##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936573_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2260888 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.314632569149822 31.0 31.0 34.0 28.0 34.0 2 31.38544324177049 31.0 31.0 34.0 28.0 34.0 3 31.43724589630269 31.0 31.0 34.0 28.0 34.0 4 35.05686880553128 37.0 35.0 37.0 32.0 37.0 5 35.0185918099437 37.0 35.0 37.0 32.0 37.0 6 35.117586541217435 37.0 35.0 37.0 32.0 37.0 7 34.979597839433005 37.0 35.0 37.0 32.0 37.0 8 34.9326733566634 37.0 35.0 37.0 32.0 37.0 9 36.42796547197384 39.0 37.0 39.0 32.0 39.0 10-11 36.32056364578874 39.0 36.0 39.0 32.0 39.0 12-13 36.213569845122805 39.0 35.0 39.0 32.0 39.0 14-15 37.275413908163515 40.0 37.0 41.0 31.5 41.0 16-17 37.13823064211938 39.0 36.0 41.0 31.0 41.0 18-19 37.05964470597394 39.0 36.0 41.0 31.0 41.0 20-21 36.94824666237337 39.0 36.0 41.0 30.5 41.0 22-23 36.79435978252793 39.0 36.0 41.0 30.0 41.0 24-25 36.9364044127794 39.0 36.0 41.0 30.5 41.0 26-27 36.909929195961936 39.0 36.0 41.0 30.0 41.0 28-29 36.813928642197226 39.0 36.0 41.0 30.0 41.0 30-31 36.653204404641 39.0 36.0 41.0 30.0 41.0 32-33 36.490103888383686 39.0 35.5 41.0 30.0 41.0 34-35 36.26854116612587 39.0 35.0 40.0 30.0 41.0 36-37 36.098663445513445 39.0 35.0 40.0 29.0 41.0 38-39 35.90191951127167 38.0 35.0 40.0 28.5 41.0 40-41 35.73522726468538 38.0 35.0 40.0 27.5 41.0 42-43 35.525977845872944 38.0 34.5 40.0 27.0 41.0 44-45 35.2911975294663 38.0 34.0 40.0 26.5 41.0 46-47 35.096004976805574 38.0 34.0 40.0 26.0 41.0 48-49 35.053096172831204 38.0 34.0 40.0 26.0 41.0 50-51 34.153239125511746 37.5 33.0 39.5 24.5 40.5 52-53 34.32211303700139 37.5 33.5 39.5 24.5 40.5 54-55 34.75298356221096 38.0 34.0 40.0 26.0 41.0 56-57 34.644974452516 38.0 34.0 40.0 25.0 41.0 58-59 34.37558428369738 38.0 33.5 40.0 24.5 41.0 60-61 34.026428332584366 37.5 33.0 40.0 23.0 41.0 62-63 33.69777671428218 37.0 33.0 40.0 23.0 41.0 64-65 33.34227524760183 36.5 32.5 39.0 22.0 41.0 66-67 32.93522722045497 36.0 32.0 39.0 21.5 41.0 68-69 32.50675155071812 35.5 31.5 39.0 20.5 40.0 70-71 32.04670576339916 35.0 31.0 38.0 19.0 40.0 72-73 31.629339445386062 35.0 31.0 37.0 18.0 39.5 74-75 31.188182696356478 35.0 30.5 37.0 16.5 39.0 76-77 30.74097191015212 34.5 30.0 36.0 12.5 39.0 78-79 30.286004879498677 34.0 30.0 36.0 9.0 38.5 80-81 29.797342238978665 34.0 29.0 35.0 7.0 37.0 82-83 29.2980278103117 34.0 29.0 35.0 4.0 37.0 84-85 28.96001372027274 34.0 29.0 35.0 2.0 36.0 86-87 28.6411372434194 34.0 29.0 35.0 2.0 36.0 88-89 28.2229891529346 34.0 27.5 35.0 2.0 35.5 90-91 27.789713378106303 33.0 27.0 35.0 2.0 35.0 92-93 27.393953172381824 33.0 26.0 35.0 2.0 35.0 94-95 26.90789216449466 33.0 25.0 35.0 2.0 35.0 96-97 26.28992347254707 33.0 24.5 35.0 2.0 35.0 98-99 25.551748251129645 32.5 22.0 35.0 2.0 35.0 100-101 23.072364486874186 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3481607282136956 1101 2 0.3091668321305434 1101 3 0.3679598495230003 1101 4 0.400877370638824 1101 5 0.43956214839577257 1101 6 0.21400488959767472 1101 7 0.3778238649406873 1101 8 0.42380898183069604 1101 9 0.593526985641617 1101 10-11 0.4508658284260676 1101 12-13 0.5728518937003102 1101 14-15 0.7303980443790579 1101 16-17 0.6697753107338471 1101 18-19 0.7495186408023073 1101 20-21 0.7341123793135722 1101 22-23 0.649027351571398 1101 24-25 0.6461907932293443 1101 26-27 0.5855081922060634 1101 28-29 0.5821814478009486 1101 30-31 0.5482440219522502 1101 32-33 0.4591825607564033 1101 34-35 0.431814730386229 1101 36-37 0.322130163281237 1101 38-39 0.30353370484800024 1101 40-41 0.34261073782984397 1101 42-43 0.1731474869074816 1101 44-45 0.22676010158636473 1101 46-47 0.3006083967049804 1101 48-49 0.35411318130098124 1101 50-51 0.2704371663790326 1101 52-53 0.42597713079803157 1101 54-55 0.39105197180837337 1101 56-57 0.20228632459172502 1101 58-59 0.2124476287264585 1101 60-61 0.09700506764654193 1101 62-63 0.10606524793714556 1101 64-65 0.2869844666503596 1101 66-67 0.2528810829892265 1101 68-69 0.31279405764645674 1101 70-71 0.27801241073120764 1101 72-73 0.20155118506484726 1101 74-75 0.022991859397141212 1101 76-77 0.16111937233287676 1101 78-79 0.053139123539654776 1101 80-81 0.19908689904981358 1101 82-83 0.26834201223664067 1101 84-85 0.35957419207375096 1101 86-87 0.3070802065506051 1101 88-89 0.36993443738785103 1101 90-91 0.2564114621935474 1101 92-93 0.1858731114852752 1101 94-95 0.4525986993090676 1101 96-97 0.2535318869888137 1101 98-99 0.24508215538520517 1101 100-101 0.6872843292903923 1102 1 0.3627744896813212 1102 2 0.40492617628510885 1102 3 0.2681241069946658 1102 4 0.2868503975632066 1102 5 0.3245673972889733 1102 6 0.32020735308960724 1102 7 0.32525293227771357 1102 8 0.2753448845734141 1102 9 0.5680116986494426 1102 10-11 0.4569734502403904 1102 12-13 0.4049187731699462 1102 14-15 0.356552281262303 1102 16-17 0.2877102677362018 1102 18-19 0.43915501650062794 1102 20-21 0.527903623413728 1102 22-23 0.3515950526131135 1102 24-25 0.21998749834233422 1102 26-27 0.20666338149222696 1102 28-29 0.3457376718892533 1102 30-31 0.24409749597356978 1102 32-33 0.1206632582968723 1102 34-35 -0.10494916110076957 1102 36-37 0.015660571413647517 1102 38-39 0.12031757828917478 1102 40-41 0.01638811016015751 1102 42-43 0.08565753411762955 1102 44-45 -0.26392585344598274 1102 46-47 -0.2159225561050917 1102 48-49 -0.08715089226723904 1102 50-51 -0.05085602879466222 1102 52-53 0.031099065420200134 1102 54-55 0.12831788925142007 1102 56-57 0.20960060815838233 1102 58-59 0.2750986571934817 1102 60-61 0.1596027102998434 1102 62-63 0.2813703087129866 1102 64-65 0.1993997006553343 1102 66-67 0.2952664294802432 1102 68-69 0.12939100955851757 1102 70-71 0.03741749630653146 1102 72-73 0.12838272420775 1102 74-75 0.024248632633277367 1102 76-77 0.16721688643313826 1102 78-79 -0.08439497575909627 1102 80-81 0.24300064579719205 1102 82-83 0.13074883263736226 1102 84-85 0.0030645822001957868 1102 86-87 0.12160991739310845 1102 88-89 0.03920490814253341 1102 90-91 0.11958803484708724 1102 92-93 0.0145445108399187 1102 94-95 0.05038841273117356 1102 96-97 -0.07570767978602078 1102 98-99 0.03209340114245762 1102 100-101 0.11035380573073539 1103 1 0.07912074491725463 1103 2 0.2419375581153318 1103 3 0.19301109520701232 1103 4 0.26135342213540014 1103 5 0.39551902180314613 1103 6 0.26443843690271507 1103 7 0.16117452196071014 1103 8 0.1455481889463215 1103 9 0.3759136036767927 1103 10-11 0.27754589890417236 1103 12-13 0.35225622058263184 1103 14-15 0.2324187144672223 1103 16-17 0.3661651852807708 1103 18-19 0.2840212234254835 1103 20-21 0.27789934860143717 1103 22-23 0.3524973229944308 1103 24-25 0.36982337404903376 1103 26-27 0.24792443971916356 1103 28-29 -0.07528730840385833 1103 30-31 -0.02281993118219816 1103 32-33 0.2377071913977602 1103 34-35 0.1115397303720087 1103 36-37 0.10680655169365849 1103 38-39 0.2197120938759909 1103 40-41 -0.033535285203548426 1103 42-43 0.11673499200645665 1103 44-45 0.1259753584918002 1103 46-47 0.3030560430446201 1103 48-49 0.09902701516084988 1103 50-51 0.2160696629982084 1103 52-53 0.19737430177457327 1103 54-55 0.2817564342649064 1103 56-57 0.28757902662601964 1103 58-59 0.10726852911440687 1103 60-61 -0.035850114866605054 1103 62-63 0.10506622022550971 1103 64-65 0.14426459663774693 1103 66-67 -0.05981828874978845 1103 68-69 0.07199367487548614 1103 70-71 0.1747700651552364 1103 72-73 0.22245609594599713 1103 74-75 0.030859344856903448 1103 76-77 -0.003194515802189102 1103 78-79 0.09241468052889701 1103 80-81 0.012382074758647832 1103 82-83 -0.03922340388172785 1103 84-85 0.021595038527856047 1103 86-87 0.1170801837937141 1103 88-89 0.05607801974420212 1103 90-91 0.18467418941403935 1103 92-93 -0.0327988928112255 1103 94-95 -0.08850850330524551 1103 96-97 -0.1878920062722429 1103 98-99 -0.1780882897794953 1103 100-101 0.19568148149248898 1104 1 0.12248727428220363 1104 2 0.1881575034075169 1104 3 0.16251201942143112 1104 4 0.28741473131498196 1104 5 0.3069570474020509 1104 6 0.2386367119389945 1104 7 0.37686594432847187 1104 8 0.3209248225825192 1104 9 0.34743147950921127 1104 10-11 0.37970263573544827 1104 12-13 0.12029407139089443 1104 14-15 0.3247431817282731 1104 16-17 0.17913002728918315 1104 18-19 0.38429493990275176 1104 20-21 0.4575393129810408 1104 22-23 0.26883049194413644 1104 24-25 0.35600066238146155 1104 26-27 -0.049223504362025494 1104 28-29 0.2887115539195264 1104 30-31 0.34347202359929696 1104 32-33 -0.047621983857652594 1104 34-35 0.06405444507181102 1104 36-37 0.05211795223032567 1104 38-39 0.15350717963339378 1104 40-41 0.18577464652402398 1104 42-43 0.18587515920470565 1104 44-45 0.28896793284583566 1104 46-47 0.3845007385978647 1104 48-49 0.4384389948245868 1104 50-51 0.2715396759951574 1104 52-53 0.3718069121747334 1104 54-55 0.29469691438335843 1104 56-57 0.35352258272873627 1104 58-59 0.1693044262409984 1104 60-61 0.2804136345228656 1104 62-63 0.5009927584837257 1104 64-65 0.33032527903084485 1104 66-67 0.29289752980380257 1104 68-69 0.16894897080739923 1104 70-71 0.3110863700005382 1104 72-73 0.19744081063397445 1104 74-75 0.06043657145677983 1104 76-77 0.253608778492989 1104 78-79 0.07586042151470096 1104 80-81 0.2556133719819833 1104 82-83 0.35722525934825455 1104 84-85 0.40026720908947055 1104 86-87 0.5500926734014726 1104 88-89 0.4408252887881652 1104 90-91 0.4701905136542379 1104 92-93 0.41646096695416546 1104 94-95 0.49569109626096974 1104 96-97 0.6587333397907678 1104 98-99 0.8202383085556164 1104 100-101 1.1519495237781108 1105 1 0.19398665406372473 1105 2 0.21320051855995104 1105 3 0.15376958498968918 1105 4 0.17973271343427655 1105 5 0.275126264859729 1105 6 0.2536267234742411 1105 7 0.18862458102672974 1105 8 0.2897026415217496 1105 9 0.33623205054907146 1105 10-11 0.28307440411320783 1105 12-13 0.11552981249711536 1105 14-15 -0.02142316676179945 1105 16-17 0.12056229956058218 1105 18-19 -0.029260738420063603 1105 20-21 0.09882187660237918 1105 22-23 0.08592154466334279 1105 24-25 0.1648103032829269 1105 26-27 0.13385647090668584 1105 28-29 -0.04071534957895295 1105 30-31 -0.09989964151628783 1105 32-33 -0.12588675755642953 1105 34-35 -0.13694549856425198 1105 36-37 -0.04870842488917759 1105 38-39 -0.17610774708703048 1105 40-41 -0.29516638173495835 1105 42-43 0.019245494819699616 1105 44-45 -0.28702614851013664 1105 46-47 -0.24255552337140784 1105 48-49 -0.12881286722919327 1105 50-51 0.015656635904910843 1105 52-53 -0.06738930361052553 1105 54-55 -0.22485943686466925 1105 56-57 0.04234802274395122 1105 58-59 -0.055331262631767686 1105 60-61 -0.3609279821584508 1105 62-63 -0.11740281828073051 1105 64-65 -0.03154186900969336 1105 66-67 0.008866940103608556 1105 68-69 -0.02075914572913007 1105 70-71 0.053479819564053344 1105 72-73 0.09230087101653339 1105 74-75 0.0461269071061956 1105 76-77 -0.17723438733618124 1105 78-79 -0.014241962938147168 1105 80-81 -0.16507600203836859 1105 82-83 -0.013407449550062012 1105 84-85 0.022419808220085713 1105 86-87 0.18048075419208587 1105 88-89 -0.09026856749927603 1105 90-91 0.010415435895666292 1105 92-93 0.009840312194487666 1105 94-95 0.0086573841705615 1105 96-97 0.36706715716557525 1105 98-99 0.20976519996381526 1105 100-101 0.8148586302547578 1106 1 0.15480544501908255 1106 2 0.3600960686792192 1106 3 0.36652101155331707 1106 4 0.31346043940101964 1106 5 0.26582497174943853 1106 6 0.2918406383498322 1106 7 0.440470918574853 1106 8 0.3987022567709886 1106 9 0.6122153885983508 1106 10-11 0.5312373892752333 1106 12-13 0.5664122952003368 1106 14-15 0.7567219061984858 1106 16-17 0.7715348975958065 1106 18-19 0.6868637454608404 1106 20-21 0.5672398101707543 1106 22-23 0.5530295744647802 1106 24-25 0.5352362856257145 1106 26-27 0.28056512588401716 1106 28-29 0.5394965556226907 1106 30-31 0.5803180462852922 1106 32-33 0.2980425102857893 1106 34-35 0.43233464711190805 1106 36-37 0.34549009038104117 1106 38-39 0.40665965154676087 1106 40-41 0.34984257940521246 1106 42-43 0.24618659015751376 1106 44-45 0.5815846139792029 1106 46-47 0.49383117811012767 1106 48-49 0.6212390375894685 1106 50-51 0.48692133460625797 1106 52-53 0.5681684451615894 1106 54-55 0.6433284021386712 1106 56-57 0.5783777778111556 1106 58-59 0.6347547028343996 1106 60-61 0.6990039419002656 1106 62-63 0.8042231732335878 1106 64-65 0.7413562818937436 1106 66-67 0.9032678463718113 1106 68-69 0.7808139608221367 1106 70-71 0.8826334711814354 1106 72-73 0.9787158681831833 1106 74-75 0.7586553445217099 1106 76-77 0.9051594729788839 1106 78-79 0.9366542518651606 1106 80-81 0.8883040095167516 1106 82-83 1.0726060789621847 1106 84-85 0.8975293815599592 1106 86-87 0.984706927675628 1106 88-89 1.2009322681179135 1106 90-91 1.209286606875935 1106 92-93 1.4331056518951506 1106 94-95 1.3720806253845979 1106 96-97 1.5117515242152884 1106 98-99 1.9085598995243664 1106 100-101 2.228641954849099 1107 1 0.06915480878763702 1107 2 0.16427731609583773 1107 3 -0.03229078190551249 1107 4 0.1426664175280692 1107 5 0.14804008660171064 1107 6 0.12604689475347897 1107 7 0.3837632580137438 1107 8 0.30429674880019064 1107 9 0.3337962703470936 1107 10-11 0.2253197787583403 1107 12-13 0.3033217981320533 1107 14-15 0.2214503821252194 1107 16-17 0.21272918341031044 1107 18-19 0.39106249046791675 1107 20-21 0.48500576146172136 1107 22-23 0.431242685173153 1107 24-25 0.4002130975205418 1107 26-27 0.21358077276247656 1107 28-29 0.26279819488590306 1107 30-31 0.17884418847262396 1107 32-33 0.1250198588470397 1107 34-35 0.2645484292747824 1107 36-37 0.34214766391684037 1107 38-39 0.2195513016883126 1107 40-41 0.13319741300063725 1107 42-43 0.3359908671099845 1107 44-45 0.3788746142374606 1107 46-47 0.24176236769631032 1107 48-49 0.37509896875640436 1107 50-51 0.291370086622031 1107 52-53 0.4445293247476414 1107 54-55 0.4255160622081391 1107 56-57 0.4996225318139551 1107 58-59 0.535277517579388 1107 60-61 0.4586113550783111 1107 62-63 0.5228653022111232 1107 64-65 0.6962457812265015 1107 66-67 0.7369850126681143 1107 68-69 0.6168866038573384 1107 70-71 0.7138381381630694 1107 72-73 0.6199729354074854 1107 74-75 0.8038975950018532 1107 76-77 0.6016520968537442 1107 78-79 0.45844855726261 1107 80-81 0.44113029849325613 1107 82-83 0.8000369838500099 1107 84-85 0.6781565521001482 1107 86-87 0.6108423894083117 1107 88-89 0.7183222767037449 1107 90-91 0.6464440867091668 1107 92-93 0.688109417260609 1107 94-95 0.9089062172195383 1107 96-97 0.8722799845916711 1107 98-99 1.1406911930465107 1107 100-101 1.4883453492849767 1108 1 -0.0021372793581697636 1108 2 0.16084722791734762 1108 3 0.10175668280402661 1108 4 0.250436613573747 1108 5 0.2711545140649321 1108 6 0.13259143460508227 1108 7 -0.0634983607687758 1108 8 0.18425289257549338 1108 9 0.3040798347941376 1108 10-11 -0.04529555203658475 1108 12-13 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50-51 -2.489847598995766 2316 52-53 -3.010897000951445 2316 54-55 -3.1446448746225357 2316 56-57 -2.46516051975955 2316 58-59 -2.8458161405830182 2316 60-61 -2.105939705381335 2316 62-63 -1.837874155455161 2316 64-65 -2.355656720242898 2316 66-67 -2.249641938647372 2316 68-69 -2.7201332255924555 2316 70-71 -2.685729135524184 2316 72-73 -2.2906479739279426 2316 74-75 -2.0627129936553565 2316 76-77 -1.9283933051645654 2316 78-79 -2.6202657910702456 2316 80-81 -2.0744688935849247 2316 82-83 -1.9180624323741746 2316 84-85 -1.79765734694449 2316 86-87 -2.5622194948128048 2316 88-89 -2.785507257769911 2316 90-91 -2.6672152462323275 2316 92-93 -3.034441663123129 2316 94-95 -3.3169305945277046 2316 96-97 -4.503630919862356 2316 98-99 -5.580216897129915 2316 100-101 -6.129290209237055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5734.0 3 2293.0 4 2340.0 5 2651.0 6 2723.0 7 3342.0 8 4105.0 9 5114.0 10 6322.0 11 7649.0 12 8985.0 13 10265.0 14 11618.0 15 13333.0 16 14735.0 17 17529.0 18 16518.0 19 14242.0 20 15182.0 21 16948.0 22 18415.0 23 21024.0 24 24369.0 25 27808.0 26 33124.0 27 38660.0 28 45485.0 29 54888.0 30 66448.0 31 81281.0 32 100648.0 33 128321.0 34 168599.0 35 226423.0 36 312407.0 37 399109.0 38 293617.0 39 38620.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.151840823315474 17.911447571599865 14.250330733187914 23.686380871896752 2 15.601465423153988 19.111199002287677 40.16026572629007 25.12706984826827 3 20.262295807204396 22.516827587732397 29.052678146808674 28.168198458254533 4 13.082915254447302 15.629357523716214 36.73801555133973 34.549711670496755 5 14.412837609689543 36.43947597727636 34.19397408386081 14.95371232917329 6 33.335737695051996 34.64312044259136 17.164177783520714 14.856964078835924 7 29.632391304059226 31.43806418980364 20.07583082680648 18.853713679330657 8 26.405566556412268 34.25286237375134 19.438521189862495 19.903049879973896 9 25.66033310913661 17.0946759177316 20.2978866508549 36.9471043222769 10-11 25.62050417416201 25.591783992370786 27.033014933166942 21.754696900300257 12-13 26.30960385378093 22.865728532169747 27.37440265886636 23.45026495518296 14-15 23.012723551523738 24.700871208526113 25.698715698058628 26.587689541891525 16-17 22.777207061676943 27.704764713652324 26.353071014365455 23.164957210305282 18-19 22.60057514817356 27.002115927515487 27.960666715196776 22.436642209114176 20-21 23.459969048649622 25.777206455946523 27.861722619368063 22.901101876035796 22-23 23.02400998382848 25.67467043699821 27.935829747588485 23.365489831584828 24-25 23.19956457496187 25.69646885067739 28.026063576744214 23.07790299761653 26-27 22.89416999515849 25.797837223092294 28.286713804849338 23.021278976899872 28-29 22.615020578270524 26.54312700803735 27.951083673908556 22.890768739783574 30-31 22.903139692044462 25.890444195306415 27.77076048018221 23.435655632466908 32-33 22.76914945025052 25.886471728512333 28.00894815004849 23.335430671188657 34-35 23.389152756044833 25.92432695444467 27.678685370209617 23.007834919300883 36-37 23.12680947062271 25.758103221382996 28.089176905127893 23.0259104028664 38-39 22.889035258646125 26.150770778324873 27.91084990646442 23.04934405656459 40-41 23.11534128581014 26.098758562609497 27.783348453035522 23.00255169854484 42-43 22.839674634206755 26.09656145503441 27.340833495514943 23.722930415243894 44-45 23.054628850464262 25.890445950025377 27.49404813902751 23.56087706048285 46-47 23.426835948721774 26.25150853770974 27.350637350962447 22.971018162606047 48-49 23.32183773828259 26.13878929578312 27.313184416944658 23.226188548989633 50-51 22.8256222957186 26.415503680531828 27.625363104893776 23.1335109188558 52-53 23.159917056716537 26.60572128190543 26.896345665426114 23.338015995951913 54-55 22.892601482653827 26.206754547784833 27.185879335714112 23.714764633847228 56-57 23.18662700036496 26.308468165581004 26.972274139856893 23.532630694197145 58-59 23.593476780926657 26.17205660463324 26.94561285782091 23.288853756619186 60-61 23.2477483514894 26.231124570206184 27.522949983566868 22.99817709473755 62-63 22.925369912602804 26.347455436100216 27.423880486763252 23.303294164533728 64-65 22.79438433040469 26.71038989989774 27.104305918736355 23.390919850961215 66-67 22.970492619782153 26.39501366698817 26.836462139932532 23.798031573297145 68-69 22.878996332685986 26.31193845636604 27.194327828675828 23.614737382272143 70-71 23.250318308916565 26.11524231397589 26.968135491560307 23.666303885547237 72-73 23.06137410860308 26.143205147627164 27.32572630396345 23.469694439806315 74-75 22.781404022322747 26.81286056953344 26.91339873400645 23.492336674137366 76-77 23.02245036525247 26.340818262844657 26.88267024657583 23.75406112532705 78-79 23.019321769701065 26.341005659143846 26.983597594797516 23.656074976357573 80-81 23.30252896880541 26.304323352713325 26.81123183028707 23.581915848194193 82-83 23.241927372114738 26.280113254100286 27.123857185889506 23.35410218789547 84-85 23.066391034553842 26.46457577324673 26.890521172076454 23.57851202012297 86-87 23.04730453583204 26.556795432409263 26.888028032579648 23.50787199917905 88-89 22.92659418664777 26.445033331696717 26.622915911942926 24.00545656971259 90-91 22.897785641224118 26.21769828324994 26.885843032095764 23.998673043430184 92-93 23.17886815089021 26.33677662460723 27.096951999331132 23.38740322517143 94-95 22.945648512494103 26.243421959160795 27.106007064793193 23.70492246355191 96-97 22.485806230440023 26.29508885620445 27.47809835557964 23.741006557775883 98-99 22.621312793170464 26.48296752324903 26.817396967019803 24.078322716560706 100-101 22.62575097497325 26.137092699226834 26.96072054660447 24.276435779195438 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 60.0 1 48.0 2 58.5 3 88.0 4 86.0 5 88.0 6 158.5 7 321.5 8 549.0 9 712.5 10 704.0 11 685.5 12 769.5 13 874.0 14 972.0 15 1102.5 16 1228.0 17 1342.5 18 1506.5 19 1715.0 20 1983.5 21 2302.5 22 2761.0 23 3505.0 24 4506.0 25 5742.0 26 7552.0 27 9987.5 28 12564.0 29 16037.0 30 20216.5 31 24655.0 32 29559.5 33 33604.0 34 37323.0 35 41433.0 36 46675.0 37 51483.5 38 54709.5 39 57744.5 40 61518.0 41 65990.5 42 69441.5 43 72905.0 44 76713.0 45 80683.5 46 84221.0 47 92229.0 48 105962.5 49 105545.5 50 93215.5 51 87472.0 52 86433.0 53 85044.0 54 83125.5 55 78359.5 56 71740.5 57 65645.0 58 60278.0 59 53434.5 60 44795.0 61 37260.5 62 29978.0 63 23437.0 64 17074.0 65 12033.0 66 9134.5 67 7346.5 68 5824.0 69 4508.0 70 3433.5 71 2479.0 72 1804.5 73 1312.5 74 942.5 75 719.0 76 532.0 77 315.0 78 190.5 79 123.0 80 85.0 81 58.5 82 35.0 83 30.5 84 24.5 85 18.0 86 10.0 87 7.0 88 6.5 89 5.5 90 4.0 91 5.0 92 6.0 93 3.0 94 1.5 95 1.5 96 1.0 97 1.0 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03352664970577932 2 0.02286712123731914 3 0.020478679173846735 4 0.050732278644497204 5 0.053739946428129126 6 0.048918831892601496 7 0.04958228802134383 8 0.0425938834652579 9 0.04281503550817201 10-11 0.050997661095994136 12-13 0.04323522438970882 14-15 0.03832564903701555 16-17 0.04672942666775178 18-19 0.049648633634218065 20-21 0.010770104489917236 22-23 0.019129651712070657 24-25 0.0047989993312362225 26-27 0.010150878769757725 28-29 0.005727837911475491 30-31 0.009045118555187165 32-33 0.0037816999338313086 34-35 0.007762436706285318 36-37 0.009421077028141156 38-39 0.011101832554288402 40-41 0.0018134467518957154 42-43 0.0021230596119754717 44-45 0.001791331547604304 46-47 2.874976557883451E-4 48-49 9.730689888220912E-4 50-51 2.432672472055228E-4 52-53 0.0029192069664662735 54-55 0.006391294040217825 56-57 0.016055638315564502 58-59 0.009951841931135023 60-61 0.009332616210975512 62-63 0.005197073008481623 64-65 0.0 66-67 0.006568215674549115 68-69 0.016719094444306838 70-71 0.004975920965567511 72-73 0.001791331547604304 74-75 0.0024326724720552277 76-77 0.005440340255687146 78-79 0.005396109847104324 80-81 0.00988549631826079 82-83 0.005794183524349725 84-85 0.01711716812155224 86-87 0.003980736772454009 88-89 0.005617261890018435 90-91 0.0033393958480030855 92-93 0.01579025586406757 94-95 0.010681643672751592 96-97 0.009421077028141156 98-99 0.020832522442509317 100-101 0.03653431748941124 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2260888.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.51389178248302 #Duplication Level Percentage of deduplicated Percentage of total 1 79.68319435701429 50.609897832739684 2 12.213032806410691 15.51394488004567 3 3.6860995508505945 7.023555839165518 4 1.5875786796574147 4.033332018237532 5 0.8021199087127092 2.5472878539277084 6 0.4907677915304543 1.870234344095608 7 0.31528136804824897 1.401732268387478 8 0.2096321783231488 1.0651644390514126 9 0.1527745888234505 0.8732977831481378 >10 0.7997492834934492 8.966213259067137 >50 0.04080404694870471 1.7799576748948585 >100 0.01673721632951355 1.8786624199901052 >500 0.0010444799331273957 0.45663334978973175 >1k 0.0010444799331261036 1.4262439610029543 >5k 1.3926399108348046E-4 0.5538420764563241 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 7193 0.318149328936241 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5330 0.235748077746443 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 3892 0.1721447502043445 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3708 0.16400635502510516 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3470 0.15347951778239347 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3161 0.13981232153030138 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2478 0.10960295246823372 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.846081716564464E-5 0.0 0.0 0.0 0.0 7 8.846081716564464E-5 0.0 0.0 4.423040858282232E-5 0.0 8 8.846081716564464E-5 0.0 0.0 4.423040858282232E-5 0.0 9 8.846081716564464E-5 0.0 0.0 4.423040858282232E-5 0.0 10-11 1.1057602145705581E-4 0.0 0.0 4.423040858282232E-5 0.0 12-13 1.3269122574846698E-4 0.0 0.0 4.423040858282232E-5 1.3269122574846698E-4 14-15 1.3269122574846698E-4 0.0 0.0 4.423040858282232E-5 1.5480643003987812E-4 16-17 2.2115204291411162E-4 0.0 0.0 4.423040858282232E-5 1.769216343312893E-4 18-19 2.2115204291411162E-4 0.0 0.0 4.423040858282232E-5 1.769216343312893E-4 20-21 2.2115204291411162E-4 0.0 0.0 8.846081716564464E-5 1.9903683862270045E-4 22-23 2.2115204291411162E-4 0.0 0.0 1.769216343312893E-4 2.2115204291411162E-4 24-25 2.2115204291411162E-4 0.0 0.0 2.6538245149693396E-4 2.2115204291411162E-4 26-27 2.2115204291411162E-4 0.0 0.0 4.865344944110456E-4 2.432672472055228E-4 28-29 2.2115204291411162E-4 0.0 0.0 8.403777630736242E-4 2.6538245149693396E-4 30-31 2.2115204291411162E-4 0.0 0.0 0.0017471011390214819 2.6538245149693396E-4 32-33 2.2115204291411162E-4 0.0 0.0 0.0033836262565859077 3.096128600797563E-4 34-35 2.2115204291411162E-4 0.0 0.0 0.0045778472883221105 3.096128600797563E-4 36-37 2.2115204291411162E-4 0.0 0.0 0.00685571333033746 3.096128600797563E-4 38-39 2.2115204291411162E-4 0.0 0.0 0.010040302748300669 3.538432686625786E-4 40-41 2.2115204291411162E-4 0.0 0.0 0.014374882789417256 3.538432686625786E-4 42-43 2.2115204291411162E-4 0.0 0.0 0.02034598794809827 3.538432686625786E-4 44-45 2.2115204291411162E-4 0.0 0.0 0.026494014741110572 3.980736772454009E-4 46-47 2.2115204291411162E-4 0.0 0.0 0.03735258004819345 4.4230408582822324E-4 48-49 2.2115204291411162E-4 0.0 0.0 0.049339020774138306 5.307649029938679E-4 50-51 2.2115204291411162E-4 0.0 0.0 0.05670338380317822 5.749953115766902E-4 52-53 2.2115204291411162E-4 0.0 0.0 0.06610234562702796 5.749953115766902E-4 54-55 2.2115204291411162E-4 0.0 0.0 0.08410412192023664 5.749953115766902E-4 56-57 2.2115204291411162E-4 0.0 0.0 0.11407022373509879 5.971105158681014E-4 58-59 2.2115204291411162E-4 0.0 0.0 0.14571708107610815 6.192257201595126E-4 60-61 2.6538245149693396E-4 0.0 0.0 0.17984084129775557 6.192257201595126E-4 62-63 3.096128600797563E-4 0.0 0.0 0.21513670734684778 6.634561287423349E-4 64-65 3.096128600797563E-4 0.0 0.0 0.25432484935122834 6.85571333033746E-4 66-67 3.096128600797563E-4 0.0 0.0 0.31242149102476546 7.076865373251571E-4 68-69 3.096128600797563E-4 0.0 0.0 0.3879227984756432 7.076865373251571E-4 70-71 3.3172806437116746E-4 0.0 0.0 0.4285705439632569 7.298017416165683E-4 72-73 3.980736772454009E-4 0.0 0.0 0.48197876232701486 7.740321501993907E-4 74-75 3.980736772454009E-4 0.0 0.0 0.5425965372897729 7.961473544908018E-4 76-77 3.980736772454009E-4 0.0 0.0 0.6085661916910523 7.961473544908018E-4 78-79 3.980736772454009E-4 0.0 0.0 0.6856376786466203 7.961473544908018E-4 80-81 3.980736772454009E-4 0.0 0.0 0.8000617456503816 8.182625587822129E-4 82-83 3.980736772454009E-4 0.0 0.0 0.928418391357732 8.846081716564465E-4 84-85 3.980736772454009E-4 0.0 0.0 1.1149159091472023 8.846081716564465E-4 86-87 3.980736772454009E-4 0.0 0.0 1.3775781905162927 9.067233759478576E-4 88-89 3.980736772454009E-4 0.0 0.0 1.668901776647052 9.5095378453068E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6715 0.0 23.422415 1 TACATGG 6870 0.0 22.410791 2 ACATGGG 6915 0.0 21.783918 3 GTGTAGC 1435 0.0 19.867767 1 GTCTACG 290 2.4319888E-9 19.662237 1 GAGTACT 5340 0.0 19.624289 12-13 GTACTTT 5465 0.0 19.131521 14-15 GGACCGT 335 7.2759576E-10 18.440687 6 AGAGTAC 8560 0.0 18.319511 10-11 GAGTACA 5440 0.0 18.168293 1 GTATATA 1205 0.0 18.139297 1 AGTACAT 4980 0.0 17.557285 2 TACCTGG 1655 0.0 17.51462 2 CATGGGG 4725 0.0 17.400482 4 GTACCTG 1925 0.0 17.278934 1 AGTACTT 5615 0.0 17.055006 12-13 GTATAGG 575 0.0 16.527678 1 TAGCCCT 1470 0.0 16.48807 4 GTAGCCC 1315 0.0 16.261314 3 GTATAGC 830 0.0 16.029858 1 >>END_MODULE