FastQCFastQC Report
Thu 26 May 2016
SRR936575_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936575_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903124
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT61410.32267997250835995No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT50210.26382936687257375No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT36440.1914746490507187No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32590.17124475336341718No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC31620.16614787055388927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27460.144289074174883No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT22770.1196453830648975No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT19360.10172747545614474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG61200.025.4623741
TACATGG59500.025.1511942
ACATGGG60500.024.0287443
CATGGGG39300.022.727584
GAGTACT43800.021.53093112-13
GTATAGG5750.021.4823551
AGAGTAC72250.020.35350410-11
GTACCGT1200.00475647719.7975236
GTACTTT47850.019.6110814-15
GTGTAGC12650.019.5294151
AGTACTT48700.018.58413912-13
GTATAGC7650.018.0099721
GTATCAA123650.017.6357941
GAGTACA47200.017.1113151
ATGGGGG22650.016.9908875
TACTTTT53900.016.92500714-15
AGTACAT44550.016.742372
ACTTTTT58350.016.6129116-17
TCAACGC133750.016.3399944
GTACCTG16900.016.3048841
Produced by FastQC (version 0.11.5)