FastQCFastQC Report
Thu 26 May 2016
SRR936573_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936573_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260888
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT71930.318149328936241No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT53300.235748077746443No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT38920.1721447502043445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37080.16400635502510516No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34700.15347951778239347No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31610.13981232153030138No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24780.10960295246823372No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG67150.023.4224151
TACATGG68700.022.4107912
ACATGGG69150.021.7839183
GTGTAGC14350.019.8677671
GTCTACG2902.4319888E-919.6622371
GAGTACT53400.019.62428912-13
GTACTTT54650.019.13152114-15
GGACCGT3357.2759576E-1018.4406876
AGAGTAC85600.018.31951110-11
GAGTACA54400.018.1682931
GTATATA12050.018.1392971
AGTACAT49800.017.5572852
TACCTGG16550.017.514622
CATGGGG47250.017.4004824
GTACCTG19250.017.2789341
AGTACTT56150.017.05500612-13
GTATAGG5750.016.5276781
TAGCCCT14700.016.488074
GTAGCCC13150.016.2613143
GTATAGC8300.016.0298581
Produced by FastQC (version 0.11.5)