FastQCFastQC Report
Thu 26 May 2016
SRR936574_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936574_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195738
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT69200.31515599766456653No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53360.2430162432858565No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38010.17310808484436668No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37260.1696923767771929No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33180.1511109248917676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31660.14418842320896208No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24150.1099857997629954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG66250.022.2362611
GTCTACG2606.220944E-1021.9327891
TACATGG66100.021.5673052
GTGTAGC13650.020.888371
GAGTACT48450.020.44534112-13
GTACCTG17850.020.2330471
TATAGCG1656.303446E-520.1609025
ACATGGG67450.019.9379523
AGAGTAC81200.019.48564710-11
ATACCGT2451.7504863E-719.3954626
GTATAGG6500.019.0084171
GTATACG1250.006017440519.0084171
TAGCCCT15400.018.514274
GTACTTT52950.018.4373414-15
TATACCG1559.5982576E-418.3956625
TACCTGG17650.018.3079722
CATGGGG43650.018.2894694
AGTACTT53100.017.8496812-13
CGTCGTG2002.7548455E-416.630477
GTAGCCC12200.016.359333
Produced by FastQC (version 0.11.5)