FastQCFastQC Report
Thu 26 May 2016
SRR936576_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936576_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819214
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT101030.3583622953064223No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83510.2962173144713385No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67790.24045709194122902No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT65110.23095089624271165No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT48260.17118246433225714No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38810.13766248323114175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37700.13372521560974088No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32220.11428717365904115No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT28350.10055994330334624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG128750.024.3274351
TACATGG133550.023.1315352
GAGTACT90800.022.69321612-13
ACATGGG131450.022.3862173
GTACTTT97350.020.96694814-15
GTACCTG25900.020.7365651
TACCTGG26200.020.4980322
AGTACTT95750.020.20451712-13
GTATCAA252200.019.8822481
AGAGTAC175050.019.29569210-11
CATGGGA78200.018.4806614
ACTTTTT118450.017.89242716-17
ATGGGAG36450.017.7377995
TATCAAC283350.017.6452392
ATCAACG281350.017.6233333
TCAACGC280600.017.6196124
CAACGCA280700.017.5289675
AACGCAG283450.017.4095276
TAAGGTG11200.016.978545
GTGTAGC14850.016.963191
Produced by FastQC (version 0.11.5)