FastQCFastQC Report
Thu 26 May 2016
SRR936574_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936574_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195738
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT98120.44686570073478715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42680.194376560409302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39070.17793561891263895No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT35100.15985513754373246No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34180.15566520231466596No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT27190.12383080312860642No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26040.11859338409227331No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC24310.11071448415065914No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24020.1093937436980186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23290.10606912117930281No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG69100.023.7087421
GTATAGG6200.023.2467251
TACATGG68600.022.7818682
ACATGGG69400.022.037113
GAGTACT61200.020.44646312-13
GTACTTT63050.019.84652514-15
AGAGTAC94900.019.76604810-11
CATGGGG45600.019.1646674
AGTACTT64550.018.53929712-13
CGTATAC1350.00940746317.591163
TATAGGG7800.017.0522232
TACTTTT71700.016.55807314-15
TAGCCCT11850.016.4328864
ACTTTTT77250.016.41389516-17
GTGTAGC12100.015.8820591
GTAGCCC10500.015.8320433
AACCGAT1800.002623442315.829887
GAGTACA56000.015.3566761
GGACCGT3102.0694788E-615.3192386
TACACTG19950.015.2347725
Produced by FastQC (version 0.11.5)