##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936575_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1903124 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.44000496026533 31.0 31.0 34.0 30.0 34.0 2 31.52793827412192 33.0 31.0 34.0 28.0 34.0 3 31.54663542680351 33.0 31.0 34.0 28.0 34.0 4 35.17390511600926 37.0 35.0 37.0 33.0 37.0 5 35.17421670894802 37.0 35.0 37.0 32.0 37.0 6 35.146555873395535 37.0 35.0 37.0 32.0 37.0 7 35.0651124151658 37.0 35.0 37.0 32.0 37.0 8 35.025427139797515 37.0 35.0 37.0 32.0 37.0 9 36.53896435544925 39.0 37.0 39.0 32.0 39.0 10-11 36.41865480126361 39.0 37.0 39.0 32.0 39.0 12-13 36.32149560406994 39.0 37.0 39.0 32.0 39.0 14-15 37.44831419287445 40.0 37.0 41.0 32.0 41.0 16-17 37.34066723975947 40.0 37.0 41.0 32.0 41.0 18-19 37.28312763645459 40.0 37.0 41.0 31.5 41.0 20-21 37.12174587677944 39.5 36.5 41.0 31.0 41.0 22-23 37.22818928246399 39.5 37.0 41.0 31.0 41.0 24-25 37.26669465573447 40.0 37.0 41.0 31.0 41.0 26-27 37.17420987807415 39.5 37.0 41.0 31.0 41.0 28-29 37.039419659465175 39.0 36.0 41.0 30.5 41.0 30-31 36.86498672708662 39.0 36.0 41.0 30.0 41.0 32-33 36.68293999760394 39.0 36.0 41.0 30.0 41.0 34-35 36.49774213346056 39.0 35.5 41.0 30.0 41.0 36-37 36.307354906984514 39.0 35.0 40.0 30.0 41.0 38-39 36.112017924213035 39.0 35.0 40.0 29.5 41.0 40-41 35.92012764276001 38.5 35.0 40.0 28.5 41.0 42-43 35.71219846946389 38.0 35.0 40.0 27.5 41.0 44-45 35.4826952946839 38.0 34.5 40.0 27.0 41.0 46-47 35.39843856732404 38.0 35.0 40.0 26.5 41.0 48-49 35.28779969145468 38.0 34.5 40.0 26.0 41.0 50-51 34.32467406222611 37.5 33.5 39.5 25.0 40.5 52-53 34.454271765791404 38.0 33.5 39.5 25.5 40.5 54-55 34.90575574686673 38.0 34.0 40.0 26.0 41.0 56-57 34.772594954401285 38.0 34.0 40.0 25.5 41.0 58-59 34.530181428009946 38.0 34.0 40.0 25.0 41.0 60-61 34.226441104205506 37.5 33.5 40.0 24.0 41.0 62-63 33.86028577223554 37.0 33.0 40.0 23.0 41.0 64-65 33.4925995363413 36.5 33.0 39.5 22.5 41.0 66-67 32.99406580968975 36.0 32.0 39.0 21.5 41.0 68-69 32.592958209764575 35.5 32.0 39.0 20.5 40.0 70-71 32.16883292943602 35.0 31.0 38.0 19.5 40.0 72-73 31.755011496886173 35.0 31.0 37.5 18.5 39.5 74-75 31.317775930522657 35.0 31.0 37.0 17.5 39.0 76-77 30.837277549965215 34.5 30.0 36.0 13.0 39.0 78-79 30.336974364255823 34.0 30.0 36.0 9.0 38.5 80-81 29.86855480777921 34.0 29.0 35.5 7.0 37.0 82-83 29.443181316614158 34.0 29.0 35.0 4.0 37.0 84-85 29.060608767479156 34.0 29.0 35.0 2.0 36.0 86-87 28.703104999989492 34.0 29.0 35.0 2.0 36.0 88-89 28.290080677874904 34.0 27.5 35.0 2.0 36.0 90-91 27.862640059186894 33.5 27.0 35.0 2.0 35.0 92-93 27.37979632435932 33.0 26.0 35.0 2.0 35.0 94-95 26.85171644096759 33.0 25.0 35.0 2.0 35.0 96-97 26.240395003163222 33.0 24.5 35.0 2.0 35.0 98-99 25.46986270994428 32.5 21.5 35.0 2.0 35.0 100-101 22.590077157347604 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3409810049398345 1101 2 0.3240769937292143 1101 3 0.36470355137605637 1101 4 0.44328049051291174 1101 5 0.3938605307155072 1101 6 0.46023682100070573 1101 7 0.5452174340324873 1101 8 0.6178715283546978 1101 9 0.4931233206838641 1101 10-11 0.6633774599650053 1101 12-13 0.7422541797638686 1101 14-15 0.7675791790939215 1101 16-17 0.8785768245492989 1101 18-19 0.7669032325828411 1101 20-21 0.6409252800935761 1101 22-23 0.6151668245605393 1101 24-25 0.7018458248569885 1101 26-27 0.6478002089798522 1101 28-29 0.5478738072468872 1101 30-31 0.560743010597939 1101 32-33 0.4584169084016665 1101 34-35 0.3683418565390326 1101 36-37 0.16669403815178896 1101 38-39 0.25378863119102846 1101 40-41 0.15224465036451562 1101 42-43 0.2930728411487351 1101 44-45 0.10692438161056117 1101 46-47 0.29969834609330803 1101 48-49 0.4172619433491178 1101 50-51 0.26150711816546846 1101 52-53 0.47620500188268267 1101 54-55 0.40844348417187604 1101 56-57 0.2772706209248952 1101 58-59 0.3641438142982949 1101 60-61 0.38649203287539535 1101 62-63 0.4168986146180558 1101 64-65 0.3177984075884055 1101 66-67 0.4690699258157167 1101 68-69 0.513014964228077 1101 70-71 0.4660107747477511 1101 72-73 0.4111047872062379 1101 74-75 0.36342698032251164 1101 76-77 0.3331445689572661 1101 78-79 0.26713784393102813 1101 80-81 0.4140184913546179 1101 82-83 0.1721264777939595 1101 84-85 0.23292797286735123 1101 86-87 0.175083318515874 1101 88-89 0.18615027541456897 1101 90-91 0.11364509314677562 1101 92-93 0.17096671511281514 1101 94-95 0.144974329299135 1101 96-97 0.007069410653063102 1101 98-99 -0.07911779292611598 1101 100-101 0.029521125864420128 1102 1 0.285336433482442 1102 2 0.31436751746322855 1102 3 0.33852118550940347 1102 4 0.214683188469742 1102 5 0.2666974837077589 1102 6 0.39279932829098385 1102 7 0.30070563874681966 1102 8 0.2799522779062471 1102 9 0.2310474575930357 1102 10-11 0.3498798732460813 1102 12-13 0.4192055326829731 1102 14-15 0.424329351696322 1102 16-17 0.21981019876076147 1102 18-19 0.22449919636628834 1102 20-21 0.2295623279777388 1102 22-23 0.11329676816132661 1102 24-25 0.2012285151215849 1102 26-27 -0.03781121335852333 1102 28-29 -0.179922942783854 1102 30-31 0.1323209906296512 1102 32-33 -0.030430927211490655 1102 34-35 0.03339625388080236 1102 36-37 -0.10739581913203011 1102 38-39 -0.14135639423432167 1102 40-41 -0.14829475184794916 1102 42-43 -0.16085867116356667 1102 44-45 -0.14898626551446625 1102 46-47 0.021062307026845417 1102 48-49 0.13783264255435768 1102 50-51 0.2126302948062957 1102 52-53 0.42322887145517285 1102 54-55 0.11438396235146087 1102 56-57 0.0677651938009447 1102 58-59 0.0759996684358697 1102 60-61 0.04153864340555913 1102 62-63 0.1782712608414485 1102 64-65 0.12119757153073607 1102 66-67 0.11465887043478062 1102 68-69 0.07288593013949196 1102 70-71 0.054004110245834624 1102 72-73 -0.17714134382026714 1102 74-75 -0.13923673187440855 1102 76-77 -0.06189957469837282 1102 78-79 -0.02594872050878294 1102 80-81 0.053161248516438775 1102 82-83 0.05759493174931052 1102 84-85 -0.3114568532534605 1102 86-87 -0.18290982782889742 1102 88-89 -0.21658886166799363 1102 90-91 -0.31766880520853746 1102 92-93 -0.234272201290878 1102 94-95 -0.3018588106774729 1102 96-97 -0.42646565994841623 1102 98-99 -0.45800075588674716 1102 100-101 -0.21171004155833373 1103 1 0.07078711177973318 1103 2 -0.10680386925427499 1103 3 0.05371958332074911 1103 4 0.2910366142389975 1103 5 0.3189802239828836 1103 6 0.2374633144349545 1103 7 0.30604445987828655 1103 8 0.23853326822795395 1103 9 -0.10148899232644482 1103 10-11 0.21286143795335732 1103 12-13 -0.02472637132181177 1103 14-15 0.24118706196338024 1103 16-17 0.3488223695758137 1103 18-19 0.3945851564162055 1103 20-21 0.40984029290721224 1103 22-23 -0.07333765524556668 1103 24-25 0.2104031484956721 1103 26-27 -0.022208929427279145 1103 28-29 0.18818312246233404 1103 30-31 0.0564022672083766 1103 32-33 -0.04650491848511251 1103 34-35 0.22776434894024788 1103 36-37 0.34653689728126125 1103 38-39 0.33242859781598355 1103 40-41 0.10005639917751807 1103 42-43 0.013837388102274417 1103 44-45 0.2809539225049349 1103 46-47 0.38471533161092 1103 48-49 0.37644206930020374 1103 50-51 0.4084219124519777 1103 52-53 0.5020532844581282 1103 54-55 0.5006122665784503 1103 56-57 0.5063069162941147 1103 58-59 0.41928963149041465 1103 60-61 0.3936363799759377 1103 62-63 0.4416045963015023 1103 64-65 0.42456132552153036 1103 66-67 0.4109268104135282 1103 68-69 0.37615738431668433 1103 70-71 0.43455528475033134 1103 72-73 0.15691055632239426 1103 74-75 0.1129948925938038 1103 76-77 0.2923197283827399 1103 78-79 0.23766151439211924 1103 80-81 0.2379054929637725 1103 82-83 -0.10893169015406912 1103 84-85 0.04524545305091365 1103 86-87 0.26682994624497525 1103 88-89 0.12226337313592595 1103 90-91 0.11258225666466615 1103 92-93 -0.024476000980712342 1103 94-95 -0.11014864187514917 1103 96-97 -0.12039150279032995 1103 98-99 -0.19180080939511512 1103 100-101 0.06235979324626584 1104 1 0.36964664468182207 1104 2 0.34509815231144003 1104 3 0.2449198266829704 1104 4 0.413514685241438 1104 5 0.34465477059085714 1104 6 0.29366372959213294 1104 7 0.42746578239775346 1104 8 0.36837086752334613 1104 9 0.38567289634539037 1104 10-11 0.5592809377702537 1104 12-13 0.5171400593572457 1104 14-15 0.2809513090028517 1104 16-17 0.19963227803843608 1104 18-19 0.32551352797601396 1104 20-21 0.06642552876492402 1104 22-23 0.2058308236483768 1104 24-25 0.20968496177884788 1104 26-27 0.018445299284593375 1104 28-29 0.13953581918222824 1104 30-31 0.24045542140566312 1104 32-33 0.138445759602142 1104 34-35 0.18586733037884784 1104 36-37 0.048809954049851 1104 38-39 0.0038732593086905354 1104 40-41 -0.062438583152740534 1104 42-43 0.14113326007899474 1104 44-45 0.0932398597050792 1104 46-47 -0.11678556968407605 1104 48-49 0.1261808152640711 1104 50-51 -0.001560882911739725 1104 52-53 -0.01820427389140633 1104 54-55 -0.13165560282050848 1104 56-57 -0.18286698461623274 1104 58-59 -0.15171364267003185 1104 60-61 -0.10955523508938825 1104 62-63 0.05957535428598959 1104 64-65 0.08784279353876201 1104 66-67 0.18479897653003263 1104 68-69 0.013304230454608046 1104 70-71 -0.14950566307177127 1104 72-73 -0.18179032184313826 1104 74-75 -0.13612958165078126 1104 76-77 -0.07557707396841806 1104 78-79 0.145663220609336 1104 80-81 0.22683351758157144 1104 82-83 0.1540553799709521 1104 84-85 -0.07883785325613957 1104 86-87 -0.12436434714560818 1104 88-89 -0.10731502528255632 1104 90-91 -0.26959405859332364 1104 92-93 -0.4943921901831061 1104 94-95 -0.37502336145130855 1104 96-97 -0.4240767978963511 1104 98-99 -0.3084621546082822 1104 100-101 -0.26410383692360995 1105 1 0.371848756295698 1105 2 0.27692221692487706 1105 3 0.339129917667254 1105 4 0.45080656477240666 1105 5 0.3659798465269404 1105 6 0.49754595742123087 1105 7 0.5125140548084559 1105 8 0.573762225058914 1105 9 0.5064566540171995 1105 10-11 0.5492452113208657 1105 12-13 0.4361048264055043 1105 14-15 0.42388706394999787 1105 16-17 0.4515013134938073 1105 18-19 0.3641935955365412 1105 20-21 0.4537934486375903 1105 22-23 0.17595531016754506 1105 24-25 0.26310990495711906 1105 26-27 0.02818819396107841 1105 28-29 0.22582124153974803 1105 30-31 0.14023821293418592 1105 32-33 -0.11764483544480697 1105 34-35 0.11025257827453316 1105 36-37 0.14765357090106335 1105 38-39 0.11644611971836127 1105 40-41 0.09947034915467157 1105 42-43 0.17445581987214354 1105 44-45 0.2436723999668402 1105 46-47 0.5270033106323169 1105 48-49 0.45994863504706984 1105 50-51 0.5184407852493251 1105 52-53 0.5233243176941187 1105 54-55 0.45460535317479867 1105 56-57 0.25771284036586195 1105 58-59 0.48319888313434234 1105 60-61 0.5824432218616238 1105 62-63 0.3893537668917233 1105 64-65 0.27987391864389366 1105 66-67 0.5281729712892229 1105 68-69 0.6749173422005441 1105 70-71 0.5161004702003922 1105 72-73 0.43811980597969935 1105 74-75 0.5627970262132074 1105 76-77 0.3209585030415383 1105 78-79 0.4325195710899763 1105 80-81 0.6622663011168228 1105 82-83 0.5178732445857825 1105 84-85 0.33741483144056517 1105 86-87 0.25792436983001465 1105 88-89 0.2597526951058491 1105 90-91 0.35011568138206783 1105 92-93 0.03427918903855698 1105 94-95 0.29286127130998096 1105 96-97 0.21655626922627746 1105 98-99 0.3774967252215653 1105 100-101 0.1356579635782289 1106 1 0.23346798463219187 1106 2 0.12594182476801308 1106 3 0.258914863903815 1106 4 0.2576503345383756 1106 5 0.22423411597665677 1106 6 0.3525364697551936 1106 7 0.4418625684074442 1106 8 0.46278183290205277 1106 9 0.5355015623030894 1106 10-11 0.60061143522978 1106 12-13 0.44130406739221684 1106 14-15 0.5228560709076149 1106 16-17 0.516846032026379 1106 18-19 0.47872869990086286 1106 20-21 0.20428680727136594 1106 22-23 0.43326081301385244 1106 24-25 0.3591756861082871 1106 26-27 0.3956897752387505 1106 28-29 0.37140631427852355 1106 30-31 0.47050386758314033 1106 32-33 0.3729370747386227 1106 34-35 0.2739401178064753 1106 36-37 0.14915262213445857 1106 38-39 0.2188939375551726 1106 40-41 -0.015861719156525567 1106 42-43 0.11933248021369991 1106 44-45 0.026771704204662683 1106 46-47 0.15414436060068937 1106 48-49 0.1731617912773089 1106 50-51 -0.05341247218267853 1106 52-53 -0.014841400205945376 1106 54-55 0.19912780731774404 1106 56-57 0.055549652510080705 1106 58-59 0.10154170413370878 1106 60-61 0.252025764556123 1106 62-63 0.32307293197711573 1106 64-65 0.3278498832232728 1106 66-67 0.2997605740722804 1106 68-69 0.33260235168820884 1106 70-71 0.08218522668363448 1106 72-73 -0.07820530470973353 1106 74-75 0.28873630515058935 1106 76-77 0.22072447394603145 1106 78-79 0.14589718019813702 1106 80-81 0.423309944380577 1106 82-83 0.01882201118919724 1106 84-85 0.11591578020717108 1106 86-87 0.2023519473126214 1106 88-89 0.27327578175986744 1106 90-91 0.20548570035740354 1106 92-93 0.2052722314167994 1106 94-95 0.3240124414554586 1106 96-97 0.36200851464057493 1106 98-99 0.4150236069419968 1106 100-101 0.2598262115225687 1107 1 0.2143091958367087 1107 2 0.1872222575903777 1107 3 0.1333288957255654 1107 4 0.22271059419301054 1107 5 0.17134698526701442 1107 6 0.383197047433427 1107 7 0.4970448987059797 1107 8 0.3048317277021724 1107 9 0.43448543811875595 1107 10-11 0.5051125357077879 1107 12-13 0.36852902060978465 1107 14-15 -0.08205787849948365 1107 16-17 0.23566740553928156 1107 18-19 0.17681386817218936 1107 20-21 0.11315287861336998 1107 22-23 0.24574314234279626 1107 24-25 0.25539760452747373 1107 26-27 0.23326377874865045 1107 28-29 0.3165648897655018 1107 30-31 0.22292443440203868 1107 32-33 0.11531614335608964 1107 34-35 0.19156289139888116 1107 36-37 0.24617599880820507 1107 38-39 0.14125419624021873 1107 40-41 0.14972318247916405 1107 42-43 0.16932666270882635 1107 44-45 0.21792017662484398 1107 46-47 0.24667303605178859 1107 48-49 0.19250596456138425 1107 50-51 0.10583377310271658 1107 52-53 0.27560441458408746 1107 54-55 0.4782921227428645 1107 56-57 0.32509133008456814 1107 58-59 0.44789946129166225 1107 60-61 0.3513166107122103 1107 62-63 0.4011388055239493 1107 64-65 0.2965098209759631 1107 66-67 0.29634443825293033 1107 68-69 0.3580754602719338 1107 70-71 0.24679443579385207 1107 72-73 0.20083024994087495 1107 74-75 0.24136135726573826 1107 76-77 0.24183086641430052 1107 78-79 0.3222013193528497 1107 80-81 0.2591766071689108 1107 82-83 0.3446097498280949 1107 84-85 0.3258957098153594 1107 86-87 0.3604184026099446 1107 88-89 0.06411893213267206 1107 90-91 -0.03493868662302191 1107 92-93 0.3347190836618701 1107 94-95 0.39481816588025254 1107 96-97 0.42214105679324376 1107 98-99 0.47636056652003234 1107 100-101 0.5275625234183963 1108 1 0.27713733947090446 1108 2 0.2373127925099503 1108 3 0.22921728325865232 1108 4 0.330116732387836 1108 5 0.1435015890949316 1108 6 0.26870582459390135 1108 7 0.3055453641063721 1108 8 0.16674688666549287 1108 9 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60-61 -2.350316039856601 2316 62-63 -2.166466619953759 2316 64-65 -2.216936155272812 2316 66-67 -2.0987238551614418 2316 68-69 -2.311273955989165 2316 70-71 -2.1699907970062604 2316 72-73 -1.858253849577327 2316 74-75 -1.8503136717906017 2316 76-77 -1.9522238985872846 2316 78-79 -2.9869346846529545 2316 80-81 -2.919548862872027 2316 82-83 -2.6387779305569588 2316 84-85 -2.3914290582597815 2316 86-87 -2.2915192631536527 2316 88-89 -2.6501840109535486 2316 90-91 -2.5395882092035116 2316 92-93 -3.136386061260211 2316 94-95 -3.19370934790836 2316 96-97 -3.5700218744465424 2316 98-99 -5.123564222859699 2316 100-101 -6.229711398613599 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4692.0 3 1803.0 4 1852.0 5 1992.0 6 2203.0 7 2600.0 8 3417.0 9 4169.0 10 5266.0 11 6197.0 12 7256.0 13 8539.0 14 9695.0 15 10875.0 16 12471.0 17 15166.0 18 13354.0 19 11851.0 20 12511.0 21 13663.0 22 15036.0 23 17457.0 24 19601.0 25 22841.0 26 26548.0 27 31155.0 28 36769.0 29 44197.0 30 53519.0 31 65910.0 32 81960.0 33 104665.0 34 138095.0 35 189677.0 36 267203.0 37 346715.0 38 258392.0 39 33805.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0872567515726 17.881432978259383 14.278912601358988 23.75239766880902 2 15.55865487291164 19.049853699141533 40.28917541809254 25.102316009854285 3 20.16691681591538 22.615121206770425 29.058881769795423 28.159080207518773 4 12.963688030183507 15.57053145402917 36.91088896608665 34.55489154970067 5 14.319383932565193 36.33314568737553 34.34199605890927 15.005474321150011 6 33.292170426001924 34.635248275941834 17.225967012897712 14.846614285158537 7 29.516613916983225 31.34171968347037 20.190508420068596 18.951157979477806 8 26.294049506157783 34.180966156356035 19.55386012639333 19.971124211092846 9 25.66091133829651 17.086476055910104 20.32142370917746 36.93118889661592 10-11 25.576753547887876 25.559090987941623 27.076205009753835 21.787950454416666 12-13 26.283717087108588 22.82943377264494 27.42748835493098 23.459360785315493 14-15 23.005601796275 24.627116310067677 25.820507052439705 26.546774841217612 16-17 22.679061788054252 27.61321292468397 26.49441428504977 23.213311002212002 18-19 22.479823239171612 26.957734442503327 28.106979403831637 22.45546291449342 20-21 23.34523120145161 25.75170404826299 27.952673839734427 22.950390910550972 22-23 22.87923985835367 25.662531855840676 28.09867106064217 23.35955722516348 24-25 23.113182265305777 25.682922138521295 28.144021876208576 23.05987371996435 26-27 22.794940798110876 25.781084768508748 28.40229908310439 23.021675350275984 28-29 22.50516059520047 26.571239333653335 28.031391551835704 22.892208519310493 30-31 22.774612155848086 25.88474812462298 27.87488623728643 23.46575348224251 32-33 22.67726800529454 25.889827769846246 28.102462675234474 23.33044154962474 34-35 23.31088865226035 25.926378398459164 27.76154569054504 23.001187258735442 36-37 23.07331361657196 25.697585305381665 28.218973227363403 23.01012785068297 38-39 22.827656744294313 26.123055072358675 28.02513867334411 23.024149510002896 40-41 22.94417435936239 26.086199177764325 27.93996019135055 23.029666271522732 42-43 22.720564647178275 26.024945554919448 27.523738685683014 23.730751112219263 44-45 22.901728840339942 25.871152618902933 27.60078114798698 23.626337392770154 46-47 23.303934086836808 26.24220355102963 27.46013104865194 22.993731313481618 48-49 23.193940225116087 26.10696613485211 27.42964531158784 23.26944832844396 50-51 22.683414040879853 26.353941235307577 27.794013287516005 23.168631436296565 52-53 23.126432058457016 26.5677337212672 27.00077007009976 23.30506415017602 54-55 22.80604680528067 26.19743680488394 27.29349054375479 23.703025846080603 56-57 23.11498827052435 26.221558747346123 27.07424131481524 23.58921166731429 58-59 23.438000434569332 26.164121651037668 27.08546644081673 23.312411473576265 60-61 23.06662399905837 26.16882710843405 27.694903480300127 23.069645412207453 62-63 22.796676719459814 26.343379908618452 27.58781043439469 23.272132937527044 64-65 22.643125950416003 26.607628339658056 27.298443498392633 23.450802211533308 66-67 22.8596698717435 26.382539184961217 26.891166563707962 23.86662437958732 68-69 22.734971209778056 26.264362257422537 27.32098495465159 23.679681578147818 70-71 23.191548644187197 26.0620183158271 27.095306437876545 23.65112660210916 72-73 22.92738197027927 26.06328832533567 27.468603623938236 23.540726080446824 74-75 22.66040806200507 26.78113209927287 27.028221012386506 23.530238826335548 76-77 22.93245945754178 26.252828314416586 26.981396852260154 23.83331537578148 78-79 22.850254156043427 26.27563674389004 27.178332977144226 23.695776122922307 80-81 23.15059582373861 26.269540236836058 26.936039329993434 23.6438246094319 82-83 23.054857684181528 26.21780526155588 27.34202216050463 23.385314893757954 84-85 22.93068110153458 26.445527643472776 27.040230187092707 23.583561067899936 86-87 22.88225180504348 26.51669768684831 27.083443368920484 23.517607139187728 88-89 22.81428590949272 26.324089130318246 26.768631795587634 24.0929931646014 90-91 22.755521376938052 26.11260686366446 27.06210602893992 24.06976573045756 92-93 23.03821864271734 26.241430526561427 27.255839645469244 23.46451118525199 94-95 22.750958085956437 26.165488410345883 27.258383005782015 23.825170497915664 96-97 22.33237201174608 26.23151864723001 27.633427713653173 23.80268162737073 98-99 22.473794736630435 26.41202977793771 26.951919844239125 24.162255641192726 100-101 22.530639180028107 26.076489169971694 27.025603745979083 24.367267904021116 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 51.0 1 41.5 2 48.5 3 72.0 4 75.5 5 81.5 6 131.0 7 264.5 8 450.0 9 605.0 10 622.5 11 588.5 12 634.5 13 701.0 14 818.0 15 964.0 16 1054.0 17 1187.5 18 1338.0 19 1530.0 20 1722.0 21 1959.0 22 2426.5 23 3028.5 24 3846.5 25 4966.0 26 6395.0 27 8418.0 28 10829.0 29 13648.5 30 17129.5 31 21136.5 32 25258.5 33 28550.5 34 31652.0 35 34973.0 36 39244.0 37 43479.5 38 46241.5 39 49155.5 40 52486.0 41 55860.5 42 58831.5 43 61390.0 44 64475.5 45 68380.5 46 71711.0 47 78353.0 48 89798.5 49 89671.5 50 78694.0 51 73281.0 52 72270.5 53 71132.5 54 69672.0 55 65534.5 56 59754.0 57 54264.5 58 49854.5 59 44351.0 60 36766.0 61 30699.0 62 25104.5 63 19400.0 64 14071.0 65 9997.5 66 7450.5 67 5950.5 68 4797.0 69 3790.0 70 2954.0 71 2096.5 72 1478.5 73 1070.5 74 784.0 75 554.0 76 386.5 77 242.0 78 143.5 79 100.0 80 64.5 81 43.0 82 32.0 83 16.0 84 14.5 85 13.5 86 10.0 87 8.5 88 5.5 89 3.0 90 5.0 91 4.5 92 2.0 93 2.0 94 2.5 95 1.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012295572963191047 2 0.010298855986262588 3 0.013661747736878942 4 0.019073901648027136 5 0.03178983608004523 6 0.04865684001673039 7 0.00583251537997524 8 0.02585223033286323 9 0.03746471590921033 10-11 0.04177342096468754 12-13 0.031632200529235086 14-15 0.027323495473757883 16-17 0.028768488022850848 18-19 0.02311988078548744 20-21 0.05057473921825377 22-23 0.011323487066528507 24-25 0.002732349547375788 26-27 9.195407130591595E-4 28-29 2.6272591801690273E-5 30-31 0.002101807344135222 32-33 0.0016289006917047967 34-35 2.6272591801690273E-5 36-37 2.88998509818593E-4 38-39 2.6272591801690273E-5 40-41 0.001050903672067611 42-43 0.0031264384244011423 44-45 0.001444992549092965 46-47 7.356325704473277E-4 48-49 8.932681212574694E-4 50-51 0.0031264384244011423 52-53 0.0026535317719707176 54-55 0.0036518902604349484 56-57 6.568147950422568E-4 58-59 0.00475533911610594 60-61 0.0021543525277386024 62-63 2.3645332621521247E-4 64-65 5.254518360338055E-4 66-67 0.01353038477787049 68-69 0.03898852623370837 70-71 0.049602653321591236 72-73 0.014555015858136412 74-75 0.02995075465392691 76-77 0.02569459478205309 78-79 0.05488344427373098 80-81 0.07729396508057278 82-83 0.047868662262679676 84-85 0.0170246394874953 86-87 0.01736618318091727 88-89 0.02007226013649137 90-91 0.03678162852236638 92-93 0.02311988078548744 94-95 0.012689661840216401 96-97 0.018995083872622068 98-99 0.05136291697230448 100-101 0.07101481563996881 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1903124.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.93323647276877 #Duplication Level Percentage of deduplicated Percentage of total 1 81.43260612336121 53.69115276125412 2 11.376844262270074 15.002243261162304 3 3.33786030199896 6.6022779781429435 4 1.3446831626651459 3.5463725177980665 5 0.7133179324929251 2.351567996166127 6 0.41414675926916467 1.6383621727994804 7 0.28384517986918256 1.310038195617928 8 0.18679474889300923 0.9852785880507392 9 0.13963188450631145 0.8285743854263674 >10 0.7160959821673881 8.227491255455403 >50 0.03792538886268942 1.690457204482177 >100 0.014178098328074712 1.705526465684043 >500 7.962212753022646E-4 0.3814464399414308 >1k 0.001114709785423168 1.4532342899062305 >5k 1.5924425506045259E-4 0.5859764881126128 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6141 0.32267997250835995 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5021 0.26382936687257375 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3644 0.1914746490507187 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3259 0.17124475336341718 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3162 0.16614787055388927 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2746 0.144289074174883 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2277 0.1196453830648975 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1936 0.10172747545614474 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.2545183603380545E-5 2 0.0 0.0 0.0 0.0 5.2545183603380545E-5 3 0.0 0.0 0.0 0.0 5.2545183603380545E-5 4 0.0 0.0 0.0 0.0 1.0509036720676109E-4 5 0.0 0.0 0.0 0.0 1.0509036720676109E-4 6 2.6272591801690273E-4 0.0 0.0 0.0 1.5763555081014163E-4 7 2.6272591801690273E-4 0.0 0.0 0.0 1.5763555081014163E-4 8 3.6781628522366384E-4 0.0 0.0 0.0 1.5763555081014163E-4 9 3.6781628522366384E-4 0.0 0.0 0.0 1.5763555081014163E-4 10-11 3.6781628522366384E-4 0.0 0.0 0.0 1.5763555081014163E-4 12-13 3.6781628522366384E-4 0.0 0.0 0.0 2.3645332621521247E-4 14-15 3.6781628522366384E-4 0.0 0.0 0.0 2.6272591801690273E-4 16-17 4.7290665243042494E-4 0.0 0.0 0.0 2.88998509818593E-4 18-19 4.7290665243042494E-4 0.0 0.0 7.881777540507081E-5 3.6781628522366384E-4 20-21 4.7290665243042494E-4 0.0 0.0 1.0509036720676109E-4 4.7290665243042494E-4 22-23 4.7290665243042494E-4 0.0 0.0 1.5763555081014163E-4 5.254518360338055E-4 24-25 4.7290665243042494E-4 0.0 0.0 1.839081426118319E-4 5.254518360338055E-4 26-27 4.7290665243042494E-4 0.0 0.0 3.1527110162028326E-4 5.254518360338055E-4 28-29 4.7290665243042494E-4 0.0 0.0 0.0010771762638693012 5.254518360338055E-4 30-31 4.991792442321152E-4 5.2545183603380545E-5 0.0 0.002285715486747054 5.254518360338055E-4 32-33 5.254518360338055E-4 5.2545183603380545E-5 0.0 0.004098524321063683 5.77997019637186E-4 34-35 5.254518360338055E-4 5.2545183603380545E-5 0.0 0.005569789461958338 5.77997019637186E-4 36-37 5.254518360338055E-4 5.2545183603380545E-5 0.0 0.007724141989696941 5.77997019637186E-4 38-39 5.254518360338055E-4 5.2545183603380545E-5 0.0 0.01155994039274372 5.77997019637186E-4 40-41 5.254518360338055E-4 5.2545183603380545E-5 0.0 0.01610509877443614 5.77997019637186E-4 42-43 5.254518360338055E-4 5.2545183603380545E-5 0.0 0.02235797562323842 5.77997019637186E-4 44-45 5.254518360338055E-4 5.2545183603380545E-5 0.0 0.02777012953438662 6.042696114388763E-4 46-47 5.254518360338055E-4 5.2545183603380545E-5 0.0 0.04043351878280133 6.305422032405665E-4 48-49 5.254518360338055E-4 5.2545183603380545E-5 0.0 0.05469953613111915 6.830873868439471E-4 50-51 5.77997019637186E-4 5.2545183603380545E-5 0.0 0.0618456811011789 7.356325704473277E-4 52-53 5.77997019637186E-4 5.2545183603380545E-5 0.0 0.07316916816770741 8.407229376540887E-4 54-55 5.77997019637186E-4 5.2545183603380545E-5 0.0 0.09607886821878134 8.407229376540887E-4 56-57 5.77997019637186E-4 5.2545183603380545E-5 0.0 0.12897215315449756 8.66995529455779E-4 58-59 5.77997019637186E-4 5.2545183603380545E-5 0.0 0.1658063268604673 8.932681212574692E-4 60-61 5.77997019637186E-4 5.2545183603380545E-5 0.0 0.2085255611300157 9.195407130591596E-4 62-63 5.77997019637186E-4 5.2545183603380545E-5 0.0 0.2531626946010875 9.458133048608499E-4 64-65 5.77997019637186E-4 5.2545183603380545E-5 0.0 0.30063726798674184 9.983584884642304E-4 66-67 6.042696114388763E-4 5.2545183603380545E-5 0.0 0.36960282146617873 0.0010246310802659207 68-69 6.305422032405665E-4 5.2545183603380545E-5 0.0 0.4553565611068958 0.001050903672067611 70-71 6.305422032405665E-4 5.2545183603380545E-5 0.0 0.5016225952696725 0.0012085392228777525 72-73 7.356325704473277E-4 5.2545183603380545E-5 0.0 0.5663319888772356 0.0012085392228777525 74-75 7.356325704473277E-4 5.2545183603380545E-5 0.0 0.6397901555547616 0.0012085392228777525 76-77 7.356325704473277E-4 5.2545183603380545E-5 0.0 0.7170053028599292 0.0012085392228777525 78-79 7.356325704473277E-4 5.2545183603380545E-5 0.0 0.808460194921613 0.0012085392228777525 80-81 7.356325704473277E-4 5.2545183603380545E-5 0.0 0.9431072279052758 0.0012085392228777525 82-83 7.356325704473277E-4 5.2545183603380545E-5 0.0 1.0945161744584169 0.001261084406481133 84-85 7.356325704473277E-4 5.2545183603380545E-5 0.0 1.3064571725226521 0.001261084406481133 86-87 7.356325704473277E-4 5.2545183603380545E-5 0.0 1.6070418953257906 0.0012873569982828235 88-89 7.881777540507082E-4 5.2545183603380545E-5 0.0 1.9351602943371002 0.0013136295900845138 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6120 0.0 25.462374 1 TACATGG 5950 0.0 25.151194 2 ACATGGG 6050 0.0 24.028744 3 CATGGGG 3930 0.0 22.72758 4 GAGTACT 4380 0.0 21.530931 12-13 GTATAGG 575 0.0 21.482355 1 AGAGTAC 7225 0.0 20.353504 10-11 GTACCGT 120 0.004756477 19.797523 6 GTACTTT 4785 0.0 19.61108 14-15 GTGTAGC 1265 0.0 19.529415 1 AGTACTT 4870 0.0 18.584139 12-13 GTATAGC 765 0.0 18.009972 1 GTATCAA 12365 0.0 17.635794 1 GAGTACA 4720 0.0 17.111315 1 ATGGGGG 2265 0.0 16.990887 5 TACTTTT 5390 0.0 16.925007 14-15 AGTACAT 4455 0.0 16.74237 2 ACTTTTT 5835 0.0 16.61291 16-17 TCAACGC 13375 0.0 16.339994 4 GTACCTG 1690 0.0 16.304884 1 >>END_MODULE