FastQCFastQC Report
Thu 26 May 2016
SRR936576_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936576_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2819214
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120920.428913874576389No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97410.3455218369375294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87170.30919965635811963No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT56870.20172289155771786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51340.18210749520965774No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46410.1646203516299224No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37310.13234185131032974No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT35810.1270212193895178No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC31220.11074008571183315No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30380.10776053183617845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG133400.024.0069391
TACATGG135900.023.3505572
ACATGGG133150.022.2598383
GAGTACT113050.021.42479112-13
TAAGGTG11750.021.4217535
GTAAGGT10750.020.7637774
GTACTTT117750.020.40792714-15
TACCTGG24300.019.5494252
AGTACTT118550.019.3892412-13
CATGGGG57000.018.9966494
GTACCTG25200.018.8552441
GTATAGG9000.018.478141
AGAGTAC201700.018.44705210-11
GTATCAA280500.017.8711411
ATAGCGC1350.00940555917.5919863
ACTTTTT138100.017.5726216-17
GTGTAGC16000.017.5212351
CATGGGA78050.017.2807434
TACTTTT133550.016.96226314-15
AGTACAT106450.016.0209792
Produced by FastQC (version 0.11.5)