##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936576_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2819214 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.143167918434003 31.0 31.0 34.0 28.0 34.0 2 31.197270232057587 31.0 31.0 34.0 28.0 34.0 3 31.143718071774614 31.0 31.0 34.0 27.0 34.0 4 34.81390486852009 37.0 35.0 37.0 32.0 37.0 5 34.85139262219895 37.0 35.0 37.0 32.0 37.0 6 34.904263386887266 37.0 35.0 37.0 32.0 37.0 7 34.76185667352674 37.0 35.0 37.0 32.0 37.0 8 34.742208289260766 37.0 35.0 37.0 32.0 37.0 9 36.18843124360194 39.0 35.0 39.0 32.0 39.0 10-11 36.05724042233048 39.0 35.0 39.0 30.0 39.0 12-13 35.94303571846621 38.5 35.0 39.0 30.0 39.0 14-15 36.87484916008505 39.0 36.0 41.0 30.5 41.0 16-17 36.79023621477475 39.0 36.0 41.0 30.0 41.0 18-19 36.67081037480659 39.0 36.0 41.0 30.0 41.0 20-21 36.64087383930415 39.0 36.0 41.0 30.0 41.0 22-23 36.63309241511996 39.0 36.0 40.5 30.0 41.0 24-25 36.74247378879362 39.0 36.0 41.0 30.0 41.0 26-27 36.66182542368192 39.0 36.0 41.0 30.0 41.0 28-29 36.54875454647998 39.0 36.0 41.0 30.0 41.0 30-31 36.36031603134774 39.0 35.5 40.5 30.0 41.0 32-33 36.19453400841511 39.0 35.0 40.0 29.5 41.0 34-35 35.98632934569706 38.0 35.0 40.0 29.0 41.0 36-37 35.77072971402667 38.0 35.0 40.0 27.5 41.0 38-39 35.57707963992801 38.0 35.0 40.0 27.0 41.0 40-41 35.35559627612519 38.0 34.0 40.0 26.5 41.0 42-43 35.075134062188965 38.0 34.0 40.0 25.5 41.0 44-45 34.92714405504513 38.0 34.0 40.0 25.0 41.0 46-47 34.77791966129567 38.0 34.0 40.0 24.0 41.0 48-49 34.53530061925061 38.0 33.0 40.0 24.0 41.0 50-51 33.5569367916022 37.0 32.0 39.5 22.5 40.5 52-53 33.67428297390691 37.5 33.0 39.5 23.0 40.5 54-55 34.118487812560524 38.0 33.0 40.0 23.0 41.0 56-57 33.9605932717417 38.0 33.0 40.0 22.0 41.0 58-59 33.73086523406879 37.5 33.0 40.0 22.0 41.0 60-61 33.422607152206254 37.0 32.5 40.0 20.5 41.0 62-63 33.089260694647514 37.0 32.0 40.0 19.5 41.0 64-65 32.72780853103028 36.0 31.5 39.0 18.0 41.0 66-67 32.35098133735147 36.0 31.0 39.0 17.5 41.0 68-69 31.900799655506816 35.0 31.0 39.0 13.5 40.0 70-71 31.485662493162987 35.0 31.0 38.0 12.0 40.0 72-73 31.060706281963697 35.0 30.0 37.0 8.0 39.5 74-75 30.645686882939714 35.0 30.0 37.0 7.0 39.0 76-77 30.175547688114488 34.0 29.5 36.0 6.0 39.0 78-79 29.699811543217365 34.0 29.0 36.0 2.0 38.5 80-81 29.236173273827383 34.0 29.0 35.0 2.0 37.0 82-83 28.819930661524808 34.0 28.5 35.0 2.0 37.0 84-85 28.439268001648685 34.0 27.5 35.0 2.0 36.0 86-87 28.085919337801244 34.0 27.0 35.0 2.0 36.0 88-89 27.720573358389963 33.5 26.5 35.0 2.0 35.5 90-91 27.337648188466716 33.0 26.0 35.0 2.0 35.0 92-93 26.889876752882188 33.0 25.0 35.0 2.0 35.0 94-95 26.369803604834537 33.0 25.0 35.0 2.0 35.0 96-97 25.74778679447534 32.5 23.5 35.0 2.0 35.0 98-99 24.972644325687938 32.0 17.0 35.0 2.0 35.0 100-101 22.35459227288173 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5822257790151539 1101 2 0.4862955702795162 1101 3 0.6386869833042219 1101 4 0.6590712449568414 1101 5 0.6689829387131567 1101 6 0.7043319455525463 1101 7 0.728114037207888 1101 8 0.6814930702679405 1101 9 0.899259261802527 1101 10-11 0.8945760073979088 1101 12-13 0.9135096800185849 1101 14-15 1.1232956610954545 1101 16-17 1.1799835280218147 1101 18-19 1.3621430839119526 1101 20-21 1.2094933831273096 1101 22-23 1.1083486539003786 1101 24-25 0.9851075616076415 1101 26-27 0.97529873870171 1101 28-29 0.9533748547351166 1101 30-31 0.7984608379683777 1101 32-33 0.8193264020202591 1101 34-35 0.8925642281694834 1101 36-37 0.9457671046176515 1101 38-39 0.9101259678497087 1101 40-41 0.9086834410623936 1101 42-43 0.9018085152317994 1101 44-45 0.9225543482668428 1101 46-47 1.055850648170349 1101 48-49 1.0042032543038104 1101 50-51 0.9202287358659902 1101 52-53 0.956386949766916 1101 54-55 0.9159298435008907 1101 56-57 1.1395577343627963 1101 58-59 1.1262857771335746 1101 60-61 1.0068278902500722 1101 62-63 1.158117639646818 1101 64-65 1.1493437444407206 1101 66-67 1.0818117854367344 1101 68-69 1.105506124136415 1101 70-71 0.9012177248390998 1101 72-73 1.0033900483113136 1101 74-75 0.9623548663722161 1101 76-77 1.029768905422351 1101 78-79 0.9709491563163617 1101 80-81 1.033754400696349 1101 82-83 1.1632724475559861 1101 84-85 1.13739627810887 1101 86-87 1.0418223027108198 1101 88-89 1.2430902720533403 1101 90-91 1.197404603298633 1101 92-93 1.3113445733312865 1101 94-95 1.4142437465818567 1101 96-97 1.3240494787329595 1101 98-99 1.1623929136187385 1101 100-101 1.5699734011043596 1102 1 0.38099281743405555 1102 2 0.49195618938362884 1102 3 0.5389991059735415 1102 4 0.42427029715487663 1102 5 0.5131239283098807 1102 6 0.445643558153904 1102 7 0.507358406795511 1102 8 0.4254028668975991 1102 9 0.6109149634946718 1102 10-11 0.524953717184637 1102 12-13 0.5132830079241657 1102 14-15 0.6979323289001016 1102 16-17 0.4163792491273597 1102 18-19 0.5635986427201871 1102 20-21 0.6830664497548398 1102 22-23 0.3903121357913406 1102 24-25 0.5439595924322802 1102 26-27 0.4500672010941287 1102 28-29 0.26178523887548977 1102 30-31 0.36181427938637967 1102 32-33 0.43082429890218776 1102 34-35 0.13255363756917404 1102 36-37 0.28344395588460003 1102 38-39 0.07747813125958913 1102 40-41 0.31670628707944104 1102 42-43 0.234293255766147 1102 44-45 0.35358275962003916 1102 46-47 0.35227484063706527 1102 48-49 0.4986802886762334 1102 50-51 0.46065243286128776 1102 52-53 0.3164821597503007 1102 54-55 0.5120835337088039 1102 56-57 0.34831744207520643 1102 58-59 0.2005568218971021 1102 60-61 0.2843564568942156 1102 62-63 0.2721170184829589 1102 64-65 0.28104730656399113 1102 66-67 0.29227776779600134 1102 68-69 0.2642198730224088 1102 70-71 -0.10170077213901152 1102 72-73 0.25954796340648656 1102 74-75 0.18194105042322306 1102 76-77 0.2285905997724349 1102 78-79 0.13062110720062847 1102 80-81 0.31668682926047964 1102 82-83 0.19342666810483422 1102 84-85 0.3261830542928301 1102 86-87 0.19377075827530632 1102 88-89 0.2699740568387199 1102 90-91 0.5796227948036083 1102 92-93 0.5245236874673012 1102 94-95 0.6974992249469487 1102 96-97 0.6072568087907193 1102 98-99 0.6314622382611859 1102 100-101 1.1873104206045255 1103 1 0.3258729944093126 1103 2 0.41713106290178104 1103 3 0.43186876391831674 1103 4 0.41302519293796536 1103 5 0.38047949426598393 1103 6 0.34824359740757416 1103 7 0.47050922115027305 1103 8 0.20341501010834406 1103 9 0.3084183425683307 1103 10-11 0.23610966630107555 1103 12-13 0.17625596897800477 1103 14-15 0.4678467137815616 1103 16-17 0.700938273061773 1103 18-19 0.7346524766676268 1103 20-21 0.8406158088631557 1103 22-23 0.6018889295609711 1103 24-25 0.40268948187836884 1103 26-27 0.43650521644755713 1103 28-29 0.45617504293291944 1103 30-31 0.43072533167878646 1103 32-33 0.48374226111218377 1103 34-35 0.37445895834874676 1103 36-37 0.4393055660320684 1103 38-39 0.29578814150210064 1103 40-41 0.48120410230271915 1103 42-43 0.5658072992836836 1103 44-45 0.5863187921862831 1103 46-47 0.7322963705608458 1103 48-49 0.6958835042988696 1103 50-51 0.6717156114074925 1103 52-53 0.7767545039493697 1103 54-55 0.7300455053237869 1103 56-57 0.6757737167428388 1103 58-59 0.5534411721574628 1103 60-61 0.6260885728339858 1103 62-63 0.6610855448254398 1103 64-65 0.6849888440206584 1103 66-67 0.6918158816014497 1103 68-69 0.8404889029084437 1103 70-71 0.6484235841285795 1103 72-73 0.689053653202361 1103 74-75 0.5926825514868064 1103 76-77 0.4246982485966946 1103 78-79 0.39393259144234705 1103 80-81 0.6854069370729938 1103 82-83 0.515328230385073 1103 84-85 0.4950505222959265 1103 86-87 0.5212032940068738 1103 88-89 0.3736812244479246 1103 90-91 0.5394075472154434 1103 92-93 0.5959271293945143 1103 94-95 0.6061322317225795 1103 96-97 0.7765291299461445 1103 98-99 1.010476303630469 1103 100-101 1.4409615484324405 1104 1 0.21941379688819396 1104 2 0.3100005148914349 1104 3 0.4181588451929876 1104 4 0.5678346352228942 1104 5 0.4310787388971704 1104 6 0.40672468124589756 1104 7 0.23482296697144278 1104 8 0.36715841383493597 1104 9 0.6035396487571631 1104 10-11 0.542134762673605 1104 12-13 0.45256727716098766 1104 14-15 0.4968258998775923 1104 16-17 0.5900839385453409 1104 18-19 0.637611117788218 1104 20-21 0.7230985513206818 1104 22-23 0.5273273267701768 1104 24-25 0.6748580769665509 1104 26-27 0.319806686117758 1104 28-29 0.14638397613709486 1104 30-31 0.30665471279169765 1104 32-33 0.41166392698566057 1104 34-35 0.34599233839991683 1104 36-37 0.3935162102819376 1104 38-39 0.2606871903882535 1104 40-41 0.3467073083775105 1104 42-43 0.4404266974390936 1104 44-45 0.6809061053222081 1104 46-47 0.6052385089835042 1104 48-49 0.620252837746385 1104 50-51 0.5610372454755534 1104 52-53 0.34981758254621553 1104 54-55 0.5298900510355224 1104 56-57 0.4691427641746486 1104 58-59 0.47863656281936784 1104 60-61 0.5271496172825394 1104 62-63 0.4571639259416429 1104 64-65 0.3913082472439555 1104 66-67 0.34455714690925987 1104 68-69 0.2814121957510416 1104 70-71 0.27773811537642956 1104 72-73 0.47949885517740043 1104 74-75 0.5460556338175806 1104 76-77 0.5632207668474258 1104 78-79 0.3350211248536574 1104 80-81 0.5261660961285948 1104 82-83 0.4727615511554646 1104 84-85 0.3903293604158975 1104 86-87 0.3953032814439297 1104 88-89 0.519299961058536 1104 90-91 0.531525465293015 1104 92-93 0.481119642423959 1104 94-95 0.6454744868815858 1104 96-97 0.594660962017457 1104 98-99 0.5729488889385834 1104 100-101 1.1406726881127582 1105 1 0.35021449610167465 1105 2 0.32533716008664726 1105 3 0.30705000467084176 1105 4 0.4417687855844932 1105 5 0.3700221858814743 1105 6 0.28591454149914597 1105 7 0.3967609242472747 1105 8 0.264145789999894 1105 9 0.2720868427889016 1105 10-11 0.5431956234123092 1105 12-13 0.589067650369067 1105 14-15 0.768646339783416 1105 16-17 0.7186246182253697 1105 18-19 0.6117943773434007 1105 20-21 0.4703513945138056 1105 22-23 0.5116939859534924 1105 24-25 0.168196936465705 1105 26-27 0.28763384760652144 1105 28-29 0.34708040734687273 1105 30-31 0.12703854146519689 1105 32-33 0.25319416643365855 1105 34-35 0.020935157934154347 1105 36-37 0.04224816629519523 1105 38-39 -0.09091218369671594 1105 40-41 0.03396292424758229 1105 42-43 -0.13570069795232342 1105 44-45 -0.07040448822253609 1105 46-47 0.21657429647173387 1105 48-49 0.3837913203917296 1105 50-51 0.43142140994039124 1105 52-53 0.45976803893459817 1105 54-55 0.28491099482022264 1105 56-57 0.2867937791279189 1105 58-59 0.39260596167614636 1105 60-61 0.1569915363203691 1105 62-63 0.34944758518444274 1105 64-65 0.4273577006716138 1105 66-67 0.32684463417317744 1105 68-69 0.3543946655250245 1105 70-71 0.5254758892142561 1105 72-73 0.3640593139259174 1105 74-75 0.31389152588081615 1105 76-77 0.3553989876139134 1105 78-79 0.35577638670373446 1105 80-81 0.3815414436726172 1105 82-83 0.3809919404623301 1105 84-85 0.4729537902901946 1105 86-87 0.49245706619593577 1105 88-89 0.26722712120413306 1105 90-91 0.3869030674359415 1105 92-93 0.4111614052708532 1105 94-95 0.522294475867632 1105 96-97 0.691366081587951 1105 98-99 1.013161243373041 1105 100-101 1.3751917485878167 1106 1 0.2917314341788 1106 2 0.15065555854096147 1106 3 0.2169159257621729 1106 4 0.3443506420826594 1106 5 0.3237077977187468 1106 6 0.28953110776244273 1106 7 0.4077912711891116 1106 8 0.46024252392025744 1106 9 0.4348652405810043 1106 10-11 0.5605614617268557 1106 12-13 0.5310262756122839 1106 14-15 0.3362844650159147 1106 16-17 0.3156801507332716 1106 18-19 0.5231877248169567 1106 20-21 0.23775185299978574 1106 22-23 0.16476135261957836 1106 24-25 0.05669369064204233 1106 26-27 -0.0754607574898003 1106 28-29 0.09929454016758399 1106 30-31 0.16746345902926407 1106 32-33 0.34159611684939506 1106 34-35 0.3024356672548194 1106 36-37 0.23121217105534697 1106 38-39 0.20994845136675622 1106 40-41 0.20479905738381632 1106 42-43 0.14573261609888988 1106 44-45 0.1406746970202093 1106 46-47 0.34168658074082714 1106 48-49 0.346813033228365 1106 50-51 0.280921340797434 1106 52-53 0.2101821176095413 1106 54-55 0.4153425637421151 1106 56-57 0.3559068405249164 1106 58-59 0.35210990477246895 1106 60-61 0.46101911364849 1106 62-63 0.3939846584701385 1106 64-65 0.26510795435810763 1106 66-67 0.3225947768413917 1106 68-69 0.32473520920292387 1106 70-71 0.09021575587546948 1106 72-73 0.1941090871175355 1106 74-75 0.3937714119020619 1106 76-77 0.3058851794729627 1106 78-79 0.3980582796236938 1106 80-81 0.45807013605267244 1106 82-83 0.58762494132516 1106 84-85 0.8291342943131461 1106 86-87 0.8714084401931146 1106 88-89 1.0025165672619174 1106 90-91 1.1104209735144828 1106 92-93 1.1480584738041877 1106 94-95 1.2209784711230611 1106 96-97 1.0372437978836793 1106 98-99 1.2777562506671387 1106 100-101 2.0234824567480487 1107 1 0.1686855369075495 1107 2 0.2386359397996145 1107 3 0.30201656133582944 1107 4 0.2963774966394652 1107 5 0.22035296106601976 1107 6 0.2429251270751891 1107 7 0.23110031729984115 1107 8 0.2925129389026324 1107 9 0.44315056033231315 1107 10-11 0.42711480875808405 1107 12-13 0.355002330934191 1107 14-15 0.09253502758016197 1107 16-17 0.2670361079459198 1107 18-19 0.5096994999304556 1107 20-21 0.3440495688966365 1107 22-23 0.3369215623610131 1107 24-25 0.27361564239924974 1107 26-27 0.1902242139236705 1107 28-29 0.2556372417629049 1107 30-31 0.13509815416173154 1107 32-33 0.22334112842339238 1107 34-35 0.350988047307375 1107 36-37 0.30710590216023803 1107 38-39 0.32117641707419864 1107 40-41 0.32550264614997815 1107 42-43 0.09002130841674472 1107 44-45 0.24073395216668558 1107 46-47 0.29016089680365553 1107 48-49 0.409010232722828 1107 50-51 0.39527026223906603 1107 52-53 0.3669293794404993 1107 54-55 0.4155313811995214 1107 56-57 0.5386095770123447 1107 58-59 0.5777083710595292 1107 60-61 0.5766737187434643 1107 62-63 0.5189356922955284 1107 64-65 0.538300910538652 1107 66-67 0.573662992773528 1107 68-69 0.5118202603620112 1107 70-71 0.5438274620329295 1107 72-73 0.5834544266166724 1107 74-75 0.508105655641728 1107 76-77 0.5539660456401201 1107 78-79 0.6753469950759303 1107 80-81 0.7631905447265517 1107 82-83 0.6877613759111121 1107 84-85 0.7083530886925367 1107 86-87 0.7824461109526268 1107 88-89 0.7154649443260546 1107 90-91 0.7527332014788293 1107 92-93 0.7974753413415208 1107 94-95 0.8061262503089885 1107 96-97 1.0335453085983026 1107 98-99 1.3614927347879018 1107 100-101 1.8261357478484825 1108 1 0.1714392472986752 1108 2 0.14275082874915768 1108 3 0.38284114230286903 1108 4 0.41407261496510017 1108 5 0.2825934721345362 1108 6 0.25966442713617255 1108 7 0.40284300128217154 1108 8 0.4035593366752366 1108 9 0.01352131026407477 1108 10-11 0.20305142441057455 1108 12-13 0.12109868593848461 1108 14-15 0.5310735286907189 1108 16-17 0.5881580681242227 1108 18-19 0.48302090751997895 1108 20-21 0.5401112830988595 1108 22-23 0.6754208465119902 1108 24-25 0.6195159227523135 1108 26-27 0.5773869252411856 1108 28-29 0.192867783707797 1108 30-31 0.44397518946105663 1108 32-33 0.30357461392326 1108 34-35 0.3327544441058876 1108 36-37 0.3231470084336294 1108 38-39 0.30043308065965135 1108 40-41 0.4481375793796829 1108 42-43 0.4958286653763082 1108 44-45 0.26923651369875756 1108 46-47 0.454304166047109 1108 48-49 0.351206768033812 1108 50-51 0.3475094846778646 1108 52-53 0.383395429120732 1108 54-55 0.3895795455109692 1108 56-57 0.47318051912242964 1108 58-59 0.5706386135755892 1108 60-61 0.4517199115529067 1108 62-63 0.4723158593709371 1108 64-65 0.4265142102120265 1108 66-67 0.41326339580007954 1108 68-69 0.45717317748485087 1108 70-71 0.38208479834397835 1108 72-73 0.5386460823841013 1108 74-75 0.5256078853770099 1108 76-77 0.7003657461098136 1108 78-79 0.6938429916991105 1108 80-81 0.8371060917904565 1108 82-83 0.8036431791403977 1108 84-85 0.8822176993690896 1108 86-87 0.927276016902951 1108 88-89 0.7591523329027048 1108 90-91 0.8310535284457892 1108 92-93 1.0412462371826976 1108 94-95 1.1450814252734531 1108 96-97 1.3827146497635319 1108 98-99 1.5829719599460148 1108 100-101 1.8986788535272794 1109 1 0.17763764791064673 1109 2 0.09643553769596735 1109 3 0.212453279058618 1109 4 0.19623349534905543 1109 5 0.16963329322324938 1109 6 0.23549450042023778 1109 7 0.32227137798621897 1109 8 0.3224061866847947 1109 9 0.38571042183495763 1109 10-11 0.32513123561755464 1109 12-13 0.28703462481913533 1109 14-15 0.06423739905587666 1109 16-17 -0.09862230342658762 1109 18-19 0.21671310360942897 1109 20-21 -0.1154376268501025 1109 22-23 0.07524092754866274 1109 24-25 -0.14472749931612583 1109 26-27 0.0340452000236624 1109 28-29 -0.12918932769623126 1109 30-31 0.12038722284871994 1109 32-33 0.2276989785048542 1109 34-35 0.08058022013846511 1109 36-37 0.11210471907994446 1109 38-39 0.2569214590648272 1109 40-41 0.07901304956086364 1109 42-43 0.16121206179775527 1109 44-45 0.13519580249605667 1109 46-47 0.2023580236321294 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0.42360262953516425 2311 76-77 0.655865282659807 2311 78-79 0.6534330985903516 2311 80-81 0.7833973789905144 2311 82-83 0.7764984221403779 2311 84-85 0.6338523455801983 2311 86-87 0.6953650329874641 2311 88-89 0.623952989251368 2311 90-91 0.7320679456205674 2311 92-93 1.0899786217216914 2311 94-95 1.0906816576901726 2311 96-97 0.9718252889034922 2311 98-99 1.2148653802792282 2311 100-101 0.9271073760630983 2312 1 0.16293107014547914 2312 2 0.2470762905214272 2312 3 0.19008367580520158 2312 4 0.33663638937657936 2312 5 0.21954817476672872 2312 6 0.17682660426475394 2312 7 0.07055955124956625 2312 8 0.17022620843729186 2312 9 0.4188995266725257 2312 10-11 0.1532806185087523 2312 12-13 -0.034551179313844216 2312 14-15 0.13522855619400076 2312 16-17 0.18684135572127758 2312 18-19 0.08419548881773409 2312 20-21 0.04245829219278363 2312 22-23 0.10191137725783506 2312 24-25 0.021253490895254856 2312 26-27 0.07894636803803223 2312 28-29 -0.08082904018499448 2312 30-31 0.03166423613666325 2312 32-33 0.18767438872852438 2312 34-35 -0.00778337753495606 2312 36-37 0.21008079133606117 2312 38-39 0.17614404382862148 2312 40-41 0.32268472875886545 2312 42-43 0.06415219515000814 2312 44-45 -0.11346594621534933 2312 46-47 0.07073415263387517 2312 48-49 0.15957589051267718 2312 50-51 0.12347763746572582 2312 52-53 -0.025030914971914342 2312 54-55 -0.1937585050163264 2312 56-57 -0.017112726265999356 2312 58-59 0.22420942686084544 2312 60-61 -0.04608136332003454 2312 62-63 0.04277873915633279 2312 64-65 0.08101902790273385 2312 66-67 0.034289877716823014 2312 68-69 0.04870653214810261 2312 70-71 0.018382067703953453 2312 72-73 -0.17042908097437603 2312 74-75 -0.3764893175320623 2312 76-77 -0.29073537001930205 2312 78-79 -0.1810330281377155 2312 80-81 -0.44853374429730763 2312 82-83 -0.41677693825620565 2312 84-85 -0.6247251897686503 2312 86-87 -0.861653754333652 2312 88-89 -0.5158882759844907 2312 90-91 -0.5983780701907371 2312 92-93 -0.38798047115935574 2312 94-95 -0.5722625689182443 2312 96-97 -0.5797682760452645 2312 98-99 -0.29471714825820783 2312 100-101 -0.5524643481758318 2313 1 -0.0755130117749907 2313 2 -0.14668201667193514 2313 3 0.0386462340429965 2313 4 0.0293022744092184 2313 5 -0.1286785979708469 2313 6 0.0848534167369479 2313 7 0.0828083067445533 2313 8 0.15602514007975543 2313 9 0.23481617911494368 2313 10-11 0.12504560503536055 2313 12-13 0.10562800422371765 2313 14-15 0.12015388589339437 2313 16-17 0.2893340034179275 2313 18-19 -0.013354256405015974 2313 20-21 0.016883119613034125 2313 22-23 -0.04206225238754513 2313 24-25 -0.17307817003973724 2313 26-27 -0.23365545191937542 2313 28-29 -0.08787291056059843 2313 30-31 -0.08395671519961923 2313 32-33 -0.12163246605263822 2313 34-35 -0.24228163005124514 2313 36-37 -0.09456815891451953 2313 38-39 -0.06591784703549308 2313 40-41 0.14376275411625983 2313 42-43 0.2832293586579553 2313 44-45 0.2935714183161906 2313 46-47 0.18487421995887132 2313 48-49 0.010490206447663297 2313 50-51 0.19400546428995114 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2315 92-93 -1.9056466839353874 2315 94-95 -2.1744960068437713 2315 96-97 -1.8458863270783574 2315 98-99 -2.046407397503369 2315 100-101 -2.269088585935588 2316 1 -5.479356681217876 2316 2 -5.417861217877981 2316 3 -5.97138761766837 2316 4 -7.653140734524424 2316 5 -8.49175159394008 2316 6 -9.311127405878196 2316 7 -11.44012343561553 2316 8 -11.221005516217716 2316 9 -11.574698886104478 2316 10-11 -11.260777516009107 2316 12-13 -9.685342492026479 2316 14-15 -9.66261801224968 2316 16-17 -8.620114769304358 2316 18-19 -9.078019550190888 2316 20-21 -10.54807442483025 2316 22-23 -9.965922247254994 2316 24-25 -10.160850994212147 2316 26-27 -8.03594993905142 2316 28-29 -6.449310038070113 2316 30-31 -8.496067428396259 2316 32-33 -6.214377542604986 2316 34-35 -4.661894843877199 2316 36-37 -5.362939600659928 2316 38-39 -4.432886293067238 2316 40-41 -3.9588102245227432 2316 42-43 -4.050488769915528 2316 44-45 -4.300999809094559 2316 46-47 -4.016576197559573 2316 48-49 -4.934231181258937 2316 50-51 -4.755610487361867 2316 52-53 -4.505559494180986 2316 54-55 -4.1587402208298485 2316 56-57 -4.706024799487629 2316 58-59 -4.960135313164194 2316 60-61 -5.252135480268095 2316 62-63 -4.834300070284961 2316 64-65 -5.349034410019442 2316 66-67 -4.744981265805201 2316 68-69 -4.995898218203788 2316 70-71 -4.583584808564794 2316 72-73 -3.6943355663788573 2316 74-75 -4.227975217135256 2316 76-77 -4.485193528585992 2316 78-79 -4.52903485301977 2316 80-81 -5.052042752000009 2316 82-83 -4.344250254661045 2316 84-85 -4.446735884426271 2316 86-87 -5.062034645413654 2316 88-89 -6.671127208216291 2316 90-91 -5.842071970982229 2316 92-93 -5.2712736052174485 2316 94-95 -4.719049750890111 2316 96-97 -6.12432722751613 2316 98-99 -7.788957452688145 2316 100-101 -8.4991735482692 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7824.0 3 3150.0 4 3238.0 5 3587.0 6 3861.0 7 4682.0 8 5728.0 9 7273.0 10 8996.0 11 11130.0 12 13531.0 13 16137.0 14 18040.0 15 20922.0 16 24463.0 17 28915.0 18 24480.0 19 20531.0 20 21119.0 21 23132.0 22 25296.0 23 28533.0 24 32936.0 25 37856.0 26 43826.0 27 51375.0 28 60742.0 29 72326.0 30 86226.0 31 104143.0 32 127776.0 33 160096.0 34 205431.0 35 272685.0 36 368885.0 37 468441.0 38 353091.0 39 48800.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.86799532148796 18.226531564869983 14.207341856176575 22.698131257465477 2 15.46526290989525 19.92976721774717 39.95522899633898 24.649740876018594 3 20.00596714381349 24.23884326810746 29.300982095375677 26.454207492703375 4 12.788545048931677 16.185628164664745 37.319521865988506 33.70630492041507 5 13.778897998964695 37.46164170827581 33.794679493388486 14.964780799371011 6 31.94758653968975 35.56557714415476 17.466469861905505 15.02036645424999 7 28.931337137583093 32.64827255704156 20.289915779945154 18.130474525430195 8 26.648289539482256 33.75663189634704 19.63229419871218 19.962784365458525 9 26.417706956765883 17.12827044028506 20.52907754141194 35.92494506153711 10-11 25.195674113625767 26.310597307155074 26.967863571420587 21.52586500779857 12-13 26.56656082643385 23.746524234196002 27.376614749595824 22.310300189774328 14-15 23.09250967662378 25.147529145201254 26.281896485187644 25.478064692987317 16-17 22.11444787478435 28.571864595780838 26.73884103408955 22.574846495345263 18-19 22.03616989298866 27.24549118447802 28.907203901787415 21.8111350207459 20-21 23.11993660910884 25.951151620701946 28.868434632045997 22.06047713814322 22-23 22.496545120784635 26.109060129177276 28.942371497206565 22.45202325283153 24-25 22.690052192929706 26.237369087494738 28.978837223411354 22.093741496164203 26-27 22.33323635537926 26.288837009625652 29.220758485866643 22.157168149128438 28-29 21.896738386269906 27.00380697785128 28.958040236478173 22.141414399400645 30-31 22.168250667170835 26.269566634701114 28.989789763372727 22.57239293475533 32-33 21.990435062238785 26.484072124235286 29.00061955879539 22.524873254730544 34-35 22.81265398255423 26.385226614428923 28.646368964992458 22.155750438024384 36-37 22.647148860576475 26.039826171534823 28.92977229265642 22.38325267523228 38-39 22.313587836627402 26.470784386412692 28.91438494051874 22.301242836441162 40-41 22.517828831511622 26.52703460803707 28.70317276846738 22.251963791983933 42-43 22.289100000141886 26.560147788912708 28.24946544284885 22.90128676809655 44-45 22.43850660026497 26.430092880794408 28.28623672276404 22.845163796176582 46-47 22.870013771625132 26.78211302664495 27.90452264425844 22.443350557471483 48-49 22.808054917765592 26.849365262519786 27.710621144957237 22.631958674757385 50-51 22.306290788482595 27.047504898120682 28.058999852262712 22.587204461134007 52-53 22.83868683873366 27.21884696858555 27.32355707907032 22.61890911361047 54-55 22.353190724850094 26.839411246517418 27.68752102760684 23.11987700102564 56-57 22.785704112828174 27.046184826923735 27.377600480433866 22.79051057981422 58-59 23.21295012898517 26.75068217149131 27.328681176500847 22.707686523022677 60-61 22.740568527515435 26.84112005652924 28.098182664366146 22.320128751589174 62-63 22.47554218662429 27.00225955433217 27.96448562327518 22.557712635768354 64-65 22.35961349305246 27.33797791500352 27.606919554100294 22.69548903784373 66-67 22.47405252084409 27.12424728323185 27.259746499411445 23.141953696512616 68-69 22.341152143017652 27.0307352949262 27.6422912748852 22.985821287170946 70-71 22.711305637761157 26.956165171713042 27.40404824005525 22.92848095047055 72-73 22.603037645948564 26.765503099023285 27.785573133046903 22.845886121981245 74-75 22.2207164387622 27.60680532921994 27.361502560610397 22.81097567140746 76-77 22.447904331836966 27.049956670099352 27.36154167520403 23.140597322859648 78-79 22.56431333885132 27.139570881351112 27.358894879519035 22.937220900278536 80-81 22.710210849399974 27.27896926585131 27.225313280007263 22.78550660474145 82-83 22.56020386735001 27.186930666939098 27.58452378925383 22.668341676457068 84-85 22.440296539293016 27.323149187840677 27.275598896890006 22.9609553759763 86-87 22.513683105311138 27.310555943770687 27.24690245710617 22.928858493812008 88-89 22.444985069168492 27.259120736572317 26.899422222844944 23.396471971414247 90-91 22.505987418041588 26.92877513292219 27.32371503526116 23.241522413775062 92-93 22.801633815467824 26.95726346551502 27.406566266620263 22.834536452396893 94-95 22.362339084817766 26.86921470286953 27.462242857206203 23.306203355106494 96-97 22.01495522248807 26.844051443808475 27.784867863370632 23.356125470332824 98-99 22.178612482970028 27.107689742279746 27.083759082652136 23.629938692098094 100-101 22.006419389721597 26.797812284471135 27.39868546906176 23.7970828567455 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 181.0 1 157.5 2 189.0 3 253.5 4 231.0 5 232.5 6 414.0 7 868.0 8 1482.5 9 1919.5 10 1890.0 11 1754.5 12 1862.5 13 2072.0 14 2328.5 15 2554.0 16 2734.5 17 2938.5 18 3153.5 19 3540.0 20 4013.5 21 4578.0 22 5438.0 23 6572.5 24 8042.0 25 9977.5 26 12738.5 27 16237.0 28 19746.0 29 24046.0 30 29892.5 31 36346.0 32 43389.5 33 48868.0 34 53803.0 35 58667.0 36 64464.5 37 71014.5 38 74965.0 39 77632.5 40 80546.0 41 85605.0 42 90309.5 43 94289.5 44 99551.0 45 104576.5 46 106837.0 47 114527.5 48 133328.0 49 132428.5 50 113221.0 51 102303.5 52 96359.0 53 93420.0 54 90433.0 55 85028.5 56 78293.5 57 71160.5 58 65315.0 59 57935.5 60 48479.5 61 39710.0 62 32251.5 63 25146.5 64 18922.0 65 14775.0 66 11160.5 67 8597.0 68 6397.5 69 4312.5 70 3148.5 71 2333.5 72 1812.5 73 1533.5 74 1218.5 75 971.0 76 706.0 77 421.0 78 248.0 79 169.0 80 102.5 81 65.5 82 42.5 83 24.0 84 21.0 85 18.5 86 12.0 87 6.0 88 7.0 89 8.0 90 6.0 91 3.5 92 3.0 93 4.0 94 3.5 95 2.0 96 1.0 97 0.5 98 2.5 99 2.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07374395842245392 2 0.030930606899653594 3 0.015784541365075514 4 0.020927818888527086 5 0.0256454458583137 6 0.030292131069156156 7 0.0776457551643827 8 0.05607946044535818 9 0.0699131034394693 10-11 0.06067293933699251 12-13 0.04008209380345018 14-15 0.042972970480424685 16-17 0.05487345054330746 18-19 0.050670151325866 20-21 0.0181788257294409 22-23 0.013283844362293887 24-25 0.022453066705826516 26-27 0.024971499148344185 28-29 0.006384758304974366 30-31 0.0011882744623146735 32-33 0.009665814656141747 34-35 0.0108895599979285 36-37 0.004008209380345018 38-39 0.00918695778326867 40-41 0.004061415699553137 42-43 0.0020573110093806287 44-45 3.192379152487183E-4 46-47 4.0791511392891775E-4 48-49 6.030049510253567E-4 50-51 7.27153029177636E-4 52-53 7.094175894415962E-5 54-55 1.241480781522793E-4 56-57 0.0033874689895836215 58-59 0.0032455854716953022 60-61 0.005036864885035333 62-63 0.0026248450809339056 64-65 5.320631920811971E-4 66-67 3.3697335498475813E-4 68-69 0.003316527230639462 70-71 0.002234665406741028 72-73 0.020714993611694606 74-75 0.016050572961116113 76-77 0.02559223953910558 78-79 0.019615396348060133 80-81 0.011705390225786335 82-83 0.004469330813482056 84-85 0.003316527230639462 86-87 0.0015607186967715116 88-89 0.0016848667749237909 90-91 0.0062960811062941656 92-93 0.010322025926375223 94-95 0.013354786121238046 96-97 0.011794067424466537 98-99 0.023197955174740194 100-101 0.02007651778119717 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2819214.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.347867368096026 #Duplication Level Percentage of deduplicated Percentage of total 1 77.73180657215283 43.80021527010163 2 12.83913750997395 14.469160350655175 3 4.133851216375206 6.988011001792575 4 1.7719702867660023 3.9938698679559113 5 0.9392511566716971 2.6462399800733785 6 0.5631314566279273 1.9038753977323901 7 0.37303467789672323 1.471379598767749 8 0.25739051456053214 1.160272526101028 9 0.19497170423017257 0.988761575744407 >10 1.0605789552978067 11.093408085670877 >50 0.08709535989859861 3.3712369247415754 >100 0.043668521069152165 4.440703911369017 >500 0.0025305036797425256 0.9922376249962059 >1k 0.001328514431801415 1.5490456616733481 >5k 1.897877759716307E-4 0.7714393548331131 >10k+ 6.326259199054356E-5 0.3601428677916428 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10103 0.3583622953064223 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8351 0.2962173144713385 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6779 0.24045709194122902 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6511 0.23095089624271165 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4826 0.17118246433225714 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3881 0.13766248323114175 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3770 0.13372521560974088 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3222 0.11428717365904115 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2835 0.10055994330334624 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.547087947207981E-5 0.0 3.547087947207981E-5 0.0 6 0.0 3.547087947207981E-5 0.0 3.547087947207981E-5 0.0 7 0.0 3.547087947207981E-5 0.0 7.094175894415962E-5 0.0 8 0.0 3.547087947207981E-5 0.0 7.094175894415962E-5 0.0 9 0.0 3.547087947207981E-5 0.0 7.094175894415962E-5 0.0 10-11 0.0 3.547087947207981E-5 0.0 7.094175894415962E-5 0.0 12-13 0.0 1.0641263841623943E-4 0.0 7.094175894415962E-5 0.0 14-15 0.0 1.0641263841623943E-4 0.0 7.094175894415962E-5 0.0 16-17 0.0 1.0641263841623943E-4 0.0 2.1282527683247885E-4 0.0 18-19 0.0 1.0641263841623943E-4 0.0 2.6603159604059857E-4 0.0 20-21 0.0 1.0641263841623943E-4 0.0 2.8376703577663847E-4 0.0 22-23 0.0 1.0641263841623943E-4 0.0 3.369733549847582E-4 0.0 24-25 0.0 1.0641263841623943E-4 0.0 4.079151139289178E-4 0.0 26-27 0.0 1.0641263841623943E-4 0.0 6.384758304974366E-4 0.0 28-29 0.0 1.0641263841623943E-4 0.0 0.001099597263634474 0.0 30-31 0.0 1.0641263841623943E-4 0.0 0.002252400846477068 0.0 32-33 0.0 1.0641263841623943E-4 0.0 0.0037421777843044198 0.0 34-35 0.0 1.0641263841623943E-4 0.0 0.005143277523451572 0.0 36-37 0.0 1.2414807815227933E-4 0.0 0.007058705014943881 0.0 38-39 0.0 1.4188351788831923E-4 0.0 0.010747676480040182 0.0 40-41 0.0 1.4188351788831923E-4 0.0 0.015075123775633918 0.0 42-43 0.0 1.4188351788831923E-4 0.0 0.019367100191755575 0.0 44-45 0.0 1.4188351788831923E-4 0.0 0.0216904427971768 1.7735439736039904E-5 46-47 0.0 1.4188351788831923E-4 0.0 0.02562771041857766 3.547087947207981E-5 48-49 0.0 1.4188351788831923E-4 0.0 0.030150247551267835 3.547087947207981E-5 50-51 0.0 1.4188351788831923E-4 0.0 0.035932000905216845 3.547087947207981E-5 52-53 0.0 1.4188351788831923E-4 0.0 0.04776153920915546 3.547087947207981E-5 54-55 0.0 1.4188351788831923E-4 0.0 0.056842084354007893 3.547087947207981E-5 56-57 0.0 1.7735439736039904E-4 0.0 0.07755707796570249 3.547087947207981E-5 58-59 0.0 1.7735439736039904E-4 0.0 0.11026122883896008 3.547087947207981E-5 60-61 0.0 2.1282527683247885E-4 0.0 0.1376979541106138 3.547087947207981E-5 62-63 0.0 2.6603159604059857E-4 0.0 0.1532874056385929 3.547087947207981E-5 64-65 0.0 3.192379152487183E-4 0.0 0.18077733722945472 3.547087947207981E-5 66-67 0.0 3.192379152487183E-4 0.0 0.24114877409093455 3.547087947207981E-5 68-69 0.0 3.192379152487183E-4 0.0 0.32750263016571285 3.547087947207981E-5 70-71 0.0 3.547087947207981E-4 0.0 0.39851533086881663 3.547087947207981E-5 72-73 0.0 3.901796741928779E-4 0.0 0.4832729973673513 3.547087947207981E-5 74-75 0.0 3.901796741928779E-4 0.0 0.5814741271858043 3.547087947207981E-5 76-77 0.0 3.901796741928779E-4 0.0 0.6403203162299846 3.547087947207981E-5 78-79 0.0 3.901796741928779E-4 0.0 0.7264613470279304 3.547087947207981E-5 80-81 0.0 3.901796741928779E-4 0.0 0.8434975138460579 3.547087947207981E-5 82-83 0.0 3.901796741928779E-4 0.0 0.9522512303074545 3.547087947207981E-5 84-85 0.0 3.901796741928779E-4 0.0 1.1009806279338852 3.547087947207981E-5 86-87 0.0 3.901796741928779E-4 0.0 1.3233653067840896 3.547087947207981E-5 88-89 0.0 3.901796741928779E-4 0.0 1.6223848207337221 3.547087947207981E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 12875 0.0 24.327435 1 TACATGG 13355 0.0 23.131535 2 GAGTACT 9080 0.0 22.693216 12-13 ACATGGG 13145 0.0 22.386217 3 GTACTTT 9735 0.0 20.966948 14-15 GTACCTG 2590 0.0 20.736565 1 TACCTGG 2620 0.0 20.498032 2 AGTACTT 9575 0.0 20.204517 12-13 GTATCAA 25220 0.0 19.882248 1 AGAGTAC 17505 0.0 19.295692 10-11 CATGGGA 7820 0.0 18.480661 4 ACTTTTT 11845 0.0 17.892427 16-17 ATGGGAG 3645 0.0 17.737799 5 TATCAAC 28335 0.0 17.645239 2 ATCAACG 28135 0.0 17.623333 3 TCAACGC 28060 0.0 17.619612 4 CAACGCA 28070 0.0 17.528967 5 AACGCAG 28345 0.0 17.409527 6 TAAGGTG 1120 0.0 16.97854 5 GTGTAGC 1485 0.0 16.96319 1 >>END_MODULE