1106 1 -0.019847461690744694 1106 2 0.0899348554144801 1106 3 0.10175593082417578 1106 4 0.08375562628663857 1106 5 -0.0798231417055888 1106 6 0.005125760875579033 1106 7 0.023329254910848363 1106 8 0.0077058059458749995 1106 9 -0.04986523375762175 1106 10-11 0.04341409478085012 1106 12-13 0.005194769232197416 1106 14-15 0.15671823995329248 1106 16-17 0.07728495613314834 1106 18-19 -0.02587378825916886 1106 20-21 0.06172122139020786 1106 22-23 0.06666636415211968 1106 24-25 -0.1009566119603349 1106 26-27 -0.03441636301786133 1106 28-29 0.04754895691159078 1106 30-31 0.14140689498542258 1106 32-33 0.005057481249039597 1106 34-35 0.10210835123567819 1106 36-37 0.16456329941912884 1106 38-39 0.3295235896127906 1106 40-41 0.3849309932383562 1106 42-43 0.26504565089855703 1106 44-45 0.1731424398449093 1106 46-47 0.055447591909036475 1106 48-49 0.1192219473214422 1106 50-51 0.09053968663442902 1106 52-53 0.13788597965603344 1106 54-55 0.039920937012034585 1106 56-57 -0.006195470421793914 1106 58-59 0.038269667533342044 1106 60-61 0.025254748468817922 1106 62-63 0.023817086859935443 1106 64-65 0.2107155860110126 1106 66-67 0.25483308312720254 1106 68-69 0.19591165764673946 1106 70-71 0.321175236788001 1106 72-73 0.17095293947856405 1106 74-75 0.22420695840137483 1106 76-77 0.04719520923033116 1106 78-79 0.09909380499835763 1106 80-81 0.22409124460226337 1106 82-83 0.4250400938052472 1106 84-85 0.2526170170887987 1106 86-87 0.23511527782783048 1106 88-89 0.07907948372209717 1106 90-91 0.3867165985702634 1106 92-93 0.3906283246455864 1106 94-95 0.5415231913108975 1106 96-97 0.5041332008278907 1106 98-99 0.9075769433837308 1106 100-101 1.0161155906392558 1107 1 -0.005706305914323195 1107 2 0.05374656583548543 1107 3 0.029312564315397083 1107 4 -0.019853377419650542 1107 5 -0.04404349327298718 1107 6 0.007311777857630375 1107 7 -0.05320082756340838 1107 8 -0.09082319757771273 1107 9 -0.0275584615912976 1107 10-11 -0.023069972426718266 1107 12-13 0.00212701711882346 1107 14-15 0.13157960291260196 1107 16-17 -0.05150389383028653 1107 18-19 -0.07617547321877538 1107 20-21 0.03153536883902319 1107 22-23 -0.04577237393235123 1107 24-25 -0.014968659058617106 1107 26-27 0.025447078164866355 1107 28-29 0.03560325766679995 1107 30-31 -0.020046349047738943 1107 32-33 0.08151783285704539 1107 34-35 0.013178151629809065 1107 36-37 0.025556593786411952 1107 38-39 0.1991331119868036 1107 40-41 0.20866907517262945 1107 42-43 -0.05227445082527282 1107 44-45 0.07197970048761704 1107 46-47 0.10297430304308364 1107 48-49 0.039220292862751194 1107 50-51 0.12037064257152252 1107 52-53 0.007542953529288354 1107 54-55 0.19570532551953335 1107 56-57 -0.008813372172326694 1107 58-59 0.0853705331806367 1107 60-61 0.04090384691571103 1107 62-63 0.022848771773865906 1107 64-65 0.1156348093492312 1107 66-67 0.14139583354805296 1107 68-69 0.07640320442858695 1107 70-71 -0.044907700312627696 1107 72-73 0.06838127122087556 1107 74-75 -0.001563265706721495 1107 76-77 0.053362838375804245 1107 78-79 0.07070354762852915 1107 80-81 0.3281795845762687 1107 82-83 0.30499536252029813 1107 84-85 0.3545480647316275 1107 86-87 0.329207337796376 1107 88-89 0.326049210680587 1107 90-91 0.3139278764974982 1107 92-93 0.1952967401664374 1107 94-95 0.5827840207834036 1107 96-97 0.6376385775648856 1107 98-99 0.8879863762342808 1107 100-101 1.0405474794677474 1108 1 0.010359606195589066 1108 2 0.051294664660009204 1108 3 0.0010917190589552206 1108 4 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-1.457759858398198 2316 96-97 -1.2674191149892273 2316 98-99 -1.4858248481827943 2316 100-101 -1.6946743420427488 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 14.0 4 34.0 5 84.0 6 186.0 7 431.0 8 998.0 9 1884.0 10 3095.0 11 4930.0 12 7184.0 13 10062.0 14 13008.0 15 17061.0 16 21627.0 17 27685.0 18 24751.0 19 17438.0 20 16043.0 21 16923.0 22 18317.0 23 20686.0 24 24275.0 25 29514.0 26 36201.0 27 44730.0 28 55565.0 29 69142.0 30 85340.0 31 105462.0 32 131766.0 33 165840.0 34 218164.0 35 292554.0 36 412804.0 37 541720.0 38 330074.0 39 18841.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40472740689592 19.252544754373226 14.893023854029735 22.449703984701117 2 15.786710034170587 20.832528213899103 39.143458275897494 24.23730347603282 3 20.16553591759479 24.379536614361292 29.64712727481692 25.807800193227 4 13.19818101762166 16.87644150618407 36.858024704659584 33.06735277153469 5 13.64630859756588 37.842046590739315 33.76524915817177 14.746395653523036 6 32.03669266125226 36.1553153145388 16.985727040330755 14.822264983878187 7 28.872382055109185 32.90924984449274 20.07483741770326 18.14353068269481 8 26.835380665958926 34.14436532209755 19.241311346355243 19.778942665588293 9 26.360629667313244 17.090451736124702 20.334284947767753 36.2146336487943 10-11 25.412397005124042 26.5747744819617 26.650445469392665 21.362383043521593 12-13 26.65276901368085 23.93369422457884 27.316717443559714 22.096819318180593 14-15 23.024167046764617 25.40102428915697 26.336011377798208 25.238797286280203 16-17 22.072438018502776 28.762661443164788 26.817801280663023 22.347099257669413 18-19 21.96805744502657 27.33936064610571 29.177398941832383 21.515182967035333 20-21 23.007693479038487 25.945290177256723 29.251058383098048 21.795957960606742 22-23 22.445694148972944 26.134740750357842 29.271260884110582 22.148304216558625 24-25 22.728236695825967 26.216363027455476 29.341702528446255 21.713697748272303 26-27 22.41554119575508 26.247882783419698 29.531418129264065 21.80515789156116 28-29 21.87222045137221 27.026070404008205 29.329460409530267 21.772248735089317 30-31 22.183508633880408 26.27865128266919 29.290511790924356 22.247328292526042 32-33 22.048380475853396 26.442333419167806 29.335745317980816 22.173540786997982 34-35 22.81011017870817 26.34546534664845 29.042968834180254 21.80145564046313 36-37 22.741520690484997 25.999037401270776 29.25238763994497 22.007054268299253 38-39 22.36875588478738 26.42873164435708 29.278194405808648 21.92431806504689 40-41 22.50171312331471 26.48691561664538 29.02341555846383 21.987955701576073 42-43 22.371953860508416 26.589717234359973 28.52591521555969 22.512413689571925 44-45 22.619781514280046 26.377154082982194 28.535488441374557 22.467575961363206 46-47 22.984465430150102 26.8221739778447 28.081064919813176 22.112295672192026 48-49 23.025745416097728 26.891634086548788 27.80146515357967 22.281155343773815 50-51 22.487114385540746 27.112952558779646 28.138342736604088 22.261590319075523 52-53 23.039644642184697 27.360278355960112 27.371250402024028 22.228826599831166 54-55 22.58458077340177 26.947093839804865 27.71007660535162 22.758248781441747 56-57 22.93973830963431 27.120768496595765 27.487508518449683 22.451984675320244 58-59 23.435274157770255 26.83585155128942 27.332312290990245 22.39656199995008 60-61 22.898294112312577 26.98303270717974 28.11617886186162 22.002494318646065 62-63 22.721849392927755 27.10428066293977 27.893950468076856 22.27991947605562 64-65 22.510136420769978 27.472650026839496 27.61283676649569 22.404376785894836 66-67 22.734984687579573 27.234451527309332 27.22947728026129 22.801086504849803 68-69 22.625030079049537 27.112770397187113 27.58261412979065 22.679585393972697 70-71 22.994144233928964 27.016145106637435 27.418434978729977 22.571275680703625 72-73 22.88636841950264 26.844309837444452 27.71077954979202 22.558542193260887 74-75 22.535059761242362 27.68140818016799 27.280886818190638 22.502645240399016 76-77 22.746093101141653 27.199504370429235 27.241956120621154 22.81244640780796 78-79 22.826396141817238 27.21725910421087 27.268636174916928 22.687708579054963 80-81 22.98463982955731 27.391938810729815 27.17599421609688 22.447427143615993 82-83 22.869921737439263 27.311318916567885 27.375195360095596 22.443563985897256 84-85 22.671735422851388 27.453926942172686 27.1887025807521 22.685635054223827 86-87 22.75417337087691 27.3977667796805 27.07750905068383 22.770550798758762 88-89 22.689671744119096 27.421439805986697 26.787832791394393 23.10105565849981 90-91 22.82387871421203 27.06554441106365 27.09672216882253 23.013854705901792 92-93 23.077145470174763 27.17792784825288 27.17720955948966 22.56771712208269 94-95 22.699766804496115 27.062799732362098 27.226320312861958 23.01111315027983 96-97 22.40823659654091 27.052590277447315 27.501699197583683 23.037473928428092 98-99 22.422468530029406 27.33869406580635 26.837027770864065 23.401809633300182 100-101 22.180494064736767 27.329111791834976 27.011795821629274 23.47859832179898 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 357.0 1 259.0 2 220.5 3 301.5 4 290.0 5 289.5 6 446.0 7 915.0 8 1541.0 9 1935.5 10 1974.0 11 1872.5 12 1997.5 13 2282.0 14 2550.0 15 2840.5 16 3181.0 17 3490.0 18 3762.5 19 4015.5 20 4446.5 21 5080.5 22 5930.5 23 7147.5 24 8495.5 25 10316.0 26 13076.0 27 16508.5 28 20002.5 29 24483.5 30 30266.5 31 36370.0 32 43175.5 33 48833.5 34 53750.0 35 58439.5 36 64250.0 37 70451.5 38 74180.5 39 76556.5 40 79090.5 41 84156.5 42 88980.0 43 92899.5 44 97561.0 45 102423.5 46 104513.5 47 112479.5 48 133006.0 49 131457.5 50 111035.0 51 99950.0 52 93927.0 53 91661.5 54 89343.0 55 83612.0 56 76395.5 57 69354.0 58 63829.5 59 57217.5 60 47517.0 61 38517.5 62 31314.0 63 24262.5 64 18075.5 65 14000.0 66 10603.0 67 8209.0 68 6129.5 69 4151.5 70 3006.0 71 2281.5 72 1804.0 73 1519.0 74 1240.0 75 970.5 76 706.0 77 428.5 78 246.5 79 165.0 80 108.5 81 72.5 82 47.0 83 29.0 84 20.5 85 7.5 86 2.0 87 2.0 88 1.0 89 2.0 90 1.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.034656928728115856 2 0.00610536568267326 3 0.005243431703942917 4 7.901061471694806E-4 5 7.182783156086188E-4 6 3.591391578043094E-4 7 0.0 8 0.0 9 0.0016161262101193922 10-11 4.848378630358177E-4 12-13 0.001185159220754221 14-15 0.002370318441508442 16-17 0.003483649830701801 18-19 0.0 20-21 0.0 22-23 0.0015981692522291768 24-25 6.644074419379723E-4 26-27 0.004309669893651712 28-29 0.005333216493393994 30-31 9.87632683961851E-4 32-33 2.5139741046301654E-4 34-35 0.008332028461059977 36-37 0.010989658228811868 38-39 0.008780952408315366 40-41 0.001723867957460685 42-43 3.591391578043094E-4 44-45 0.0015622553364487458 46-47 3.591391578043094E-5 48-49 6.464504840477569E-4 50-51 0.0011672022628640055 52-53 0.0031424676307877067 54-55 0.0036093485359333095 56-57 0.001382685757546591 58-59 9.517187681814199E-4 60-61 0.0038966598621767564 62-63 0.006141279598453691 64-65 0.01050482036577605 66-67 0.0034118219991409395 68-69 0.0038068750727256796 70-71 3.591391578043094E-5 72-73 0.001221073136534652 74-75 0.006823643998281879 76-77 0.0035375207043724477 78-79 0.004309669893651712 80-81 0.005638484777527658 82-83 0.0055486999880765804 84-85 0.006805687040391663 86-87 0.004166014230529988 88-89 0.010666432986787988 90-91 0.014365566312172376 92-93 0.001454513589107453 94-95 0.002765371515093182 96-97 0.0012031161786444364 98-99 0.004130100314749558 100-101 0.005207517788162486 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2784436.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.42145886104605 #Duplication Level Percentage of deduplicated Percentage of total 1 75.75346420777973 40.468605717576295 2 13.769401139533702 14.711629930336805 3 4.5008527247894365 7.213263560308977 4 1.9697324131790637 4.2090391631165724 5 1.0550223439460218 2.818041637229839 6 0.6408070130421913 2.0539707291061933 7 0.42559107240622673 1.5914987176324398 8 0.30769034571892756 1.3149813716611782 9 0.22712322661745418 1.0919928696419132 >10 1.1944059044131394 11.748206151911806 >50 0.102849250688362 3.7698630198294913 >100 0.04793653536539058 4.690401173603453 >500 0.0032360984340038517 1.1744837937862385 >1k 0.0016180492169588037 1.851729830496503 >5k 2.0225615211985046E-4 0.8626927418579129 >10k+ 6.74187173732835E-5 0.42959959190419517 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11928 0.42838118742898024 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9759 0.35048390410122554 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8704 0.3125947229528709 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5490 0.19716739763456587 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4975 0.1786717310076439 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4454 0.1599605808860394 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3855 0.13844814533356126 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3480 0.12498042691589967 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3125 0.11223098681384669 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2839 0.10195960690064343 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.591391578043094E-5 0.0 3 0.0 0.0 0.0 3.591391578043094E-5 0.0 4 0.0 0.0 0.0 3.591391578043094E-5 0.0 5 0.0 0.0 0.0 3.591391578043094E-5 0.0 6 0.0 0.0 0.0 3.591391578043094E-5 0.0 7 0.0 0.0 0.0 3.591391578043094E-5 0.0 8 0.0 0.0 0.0 3.591391578043094E-5 0.0 9 0.0 0.0 0.0 3.591391578043094E-5 0.0 10-11 0.0 0.0 0.0 3.591391578043094E-5 0.0 12-13 0.0 0.0 0.0 3.591391578043094E-5 0.0 14-15 0.0 0.0 0.0 3.591391578043094E-5 0.0 16-17 1.795695789021547E-5 0.0 0.0 3.591391578043094E-5 0.0 18-19 3.591391578043094E-5 0.0 0.0 3.591391578043094E-5 0.0 20-21 3.591391578043094E-5 0.0 0.0 5.3870873670646405E-5 0.0 22-23 3.591391578043094E-5 0.0 0.0 7.182783156086188E-5 0.0 24-25 3.591391578043094E-5 0.0 0.0 1.0774174734129281E-4 0.0 26-27 3.591391578043094E-5 0.0 0.0 1.2569870523150827E-4 0.0 28-29 3.591391578043094E-5 0.0 0.0 8.080631050596961E-4 0.0 30-31 3.591391578043094E-5 0.0 0.0 0.002154834946825856 0.0 32-33 3.591391578043094E-5 0.0 0.0 0.003932573777957188 0.0 34-35 3.591391578043094E-5 0.0 0.0 0.00535117345128421 0.0 36-37 3.591391578043094E-5 0.0 0.0 0.0073084818613176955 0.0 38-39 3.591391578043094E-5 0.0 0.0 0.010881916481470574 0.0 40-41 3.591391578043094E-5 0.0 0.0 0.015425026827695087 0.0 42-43 3.591391578043094E-5 0.0 0.0 0.020309319373833696 0.0 44-45 3.591391578043094E-5 0.0 0.0 0.023200389594158384 0.0 46-47 3.591391578043094E-5 0.0 0.0 0.026648125509079756 0.0 48-49 3.591391578043094E-5 0.0 0.0 0.03187360025513246 0.0 50-51 3.591391578043094E-5 0.0 0.0 0.03765574069578184 0.0 52-53 3.591391578043094E-5 0.0 0.0 0.049363677240202325 0.0 54-55 3.591391578043094E-5 0.0 0.0 0.059078391458808895 0.0 56-57 3.591391578043094E-5 0.0 0.0 0.07958523736943496 0.0 58-59 3.591391578043094E-5 0.0 0.0 0.11241055639274883 0.0 60-61 3.591391578043094E-5 0.0 0.0 0.13995652979633938 0.0 62-63 3.591391578043094E-5 0.0 0.0 0.15478897701365735 0.0 64-65 3.591391578043094E-5 0.0 0.0 0.18127548990172515 0.0 66-67 3.591391578043094E-5 0.0 0.0 0.24150312666550786 0.0 68-69 3.591391578043094E-5 0.0 0.0 0.3301566277695016 0.0 70-71 3.591391578043094E-5 0.0 0.0 0.4029182211406547 0.0 72-73 3.591391578043094E-5 0.0 0.0 0.48817785720339774 0.0 74-75 3.591391578043094E-5 0.0 0.0 0.5872284369258263 3.591391578043094E-5 76-77 3.591391578043094E-5 0.0 0.0 0.6480666102578763 3.591391578043094E-5 78-79 3.591391578043094E-5 0.0 0.0 0.7348346307833975 3.591391578043094E-5 80-81 3.591391578043094E-5 0.0 0.0 0.8527938871642229 3.591391578043094E-5 82-83 3.591391578043094E-5 0.0 0.0 0.9600867105582602 3.591391578043094E-5 84-85 3.591391578043094E-5 1.795695789021547E-5 0.0 1.1115716073201178 3.591391578043094E-5 86-87 3.591391578043094E-5 3.591391578043094E-5 0.0 1.3286891851707132 3.591391578043094E-5 88-89 3.591391578043094E-5 3.591391578043094E-5 0.0 1.6249610334013782 3.591391578043094E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13000 0.0 23.025122 1 TACATGG 13380 0.0 22.115473 2 ACATGGG 13100 0.0 21.862637 3 GAGTACT 11100 0.0 21.69721 12-13 GTACTTT 11745 0.0 20.343884 14-15 GTACCTG 2420 0.0 19.63312 1 AGTACTT 11540 0.0 19.614445 12-13 GTATCAA 27965 0.0 18.230124 1 AGAGTAC 19890 0.0 18.163149 10-11 GTGTAGC 1455 0.0 17.95992 1 CATGGGA 7515 0.0 17.885067 4 TACCTGG 2470 0.0 17.883408 2 ATAACGC 215 2.3981705E-5 17.672909 3 ACTTTTT 13785 0.0 17.554878 16-17 CGTAACG 110 2.9189878E-5 17.273731 14-15 TACTTTT 13035 0.0 17.255512 14-15 CATGGGG 5875 0.0 16.89552 4 GGACCGT 340 1.7558705E-8 16.762068 6 GTACCGT 260 6.639577E-6 16.439724 6 TAACGCA 290 1.03204E-6 16.37703 4 >>END_MODULE