FastQCFastQC Report
Thu 26 May 2016
SRR936575_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936575_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1903124
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT94160.49476544880943124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36810.1934188208440438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34190.17965198273995808No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30830.1619968010492222No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT29400.15448283979393881No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT26330.138351468427701No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23480.12337609110073752No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC23220.12200991632704963No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22320.11728084980274538No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19400.10193765619055827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG59950.024.095291
TACATGG58500.023.794042
TAAGGTG8750.023.3413285
ACATGGG58350.022.7135773
GAGTACT52350.020.05226912-13
GTACGAT1456.1453413E-419.6564673
GTAAGGT7200.019.1311934
GTATACG1250.006020210719.0067441
GTACTTT55800.018.64223114-15
AGAGTAC80050.018.3940310-11
CATGGGG39500.018.0372664
AGTACTT55850.017.94515612-13
GTACCGT2152.3981966E-517.672336
GTGTAGG7700.016.6617571
GAGTACA48700.016.5869961
GTACCTG16150.016.4764351
AGTACAT44900.016.1883132
ACTTTTT65850.016.01345816-17
TACTTTT61900.015.99938814-15
GGACCAT9800.015.993016
Produced by FastQC (version 0.11.5)