##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936575_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1903124 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.988066463351835 31.0 31.0 34.0 30.0 34.0 2 32.127124664499 33.0 31.0 34.0 30.0 34.0 3 32.244182197271435 34.0 31.0 34.0 30.0 34.0 4 35.94490427318451 37.0 35.0 37.0 35.0 37.0 5 35.804162524354695 37.0 35.0 37.0 35.0 37.0 6 35.84600215225072 37.0 35.0 37.0 35.0 37.0 7 35.778029177289554 37.0 35.0 37.0 33.0 37.0 8 35.74542856902651 37.0 35.0 37.0 33.0 37.0 9 37.3799783934205 39.0 37.0 39.0 34.0 39.0 10-11 37.277491640061285 39.0 37.0 39.0 34.0 39.0 12-13 37.16225295881929 39.0 37.0 39.0 33.0 39.0 14-15 38.297709187630446 40.0 38.0 41.0 33.0 41.0 16-17 38.258439544664455 40.0 38.0 41.0 33.0 41.0 18-19 38.37360650173083 40.0 38.0 41.0 33.5 41.0 20-21 38.371649456367535 40.0 38.0 41.0 34.0 41.0 22-23 38.26502214254037 40.0 38.0 41.0 34.0 41.0 24-25 38.15136848676177 40.0 37.5 41.0 33.0 41.0 26-27 37.950479317164834 40.0 37.0 41.0 33.0 41.0 28-29 38.014106805442 40.0 37.0 41.0 33.0 41.0 30-31 37.94009849069215 40.0 37.0 41.0 33.0 41.0 32-33 37.782256700036356 40.0 37.0 41.0 33.0 41.0 34-35 37.61576124309293 40.0 37.0 41.0 32.5 41.0 36-37 37.46668609086954 40.0 37.0 41.0 32.0 41.0 38-39 37.27920461304676 39.0 36.0 41.0 31.5 41.0 40-41 37.09158231413192 39.0 36.0 41.0 31.0 41.0 42-43 36.86247611821405 39.0 36.0 41.0 31.0 41.0 44-45 36.62615152769867 39.0 35.0 40.0 30.0 41.0 46-47 36.33310940327587 39.0 35.0 40.0 30.0 41.0 48-49 35.990067383943455 39.0 35.0 40.0 29.0 41.0 50-51 35.70836293378676 38.0 35.0 40.0 28.0 41.0 52-53 35.46899518896299 38.0 34.5 40.0 28.0 41.0 54-55 35.14804263936559 38.0 34.0 40.0 27.0 41.0 56-57 34.79157637652618 38.0 34.0 40.0 26.5 41.0 58-59 34.40944941054813 37.0 33.0 40.0 26.0 41.0 60-61 34.0048149778995 37.0 33.0 39.5 25.5 40.5 62-63 33.67167220843203 36.0 32.0 39.0 25.0 40.0 64-65 33.34552583016136 36.0 32.0 39.0 24.5 40.0 66-67 32.88725642680141 35.5 31.5 38.5 23.0 40.0 68-69 32.39951889629893 35.0 31.0 38.0 22.0 40.0 70-71 31.87013300236874 35.0 31.0 37.5 21.0 39.5 72-73 31.28899378075207 34.0 30.0 37.0 19.5 39.0 74-75 30.752493531687897 34.0 29.5 36.0 18.0 39.0 76-77 29.37945110250304 32.5 28.0 34.5 17.5 36.5 78-79 30.175484624228375 34.0 29.5 35.0 18.0 37.0 80-81 30.01355586919192 34.0 29.0 35.0 16.0 37.0 82-83 29.608383373863184 34.0 29.0 35.0 11.0 36.5 84-85 29.15321203452849 33.5 29.0 35.0 7.0 36.0 86-87 28.744461474922286 33.0 29.0 35.0 4.5 35.5 88-89 28.249983185541247 33.0 27.5 35.0 2.0 35.0 90-91 27.662885865555793 32.5 26.5 34.0 2.0 35.0 92-93 27.06824699809366 32.0 25.5 34.0 2.0 35.0 94-95 26.288123895237515 32.0 25.0 34.0 2.0 35.0 96-97 25.441971989213524 31.5 23.5 34.0 2.0 35.0 98-99 24.23399762705951 31.0 10.0 34.0 2.0 35.0 100-101 20.425159894993705 27.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1919732744042797 1101 2 0.29721369731775127 1101 3 0.24705793750135996 1101 4 0.15299350249187427 1101 5 0.21682504457861285 1101 6 0.21264015186366692 1101 7 0.1576880158670093 1101 8 0.1573533481999334 1101 9 0.2794557332449301 1101 10-11 0.17501906676879742 1101 12-13 0.253396151921514 1101 14-15 0.27690050154981094 1101 16-17 0.25550259623276617 1101 18-19 0.1814629130619423 1101 20-21 0.10513053666893057 1101 22-23 0.15260993345654583 1101 24-25 0.17990994583512787 1101 26-27 0.10236398389161394 1101 28-29 0.07427714716028078 1101 30-31 0.2779136136353131 1101 32-33 0.21292858395882774 1101 34-35 0.23887189899082273 1101 36-37 0.11444334351071461 1101 38-39 0.12331067484305436 1101 40-41 0.15336819397503376 1101 42-43 0.20938278900565166 1101 44-45 0.248591994436822 1101 46-47 0.22427196203013722 1101 48-49 0.22564931527430332 1101 50-51 0.15090128724893503 1101 52-53 -0.021604454040904386 1101 54-55 0.08459360102968816 1101 56-57 0.0670554270317254 1101 58-59 0.059488626794504285 1101 60-61 -0.0045191775892874375 1101 62-63 0.16622931654064388 1101 64-65 0.13196120543378953 1101 66-67 0.005290811748658086 1101 68-69 -0.002178650019331485 1101 70-71 -0.09266929125574208 1101 72-73 -0.11586731338635659 1101 74-75 0.06503994690413961 1101 76-77 0.037011539574194785 1101 78-79 0.17864754401665905 1101 80-81 0.3259279127109451 1101 82-83 0.045297718233765494 1101 84-85 -0.03481420154529147 1101 86-87 -0.05681544924323845 1101 88-89 -0.24279375158944205 1101 90-91 -0.2184026864190578 1101 92-93 -0.18288527652784836 1101 94-95 -0.17046353059269492 1101 96-97 -0.17504969015279315 1101 98-99 -0.716608676837776 1101 100-101 -0.42975725687800903 1102 1 0.12120707736326253 1102 2 0.32537468570689043 1102 3 0.23585217967435312 1102 4 0.1508371546641385 1102 5 0.039313719023681415 1102 6 0.05218296829352198 1102 7 0.13906902424334788 1102 8 0.13026028791992417 1102 9 0.0920568120493499 1102 10-11 0.14750218483514743 1102 12-13 0.1620683980856441 1102 14-15 0.28694434571269767 1102 16-17 0.337900343101488 1102 18-19 0.20100580042429073 1102 20-21 0.1502652768826067 1102 22-23 0.1696615139901425 1102 24-25 0.19184236765963192 1102 26-27 0.23222739155169592 1102 28-29 0.28380168665076866 1102 30-31 0.31406081274755593 1102 32-33 0.35207685809242406 1102 34-35 0.2240736073605447 1102 36-37 0.20009058675872637 1102 38-39 0.16059574938523014 1102 40-41 0.18494221754983897 1102 42-43 0.24168662913326244 1102 44-45 0.36751150339480887 1102 46-47 0.12870047468607737 1102 48-49 0.36831406781447384 1102 50-51 0.3616697068414325 1102 52-53 0.3701125615610934 1102 54-55 0.36884177964085296 1102 56-57 0.44414340381630524 1102 58-59 0.3430460017247583 1102 60-61 0.314771229500181 1102 62-63 0.22852656782767866 1102 64-65 0.21207886667911424 1102 66-67 0.2503990310908506 1102 68-69 0.09916792457170942 1102 70-71 -0.04453233321940431 1102 72-73 -0.1973133826227773 1102 74-75 0.019144849758145455 1102 76-77 0.04223887033714391 1102 78-79 0.021711764720752313 1102 80-81 0.0880439708818983 1102 82-83 0.18122171643050677 1102 84-85 -0.2002285812958 1102 86-87 -0.0292437916611874 1102 88-89 -0.18438773872071224 1102 90-91 -0.27639650268183047 1102 92-93 -0.12832065229667933 1102 94-95 0.14407834007057474 1102 96-97 0.0842536975831365 1102 98-99 -0.029871792255978136 1102 100-101 -0.11920258053510224 1103 1 0.1728571173031348 1103 2 0.20017275328056172 1103 3 0.15412431014971162 1103 4 0.0863029965022406 1103 5 0.1279639682331819 1103 6 0.16520983221807484 1103 7 0.17454937033787132 1103 8 0.14593530580566494 1103 9 0.016334172563915672 1103 10-11 0.1504918800075714 1103 12-13 0.08067777379012142 1103 14-15 0.2041011690508583 1103 16-17 0.16977788081467793 1103 18-19 0.07640599667836767 1103 20-21 0.04639640471827988 1103 22-23 0.09893917437276656 1103 24-25 0.08852835773888756 1103 26-27 0.09189206462745858 1103 28-29 0.16543275834093407 1103 30-31 0.28971407850212216 1103 32-33 0.2260104124348956 1103 34-35 0.16259103203410774 1103 36-37 0.19809205122074758 1103 38-39 0.10689879094055499 1103 40-41 0.18563074438527138 1103 42-43 0.17380011622022096 1103 44-45 0.11007062844362281 1103 46-47 0.0953335072155923 1103 48-49 0.11051531841314244 1103 50-51 0.2510214771688766 1103 52-53 0.30137615664308015 1103 54-55 0.30548976091207436 1103 56-57 0.1635748858790933 1103 58-59 0.10746029773238774 1103 60-61 -0.0898689203232621 1103 62-63 0.03571895636820699 1103 64-65 0.05832253010550659 1103 66-67 -0.10478229550842144 1103 68-69 -0.04374350291083573 1103 70-71 -0.19647712644655257 1103 72-73 0.13775328378868323 1103 74-75 0.019333011647766796 1103 76-77 -0.1478673765312557 1103 78-79 -0.19281628974379572 1103 80-81 0.13364390691004502 1103 82-83 0.10833782789465474 1103 84-85 0.2457299310013461 1103 86-87 0.04126598191078301 1103 88-89 -0.18032479237455235 1103 90-91 0.0723898717389524 1103 92-93 -0.1147519948463831 1103 94-95 -0.24705977192610717 1103 96-97 -0.03732296825836556 1103 98-99 0.048258617597728204 1103 100-101 0.08241073088447237 1104 1 0.10375029184764983 1104 2 0.1624164121611429 1104 3 0.09523132332261497 1104 4 0.050402040903279044 1104 5 0.027335701510196486 1104 6 0.06208173938510697 1104 7 0.05630164536400173 1104 8 0.08108899524899726 1104 9 0.04607900258328357 1104 10-11 0.052587191848942894 1104 12-13 0.05884892011500398 1104 14-15 0.12597385139024908 1104 16-17 0.12648303793164217 1104 18-19 0.03910344564898338 1104 20-21 0.046117342668452466 1104 22-23 -0.011987152408053703 1104 24-25 -0.01923903691937312 1104 26-27 -9.270738334095086E-4 1104 28-29 0.10602522467121389 1104 30-31 0.05266218050307003 1104 32-33 0.20434287841321463 1104 34-35 0.1623517761325317 1104 36-37 0.13741155883126766 1104 38-39 0.246255087812294 1104 40-41 0.1854430269703684 1104 42-43 0.12619196291543489 1104 44-45 0.31475887088065946 1104 46-47 0.20635913986098586 1104 48-49 0.21636573277420013 1104 50-51 0.10384683376911852 1104 52-53 0.046810391439016996 1104 54-55 -0.1584127668202413 1104 56-57 -0.07218100773540925 1104 58-59 0.09213367362058023 1104 60-61 -0.07763376738803629 1104 62-63 -0.24176383690044645 1104 64-65 -0.2106011032656241 1104 66-67 -0.09030668494496297 1104 68-69 -0.13172011868429934 1104 70-71 -0.30631485982581097 1104 72-73 -0.07908547114204367 1104 74-75 -0.005211040349959006 1104 76-77 -0.0681683445329746 1104 78-79 -0.07955899872397865 1104 80-81 -0.11557819715076789 1104 82-83 -0.1434565913671655 1104 84-85 -0.23715426327612477 1104 86-87 -0.13218155735693315 1104 88-89 -0.1497582796529251 1104 90-91 -0.1656844157519437 1104 92-93 -0.20014037295953813 1104 94-95 -0.29308196448227264 1104 96-97 -0.03958647155138095 1104 98-99 0.03500652232778734 1104 100-101 0.09214312462510321 1105 1 0.1184688939286822 1105 2 0.27015070190682167 1105 3 0.07359110395943702 1105 4 0.16365683165332712 1105 5 0.1332706015247922 1105 6 0.09480953025331473 1105 7 0.1047760425670532 1105 8 0.10855485007727594 1105 9 0.1558553994944063 1105 10-11 0.19430776096989177 1105 12-13 0.12330228458734638 1105 14-15 0.2902713818168152 1105 16-17 0.2876031385773601 1105 18-19 0.20677789011659087 1105 20-21 0.13479261426592615 1105 22-23 0.12285816040689923 1105 24-25 0.2792570463774666 1105 26-27 0.2661332788436468 1105 28-29 0.24952537411063958 1105 30-31 0.24695408088604864 1105 32-33 0.27951598487663887 1105 34-35 0.038546492232377716 1105 36-37 0.34424935102010323 1105 38-39 0.35969573950721667 1105 40-41 0.4990210934326953 1105 42-43 0.4757094473285477 1105 44-45 0.4249582856341547 1105 46-47 0.4945535640326426 1105 48-49 0.43411406287547294 1105 50-51 0.7144056677245274 1105 52-53 0.7102040565973908 1105 54-55 0.6320612689479148 1105 56-57 0.7118572626595991 1105 58-59 0.7172441545375179 1105 60-61 0.6776543726818858 1105 62-63 0.5732297337287946 1105 64-65 0.6009432663432648 1105 66-67 0.5453408743268895 1105 68-69 0.6121225598263109 1105 70-71 0.5798484392407133 1105 72-73 0.7913959323374655 1105 74-75 0.7573970599621269 1105 76-77 0.47495063010402205 1105 78-79 0.5119120413049352 1105 80-81 0.8428782673208772 1105 82-83 0.6797824908662271 1105 84-85 0.734263812639103 1105 86-87 0.8487268953541758 1105 88-89 0.6832137577972937 1105 90-91 0.7921918066973355 1105 92-93 0.8875465132093225 1105 94-95 1.0660717218061357 1105 96-97 1.1072782197345674 1105 98-99 0.9882995417688143 1105 100-101 0.8021993555541975 1106 1 0.03706471935562661 1106 2 0.15761433252035317 1106 3 0.14610217290314154 1106 4 0.12079788162169791 1106 5 0.1842256932389006 1106 6 0.05474627914642127 1106 7 0.0643838857043022 1106 8 0.05682809485906404 1106 9 0.19033990297321424 1106 10-11 0.08824830492940805 1106 12-13 0.10237249331599685 1106 14-15 0.2155401990211132 1106 16-17 0.23084792036103607 1106 18-19 0.11681584078073115 1106 20-21 0.03934669396232238 1106 22-23 0.09804475117223888 1106 24-25 0.19197684397392578 1106 26-27 0.11607635284743623 1106 28-29 0.3219415663940097 1106 30-31 0.2469857064394958 1106 32-33 0.17897835046803579 1106 34-35 0.0965287819224443 1106 36-37 0.10677939529587377 1106 38-39 0.19104298808407094 1106 40-41 0.1397358309405945 1106 42-43 0.23485555738547959 1106 44-45 0.21600192889791003 1106 46-47 0.17919619527869202 1106 48-49 0.29312102049723165 1106 50-51 0.22582249251896513 1106 52-53 0.18523884470618412 1106 54-55 0.23307961176891467 1106 56-57 0.34102234804858966 1106 58-59 0.31477103625795166 1106 60-61 0.35366955294754376 1106 62-63 0.2933182852784455 1106 64-65 0.2796529437626205 1106 66-67 0.05721310709095917 1106 68-69 0.1996304662146784 1106 70-71 0.14823553601490502 1106 72-73 0.3577147179162097 1106 74-75 0.3511921263757891 1106 76-77 0.15873937140699823 1106 78-79 0.12311381233592655 1106 80-81 0.19200540204573358 1106 82-83 0.3097065895379494 1106 84-85 0.39067747487819204 1106 86-87 0.3409343589847893 1106 88-89 0.4749658134582653 1106 90-91 0.4382196371843712 1106 92-93 0.25655347083107927 1106 94-95 0.2586207414139814 1106 96-97 0.4188088965214085 1106 98-99 0.5472622236157463 1106 100-101 -0.16745276335788262 1107 1 0.05980767290284916 1107 2 0.08210176186191376 1107 3 0.04835374120549574 1107 4 0.06386157360420697 1107 5 -0.014890433854191087 1107 6 0.03228508675184116 1107 7 -0.010187800499480204 1107 8 0.014420229592474243 1107 9 -0.09067583337746044 1107 10-11 0.0785141649020602 1107 12-13 0.133926057992106 1107 14-15 0.06257375632838347 1107 16-17 0.0716421067345081 1107 18-19 0.07951768396019077 1107 20-21 0.06419907477619091 1107 22-23 0.061951496569136566 1107 24-25 0.0899331545123303 1107 26-27 0.1843830710959864 1107 28-29 0.14102734550334617 1107 30-31 0.08949673545915005 1107 32-33 0.13538914387874712 1107 34-35 0.08264133125003781 1107 36-37 0.22524813105510333 1107 38-39 0.2235409277177567 1107 40-41 0.16795247482203735 1107 42-43 0.24015065032012473 1107 44-45 0.18306486460478766 1107 46-47 0.20909448590183644 1107 48-49 0.17461195003755137 1107 50-51 0.1579276536153742 1107 52-53 0.10367034843422829 1107 54-55 0.12948148500547774 1107 56-57 0.20520969109715992 1107 58-59 0.1523989486463222 1107 60-61 0.08495047056197791 1107 62-63 -0.15750295249267054 1107 64-65 0.05303223538444257 1107 66-67 0.09191171185937463 1107 68-69 0.013070065970339328 1107 70-71 -0.155393520482761 1107 72-73 -0.0020917530883721724 1107 74-75 0.1674509859509179 1107 76-77 -0.09200652967603062 1107 78-79 0.08307454417804294 1107 80-81 0.05072564793227485 1107 82-83 0.2977624763680886 1107 84-85 0.10248885197855628 1107 86-87 0.41101813105494855 1107 88-89 0.058188032448541094 1107 90-91 0.20138503854372303 1107 92-93 0.09666177338012005 1107 94-95 0.42544069933402895 1107 96-97 0.4309131849920824 1107 98-99 0.5727886593274683 1107 100-101 0.32431343112130406 1108 1 -0.029729635483082717 1108 2 0.1572043862837944 1108 3 -0.03233868731191336 1108 4 0.07274008592277426 1108 5 0.1089501556044894 1108 6 -0.029037323004111215 1108 7 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-1.2971833835196485 2316 84-85 -1.269625445095592 2316 86-87 -1.5035167199248924 2316 88-89 -1.4801403784395966 2316 90-91 -2.2546204802942107 2316 92-93 -2.020358590141271 2316 94-95 -1.9230797852978334 2316 96-97 -1.6521078733031018 2316 98-99 -1.208631366034485 2316 100-101 -0.8018594237137151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 18.0 5 38.0 6 90.0 7 215.0 8 601.0 9 1098.0 10 1843.0 11 2792.0 12 4125.0 13 5684.0 14 7405.0 15 9504.0 16 11787.0 17 15038.0 18 14144.0 19 11448.0 20 11289.0 21 12146.0 22 13536.0 23 15177.0 24 17451.0 25 21098.0 26 25968.0 27 32209.0 28 39490.0 29 49014.0 30 60473.0 31 74384.0 32 93584.0 33 119569.0 34 159205.0 35 215926.0 36 305389.0 37 367451.0 38 177753.0 39 6172.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14499749066554 18.911658289080584 14.715262796948862 23.228081423305017 2 16.048589923203284 20.359852565443266 38.82905768214127 24.762499829212178 3 20.679966326958507 22.961619669091952 29.160233861585304 27.198180142364237 4 13.704727560102716 16.49015798818465 36.07681503554432 33.72829941616832 5 14.43315545956853 37.1019450509318 33.80426529202038 14.660634197479288 6 33.45110460484971 35.60004497867716 16.412435553332312 14.536414863140815 7 29.820978183193915 31.991729370717557 19.567342048846108 18.619950397242423 8 26.763047118806693 34.68319563571321 18.850149993457986 19.703607252022113 9 25.98232061965582 17.228541958183428 19.87120104291845 36.917936379242306 10-11 25.90217452987824 26.08679203246872 26.526411311086402 21.484622126566634 12-13 26.660296438907814 23.213542575260465 27.01718332594198 23.10897765988974 14-15 23.13142894375108 25.181623572338356 25.43862368583667 26.24832379807389 16-17 22.861595916607527 28.144867212761994 26.097548351296396 22.895988519334082 18-19 22.718200144869925 27.39250160200285 27.883467199205082 22.005831053922144 20-21 23.674177787271002 26.091482290974543 27.78959614294801 22.444743778806444 22-23 23.13850218431017 26.036246832829075 27.957094620512237 22.868156362348525 24-25 23.534337126237773 26.044458690094924 27.959240508324513 22.461963675342794 26-27 23.138570800280174 26.121118940939645 28.272033149757686 22.468277109022495 28-29 22.776655646190157 27.00302239896087 27.887489201964772 22.332832752884205 30-31 23.216368768627387 26.255102169499224 27.643486037605776 22.88504302426762 32-33 23.081134920445166 26.29046873756114 27.816985132091315 22.811411209902378 34-35 23.57699953076781 26.226007023244595 27.770558596486065 22.426434849501526 36-37 23.46859018736461 26.116259853949398 27.9719091012988 22.443240857387195 38-39 23.114015843197016 26.563418767488606 27.926065080595368 22.396500308719013 40-41 23.333331581826293 26.47795229210093 27.521368823770192 22.667347302302584 42-43 23.12358633655942 26.521239139636123 27.191285067851904 23.163889455952553 44-45 23.433070328244014 26.23390374696861 27.326044860628322 23.006981064159053 46-47 23.805201342634533 26.80250997833037 27.030109441108408 22.362179237926693 48-49 23.814640846672724 26.693905068276415 26.880287829210964 22.611166255839894 50-51 23.203919104108692 26.915886180775665 27.26573863110902 22.614456084006626 52-53 23.71476515621441 27.17841969349571 26.42473052597452 22.68208462431536 54-55 23.289979985508015 26.809593294383276 26.73432220021743 23.166104519891274 56-57 23.627534394081366 26.809410622159884 26.66452428987567 22.898530693883085 58-59 24.044781279951575 26.606481520404902 26.642738687763785 22.70599851187974 60-61 23.54874153852945 26.779286593331292 27.22922506014068 22.442746807998578 62-63 23.20258123773723 26.859474819743177 27.222825909901537 22.71511803261805 64-65 23.194964699984684 27.228002287912865 26.77241651469527 22.80461649740718 66-67 23.563174149443604 26.755346889339325 26.521438132933632 23.16004082828344 68-69 23.35307677715954 26.8135216667306 26.785619514481535 23.047782041628327 70-71 23.762016464408564 26.690844103091198 26.585695113120554 22.96144431937968 72-73 23.63669468397958 26.608582698453194 26.893520110748565 22.86120250681866 74-75 23.261115977800888 27.390185495325962 26.413653683552823 22.935044843320323 76-77 23.58796942779983 26.861231521282498 26.32613131774632 23.22466773317135 78-79 23.604977456410474 26.92027766976006 26.444445028323997 23.03029984550547 80-81 23.79465802304132 26.849265616760796 26.38275366587329 22.973322694324587 82-83 23.669111360654284 26.845278037742908 26.665118261217753 22.82049234038506 84-85 23.421812694965453 27.089166587627357 26.53156505977342 22.957455657633766 86-87 23.46197691609035 27.092473571416747 26.320706657373062 23.124842855119844 88-89 23.2978535017154 27.02562671664951 26.15689105873562 23.519628722899466 90-91 23.503559915277776 26.706866194638827 26.36670556293747 23.422868327145927 92-93 23.65806615865666 26.905201738370554 26.55641887888709 22.880313224085697 94-95 23.43768883564271 26.890011612571 26.557345607978522 23.114953943807766 96-97 23.0306953682924 26.916128822077003 26.86731426956037 23.18586154007023 98-99 23.119244747447386 27.225232955624733 26.147569345464778 23.5079529514631 100-101 23.0066216709374 26.98913518397515 26.17881270232117 23.82543044276628 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 83.0 1 61.0 2 66.5 3 80.0 4 58.5 5 65.0 6 152.0 7 328.0 8 520.0 9 662.5 10 682.5 11 638.0 12 679.5 13 781.0 14 911.5 15 1079.0 16 1224.0 17 1375.0 18 1521.5 19 1687.5 20 1933.0 21 2265.5 22 2695.0 23 3280.0 24 4171.0 25 5302.0 26 6740.0 27 8801.5 28 11123.0 29 13978.0 30 17477.0 31 21259.5 32 25225.5 33 28622.0 34 31723.0 35 35100.0 36 39555.0 37 43556.5 38 46221.5 39 49007.0 40 52291.5 41 55926.0 42 58437.5 43 61283.0 44 64249.5 45 67548.5 46 71153.0 47 78359.5 48 91122.5 49 90353.5 50 77808.5 51 72187.0 52 71272.5 53 70328.0 54 69418.0 55 65892.0 56 59579.5 57 53570.0 58 49617.5 59 44588.5 60 36940.5 61 30421.0 62 24861.0 63 19212.0 64 13800.5 65 9904.5 66 7472.0 67 6039.0 68 4839.5 69 3819.0 70 2998.0 71 2041.0 72 1454.5 73 1152.5 74 840.5 75 599.5 76 432.0 77 250.5 78 146.5 79 93.5 80 65.0 81 41.0 82 24.0 83 14.5 84 11.5 85 4.5 86 1.5 87 3.5 88 3.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012032847045174145 2 0.009353042681401738 3 0.0062003316651989045 4 3.6781628522366384E-4 5 0.004676521340700869 6 0.0 7 2.1018073441352218E-4 8 0.0021543525277386024 9 1.5763555081014163E-4 10-11 0.0 12-13 0.0 14-15 6.305422032405665E-4 16-17 0.005175700584932983 18-19 0.0019704443851267703 20-21 6.568147950422568E-4 22-23 3.1527110162028326E-4 24-25 4.7290665243042494E-4 26-27 1.5763555081014163E-4 28-29 0.0 30-31 6.305422032405665E-4 32-33 0.003441709526021426 34-35 7.881777540507082E-4 36-37 8.66995529455779E-4 38-39 0.005201973176734674 40-41 5.2545183603380545E-5 42-43 0.002390805853953815 44-45 0.006252876848802285 46-47 0.0 48-49 0.002679804363772408 50-51 0.0018916266097216998 52-53 9.7208589666254E-4 54-55 1.5763555081014163E-4 56-57 0.013293931451655278 58-59 0.002732349547375788 60-61 0.004518885789890727 62-63 3.1527110162028326E-4 64-65 0.004177342096468754 66-67 0.0016551732835064874 68-69 0.0023645332621521245 70-71 0.00577997019637186 72-73 0.003914616178451851 74-75 0.008669955294557791 76-77 0.0011822666310760622 78-79 0.007566506438886799 80-81 0.003389164342418045 82-83 0.00478161170790763 84-85 0.0032840739752112843 86-87 0.001234811814679443 88-89 0.003730708035840019 90-91 0.010482764128874418 92-93 0.004072251729261993 94-95 7.356325704473277E-4 96-97 1.5763555081014163E-4 98-99 0.001339902181886204 100-101 0.0028637125063842397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1903124.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.80037044133089 #Duplication Level Percentage of deduplicated Percentage of total 1 80.09675679366327 51.10202754584904 2 12.073886351666596 15.406368438057157 3 3.5810916594721487 6.854249233760504 4 1.4900408574145574 3.802606347030683 5 0.7813888454138345 2.492644889806324 6 0.48873750034054786 1.8708980142178218 7 0.292453476284465 1.3061048086662692 8 0.20891314114298803 1.0662988635987745 9 0.14132142337418255 0.8114723246312103 >10 0.7850514921344809 8.804884169886531 >50 0.0420101642949412 1.8074924834272452 >100 0.015891801143101052 1.8980434540216453 >500 0.0011463637056696996 0.52246959232237 >1k 0.0012282468275032462 1.7625316574114676 >5k 8.188312183354975E-5 0.49190817731301134 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9416 0.49476544880943124 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3681 0.1934188208440438 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3419 0.17965198273995808 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3083 0.1619968010492222 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2940 0.15448283979393881 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2633 0.138351468427701 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2348 0.12337609110073752 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2322 0.12200991632704963 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2232 0.11728084980274538 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1940 0.10193765619055827 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.2545183603380545E-5 7 0.0 0.0 0.0 0.0 5.2545183603380545E-5 8 0.0 0.0 0.0 0.0 5.2545183603380545E-5 9 0.0 0.0 0.0 5.2545183603380545E-5 5.2545183603380545E-5 10-11 0.0 0.0 0.0 5.2545183603380545E-5 5.2545183603380545E-5 12-13 0.0 0.0 0.0 5.2545183603380545E-5 2.1018073441352218E-4 14-15 5.2545183603380545E-5 0.0 0.0 5.2545183603380545E-5 2.88998509818593E-4 16-17 3.1527110162028326E-4 0.0 0.0 5.2545183603380545E-5 3.1527110162028326E-4 18-19 3.1527110162028326E-4 0.0 0.0 1.3136295900845137E-4 3.1527110162028326E-4 20-21 3.1527110162028326E-4 0.0 0.0 1.5763555081014163E-4 4.2036146882704436E-4 22-23 3.1527110162028326E-4 0.0 0.0 2.1018073441352218E-4 4.2036146882704436E-4 24-25 3.1527110162028326E-4 5.2545183603380545E-5 0.0 2.3645332621521247E-4 4.2036146882704436E-4 26-27 3.1527110162028326E-4 5.2545183603380545E-5 0.0 3.678162852236638E-4 4.2036146882704436E-4 28-29 3.1527110162028326E-4 5.2545183603380545E-5 0.0 0.0011297214474726817 4.2036146882704436E-4 30-31 3.1527110162028326E-4 5.2545183603380545E-5 0.0 0.0022594428949453634 4.2036146882704436E-4 32-33 3.1527110162028326E-4 5.2545183603380545E-5 0.0 0.004045979137460303 4.7290665243042494E-4 34-35 3.1527110162028326E-4 5.2545183603380545E-5 0.0 0.005490971686553267 5.254518360338055E-4 36-37 3.1527110162028326E-4 5.2545183603380545E-5 0.0 0.007566506438886799 5.254518360338055E-4 38-39 3.1527110162028326E-4 5.2545183603380545E-5 0.0 0.011481122617338649 5.254518360338055E-4 40-41 3.415436934219735E-4 5.2545183603380545E-5 0.0 0.016131371366237827 5.254518360338055E-4 42-43 3.6781628522366384E-4 5.2545183603380545E-5 0.0 0.022384248215040113 5.517244278354957E-4 44-45 3.6781628522366384E-4 5.2545183603380545E-5 0.0 0.027901492493395068 6.042696114388763E-4 46-47 3.6781628522366384E-4 5.2545183603380545E-5 0.0 0.04074878988442161 6.305422032405665E-4 48-49 3.6781628522366384E-4 5.2545183603380545E-5 0.0 0.0548308990901276 6.305422032405665E-4 50-51 3.940888770253541E-4 1.0509036720676109E-4 0.0 0.06181940850937721 6.305422032405665E-4 52-53 4.2036146882704436E-4 1.5763555081014163E-4 0.0 0.07309035039230234 6.830873868439471E-4 54-55 4.2036146882704436E-4 1.5763555081014163E-4 0.0 0.09618395858598809 7.356325704473277E-4 56-57 4.2036146882704436E-4 1.5763555081014163E-4 0.0 0.12915606129710938 7.619051622490179E-4 58-59 4.2036146882704436E-4 1.5763555081014163E-4 0.0 0.16570123649326055 7.881777540507082E-4 60-61 4.466340606287347E-4 1.5763555081014163E-4 0.0 0.2082628352119988 7.881777540507082E-4 62-63 4.7290665243042494E-4 1.5763555081014163E-4 0.0 0.25300505905027737 8.407229376540887E-4 64-65 4.7290665243042494E-4 1.5763555081014163E-4 0.0 0.30055845021133676 8.407229376540887E-4 66-67 5.254518360338055E-4 1.5763555081014163E-4 0.0 0.37033845403662613 8.407229376540887E-4 68-69 5.254518360338055E-4 1.5763555081014163E-4 0.0 0.4563811921871617 8.407229376540887E-4 70-71 5.77997019637186E-4 1.5763555081014163E-4 0.0 0.502752316717145 8.407229376540887E-4 72-73 7.881777540507082E-4 1.5763555081014163E-4 0.0 0.5673303473656998 9.195407130591596E-4 74-75 7.881777540507082E-4 1.5763555081014163E-4 0.0 0.6408673318186309 0.0010246310802659207 76-77 7.881777540507082E-4 1.5763555081014163E-4 0.0 0.718213842082807 0.0011034488556709915 78-79 7.881777540507082E-4 2.1018073441352218E-4 0.0 0.8094585534100773 0.0011034488556709915 80-81 7.881777540507082E-4 2.1018073441352218E-4 0.0 0.9436064071495078 0.0011034488556709915 82-83 7.881777540507082E-4 2.1018073441352218E-4 0.0 1.0957247136812946 0.0011822666310760622 84-85 7.881777540507082E-4 2.1018073441352218E-4 0.0 1.3062995369718422 0.0012085392228777525 86-87 7.881777540507082E-4 2.1018073441352218E-4 0.0 1.6049138153898537 0.0012348118146794428 88-89 7.881777540507082E-4 2.1018073441352218E-4 0.0 1.9311668603832437 0.001261084406481133 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5995 0.0 24.09529 1 TACATGG 5850 0.0 23.79404 2 TAAGGTG 875 0.0 23.341328 5 ACATGGG 5835 0.0 22.713577 3 GAGTACT 5235 0.0 20.052269 12-13 GTACGAT 145 6.1453413E-4 19.656467 3 GTAAGGT 720 0.0 19.131193 4 GTATACG 125 0.0060202107 19.006744 1 GTACTTT 5580 0.0 18.642231 14-15 AGAGTAC 8005 0.0 18.39403 10-11 CATGGGG 3950 0.0 18.037266 4 AGTACTT 5585 0.0 17.945156 12-13 GTACCGT 215 2.3981966E-5 17.67233 6 GTGTAGG 770 0.0 16.661757 1 GAGTACA 4870 0.0 16.586996 1 GTACCTG 1615 0.0 16.476435 1 AGTACAT 4490 0.0 16.188313 2 ACTTTTT 6585 0.0 16.013458 16-17 TACTTTT 6190 0.0 15.999388 14-15 GGACCAT 980 0.0 15.99301 6 >>END_MODULE