##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936574_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2195738 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29440443258713 31.0 31.0 34.0 28.0 34.0 2 31.364559888292682 31.0 31.0 34.0 28.0 34.0 3 31.4083907096384 31.0 31.0 34.0 28.0 34.0 4 35.044117285395615 37.0 35.0 37.0 32.0 37.0 5 34.96364047076655 37.0 35.0 37.0 32.0 37.0 6 35.06388467112196 37.0 35.0 37.0 32.0 37.0 7 34.89789947616701 37.0 35.0 37.0 32.0 37.0 8 34.8787646795747 37.0 35.0 37.0 32.0 37.0 9 36.39655414261629 39.0 37.0 39.0 32.0 39.0 10-11 36.25772564850634 39.0 35.0 39.0 32.0 39.0 12-13 36.16872732539128 39.0 35.0 39.0 31.5 39.0 14-15 37.254079721715435 40.0 37.0 41.0 31.5 41.0 16-17 37.17995134210001 39.0 36.5 41.0 31.0 41.0 18-19 37.10480075491702 39.0 36.0 41.0 31.0 41.0 20-21 36.9794533774066 39.0 36.0 41.0 31.0 41.0 22-23 36.86297499974951 39.0 36.0 41.0 30.5 41.0 24-25 36.713573067460686 39.0 36.0 41.0 30.0 41.0 26-27 36.52093737959629 39.0 36.0 40.0 30.0 41.0 28-29 36.63852654551681 39.0 36.0 41.0 30.0 41.0 30-31 36.5950823823243 39.0 36.0 41.0 30.0 41.0 32-33 36.454229284186 39.0 35.5 41.0 30.0 41.0 34-35 36.29148650704228 39.0 35.0 40.0 30.0 41.0 36-37 36.07506952104486 39.0 35.0 40.0 29.0 41.0 38-39 35.90240297339665 38.0 35.0 40.0 28.5 41.0 40-41 35.76633050026916 38.0 35.0 40.0 27.5 41.0 42-43 35.52283970127584 38.0 34.5 40.0 27.0 41.0 44-45 35.28866695388976 38.0 34.0 40.0 26.5 41.0 46-47 35.01169470128039 38.0 34.0 40.0 25.5 41.0 48-49 34.823059718418136 38.0 34.0 40.0 24.5 41.0 50-51 34.15725919941268 37.5 33.0 39.5 24.0 40.5 52-53 34.36639753923282 38.0 33.5 39.5 24.5 40.5 54-55 34.74290511891674 38.0 34.0 40.0 25.5 41.0 56-57 34.65522002169658 38.0 34.0 40.0 25.0 41.0 58-59 34.403858520461 38.0 34.0 40.0 24.5 41.0 60-61 34.06601402353104 37.5 33.0 40.0 23.0 41.0 62-63 33.744652822877775 37.0 33.0 40.0 23.0 41.0 64-65 33.421340797490416 36.5 32.5 39.5 22.0 41.0 66-67 33.034286194436675 36.0 32.0 39.0 22.0 41.0 68-69 32.63004420381667 35.5 32.0 39.0 20.5 40.0 70-71 32.20474869952608 35.0 31.0 38.0 20.0 40.0 72-73 31.77194706290095 35.0 31.0 37.5 18.5 39.5 74-75 31.339097378649 35.0 31.0 37.0 18.0 39.0 76-77 30.92150793947183 35.0 30.0 36.0 15.0 39.0 78-79 30.4590668376646 34.0 30.0 36.0 11.0 38.5 80-81 30.034766215277052 34.0 29.5 35.5 7.5 37.0 82-83 29.624380048985806 34.0 29.0 35.0 7.0 37.0 84-85 29.23078527583892 34.0 29.0 35.0 2.0 36.0 86-87 28.900561906748436 34.0 29.0 35.0 2.0 36.0 88-89 28.514587122871674 34.0 28.5 35.0 2.0 36.0 90-91 28.147901981019594 34.0 27.5 35.0 2.0 35.0 92-93 27.75880296283072 33.5 27.0 35.0 2.0 35.0 94-95 27.31535319787698 33.0 26.0 35.0 2.0 35.0 96-97 26.70641829762932 33.0 25.0 35.0 2.0 35.0 98-99 26.03333184560271 33.0 24.5 35.0 2.0 35.0 100-101 23.959636122342467 31.0 11.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29695353847191797 1101 2 0.3725866764691297 1101 3 0.39242242945679706 1101 4 0.4398100741644839 1101 5 0.37079201159324526 1101 6 0.17075919560382857 1101 7 0.23696665896893165 1101 8 0.2324721208121474 1101 9 0.5791324817720493 1101 10-11 0.3673919651454156 1101 12-13 0.45394239308347295 1101 14-15 0.5237487623151793 1101 16-17 0.6109463672155258 1101 18-19 0.6359338066759719 1101 20-21 0.6125376031394723 1101 22-23 0.545119462994542 1101 24-25 0.5426649948935633 1101 26-27 0.482979844840024 1101 28-29 0.5392851978233466 1101 30-31 0.44993114047780836 1101 32-33 0.3944266042292739 1101 34-35 0.45836245841219636 1101 36-37 0.581585992016457 1101 38-39 0.5421159620671716 1101 40-41 0.4016231572227298 1101 42-43 0.2508733324246606 1101 44-45 0.3766109289786712 1101 46-47 0.5314037274734105 1101 48-49 0.5193248757517992 1101 50-51 0.7146145858717432 1101 52-53 0.6326647622513164 1101 54-55 0.6698075989542289 1101 56-57 0.5183623135885043 1101 58-59 0.5984733560640478 1101 60-61 0.4560467404434263 1101 62-63 0.5023908639693317 1101 64-65 0.7014807445475242 1101 66-67 0.6819723592607474 1101 68-69 0.5289858987558418 1101 70-71 0.37291237192346216 1101 72-73 0.41289714951340883 1101 74-75 0.404988255205712 1101 76-77 0.46895825385512424 1101 78-79 0.41997359485946717 1101 80-81 0.3668848678296186 1101 82-83 0.4306966984091254 1101 84-85 0.4566058097051382 1101 86-87 0.5356381784673552 1101 88-89 0.4860591266338936 1101 90-91 0.526672097334874 1101 92-93 0.17613607168520673 1101 94-95 0.1487294868403808 1101 96-97 0.1430100273232071 1101 98-99 -0.015313543749005731 1101 100-101 0.29237917929046375 1102 1 0.0962691036593526 1102 2 0.09803693250607637 1102 3 0.21495329107774452 1102 4 0.1462984026531231 1102 5 0.09577866850930405 1102 6 0.2407356705920165 1102 7 -0.0011118033735186827 1102 8 -0.03289246121678957 1102 9 0.23588491705879022 1102 10-11 0.301294306571414 1102 12-13 0.38474479801087114 1102 14-15 0.22890765214832953 1102 16-17 0.18299331594858614 1102 18-19 0.10051271898228009 1102 20-21 0.02815727599320894 1102 22-23 -0.2606882986453911 1102 24-25 -0.0633707818241902 1102 26-27 -0.12984991299205006 1102 28-29 -0.051029392859817335 1102 30-31 -0.14255062304656718 1102 32-33 -0.06467524343305797 1102 34-35 -0.13120469695361692 1102 36-37 -0.296133617892437 1102 38-39 -0.36405391333987325 1102 40-41 -0.5683888529441674 1102 42-43 -0.3439212786947081 1102 44-45 -0.29243425718726 1102 46-47 -0.049603262132059456 1102 48-49 -0.03578999164382424 1102 50-51 -0.05483904614631285 1102 52-53 -0.21181095055319332 1102 54-55 -0.31797533981896464 1102 56-57 -0.07508337992072 1102 58-59 -0.2859998856959365 1102 60-61 -0.6421456960454393 1102 62-63 -0.4513889158739559 1102 64-65 -0.18236165735228838 1102 66-67 -0.3270605885562432 1102 68-69 -0.3419976103611404 1102 70-71 -0.20262993608778856 1102 72-73 -0.27217532054605087 1102 74-75 -0.3181309265000998 1102 76-77 -0.39724873730866506 1102 78-79 -0.45801565943606093 1102 80-81 -0.43029246467780524 1102 82-83 -0.5490777464267573 1102 84-85 -0.5339800115081133 1102 86-87 -0.6939341026462422 1102 88-89 -0.8257638753401615 1102 90-91 -0.897352048777325 1102 92-93 -0.9578319694721529 1102 94-95 -0.7081342845857712 1102 96-97 -0.8500517081626064 1102 98-99 -0.8978273418056695 1102 100-101 -0.6912123058462605 1103 1 0.11342359291837667 1103 2 0.2722388240795368 1103 3 0.24479041839465765 1103 4 0.08462253635443773 1103 5 0.20312067808706757 1103 6 -0.041744045256940865 1103 7 0.011273114763589831 1103 8 -0.18760825253500713 1103 9 0.10698227867811028 1103 10-11 0.2051490734333754 1103 12-13 0.27297272754030644 1103 14-15 0.2959162499999124 1103 16-17 0.33097380650340114 1103 18-19 0.18351504425267962 1103 20-21 0.20652796698010434 1103 22-23 0.10443261656812552 1103 24-25 0.10470047151615347 1103 26-27 0.07969793316427598 1103 28-29 0.227346947023932 1103 30-31 0.13905697229873937 1103 32-33 0.09963790835163877 1103 34-35 0.1425837875972249 1103 36-37 0.22678735749912704 1103 38-39 0.09427221476788361 1103 40-41 0.15228213224649778 1103 42-43 0.1503040212156037 1103 44-45 0.34544465121529555 1103 46-47 0.34313672375723314 1103 48-49 0.5138586239153113 1103 50-51 -0.05318314120457046 1103 52-53 0.2825761787235379 1103 54-55 0.46705934901904556 1103 56-57 0.3976834612853324 1103 58-59 0.3278560801155592 1103 60-61 0.46697384268178155 1103 62-63 0.34294051395637126 1103 64-65 0.3399627253616231 1103 66-67 0.3236735951089855 1103 68-69 0.24575627296723468 1103 70-71 0.41689625404255537 1103 72-73 0.2259859058871001 1103 74-75 0.10268076233560564 1103 76-77 -0.05117829441209665 1103 78-79 0.11300466391140773 1103 80-81 0.3632064908526971 1103 82-83 0.33847260368956356 1103 84-85 0.10515606465285288 1103 86-87 0.045263534962035834 1103 88-89 -0.028967664481829303 1103 90-91 0.20698278785427604 1103 92-93 0.25881042999477444 1103 94-95 0.12595703847809503 1103 96-97 0.29716156997293197 1103 98-99 0.23744658934234408 1103 100-101 0.4534445582551143 1104 1 0.17746728056887662 1104 2 0.18855289356697824 1104 3 0.28569906255407673 1104 4 0.1694264579230662 1104 5 0.1576086136121546 1104 6 0.22267312511998227 1104 7 0.29984994900605955 1104 8 0.2860882436073098 1104 9 0.3747079654440455 1104 10-11 0.38406634209523105 1104 12-13 0.2854549133971034 1104 14-15 0.25591428430134755 1104 16-17 0.10710101177146925 1104 18-19 0.2946120269053907 1104 20-21 0.1859587743907909 1104 22-23 0.24553107349476022 1104 24-25 0.23358702613800375 1104 26-27 0.19140243952822544 1104 28-29 0.15842894122512519 1104 30-31 0.09001156466199944 1104 32-33 0.08900716482616389 1104 34-35 0.0524270231370636 1104 36-37 0.2105298018452828 1104 38-39 0.20497790055097198 1104 40-41 -0.013499970068110656 1104 42-43 -0.03065757394877977 1104 44-45 -0.023901508301143792 1104 46-47 0.272135077484684 1104 48-49 0.15383847550506857 1104 50-51 0.03709955081960459 1104 52-53 0.1961616128972068 1104 54-55 0.3895432548620832 1104 56-57 0.40607330845227096 1104 58-59 0.3044120533820589 1104 60-61 0.2267906641472166 1104 62-63 -0.10994292011901763 1104 64-65 0.09928627934462497 1104 66-67 -0.07569492422857849 1104 68-69 -0.011585611154842468 1104 70-71 0.08544691992293707 1104 72-73 -0.084831579001591 1104 74-75 -0.26160249482691 1104 76-77 -0.10092152503768048 1104 78-79 0.12090861496551142 1104 80-81 0.20560353739060844 1104 82-83 0.06893970280991724 1104 84-85 0.23517596735077362 1104 86-87 0.06887157466914928 1104 88-89 0.1295646958307195 1104 90-91 0.14464802938948296 1104 92-93 -0.06630014054923095 1104 94-95 0.05847706403217856 1104 96-97 0.1088445765088828 1104 98-99 0.12983995019546057 1104 100-101 0.327655207427064 1105 1 0.19133964216558397 1105 2 0.21531960998590804 1105 3 0.1455230894884494 1105 4 0.2553298703829441 1105 5 0.2894793667796023 1105 6 0.2764396438929353 1105 7 0.2466882950500775 1105 8 0.024834350322500143 1105 9 0.23592232953517822 1105 10-11 0.24167400803264627 1105 12-13 0.08368564121134625 1105 14-15 0.29518449360161725 1105 16-17 0.34815293132275116 1105 18-19 0.13291751941869734 1105 20-21 -0.16748759199031582 1105 22-23 -0.3343891184262944 1105 24-25 -0.29013355698575793 1105 26-27 -0.14313789728776527 1105 28-29 -0.04580971287398938 1105 30-31 -0.06810555079732694 1105 32-33 -0.2994848986658951 1105 34-35 -0.33656801526759494 1105 36-37 -0.3430656509343635 1105 38-39 -0.3564439918069482 1105 40-41 -0.5051758374953721 1105 42-43 -0.21102928467686866 1105 44-45 -0.2512226557382178 1105 46-47 -0.12139252820478674 1105 48-49 -0.14023555313315228 1105 50-51 -0.07861739289218406 1105 52-53 -0.21068937567340384 1105 54-55 -0.11548734618612144 1105 56-57 -0.03057468407807562 1105 58-59 -0.058871459536490534 1105 60-61 -0.09367847241252036 1105 62-63 -0.23914426002237832 1105 64-65 -0.24983521840289313 1105 66-67 -0.5491311356961219 1105 68-69 -0.204941639697644 1105 70-71 -0.10953730247648252 1105 72-73 -0.25751298733134576 1105 74-75 -0.152400371155057 1105 76-77 -0.044139712555995914 1105 78-79 -0.04499183280483621 1105 80-81 0.021070356721747174 1105 82-83 -0.06485094240193945 1105 84-85 -0.2816389337921663 1105 86-87 -0.12936784275231972 1105 88-89 -0.09491034966193368 1105 90-91 -0.07504756174234473 1105 92-93 -0.2104443675831149 1105 94-95 -0.3251387667283474 1105 96-97 -0.32399327882790274 1105 98-99 -0.3786545668327612 1105 100-101 0.02758177172675147 1106 1 0.09376515912439487 1106 2 0.1342132433679808 1106 3 0.1203067231229582 1106 4 0.31669888644754707 1106 5 0.2652071092487702 1106 6 0.3389034533779096 1106 7 0.3716559153404546 1106 8 0.3848431273112567 1106 9 0.45102884761441686 1106 10-11 0.43833661631291676 1106 12-13 0.44555642141185103 1106 14-15 0.3507950671545288 1106 16-17 0.3008690403981902 1106 18-19 0.37931827439506804 1106 20-21 0.42971950028262995 1106 22-23 0.3699240336583429 1106 24-25 0.12002037042543634 1106 26-27 0.1559622671836891 1106 28-29 0.04032089726266008 1106 30-31 0.14265150331902987 1106 32-33 0.05568934980443885 1106 34-35 0.22056610372273866 1106 36-37 0.21977506419603543 1106 38-39 0.2045086998353014 1106 40-41 0.0706412283106772 1106 42-43 0.08009935452093231 1106 44-45 0.058906028121249676 1106 46-47 0.23212613163446605 1106 48-49 -0.07470426375078887 1106 50-51 0.048097469540167026 1106 52-53 0.18002895871821778 1106 54-55 0.4243289195935205 1106 56-57 0.30967538242897064 1106 58-59 0.3109918175037194 1106 60-61 0.1851951991455678 1106 62-63 0.2317365263336768 1106 64-65 0.10341780159970426 1106 66-67 0.1865910143554288 1106 68-69 0.20975845719875963 1106 70-71 0.2020223024036767 1106 72-73 0.22951007944949708 1106 74-75 0.21490825845019046 1106 76-77 -0.008946317822577043 1106 78-79 0.15833604403769996 1106 80-81 0.06565027489380526 1106 82-83 0.2595556884965582 1106 84-85 0.1835164208506086 1106 86-87 0.18563226404732092 1106 88-89 0.2598034952611066 1106 90-91 0.3498192785222862 1106 92-93 0.2401573027632864 1106 94-95 0.19226316692771306 1106 96-97 0.305331961034387 1106 98-99 0.5123179507066489 1106 100-101 0.6264621231169905 1107 1 0.5865245997121917 1107 2 0.45813075062996944 1107 3 0.6151067613515835 1107 4 0.5307733917199116 1107 5 0.48616450460514926 1107 6 0.493991190967364 1107 7 0.7305694114929722 1107 8 0.7385221980081411 1107 9 0.9099217814007048 1107 10-11 0.8154683689505475 1107 12-13 0.789871506369181 1107 14-15 1.0262412849823903 1107 16-17 0.9122527718015547 1107 18-19 1.0086399190368383 1107 20-21 1.0613577416500632 1107 22-23 0.9435037099313988 1107 24-25 1.0159933323618944 1107 26-27 1.0934526738261994 1107 28-29 0.7629835209626634 1107 30-31 0.8424450108242922 1107 32-33 0.9584948095470907 1107 34-35 0.8830893087874472 1107 36-37 1.0410698561251621 1107 38-39 0.9293575954045465 1107 40-41 0.8751843528056753 1107 42-43 1.0671346378037327 1107 44-45 0.9070182001732263 1107 46-47 0.9377738316903574 1107 48-49 1.0519193667521876 1107 50-51 1.0016743076274608 1107 52-53 0.9664334696959074 1107 54-55 1.2036588181428627 1107 56-57 1.1431274561870524 1107 58-59 1.2954263958738466 1107 60-61 1.2628951681572147 1107 62-63 1.3532247782719011 1107 64-65 1.2252089864264164 1107 66-67 1.3374585434830308 1107 68-69 1.2767396411855927 1107 70-71 1.2740476375557677 1107 72-73 1.2820767550940992 1107 74-75 1.0535084536478685 1107 76-77 1.1639035392544947 1107 78-79 1.2339380724716875 1107 80-81 1.1813402291642596 1107 82-83 1.2538379026289839 1107 84-85 1.3674968894987956 1107 86-87 1.2756913736099804 1107 88-89 1.39375369958152 1107 90-91 1.3600428290926736 1107 92-93 1.5650342905793622 1107 94-95 1.5686763951902485 1107 96-97 1.617372819324931 1107 98-99 1.661850084472082 1107 100-101 1.8432085775568687 1108 1 0.14391150006674636 1108 2 0.10254894450488194 1108 3 -0.02695730655825912 1108 4 0.025269445213588426 1108 5 0.27407782717393303 1108 6 0.19469851908890945 1108 7 0.3435114543985307 1108 8 0.12322379891292456 1108 9 0.2628164146873502 1108 10-11 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-2.6220501662076288 2316 70-71 -2.041027690434028 2316 72-73 -2.1248511868447295 2316 74-75 -2.3932143600858318 2316 76-77 -3.242028111261913 2316 78-79 -3.2570204951400683 2316 80-81 -3.456331103132264 2316 82-83 -3.2092040880397583 2316 84-85 -3.290941679708048 2316 86-87 -3.580981115124718 2316 88-89 -3.820381005526748 2316 90-91 -3.74181401183726 2316 92-93 -4.002689280820725 2316 94-95 -4.04404681731523 2316 96-97 -4.218620481309117 2316 98-99 -4.532189712951851 2316 100-101 -5.18113564224614 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5475.0 3 2122.0 4 2141.0 5 2369.0 6 2866.0 7 3272.0 8 4099.0 9 4984.0 10 6137.0 11 7361.0 12 8746.0 13 9993.0 14 10971.0 15 12304.0 16 14420.0 17 16588.0 18 15754.0 19 13517.0 20 14000.0 21 15452.0 22 17302.0 23 20112.0 24 22804.0 25 26616.0 26 31355.0 27 36710.0 28 43894.0 29 52303.0 30 64010.0 31 77739.0 32 96801.0 33 122661.0 34 161172.0 35 218142.0 36 303607.0 37 390226.0 38 295120.0 39 42580.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10478499562963 17.898515441133934 14.262933580873682 23.73376598236275 2 15.536401128618307 19.152078811990318 40.19201996716167 25.119500092229703 3 20.206400059757453 22.503418290335595 29.09327172971412 28.196909920192827 4 13.0387539756049 15.533754998407709 36.82873725084273 34.598753775144665 5 14.485149651705747 36.422766524384244 34.155345432807096 14.936738391102914 6 33.31449796372946 34.6149131469209 17.202236730520088 14.868352158829559 7 29.55653804014824 31.40596278554882 20.18044612696829 18.85705304733465 8 26.39838262432687 34.21029254801812 19.488775538559587 19.902549289095425 9 25.673928144394466 17.069931105164265 20.289927688891417 36.96621306154985 10-11 25.60113872751119 25.615309032247925 26.997847799536206 21.785704440704677 12-13 26.367638857501113 22.842118164653392 27.38555965824178 23.404683319603713 14-15 23.06787931351774 24.651734517434456 25.702589340539006 26.577796828508802 16-17 22.769497360083037 27.66144650731731 26.38393214043388 23.185123992165778 18-19 22.588426469754154 26.99394761892071 27.9782368485251 22.439389062800032 20-21 23.430277608596523 25.80936337385028 27.88141742965261 22.87894158790058 22-23 22.953103865760262 25.70240120833619 27.98682071799165 23.357674207911895 24-25 23.183848731125607 25.74632805118628 27.99958463637351 23.0702385813146 26-27 22.879072942811767 25.780521651844374 28.34596951558037 22.994435889763484 28-29 22.55106460402815 26.54678532895447 28.01392631685855 22.888223750158833 30-31 22.87144101521802 25.88908435669268 27.791878028090434 23.44759659999886 32-33 22.738997022606558 25.938436839977914 28.01471048691484 23.30785565050069 34-35 23.3104833117722 25.974036916337862 27.691466185300367 23.02401358658957 36-37 23.081556425266474 25.72718606717644 28.17676639092084 23.01449111663625 38-39 22.899803949744275 26.14127141403268 27.99547096354294 22.963453672680103 40-41 23.057113045125778 26.09236936633718 27.803560928976808 23.046956659560234 42-43 22.830230140648467 26.07795383979533 27.41598722060734 23.675828798948864 44-45 22.98432832880975 25.919365163077966 27.52774895267529 23.568557555436993 46-47 23.415485821431513 26.273242873597297 27.403420272508704 22.907851032462485 48-49 23.287693068612512 26.185327650768176 27.297444758489465 23.22953452212985 50-51 22.814152307340922 26.413895168211848 27.650768081319 23.121184443128236 52-53 23.164420102171356 26.623850094023698 26.902277746327748 23.309452057477195 54-55 22.815688851755475 26.254362815456577 27.225152588951108 23.704795743836836 56-57 23.13236555946827 26.289024143641278 27.012736759071853 23.5658735378186 58-59 23.560334729405508 26.212537869748104 26.965241810752367 23.26188559009402 60-61 23.127973395539144 26.28634226008575 27.574369615096995 23.011314729278116 62-63 22.85574923333612 26.389337047234513 27.51994851789702 23.234965201532347 64-65 22.76472973948481 26.685540189696212 27.18122066240874 23.36850940841024 66-67 22.959257565710374 26.38669014289774 26.850438312043195 23.80361397934869 68-69 22.8380839991902 26.351558306607586 27.243226182128886 23.567131512073328 70-71 23.229184861734186 26.156397835516664 27.009037202996716 23.60538009975243 72-73 23.003135956976976 26.13162365874703 27.4138568262913 23.45138355798469 74-75 22.760700179991108 26.847937571504566 26.9617755722187 23.429586676285624 76-77 23.011826645761957 26.34995348816026 26.896527381639608 23.741692484438172 78-79 22.992118199894442 26.332743126574176 27.073383133025224 23.601755540506154 80-81 23.222706932244126 26.309451602477452 26.870437717216987 23.59740374806143 82-83 23.151336284636827 26.288371499881585 27.23083019074165 23.32946202473994 84-85 23.037077134586596 26.515465335562972 26.91306113237487 23.534396397475554 86-87 22.980654270521388 26.58230759596006 26.953121619621285 23.48391651389727 88-89 22.93274604234434 26.451322588902077 26.65306533285845 23.962866035895132 90-91 22.940992537668638 26.14895011076098 26.95498756885411 23.95506978271628 92-93 23.1624064962143 26.32148790728023 27.165239650957158 23.350865945548314 94-95 22.915565041111414 26.246425267771865 27.153732045496053 23.684277645620668 96-97 22.4907919912299 26.294210853039406 27.533301178743542 23.681695976987154 98-99 22.634250536619497 26.49809313309257 26.863796131966488 24.003860198321448 100-101 22.54694960284716 26.149870577337612 27.086835065727392 24.216344754087828 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 63.0 1 54.5 2 65.5 3 87.5 4 82.5 5 85.0 6 163.5 7 325.0 8 546.0 9 706.0 10 738.5 11 712.5 12 760.5 13 864.5 14 973.0 15 1090.0 16 1187.0 17 1346.0 18 1546.5 19 1711.0 20 1922.0 21 2253.5 22 2764.0 23 3500.0 24 4443.5 25 5653.5 26 7401.5 27 9755.5 28 12399.5 29 15646.0 30 19654.0 31 24134.5 32 29061.5 33 32978.0 34 36408.0 35 40368.5 36 45359.0 37 50008.5 38 53339.0 39 56625.5 40 60110.0 41 64228.5 42 67641.5 43 70524.0 44 74168.0 45 78395.5 46 81880.0 47 89631.0 48 103227.5 49 102850.0 50 90285.5 51 84573.0 52 83466.5 53 82288.0 54 80912.5 55 76444.5 56 69646.0 57 63217.5 58 57845.5 59 51526.5 60 43130.5 61 35694.0 62 29145.5 63 22621.5 64 16329.0 65 11667.0 66 8741.0 67 6978.5 68 5534.0 69 4380.5 70 3416.5 71 2397.5 72 1753.5 73 1339.0 74 966.5 75 674.5 76 494.5 77 307.5 78 174.0 79 113.0 80 72.0 81 53.0 82 36.0 83 19.0 84 17.0 85 14.5 86 7.5 87 7.5 88 8.5 89 5.5 90 3.5 91 5.0 92 4.5 93 3.0 94 2.5 95 3.0 96 2.5 97 1.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012342091816054557 2 0.00605718897245482 3 0.008926383748880787 4 0.035933248866668065 5 0.047455570746600916 6 0.058522464884243924 7 0.028373148344656783 8 0.026278180730123536 9 0.016532027045121047 10-11 0.045884345035700984 12-13 0.04417649100211409 14-15 0.01958339291846295 16-17 0.022703072953148325 18-19 0.02967111741018282 20-21 0.023909956470216393 22-23 0.014687544688847212 24-25 0.0032563083573723278 26-27 0.0026870236795100326 28-29 0.0017761681949303605 30-31 0.006854187521462032 32-33 0.0011613407428390818 34-35 0.001981110678960787 36-37 0.005328504584791081 38-39 0.005806703714195409 40-41 0.00341570806717377 42-43 7.059130005492458E-4 44-45 0.0011157979686100983 46-47 0.002960280324883934 48-49 7.286843876637377E-4 50-51 0.0017989395820448525 52-53 6.831416134347541E-4 54-55 0.0023454528727926555 56-57 0.0020949676145332456 58-59 0.0024593098083651147 60-61 7.286843876637377E-4 62-63 0.0010702551943811147 64-65 0.00316522280891436 66-67 0.0019127965176173115 68-69 0.0060799603595693115 70-71 0.009222411781369181 72-73 0.0034840222285172457 74-75 0.003370165292944787 76-77 0.002755337840853508 78-79 0.0339065954134783 80-81 0.022611987404690358 82-83 0.004463191874440393 84-85 0.00758287190912577 86-87 0.007423472199324327 88-89 0.005465132907478033 90-91 0.01113520829898649 92-93 0.013913317526954492 94-95 0.005419590133249049 96-97 0.004326563551753443 98-99 0.023408985953697573 100-101 0.04513288926092275 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2195738.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.07510457068561 #Duplication Level Percentage of deduplicated Percentage of total 1 80.18351895951 51.37767362176155 2 11.962093580558495 15.329447941172253 3 3.5744473170669506 6.870992568704144 4 1.5126448787423523 3.8769151513491313 5 0.7860457819972537 2.518298283941019 6 0.4709159782804019 1.8104394331394078 7 0.30750073842867376 1.3792199379266221 8 0.21333437640241407 1.0935537981205348 9 0.14731449573530184 0.8495272547115555 >10 0.7842373292655488 8.844638059721511 >50 0.03960597196549236 1.7539825470320962 >100 0.016352218863561342 1.9197500782912629 >500 7.811466077614312E-4 0.3693841332608165 >1k 0.0010651999196744258 1.4485063437081658 >5k 1.4202665595659008E-4 0.5576708471598694 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6920 0.31515599766456653 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5336 0.2430162432858565 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3801 0.17310808484436668 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3726 0.1696923767771929 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3318 0.1511109248917676 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3166 0.14418842320896208 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2415 0.1099857997629954 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.55427742289836E-5 2 0.0 0.0 0.0 0.0 4.55427742289836E-5 3 0.0 0.0 0.0 0.0 4.55427742289836E-5 4 0.0 0.0 0.0 0.0 4.55427742289836E-5 5 0.0 0.0 0.0 0.0 4.55427742289836E-5 6 9.10855484579672E-5 0.0 0.0 0.0 4.55427742289836E-5 7 9.10855484579672E-5 0.0 0.0 0.0 4.55427742289836E-5 8 9.10855484579672E-5 0.0 0.0 0.0 4.55427742289836E-5 9 9.10855484579672E-5 0.0 0.0 0.0 4.55427742289836E-5 10-11 1.1385693557245901E-4 0.0 0.0 0.0 4.55427742289836E-5 12-13 1.366283226869508E-4 0.0 0.0 0.0 1.366283226869508E-4 14-15 1.366283226869508E-4 0.0 0.0 4.55427742289836E-5 2.5048525825940984E-4 16-17 2.732566453739016E-4 0.0 0.0 4.55427742289836E-5 3.1879941960288524E-4 18-19 2.732566453739016E-4 0.0 0.0 6.83141613434754E-5 3.1879941960288524E-4 20-21 2.732566453739016E-4 2.27713871144918E-5 0.0 1.1385693557245901E-4 3.871135809463606E-4 22-23 2.732566453739016E-4 4.55427742289836E-5 0.0 1.5939970980144262E-4 4.098849680608524E-4 24-25 2.732566453739016E-4 4.55427742289836E-5 0.0 2.049424840304262E-4 4.098849680608524E-4 26-27 2.732566453739016E-4 4.55427742289836E-5 0.0 3.41570806717377E-4 4.5542774228983604E-4 28-29 2.732566453739016E-4 4.55427742289836E-5 0.0 8.880840974651804E-4 4.5542774228983604E-4 30-31 2.732566453739016E-4 4.55427742289836E-5 0.0 0.001730625420701377 4.5542774228983604E-4 32-33 2.732566453739016E-4 6.83141613434754E-5 0.0 0.0030969086475708847 4.5542774228983604E-4 34-35 2.732566453739016E-4 9.10855484579672E-5 0.0 0.004645362971356327 4.5542774228983604E-4 36-37 2.732566453739016E-4 9.10855484579672E-5 0.0 0.0069452730699199995 4.5542774228983604E-4 38-39 2.732566453739016E-4 9.10855484579672E-5 0.0 0.01049760945978072 4.5542774228983604E-4 40-41 2.732566453739016E-4 9.10855484579672E-5 0.0 0.014049945849641442 4.5542774228983604E-4 42-43 2.732566453739016E-4 9.10855484579672E-5 0.0 0.01958339291846295 4.5542774228983604E-4 44-45 2.960280324883934E-4 9.10855484579672E-5 0.0 0.024456469760964196 4.7819912940432786E-4 46-47 3.1879941960288524E-4 9.10855484579672E-5 0.0 0.03515902170477534 5.009705165188197E-4 48-49 3.1879941960288524E-4 9.10855484579672E-5 0.0 0.046886286068738625 5.009705165188197E-4 50-51 3.1879941960288524E-4 9.10855484579672E-5 0.0 0.053057331976765904 5.009705165188197E-4 52-53 3.1879941960288524E-4 9.10855484579672E-5 0.0 0.06298565675868432 5.465132907478032E-4 54-55 3.1879941960288524E-4 9.10855484579672E-5 0.0 0.08063348177241547 5.920560649767868E-4 56-57 3.1879941960288524E-4 9.10855484579672E-5 0.0 0.10618297811487527 5.920560649767868E-4 58-59 3.1879941960288524E-4 9.10855484579672E-5 0.0 0.136081809396203 5.920560649767868E-4 60-61 3.643421938318688E-4 9.10855484579672E-5 0.0 0.17372291229645798 5.920560649767868E-4 62-63 4.5542774228983604E-4 9.10855484579672E-5 0.0 0.20756119354859276 6.83141613434754E-4 64-65 4.5542774228983604E-4 9.10855484579672E-5 0.0 0.24413204125446664 6.83141613434754E-4 66-67 4.5542774228983604E-4 9.10855484579672E-5 0.0 0.304157417688267 6.83141613434754E-4 68-69 4.5542774228983604E-4 9.10855484579672E-5 0.0 0.3760922295829466 6.83141613434754E-4 70-71 4.7819912940432786E-4 9.10855484579672E-5 0.0 0.413847189418774 7.059130005492458E-4 72-73 5.465132907478032E-4 9.10855484579672E-5 0.0 0.4667906644599674 7.514557747782295E-4 74-75 5.465132907478032E-4 9.10855484579672E-5 0.0 0.5262239848287911 7.742271618927213E-4 76-77 5.465132907478032E-4 9.10855484579672E-5 0.0 0.5884126430384682 7.742271618927213E-4 78-79 5.465132907478032E-4 9.10855484579672E-5 0.0 0.6678164699067011 7.742271618927213E-4 80-81 5.465132907478032E-4 9.10855484579672E-5 0.0 0.7811496635755268 7.742271618927213E-4 82-83 5.465132907478032E-4 9.10855484579672E-5 0.0 0.9050487808654766 8.197699361217048E-4 84-85 5.465132907478032E-4 9.10855484579672E-5 0.0 1.090453414751669 8.197699361217048E-4 86-87 5.465132907478032E-4 1.1385693557245901E-4 0.0 1.355398503828781 8.653127103506885E-4 88-89 6.148274520912787E-4 1.366283226869508E-4 0.0 1.6522918490275251 9.108554845796721E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6625 0.0 22.236261 1 GTCTACG 260 6.220944E-10 21.932789 1 TACATGG 6610 0.0 21.567305 2 GTGTAGC 1365 0.0 20.88837 1 GAGTACT 4845 0.0 20.445341 12-13 GTACCTG 1785 0.0 20.233047 1 TATAGCG 165 6.303446E-5 20.160902 5 ACATGGG 6745 0.0 19.937952 3 AGAGTAC 8120 0.0 19.485647 10-11 ATACCGT 245 1.7504863E-7 19.395462 6 GTATAGG 650 0.0 19.008417 1 GTATACG 125 0.0060174405 19.008417 1 TAGCCCT 1540 0.0 18.51427 4 GTACTTT 5295 0.0 18.43734 14-15 TATACCG 155 9.5982576E-4 18.395662 5 TACCTGG 1765 0.0 18.307972 2 CATGGGG 4365 0.0 18.289469 4 AGTACTT 5310 0.0 17.84968 12-13 CGTCGTG 200 2.7548455E-4 16.63047 7 GTAGCCC 1220 0.0 16.35933 3 >>END_MODULE